International Journal of Electrochemical Science
·
2023
·
IF 1.5
Liang Bai
Salbutamol, clenbuterol, ractopamine and albuterol have the effect of promoting protein synthesis and increasing muscle mass. Despite that these substances are banned or restricted in animal husbandry in many countries, some farmers still use them illegally to improve profits. Athletes can test positive for stimulants after ingesting food with these drug residues. In this study, the electrochemical behavior of salbutamol, clenbuterol, ractopamine and albuterol were investigated with carbon nanotubes modified glassy carbon electrodes. The linear detection range and detection limit of four stimulants were studied under optimal conditions.
研究领域:
分析化学
·
药物检测
·
食品安全
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ACS Pharmacology & Translational Science
·
2026
·
IF 4
Bai Yajun,Zhao Xiaolong,Wang Fei,Liu Dan,Diao Yujie,Sun Ying,Zhao Yingying,Zhang Liyuan,Bai Yujun,Wang Shixiang,Jia Pu,Zhao Ye,Zheng Xiaohui
Acorus tatarinowii , a
traditional
Chinese medicinal herb, is used clinically to treat neurological and
respiratory disorders. Our previous work showed that α-asaronol
(α-AOL), a low-toxicity natural constituent of A. tatarinowii , exerts robust antiepileptic effects
through γ-aminobutyric acid (GABA)ergic modulation. Given the
critical role of the peripheral GABAergic system in asthma pathogenesis
and inflammation regulation, the therapeutic potential of α-AOL
for asthma warrants investigation. This study evaluated the efficacy,
safety, and pulmonary targeting of both nebulized and oral α-AOL.
Murine asthma models were established using ovalbumin (OVA) and house
dust mite (HDM). Both nebulized α-AOL (5, 10, 20 mg/kg) and
oral α-AOL were administered, with α-asarone, and albuterol
as references. Histological, biochemical, and functional assessments
were performed to evaluate lung inflammation, mucus secretion, cytokine
levels (IL-4, IL-5, IL-13, TNF-α), airway smooth muscle (ASM)
relaxation, and airway hyperresponsiveness (AHR). Pharmacokinetic
and safety profiles were determined via HPLC tissue distribution analysis,
human ether-à-go-go related gene (hERG) assays, and monitoring
of respiratory and cardiovascular function. Nebulized α-AOL
dose-dependently alleviated OVA/HDM-induced airway inflammation, characterized
by reduced inflammatory cell infiltration, cytokine release, mucus
hypersecretion, and AHR. It also induced concentration-dependent ASM
relaxation and demonstrated dual therapeutic activity by both preventing
and reversing methacholine (Mch)-induced acute bronchospasm. Nebulization
provided sustained lung targeting (≥60 min retention). α-AOL
exhibited negligible hERG inhibition (IC 50 > 100 μM),
no adverse respiratory effects, and no significant impact on blood
pressure. In conclusion, nebulized and oral α-AOL possesses
potent anti-inflammatory and bronchodilatory activities; the nebulized
formulation shows favorable lung-targeted delivery and an excellent
safety profile, positioning it as a promising inhaled candidate for
asthma therapy, particularly in pediatric populations.
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ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jieqing Feng,Qisheng Zhong,Ting Zhou
The online coupling technique of sample preparation with chromatography is a frontier topic in analytical chemistry since it minimizes errors caused by sample loss, shortens analysis time, and reduces solvent consumption. An online pressure change focusing-supercritical fluid selective extraction chromatography (PCF-SFSEC) technique was developed in this study, realizing extraction, purification, separation, and detection in a single run with only microliter-scale samples. The pressure change focusing strategy achieved column-head stacking by decreasing the dissolving capacity of the supercritical fluid, enabling the large volume introduction of extractants into supercritical fluid chromatography without causing peak broadening or distortion. All the extracts could be directly loaded into the chromatography system without split flow. Based on the supercritical fluid selective extraction (SFSE) strategy, the sorbents removed interferences and water from samples, effectively alleviating matrix effects and realizing the direct aqueous sample analysis. The efficiency of online PCF-SFSEC was demonstrated by the enantioselective analysis of 22 chiral drugs in rat plasma, covering eight categories with different pharmacological effects. The entire analysis took 25 min, consuming only 5 μL samples. All analytes in PCF-SFSEC obtained sharp and symmetrical peaks with resolutions higher than 1.0, and 86% had resolutions higher than 1.5. Limits of quantification (LOQs) ranged from 0.0600 to 32.1 μg/L. Recoveries were in the range of 75.8–117.2%. In addition, the developed approach obtained more satisfactory repeatability and significantly reduced matrix effects than conventional methods. The newly established online PCF-SFSEC technique is believed to be a green and powerful tool for the chiral analysis of complex samples.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yalan Bian,Zixuan Zhou,Guanghua Li,Sha Liu,Shuang Li,Zhixian Gao,Weijun Kang
Ractopamine (RAC) is a synthetic β-adrenergic agonist, used for the reduction of fat deposition and increasing muscle conversion. Despite its wide application as a feed additive, it has some serious side effects. Therefore, RAC exists at low concentrations in meat products and is difficult to detect, hence its ultrasensitive detection is significant in protecting human health. Herein, a novel nanozymes-laden bioactive hydrogel based on the [email protected] and DNA hydrogel is developed for ultrasensitive optical detection of RAC. The RAC aptamer acts as a crosslinker and [email protected] is evenly embedded in the DNA hydrogel. The formed hydrogel collapses when the RAC binds to the aptamer, releasing the embedded [email protected] This catalyst is then used in the reaction of hydrogen peroxide (H 2 O 2 ) with tetramethyl benzidine (TMB) to produce color changes that deepen with increasing RAC concentration. The proposed nanozymes-laden bioactive hydrogel has a low detection limit (7.39 ng L −1 ) and a wide detection range (0.01–1000 μg L −1 ) and is of great potential for future practical applications in the ultrasensitive detection of food additives .
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Bin Liu,Chunmiao Bo,Yan Li,Xiaohua Ma,Bolin Gong,Shengwei Guo
High-selectivity and high-exclusion restricted access materials (RAMs) benefit the demands of complex biological samples. In this study, mixed-mode-adsorption RAMs bearing zwitterionic polymer brushes as their outer layers were proposed. The reversed-phase/bronate affinity (RP/BA) mixed-mode adsorption layers on the surface of the silica gel were first formed by surface-initiated atom transfer radical polymerization (SI-ATRP) employing styrene (St) and 4-vinylphenylboronic acid (4-VPBA) as comonomers Afterward, zwitterionic poly(sulfobetaine methacrylate, SBMA) was grafted via another SI-ATRP reaction to establish the external hydrophilic layer. The selectivity of the developed Sil@poly(St-co-4-VPBA)@poly(SBMA) RAMs was examined employing different analytes (benzenes, tetracyclines, neurotransmitters, β-agonists, and their structural analogs), the results revealed the preferential adsorption of substances bearing phenyl and cis-diol groups owing to the multiple interactions (hydrophobic, π-π and BA forces) caused by the RAMs with RP/BA mixed-mode adsorption mechanism. On the other hand, the synergistic effect of the strong-hydrophilicity and high-density zwitterionic poly(SBMA) could efficiently promote the exclusion of RAMs. Moreover, the experimental data revealed that > 99% of bovine serum albumin (BSA, 1 g L –1 ) could be excluded, although the tetracycline (50 µg L –1 ) was completely adsorbed, indicating the maximized adsorption capacity of the RAMs toward small molecules after the efficient exclusion of protein interference. Solid-phase extraction (SPE) employing the developed Sil@poly(St-co-4-VPBA)@poly(SBMA) RAM coupled with high-performance liquid chromatography (HPLC) was successfully employed to determine the tetracycline content of a milk sample. The established method exhibited satisfactory linearity (10–700 µg L –1 ), high recovery (93.1%–108.6%) and good precision (2.6%–8.4%). Finally, our proposed method for synthesizing RAMs could efficiently boost the adsorption selectivity and restricted access function of RAMs, thereby promoting their application in analyzing biological samples.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 11
·1-7
·
IF 5.833
Wang Tingting,Yang Li,Cheng Yuhuan,Zhang Yulian,Ye Jiannong,Chu Qingcui,Cheng Guifang
A novel kind of chiral open-tubular (OT) column was established with homochiral zeolitic imidazolate framework-8 nanomaterials using L-histidine as the chiral carbon center (L-His-ZIF-8). The morphologies of L-His-ZIF-8 nanoparticles and chiral OT column were characterized by scanning electron microscopy. The effects of L-His-ZIF-8 concentrations, pH values, and concentrations of the running buffer on the resolution of the selected chiral compounds were investigated based on miniaturized capillary electrochromatography with amperometric detection system (mini-CEC-AD), respectively. The separation performances of the prepared L-His-ZIF-8@OT chiral columns were explored under the optimal conditions, and the RSDs of run-to-run, day-to-day, and column-to-column reproducibility were less than 6.7% using salbutamol raceme as the model enantiomers. The prepared chiral OT columns have been successfully applied to the enantioseparation of one pair of amino acid enantiomers, two pairs of racemic drugs, and three pairs of neurotransmitter enantiomers. Under the optimum conditions, the prepared OT columns were applied to real-world sample analysis of salbutamol aerosol. The limits of detection of salbutamol raceme were 0.90 μg·mL −1 (S/N = 3), and the recovery was 80.4–82.7%. The assay results indicated that this kind of chiral OT column modified with homochiral L-His-ZIF-8 possesses good reproducibility and stability. This developed mini-OT-CEC-AD system has some attractive characteristics of sensitivity and low cost, providing a potential way for the separation of chiral compounds. Graphic Abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yingjie Zhao,Li Tian,Xin Zhang,Zhuo Sun,Xiangyu Shan,Qian Wu,Ruizhan Chen,Juan Lu
A specificity recognition molecularly imprinted electrochemiluminescence sensor (MIECLS) for determination of clenbuterol (CLB) was established by molecular imprinting technology. In this sensing platform, Fe 2 O 3 was used as the carrier to fix Ru(bpy) 3 2+ , and it could also improve electrochemiluminescence (ECL) signal due to large specific surface area and better electronic transmission efficiency. The introduction of molecular imprinted membranes (MIPs) with CLB as template molecules realized the specificity identification of sensors. The intensity of the ECL signal decreased gradually when the concentration of CLB increased. Based on this characteristic, a novel method for measuring CLB was established, and the related mechanism was studied. Under the optimal conditions, the ECL response was proportional to the logarithm of CLB concentration in the range of 1 × 10 −9 mol L −1 to 1 × 10 −4 mol L −1 with a detection limit of 3.3 × 10 −10 mol L −1 . The results prove that MIECLS has great potential in pork and liver samples testing.
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JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Li Yang,Xiaoshuang Zhao,Yuzhu Chai,Chaodan Li,Yulian Zhang,Li Chen,Jiannong Ye,Qingcui Chu
In this work, novel stationary phase coatings by zeolite SiO 2 NPs coupled with β-cyclodextrin (β-CD) or β-CD/L-phenylalanine were developed for chiral open-tubular capillary electrochromatography (OT-CEC). The OT columns were prepared taking advantage of the strong adhesion of polydopamine in one-step method. Scanning electron micrography and electroosmotic flow were used to characterize the prepared single/dual-selector OT columns. Chiral separation of four chiral analytes (catechin/epicatechin, ephedrine/pseudoephedrine, ritodrine and salbutamol) was carried out in order to evaluate the performance of the prepared columns in OT-CEC with amperometric detection system. In terms of migration time, peak area, resolution, and selectivity factor of catechin/epicatechin and salbutamol, the run-to-run, day-to-day, and column-to-column repeatability were within 8.9%. Under the optimum conditions, the developed methods were applied for the analyses of Chinese herbal medicine Catechu herbs and salbutamol aerosol samples.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Nuo Duan,Shuo Qi,Yucong Guo,Wei Xu,Shijia Wu,Zhouping Wang
Clenbuterol hydrochloride (CLB), a kind of β-agonist, has been illegally used as a feed additive for growth promotion in the food industry in many countries. Herein, surface-enhanced Raman scattering (SERS) combined with aptamer recognition was used for the ultrasensitive and quantitative detection of CLB. Fe 3 O 4 @ [email protected] nanoparticles displaying strong SERS enhancement were synthesized and used as the active substrate. Aptamer against CLB was immobilized on the surface of the substrate to form the capture probe. The complementary DNA (cDNA) was linked to gold nanoparticles (AuNPs) carrying the Raman reporter 4-mercaptobenzoic acid (4-MBA) to form the signal probe. The detection of CLB was carried out by competitive binding between free CLB and cDNA linked on the signal probe with the aptamer immobilized on the capture probe. The SERS intensity was significantly improved by the coupling effect between the Fe 3 O 4 @ [email protected] and AuNPs, resulting in high sensitivity for the quantification assays with a limit of detection of 0.003 ng mL −1 . The recoveries of the CLB from spiked pork samples were 90.7–108.0% and the coefficients of variation (CV%) were 2.2–3.1%, demonstrating the high precision and accuracy of the assay.
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NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 44
·期 3
·1028-1035
·
IF 3.069
Xiaoshuang Zhao,Linzhen Liu,Na Li,Tingting Wang,Yuzhu Chai,Ziyue Yang,Jiannong Ye,Qingcui Chu,Li Chen
Novel open-tubular (OT) columns were prepared for the first time based on zeolite silica nanoparticles (SiO2NPs) as the nano coating materials. The main immobilization factors, including the size and concentration of zeolite SiO2NPs, the content of bovine serum albumin (BSA), heat temperature and time, were investigated to obtain OT columns with good stability and repeatability. Then, the separation performance of the prepared OT columns with/without BSA modifier was explored using capillary electrochromatography with an amperometric detection system. Under the optimum conditions, eight groups of model molecules, including homologues, structural isomers, epimers and enantiomers, underwent baseline separation in the prepared OT columns. The zeolite SiO2NPs-OT columns provided an efficiency of up to 108 200 plates m−1 for 4-chloro-2-methylaniline, and the OT column modified with BSA accomplished the separation and analysis of ephedrine and pseudoephedrine in ephedra herbs. The relative standard deviations of the migration time, peak area and resolution of the selected model analytes were within 8.4% for run-to-run, day-to-day, and column-to-column repeatability. Furthermore, the prepared OT columns could provide acceptable separation performance in a simple running buffer without any organic additives or surfactants, indicating their promising potential for isomer separation.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2018
·Vol. 99
·期 6
·2818-2825
·
IF 2.379
Yangying Sun,Tian Fu,Shuxian Chen,Zhen Wu,Yuxing Guo,Daodong Pan,Ning Gan
Background A novel colorimetric immunosensor was developed for the simple, sensitive and selective detection of ractopamine (RAC) based on using β -cyclodextrin-modified Fe 3 O 4 particles (Fe 3 O 4 @ β -CD) as capture probes and complex platinum colloid nanoparticles (PtNPs-PV) composed of platinum colloid nanoparticles (PtNPs) and polymerase chelate PowerVision (PV) as signal probes. Results PtNPs-PV double catalyzed the chromogenic substrate 3,3′-diaminobenzidine (DAB), which induced changes in the color of DAB and chromogenic absorbance. Incubation temperature, pH and incubation time were systematically optimized and, under optimum conditions, the measured absorbance values showed a linear relationship with the RAC concentrations in the range 0.03–8.1 ng mL −1 . The detection limit was 0.01 ng mL −1 . The sensor exhibited high sensitivity and specificity, as demonstrated by testing structurally similar organic compounds such as salbutamol, clenbuterol and dopamine. The practicality of the developed colorimetric immunosensor was supported by the successful detection of RAC in pork samples with recovery ranging from 94.00% to 106.00%. Conclusion We designed a novel sandwich-type noncompetitive colorimetric immunoassay for the detection of trace levels of RAC in pork. The proposed method can also be used for the detection of toxins in food products via PtNPs-PV amplification. © 2018 Society of Chemical Industry
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RSC Advances
·
2018
·Vol. 8
·期 10
·5536-5541
·
IF 2.936
Shihua Yu,Zhigang Liu,Jianpo Zhang,Hongwei Li,Na Xu,Xin-xin Yuan,Yuqing Wu
To date, great achievements with GC-MS, HPLC-MS, and fluorescence biosensing techniques have been made to detect illegal additives of salbutamol (SAL) in swine meat. However, these methods are not suitable for rapid on-site screening due to either costly instruments or rather complicated and/or time consuming sample pretreatments. Herein, a simple, rapid and ultrasensitive approach based on an azo-coupling reaction and surface-enhanced resonance Raman scattering (SERRS) is presented. By combining with a magnetic SERS substrate, an indirect detection for SAL, with a LOD of 1.0 × 10−11 M (2.39 pg mL−1), was realized. Moreover, a colorimetric method for naked eye detection was successfully carried out for rapid screening of SAL in concentrations higher than 2.09 × 10−5 M (5 μg mL−1). In addition, the proposed method was successfully applied for the rapid determination of SAL in real swine meat. The entire process, including pretreatment, coupling reaction and SERRS detection, was performed within 7 min. Moreover, the SERRS fingerprint band being specific to corresponding functional group guarantees the selectivity for the target molecule. Therefore, the proposed strategy in the present study offers a new way to identify trace amounts of analytes, such as SAL as well as other illegal additives in health-related products and food.
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