Food Science & Nutrition
·
2021
·Vol. 9
·期 6
·3059-3070
·
IF 2.863
Hongliang Yao,Jiani Yang,Jiajia Zhan,Qu Lu,Min Su,Yaojiong Jiang
To improve the utilization of okra seed, acidic and enzymatic hydrolyses of producing protein hydrolysates were respectively optimized by orthogonal experiment and response surface methodology using the degree of hydrolysis (DH) as evaluating index. Amino acid composition and antioxidant capacity in vitro of two kinds of hydrolysates were both analyzed. The degree of acidic hydrolysis was 58.53 ± 1.92% under the following optimized condition: hydrolyzing time 40 hr, temperature 95°C, ratio of acid solution to okra seed meal (OSM) powder was 5:1 (V:W/ml:g), and hydrochloric acid concentration was 18% (W/W). The degree of enzymatic hydrolysis was 16.26 ± 0.56% under the optimized condition: hydrolyzing time 8.20 hr, ratio of buffer to OSM powder was 10:1, and enzyme dosage was 3,100 International Units (IU) g −1 . Enzymatic hydrolysates had a fuller range of amino acids and antioxidant capacity than acidic hydrolysates. The results provide technical support for the expansion of okra seed utilization.
研究领域:
食品科学
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营养学
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生物化学
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DOI
PubMed
高影响因子
BOTANICA MARINA
·
2024
·Vol. 67
·期 5
·487-511
·
IF 1.6
Matthew N. Noach,Richard L. Mampana,Eugene Van Rensburg,Neill J. Goosen,Robert W. M. Pott
Alginate is a brown seaweed-based linear polysaccharide of D – mannuronic acid and L – guluronic acid residues. Hydrolysis products of the polysaccharide, specifically oligosaccharides, have been receiving increasing interest, due to their significant bioactivity and potential utilisation routes. The bioactivity of alginate oligosaccharides is closely linked to structural characteristics, namely: molecular weight, degree of polymerisation, and ratio of the monomers (the M/G ratio). Hence, potential applications (such as utilisation as a biostimulant fertilizer) depend on these parameters. This review focuses on recent advances in producing alginate oligosaccharides using chemical or enzymatic methods. The literature survey includes utilisation of these methods at both laboratory and industrial scale. For the chemical methods, we assessed the standard laboratory scale procedures of alginate oligosaccharide production, the potential of scaling up to an industrial level, and the subsequent challenges. For the enzymatic route, we provide an overview of alginate lyases and the application perspectives of enzymatic hydrolysis of alginate.
研究领域:
海洋生物学
·
生物化学
·
酶工程
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yue Qiu,Jiayan Xie,Jing Liang,Jianhua Xie,Yi Chen,Xiaobo Hu,Nan Sun,Xiling Wang,Qiang Yu
In this paper, the optimal conditions for extracting dietary fiber-bound polyphenols in Canavalia gladiata seed coat using three hydrolysis methods, namely alkali, acid and enzyme, were obtained using response surface methodology. The total phenolic content of alkali hydrolysis products was 2635.97 μg GAE/g DW, which was much higher than that of the acid (1418.87 μg GAE/g DW) and the enzyme method (1316.39 μg GAE/g DW). Qualitative analysis identified a total of 38 compounds, 33 of which were quantified. The results showed that CGDF-BPs were dominated by phenolic acids, especially gallic acid. Overall the alkaline method hydrolyzed dietary fibers more thoroughly, releasing a higher diversity of compounds. However, their in vitro antioxidant and α-glucosidase inhibitory activities were inconsistent at equivalent concentration due to differences in bound polyphenol composition caused by the alkali, acid, and enzyme methods, which served as a reference for studying of the composition and activity of natural plant polyphenols.
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xinmiao Wang,Qian Wang,Yuanyuan Hu,Fawen Yin,Xiaoyang Liu,Dayong Zhou
The models of rat everted gut sac and hydrolysis by rat plasma were used to clarify the hydrolysis and transport characteristics of tyrosol-SCFA esters (TYr-SEs). HPLC-UV results indicated that TYr-SEs could be hydrolyzed by intestinal lipase, which showed sustained release of SCFAs and TYr. Meanwhile, TYr-SEs and the liberated SCFAs and TYr could cross the membrane and were transported into blood circulation. TYr-SEs were further hydrolyzed by carboxylesterase in plasma. Obviously, the hydrolysis of TYr-SEs in blood also showed sustained release of SCFAs and TYr. Especially, the rates of hydrolysis and transport correlated positively with the acyl chain lengths. Besides, the above rates of the TYr-SE with a straight chain were greater than those of its isomer with a branched chain. Therefore, the above-mentioned two-step release of SCFAs and TYr clearly demonstrated that TYr-SEs would be an effective approach to enhance the beneficial health effects of SCFAs and TYr.
研究领域:
营养学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Kefan Ouyang,Qian Chen,Hexiang Xie,Qin Zhang,Liling Tao,Hua Xiong,Yulan Du,Jiangang Yan,Xinliang Mao,Qiang Zhao
Arthrospira cell residue (ACR) is a byproduct of the spirulina industry that can be valorized into protein hydrolysates. In this study, we used alkaline protease to hydrolyze ACR and evaluated the effects of hydrolysis time on the properties and functionalities of the hydrolysates. We measured the degree of hydrolysis (DH), particle size, fluorescence, UV‒visible absorption, surface hydrophobicity , emulsification , solubility, and antioxidant activity of the hydrolysates. The DH reached a maximum of 10.2% ± 0.6% after 240 min. Hydrolysis also altered the structure and conformation of the ACR protein, as shown by changes in fluorescence, UV-visible absorption, and FTIR spectra. Hydrolysis enhanced the solubility, surface hydrophobicity , and emulsifying activity of the hydrolysates but not the emulsion stability. The hydrolysates also exhibited higher antioxidant activity than ACR. These results indicate that enzymatic hydrolysis can convert ACR into valuable protein hydrolysates.
研究领域:
生物技术
·
食品科学
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DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2019
·Vol. 43
·期 1
·111-122
·
IF 2.371
Li Mingfu,Guo Chenyan,Luo Bin,Chen Changzhou,Wang Shuangfei,Min Douyong
An autohydrolysis pretreatment with different conditions was applied to sugarcane bagasse to compare the impacts of the physicochemical properties and hydrolytic inhibitors on its enzymatic hydrolysis. The results indicate that the autohydrolysis conditions significantly affected the physicochemical properties and inhibitors, which further affected the enzymatic hydrolysis. The inhibitor amount, pore size, and crystallinity degree increased with increasing autohydrolysis severity. Furthermore, the enzymatic hydrolysis was enhanced with increasing severity owing to the removal of hemicellulose and lignin. The physicochemical obstruction impeded the enzymatic hydrolysis more than the inhibitors. The multivariate correlated component regression analysis enabled an evaluation of the correlations between the physicochemical properties (and inhibitors) and enzymatic hydrolysis for the first time. According to the results, an autohydrolysis with a severity of 4.01 is an ideal pretreatment for sugarcane bagasse for sugar production.
研究领域:
生物技术
·
可再生能源
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DOI
PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2017
·
IF 3.348
Xin Li,Zihui Liu,Ying Xu,Dongfeng Wang
A novel heterobinuclear complex [CoNa(C 15 H 10 NO 4 F) 2 (CH 3 OH)] 2 with Schiff base (C 15 H 10 NO 4 F: 2-amino-4-fluorobenzoic acid-3-methoxysalicylaldehyde) was synthesized and characterized by IR and 1 H NMR spectroscopy, elemental analysis and single crystal X-ray diffraction. X-ray crystallography reveals that the cobalt atom is six-coordinated by two nitrogen atoms from –C N–, two carboxylate oxygen atoms and two hydroxyl oxygen atoms in different ligands, while the sodium atom is seven-coordinated by two methoxy oxygen atoms, two hydroxyl oxygen atoms in different ligands, two oxygen atoms in the same carboxylate and one oxygen atom of solvent methanol. The reaction results of the complex with the p -nitrophenylphosphate (pNPP) and the adenosine monophosphate (AMP) reveal that the complex can hydrolyze phosphoester bonds. Then the DNA-hydrolysis activity is studied experimentally and theoretically, indicating that the complex can effectively hydrolyze the pBR322 supercoiled plasmid DNA . The molecular docking technology predicts the best binding site and binding affinity between the complex and DNA, and then the catalytic mechanism of hydrolysis is supposed. The study results suggest that the Schiff base metal complex, as a potent artificial enzyme , may find its applications in catalytic hydrolysis and biotechnological areas.
研究领域:
配位化学
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生物无机化学
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催化化学
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DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 2
·270
·
IF 3.098
Chunlan Tang,Bingchu Wu,Jinyi Wu,Zheng Zhang,Bocheng Yu
Gastrodia elataBlume (G. elata), a traditional Chinese medicine, is widely used for treatment of various neuro dysfunctions. However, its quality control is still limited to the determination of gastrodin. In the present study, two novel strategies based on quantitative evaluation of total gastrodin and gastrodigenin with base hydrolysis and total gastrodigenin with base-enzymatic hydrolysis followed by HPLC-FLD were put forward and successfully applied to evaluate the quality of 47 batches ofG. elatafrom eight localities. Meanwhile, a systematic comparison of the novel strategy with the multiple markers and the Pharmacopeia method was performed. The results showed that the parishins category could be completely hydrolyzed to gastrodin by sodium hydroxide solution, and gastrodin could further utterly hydrolyze to gastrodigenin with β-d-glucosidase buffer solution. The contents of total gastrodin and gastrodigenin ranged from 1.311% to 2.034%, and total gastrodigenin from 0.748% to 1.120% at the eight localities. From the comparison, we can conclude that the two novel strategies can comprehensively reveal the characteristics of overall active ingredients inG. elatafor quality control. The present study provides a feasible and credible strategy for the quality control ofG. elata, suggesting a revision of the latest Chinese Pharmacopoeia or European Pharmacopoeia methods for the modernization ofG. elatause.Keywords:total gastrodin;total gastrodigenin;base-enzymatic hydrolysis;HPLC-FLD
研究领域:
药理学
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分析化学
·
中药学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Yu Tian,Kunlin Li,Fei Wang,Xin Sun,Kai Li,Ping Ning
H 2 O molecules are challenging to dissociate at low temperatures to produce hydroxyl groups for heterogeneous carbonyl sulfide (COS) hydrolysis. Utilizing the inherent hydroxyl groups on Al 2 O 3 surfaces for catalytic COS hydrolysis is effective; however, the relationship between the hydroxyl nature and hydrolysis efficiency remains unclear. In this study, we investigated the effects of crystal structure, surface morphology, specific surface area, and hydroxyl characteristics of Al 2 O 3 on COS hydrolysis efficiency. Results showed that the content, stability, and activity of hydroxyl groups significantly impacted the efficiency. Catalysts prepared from commercial alumina (Al 2 O 3 -C) exhibited superior COS hydrolysis efficiencies under both dry and humid conditions compared to pseudo-boehmite derived Al 2 O 3 (Al 2 O 3 -S) and boehmite derived Al 2 O 3 (Al 2 O 3 -B) owing to the higher, more stable, and active hydroxyl content in Al 2 O 3 -C. Although Al 2 O 3 -S contained a sufficient amount of surface hydroxyl, its lower activity reduced the hydrolysis efficiency. The catalytic COS hydrolysis mechanism over hydroxyl-rich Al 2 O 3 was elucidated through various characterization methods, providing new insights into the correlation between Al 2 O 3 surface hydroxyl properties and COS hydrolysis efficiency.
研究领域:
化学工程
·
催化
·
表面科学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Xueyan Wang,Le Liu,Ruiyu Shen,Qian Wang,Xiaoqi Xie,Weiming Liu,Zhimin Yu,Xianzhen Li,Xiaoyu Guo,Fan Yang
The inefficient decomposition of polysaccharides, particularly branched polysaccharides limits their large-scale industrial applications. Further understanding and modification of glycoside hydrolases (GHs) processivity is expected to overcome this limitation. Here, a novel xanthan-binding CBM ( Mi XBM), which was supposed to alter the processivity of GHs, was systematically characterized. Phylogeny and structure analyses indicated that Mi XBM is closely related to putative polysaccharide side chain-binding modules. Quantitative binding assays further revealed that Mi XBM probably has a high affinity for xanthan side chain via a variable loop site. Moreover, catalytic performance demonstrated that xanthanase chimeras containing Mi XBM promote highly efficient hydrolysis of xanthan because of improved substrate accessibility. Notably, Mi XBM was observed to enhance the processivity of xanthanase, owing to its high substrate affinity to the repeating unit xanthan. Furthermore, sequential hydrolysis of xanthan by xanthanases with varying processivity resulted in significantly increased hydrolytic efficiency and focused oligoxanthans array. These results expand understanding of CBM-substrate recognition and shed light on efficient degradation of other regularly branched polysaccharides using modified GHs.
CAS / MDL:
研究领域:
生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2017
·
IF 1.174
Lei Guo,Yangdong Hu,Lianying Wu,Chen Liang,Weitao Zhang
To achieve green hydrolysis technology of hemicellulose through repeated using hydrolysate, the hydrolysis of hemicellulose in corncob was studied. The influence of repeated use of corncob hydrolysate on concentrations of d -xylose and l -arabinose was investigated. The loss rates of d -xylose in the prepared d -xylose solutions both with and without corncob, and repeated using corncob hydrolysate under identical acidity condition were discussed. The result shows that d -xylose concentration and l -arabinose concentration are all gradually increasing with the growing time of repeated use of corncob hydrolysate. After the fifth repetition, the concentrations of d -xylose and l -arabinose are 196.7 g·L − 1 and 22.0 g·L − 1 , respectively. Substance inhibiting the degradation of d -xylose is generated during repeated use of corncob hydrolysate, and the production is further proved by the change of d -xylose concentration and the loss rate of d -xylose over heating time.
研究领域:
生物技术
·
化学工程
·
可持续化学
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DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2019
·
IF 4.191
Lu Wang,Xue Lin,Jiachao Zhang,Weimin Zhang,Xiaoping Hu,Wu Li,Congfa Li,Sixin Liu
Non-extractable/bound phenolics from plant matrices played an increasingly important role in human health benefits, but conventional solvent extraction missed high amounts of bound phenolics. In this study, Rubus idaeus L. leaves (RDLR) and seeds residues (RDSR) free of extractable phenolics were treated by different methods, respectively. The results revealed that acid, alkaline, and enzymatic hydrolysis can all significant improve the releasing of bound phenolics. Moreover, acid hydrolysis was the most efficient method for releasing the bound phenolics from RDLR and RDSR, followed by enzymatic hydrolysis. The quantitative results showed that most of individual phenolic compounds were significantly enhanced from RDLR and RDSR after acid hydrolysis. Especially for ellagic acid , was increased by 156.1-folds for RDLR and 101.8-folds for RDSR compared with methanol extraction. For RDLR or RDSR, regardless of the hydrolysis methods, higher contents of bound phenolics evidently showed the stronger antioxidant activities and inhibitory potency in α -glucosidase. Importantly, a significantly positive correlation existed in between the investigated bio-activities and bound phenolics/flavonoids released from RDLR or RDSR. These results provided comprehensive approaches for treating the residues of agricultural crops or fruit tree leaves enriched in bound phenolics, thereby to enhance their bio-accessibility.
研究领域:
食品科学
·
植物化学
·
营养学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）