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Food Science & Nutrition · 2022 ·Vol. 11 ·期 3 ·1212-1222 · IF 3.553

Phenolic compounds, antioxidant activity and sensory evaluation of sea buckthorn (Hippophae rhamnoides L.) leaf tea

Qian He,Kailin Yang,Xinyan Wu,Chunhong Zhang,Chunnian He,Peigen Xiao

Sea buckthorn leaf tea, an emerging potential functional beverage product, has not yet had appropriate product standards and corresponding quality evaluation methods, and its poor taste directly affects the acceptance of the population, thus limiting its market consumption potential. In this study, two major packaging forms of sea buckthorn leaf tea available in the Chinese market were selected. The contents of total phenolics, total flavonoids, and 10 phenolic compounds, as well as the in vitro antioxidant capacity and sensory characteristics of sea buckthorn leaf tea were analyzed. Results showed that the quality of sea buckthorn leaf tea in the Chinese market varied widely. The total phenolic content, total flavonoid content, antioxidant activity, and consumer acceptance of bagged sea buckthorn leaf tea were higher than those of bulk sea buckthorn leaf tea. Multifactorial statistical analysis showed that the taste astringency of sea buckthorn leaf tea was closely related to ellagic acid and isorhamnetin-3-O-neohesperidin. Furthermore, isorhamnetin-3-O-neohesperidin had a greater effect on the antioxidant activity of sea buckthorn leaf tea. Therefore, ellagic acid and isorhamnetin-3-O-neohesperidin can be used as potential quality markers for sea buckthorn leaf tea. This work provides a reference for taste improvement and quality control of sea buckthorn leaf tea.

CAS / MDL: 64-18-6
研究领域: 食品科学 · 营养学 · 农业化学
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FITOTERAPIA · 2023 · IF 3.4

Basic characterization, antioxidant and immunomodulatory activities of polysaccharides from sea buckthorn leaves

Yang Liu,Limei Ran,Yahong Wang,Peng Wan,Hongli Zhou

The polysaccharides from Sea buckthorn leaves (SBLPs) were extracted by hot water and purified by DEAE cellulose , then separated into six polysaccharides (SBLP-S) by DEAE-52 column. Six separated polysaccharides were characterized by Ultraviolet Spectroscopy, Infrared Spectrum, High Performance Liquid Chromatographic and Congo red analysis. The antioxidant activity and immunological activity were investigated in vitro . The results revealed that the monosaccharide composition of SBLP-S-1, SBLP-S-2, SBLP-S-3, SBLP-S-5 and SBLP-S-6 contained Man , GlcN , Rib , Rha , GluA , GalA , Glu, Gal, Xyl , Ara and Fuc , among them, rare glucosamine was found. And SBLP-S-4 contained all above components except GlcN and GluA. FT-IR showed that SBLP-S were sulfated polysaccharide containing uronic acid . Molecular weights of SBLP-S were 338.659, 401.305, 599.849, 393.904, 626.895 and 176.862 kDa. The Congo-red test indicated that SBLP-S-2, SBLP-S-4, SBLP-S-5, and SBLP-S-6 had triple helix conformation. Crude polysaccharides had the strong scavenging activities on DPPH radicals, ABTS radicals and hydroxyl radicals . The six polysaccharides had the activity of immune stimulation on RAW264.7 cell. SBLP-S-2 promoted the phagocytosis best and SBLP-S-6 promoted the NO production best. The results suggested that SBLPs could be used as potential antioxidants and immunomodulatory agents in pharmaceutical and functional food fields.

研究领域: 药理学 · 食品科学 · 生物化学
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Analytical Methods · 2021 ·Vol. 13 ·期 7 ·925-932 · IF 2.896

Preparation and evaluation of photo-responsive hollow SnO2 molecularly imprinted polymers for the selective recognition of kaempferol

Yun Sun,Xiaoying Yin,Lin Zhang,Menghui Cao

Novel photo-responsive hollow structured molecularly imprinted polymers (PHMIPs) were developed as a selective sorbent to recognize and separate analytes in complex samples. The PHMIPs were prepared using kaempferol (KAE) as the template, 4-[(4-methacryloyloxy) phenylazo] benzenesulfonic acid as a photo-responsive functional monomer, and hollow SnO2 (Ho-SnO2) as the support via free radical polymerization. The structure and physical properties of the developed polymers were characterized using different nano structural techniques and spectroscopy. Under alternating irradiation at 365 and 440 nm, the PHMIPs could release and uptake KAE, indicating that the template molecules can be easily bound to recognition sites and released back into solution. From adsorption experiments, the binding properties were evaluated, and the maximal adsorption capacity of the PHMIPs was 11.04 mg g−1. Furthermore, the developed PHMIPs showed high selectivity towards KAE compared to other compounds. Subsequently, the materials were successfully applied to the photo-controlled extraction of KAE from sea buckthorn leaves. The recoveries for KAE were higher than 90% and relative standard deviation values were between 1.81% and 2.53%, indicating the potential of the developed materials for use in extracting KAE from complex samples.

研究领域: 材料科学 · 分析化学 · 药学
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