Scientific Reports
·
2014
·Vol. 4
·期 1
·1-7
·
IF 5.078
Parhira Supawadee,Zhu Guo-Yuan,Jiang Ren-Wang,Liu Liang,Bai Li-Ping,Jiang Zhi-Hong
Two stereoisomeric cardenolides, uscharin (1) and a new compound, 2′- epi -uscharin (2), were isolated from the latex of Calotropis gigantea (Asclepiadaceae). Their structures were fully elucidated based on their spectroscopic data, X-ray crystallographic data and chemical evidences. Both epimers (1 and 2) exhibited strong inhibitory effects on HIF-1 activity with different magnitudes. Compound 1 showed much more potent activity than 2 and digoxin, a well-known HIF-1 inhibitor. Discrepancy in potencies between 1 and 2 revealed the contribution of a β-configuration of 2′ hydroxyl moiety for HIF-1 inhibitory activity. This is a first report of the activity of HIF-1 inhibition of thiazoline ring-containing cardenolides.
研究领域:
植物化学
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药理学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
·
2020
·
IF 3.569
Meng Shi,Shikun Deng,Yalan Cui,Xuejun Chen,Tong Shi,Liangcai Song,Ruihua Zhang,Yi Zhang,Jianfu Xu,Jingjing Shi,Chen Wang,Liqin Li
Repeated low-level exposure to sarin results to hippocampus dysfunction. Metabonomics involves a holistic analysis of a set of metabolites in an organism in the search for a relationship between these metabolites and physiological or pathological changes. The objective of the present study was to evaluate the effects of repeated exposure to low-level sarin on the metabonomics in hippocampus of a guinea pig model. Guinea pigs were divided randomly into control and sarin treated groups (n = 14). Guinea pigs in the control group received saline; while the sarin-treated group received 0.4×LD 50 (16.8 μg/kg) sarin. Daily injections (a total of 14 days) were administered sc between the shoulder blades in a volume of 1.0 ml/kg body weight. At the end of the final injection, 6 animals in each group were chosen for Morris water maze test. The rest guinea pigs (n = 8 for each group) were sacrificed by decapitation, and hippocampus were dissected for analysis. Compared with the control-group, the escape latency in sarin-group was significantly ( p < 0.05) longer while the crossing times were significantly decreased in the Morris water task ( p < 0.05). Sarin inhibited activities of acetylcholinesterase (AChE) and neuropathy target esterase (NTE) in hippocampus. The AChE activity of hippocampus from sarin-treated groups is equivalent to 59.9 ± 6.4 %, and the NTE activity of hippocampus from sarin-groups is equivalent to 78.1 ± 8.3 % of that from control-group. Metabolites were identified and validated. A total of 14 variables were selected as potential biomarkers. Phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylinositol (PI), Lysophosphatidylethanolamine (LysoPE or LPE)] and sphingolipids (SPs) [sphinganine (SA), phytosphingosine (PSO) and sphinganine-1-phosphate (SA1P)] were clearly modified. In conclusion, repeated low-dose exposures to sarin disrupted the homeostasis of phospholipid and sphingolipid metabolism in guinea pig hippocampus and may lead to a neuronal-specific function disorders. Identified metabolites such as SA1P need to be studied more deeply on their biological function that against sarin lesions. In future research, we should pay more attention to characterize the physiological roles of lipid metabolism enzymes as well as their involvement in pathologies induced by repeated low-level sarin exposure.
研究领域:
神经科学
·
毒理学
·
生物化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Chunxiao Yan,Fenglian Qi,Shuguang Li,Jiayu Xu,Chao Liu,Zihui Meng,Lili Qiu,Min Xue,Wei Lu,Zequn Yan
The indiscriminate use of nerve agents by terrorist groups has attracted attention of the scientific communities toward the development of novel sensor technique for these deadly chemicals. A photonic crystal (PhC) hydrogel immobilized with butyrylcholinesterase (BuChE) was firstly prepared for the sensing of Sarin agents. Periodic polystyrene colloidal (240 nm) array was embedded inside an acrylamide hydrogel, and then BuChE was immobilized inside the hydrogel matrix via condensation with 3-(diethoxyphosphoryloxy)−1,2,3-benzotriazin-4(3 h)-one (DEPBT). It indicated that a total of 3.7 units of BuChE were immobilized onto the PhC hydrogel. The functionalized hydrogel recognized the Sarin agent and then shrunk, thus the diffraction of PhC hydrogel blue shifted significantly, and a limit of detection (LOD) of 10 −15 mol L −1 was achieved.
研究领域:
生物传感
·
化学传感器
·
材料科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jialong Pang,Yali Liu,Chuanfang Zhao,Jiawen Li,Mengting Ran,Baiyi Zu,Xincun Dou
The structural modification of metal–organic frameworks (MOFs) is of vital importance in many fields, especially in sensing with enhanced performance, while the efficient synthesis of functionalized MOF nanoparticles toward small molecule detection remains challenging. Here, a general cellulose nanofibril (CNF)-induced in situ one-step strategy was proposed for the synthesis of dual-ligand-functionalized europium-based MOF (EuMOF@CNF) nanoparticles under an effective regulation of the crystallization kinetics in hydrothermal synthesis. Based on the unique dual-ligand structure, the obtained EuMOF featured a tunable antenna effect and laid a good foundation for fluorescence-sensing materials. Benefiting from the superior self-assembly properties of CNFs and the tunable antenna effect of EuMOF@CNF, flexible sensing films were constructed, showing excellent mechanical properties (72 MPa for stress and 3.8% for strain) and tunable luminescence properties and achieving instant (1 s) and sensitive fluorescence sensing of sarin analogue vapor with a significantly low limit of detection (LOD, 2.8 ppb) and robust selectivity against a wide range of common interferents (>14 types), especially independent of common acids. We believe that this pioneering design of EuMOF with tunable antenna effects would positively advance the development of high-performance MOF-based fluorescent materials and devices.
研究领域:
材料科学
·
化学传感器
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2021
·Vol. 19
·期 9
·485
·
IF 5.118
Ying Yan,Zhoumin Niu,Boyang Wang,Shangge Zhao,Chao Sun,Yuting Wu,Yuying Li,Hao Ying,Hongbing Liu
Dysregulation of cholesterol homeostasis is a major risk factor of atherosclerosis, which can lead to serious health problems, including heart attack and stroke. Liver X receptor (LXR) α and β are transcription factors belonging to the nuclear receptor superfamily, which play important roles in cholesterol homeostasis. Selectively activating LXRβ provides a promising strategy for the treatment of atherosclerosis. Here, we employed atherosclerotic apoE-knockout mice to evaluate the effects of saringosterol, a phytosterol with potent and selective action for LXRβ, which we identified previously in edible marine seaweedSargassum fusiforme. We found that saringosterol treatment reduced the atherosclerotic plaque burden without having undesirable adverse hepatic effects in apoE-deficient mice fed an atherogenic diet. Meanwhile, reduced serum levels of cholesterol, accompanied by altered expression of LXR-regulated genes involved in cholesterol absorption, transport, efflux, excretion, and elimination, were observed in apoE-knockout mice after saringosterol treatment. Together, our study not only establishes saringosterol as an effective cholesterol-lowering and anti-atherogenic phytosterol but also provides insights into the underlying mechanism.Keywords:saringosterol;liver X receptor;cholesterol metabolism;atherosclerosis;Sargassum fusiforme
研究领域:
药理学
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心血管疾病
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海洋生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Li-Hong Su,Hai-Long Qian,Shu-Ting Xu,Cheng Yang,Xiu-Ping Yan
Specific recognition and selective extraction of mycotoxin in environmental and food matrixes is significant to guarantee public health. Covalent organic frameworks (COFs) are promising adsorbents with tailorable functionality, but their low binding affinity and poor selectivity hamper their wide application for selective extraction of trace mycotoxin from complex matrix. Herein, we report calixarene incorporated molecular imprinting on COF to prepare molecularly imprinted calix[4]arene-containing COF (MICOF-CX4) for supramolecular recognition and specific adsorption of citrinin. Calixarene with host-guest chemistry was used as a functional monomer, while amine units with different topologies and function groups were selected to regulate MICOF-CX4 to match with citrinin. The complementary shape and supramolecular interactions of MICOF-CX4 gave highly selective recognition for citrinin. Moreover, MICOF-CX4 with vast accessible surface and plentiful imprinting sites exhibited faster adsorption kinetics and 4-fold higher adsorption capacity for citrinin adsorption than no-imprinted COF-CX4. Combination of MICOF-CX4 based solid-phase extraction with high-performance liquid chromatography-mass spectrometry allowed interference free determination of trace citrinin in real samples with a low detection limit of 0.03 ng mL −1 , good precision of 4.5 % and quantitative recovery of 88.2 %−101.4 %. The cooperative functions of calixarene and molecular imprinting make COF promising adsorbent for specific adsorption of trace targets in complex matrixes.
研究领域:
食品科学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 12
·2577-2585
·
IF 3.286
Qi Fenglian,Yan Chunxiao,Meng Zihui,Li Shuguang,Xu Jiayu,Hu Xiaochun,Xue Min
G-series nerve agents, such as sarin, tabun, and soman, would cause tremendous harm in military and terrorist attacks, so it is necessary to develop a simple method for the rapid and efficient detection of these hazardous substances. We have developed a tunable acetylcholinesterase (AChE)-functionalized two-dimensional photonic crystal (2D PhC) for the detection of a real nerve agent, sarin. In accordance with the 2D PhC previously prepared by our group, the AChE-functionalized 2D PhC was optimized by adjustment of the amount of monomer in the hydrogel, which not only increased the sensitivity of the 2D PhC, with the detection limit decreasing by two orders of magnitude, but also ensured the structural color spanned the whole visible region in the detection range. A linear relationship between the logarithm of the sarin concentration and the particle spacing of the AChE-functionalized 2D PhC was observed from 7.1 × 10 -17 to 7.1 × 10 -4 mol/L. The AChE-functionalized 2D PhC also responded to mimics of G-series nerve agents, including dimethyl methylphosphonate, diisopropyl methylphosphonate, and isodipropyl methylphosphonate, to various degrees. The proposed 2D-PhC hydrogel has potential for low-cost, trace-level, and on-site monitoring of other G-series nerve agents.
Graphical abstract
研究领域:
生物化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Zitao Shen,Zhuo Wang,Pengfei Ye,Lihong Guo,Sheng Peng,Yuefan Liu,Yongsheng Cui,Juan Zheng,Gangfeng Ouyang
Macrocyclic polymer materials exhibit excellent selectivity and adsorption performance in pollutant adsorption due to unique host-guest recognition. Herein, three kinds of calixarene polymers (C4P, C6P and C8P) were synthesized through Sonogashira reaction, and were characterized through 1 H NMR, FT-IR, SEM, and TEM. The water contact angle experiments revealed that three kinds of calixarene polymers were highly hydrophobic, and they all exhibited high enrichment efficiency for weak polar chloro-substituted benzene compounds (chlorobenzene, o -chlorotoluene, p-dichlorobenzene and o-dichlorobenzene) and BTEX (benzene, toluene, ethylbenzene and xylenes). Surprisingly, three types of calixarene polymers also showed satisfactory enrichment performance for polar nitrobenzene compounds (NBCs). Among them, C8P showed the most outstanding enrichment factors for NBCs (825-1913), which was about 2–7 times better than those of the commercial fibers. Using C8P as coating fiber, a sensitive analytical method was further developed based on solid phase microextraction (SPME), coupled with gas chromatography-mass spectrometry (GC-MS). The established analytical method demonstrated low detection limits (0.06–5.08 ng L −1 ), a wide linear range (0.5–1000 ng L −1 ), and good repeatability. The established method was further applied to the quantification of NBCs in environmental water samples, verifying its feasibility.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Yitong Dai,Chongkun Shao,Xinyang Chen,Shu Yan,Yongsheng Guo,Ji Mi,Wenjun Fang
To solve the heat management problem of supersonic aircraft, endothermic hydrocarbon fuels (EHFs) have been developed as both a coolant and a propellant. The pseudohomogeneous catalyst is an efficient way to improve the heat sink of EHFs. In this work, hydrocarbon soluble macrocyclic calixarene compound C-undecyl calix[4]2-(propylsulfanylmethyl)-resonrcinarene (C11–SC3) was synthesized and used as a stabilizer for hydrocarbon-dispersed Pt nanoparticles (Pt@C11–SC3). The morphological characterization results indicate that Pt nanoparticles are encapsulated by the calixarene, which helps the fabrication of ultrasmall Pt@C11–SC3. Ethylcyclohexane (ECH) was selected as a model substrate of EHFs to construct the Pt@C11–SC3/ECH nanofluid system for pseudohomogeneous catalytic cracking. The cracking test of the Pt@C11–SC3/ECH nanofluid was carried out in a batch reactor and a flow reactor under constant volume and pressure conditions. The distribution of cracking products displayed a significant improvement in terms of cracking conversion and a preferred heat absorbed reaction pathway, leading to a higher heat sink. There was a 16% maximum increase in heat sink in the constant pressure cracking tests with the addition of 0.01 wt % Pt@C11–SC3 at 700 °C. It provides a helpful method for heat sink enhancement of EHFs.
研究领域:
化学工程
·
材料科学
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
TOXICOLOGY LETTERS
·
2022
·
IF 4.271
Meng Shi,Ruihua Zhang,Qian Jin,Yalan Cui,Jingjing Shi,Xuejun Chen,Tong Shi,Yi Zhang,Siqing Zhu,Xingxing Zong,Jianfu Xu,Chen Wang,Liqin Li
Sarin was used as a chemical weapon due to its high neurotoxicity and mortality. Subacute sarin induced cognitive and behavioral disorder. However, the underlying mechanism is still unclear. Here we offered a multi-omic approach for the analysis of altered metabolites, lipids, and proteins to explore the neurotoxicity of subacute sarin. Guinea pigs were administered between the shoulder blades 16.8 μg/kg of sarin in a volume of 1.0 ml/kg body weight by subcutaneous injection once daily for 14 days. At the end of the final injection, guinea pigs were sacrificed, and striatum were dissected for analysis. A total of 138 different metabolites were identified in the metabolome analysis. Lipids and lipid-like molecules is the largest group (38.41%). For lipidomic analysis, a total of 216 lipids were identified. In proteomic study, over 4300 proteins were identified and quantified. By integrating these enriched components, we demonstrated that the joint pathways disturbed by subacute sarin mainly involving lipid, purine and pyrimidine metabolism in guinea pig striatum. Overall, this study highlights the powerfulness of omics platforms to deepen the understanding of nerve agents caused neurotoxicity.
研究领域:
毒理学
·
神经科学
·
生物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
AIP Advances
·
2022
·Vol. 12
·期 3
·
IF 1.697
Wu Yao,Kong Lingce,Zhang Xiuling,Guo Yueting,Sun Yaxin,Zhao Chonglin,Chen Wenming,Zuo Yanjun,Li Congju
Protection against harmful chemical compounds is a major social concern, especially chemical warfare agents (CWAs) that are easy to synthesize and can cause mass casualties. The metal oxides with large surface area, porous structures, and a large number of active sites have shown outstanding performance for the adsorption and decontamination of CWAs. However, single-component metal oxides show a lower degradation rate for chemical warfare agents. Here, we demonstrate a convenient homogeneous hydrolysis method for the synthesis of mesoporous manganese-based multi-component metal oxides. Our strategy enables Ce or/and Zr to be doped into the δ-MnO 2 structure during the forming process, remarkably enlarging the surface area and providing rich active sites for CWA catalysis. The as-synthesized multi-component metal oxides exhibit excellent performance for the CWA degradation. It is surprising to find that the best degree of removal of mustard gas (HD), soman (GD), and the VX nerve agent (VX) is 90.60%, 87.32%, and 100%, respectively, reaction with multi-component samples in 4 h, 2.92-fold increase to HD, 8.28-fold increase to GD, and 1.67-fold increase to VX contrast with undoped δ-MnO 2 . This work may provide an avenue for developing new generation chemical warfare agent decontamination materials and other catalysts.
研究领域:
材料科学
·
化学工程
·
环境科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Dianfa Zhao,Jie Liu,Yunshan Zhou,Lijuan Zhang,Yuxu Zhong,Yang Yang,Baoquan Zhao,Mengru Yang,Yong’an Wang
It is very important to establish a sustained-release pralidoxime chloride (2-PAM) drug system with brain targeting function for the treatment of neurotoxicant poisoning. Herein, Vitamin B1 (VB1), also known as thiamine, which can specifically bind to the thiamine transporter on the surface of the blood–brain barrier, was incorporated onto the surface of MIL-101-NH2(Fe) nanoparticles with a size of ∼100 nm. Pralidoxime chloride was further loaded within the interior of the above resulted composite by soaking, and a resulting composite drug (denoted as 2-PAM@VB1-MIL-101-NH2(Fe)) with a loading capacity of 14.8% (wt) was obtained. The results showed that the drug release rate of the composite drug was increased in PBS solution with the increase of pH (2–7.4) and a maximum drug release rate of 77.5% at pH 4. Experiments on the treatment of poisoning by gavage with the nerve agent sarin in mice combined with atropine revealed that sustained release of 2-PAM from the composite drug was achieved for more than 72 h. Sustained and stable reactivation of poisoned acetylcholinesterase (AChE) was observed with an enzyme reactivation rate of 42.7% in the ocular blood samples at 72 h. By using both zebrafish brain and mouse brain as models, we found that the composite drug could effectively cross the blood–brain barrier and restore the AChE activity in the brain of poisoned mice. The composite drug is expected to be a stable therapeutic drug with brain targeting and prolonged drug release properties for nerve agent intoxication in the middle and late stages of treatment.
研究领域:
药学
·
纳米技术
·
神经科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）