Metabolomics
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2017
·Vol. 13
·期 12
·1-14
·
IF 3.692
Tang Tian,Gao Qun,Lin Hua,Biville Francis,Xiong Jingyuan,Pei Xiaofang,Zheng Bo,Zou Xiaoli,Wang Chuan
Introduction ClpXP protease is an important proteolytic system in Salmonella enterica serovar typhimurium ( S. typhimurium ). Inactivation of ClpXP by deletion of clpP resulted in overproduction of RpoS and a growth defect phenotype. Only one report has indicated that deleting rpoS can restore the growth of a S. typhimurium clpP mutant to the wild-type level. Whether overproduction of RpoS is responsible for the growth deficiency resulting from clpP disruption and how ClpXP affects the cell metabolism of S. typhimurium remain to be elucidated. Objectives The aim of this study is to investigate the effect of ClpXP on cell metabolism of S. typhimurium and explore the possible co-effect of RpoS associated with ClpXP in cell metabolism. Method We constructed a clpP rpoS double deletion mutant TT-19 (Δ clpP Δ rpoS TT-1) using a two-step phage transduction technique. We then compared the metabolite fingerprints of Salmonella rpoS deletion mutant TT-14 (Δ rpoS TT-1), clpP deletion mutant TT-16 (Δ clpP TT-1), and clpP rpoS double deletion mutant TT-19 (Δ clpP Δ rpoS TT-1) with those of the wild-type strain TT-1 by using gas chromatography coupled with mass spectrometry (GC–MS). Results Deletion of rpoS recovered only a part of the growth of Salmonella clpP mutant. Further metabolome analysis indicated that clpP disruption changed the levels of 16 extra- and 19 intracellular substances, while the extracellular concentrations of 4 compounds (serine, l -5-oxoproline, l -glutamic acid, and l -tryptophan) and intracellular concentrations of 10 compounds ( l -isoleucine, glycine, serine, l -methionine, l -phenylalanine, malic acid, citric acid, urea, putrescine, and 6-hydroxypurine) returned to their wild-type levels when rpoS was also deleted. Conclusion ClpXP affects the cell metabolism of S. typhimurium partially in an RpoS-dependent manner.
研究领域:
微生物学
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分子生物学
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2014
·
IF 2.291
Bei-Bei Wang,Qi Wang,Yong-Guo Jin,Mei-Hu Ma,Zhao-Xia Cai
In this paper, we report a novel strategy for highly sensitive, rapid, and selective detection of Salmonella Enteritidis using two-color quantum dots based on fluorescence resonance energy transfer (FRET) and fluoroimmunoassay technology. In this FRET system, two-color QDs were adopted as donors and acceptors simultaneously to improve its sensitivity and accuracy. The fluorescence intensity of QDs receptors decreased linearly with the increasing concentrations of S. Enteritidis from 75 to 5 × 10 5 CFU/mL due to FRET system and fluoroimmunoassay reaction. The limit of detection of S. Enteritidis in this method was 10 CFU/mL without sample enrichment within 1–2 h. This method has been applied to the analysis S. Enteritidis of synthetic samples and eggshell samples. The QDs receptors even can potentially be developed into a sensor for quantitative or qualitative determination of S. Enteritidis in the further.
研究领域:
食品安全
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微生物学
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分析化学
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纳米技术
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 3
·e70167
·
IF 3.4
Chae-Yeon Woo,Gi-Hyeok Lee,Kyung-Jik Lim,Jun-Won Kang
The use of blue light within a range of 400–470 nm holds significant potential for sanitization purposes. However, due to an extended exposure duration needed for an antibacterial effect, the utilization of a photosensitizer (PS) to increase the efficacy of the treatment becomes essential. This study investigated prospective use of onion peel extract as a PS in combination with 405 nm blue light for the inactivation of Salmonella Typhimurium, a common foodborne pathogen on eggs. Extracts were obtained using 99% ethanol, 50% ethanol, and distilled water (DW). Their photosensitizing activities were then compared. The combination of 405 nm blue light and onion peel extract using 99% ethanol reduced bacterial populations more effectively than blue light treatment alone, while also increasing reactive oxygen species generation, cell membrane permeability, lipid peroxidation, and DNA damage levels. However, the antimicrobial effect of the 99% ethanol extract did not show a concentration dependence. Spraying DW extract on eggshell treated with 99% ethanol onion peel extract at 1 mg/mL and blue light further enhanced Salmonella reduction. Liquid chromatography was conducted for component separation. However, none of the separated fractions exhibited a significant antibacterial effect, suggesting that the active compounds responsible for antibacterial activity might work synergistically in the crude extract rather than individually. In contrast, the crude extract exhibited a significant antibacterial effect, suggesting that 99% ethanol-extracted onion peel can serve as a PS, particularly in its crude state without purification, and effectively inactivate Salmonella on eggshells. Practical Application Antimicrobial blue light (aBL) in the range of 400–470 nm is a promising nonthermal technology with several advantages, including minimal impact on food quality and safety. This study optimized the concentration of onion peel extract to improve its effectiveness as a photosensitizer in aBL treatment against Salmonella Typhimurium on eggshells. These results may serve as a reference for further optimizing aBL treatments, offering a potentially sustainable and cost-effective photosensitizer for pathogen control.
研究领域:
食品安全
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微生物学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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2016
·Vol. 16
·期 3
·2291-2295
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IF 1.338
Liu Daofeng,Yu Zhibiao,Huang Yanmei,Wang Shuying,Wang Jingyun,Guo Qi,Xu Chaolian,Xia Shiqi,Lai Weihua
A competitive lateral flow assay for the rapid detection of Salmonella choleraesuis was developed. Immuno-magnetic nanobeads were produced by covalently coupling anti-Salmonella choleraesuis antibody to magnetic nanobeads. These immuno-magnetic nanobeads were used as visually
detected probes in the subsequent assay. Compared with the traditional sandwich assay, which is used for detecting macro-molecules, this new method was developed based on the competitive relationship between S. choleraesuis in the inspected sample and the outer membrane protein immobilized
on the T line. Thus, only one antibody was necessary in the new assay, whereas a pair of rigorously selected antibodies were required in the sandwich assay. The sensitivity of the competitive assay for S. choleraesuis was 1.2×107 cfu/mL. In addition, no cross reactions were
found in the 17 common non-Salmonella bacteria strains and in the 4 Salmonella strains of other serotypes. Thus, with satisfactory sensitivity and specificity, the assay can be applied for the rapid detection of pre-enriched culture that may contain S. choleraesuis.
研究领域:
微生物学
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生物技术
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食品安全
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诊断检测
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2025
·
IF 5.2
Xiaoxue Yan,Rongfeng Tao,Hongyuan Zhou,Yuhao Zhang,Dong Chen,Liang Ma,Yamin Bai
Purpose Using sanitizer in food industry is an important mean of sterilization and biofilm eradication, but inappropriate operation may lead to resistance, posing a concealed risk to food safety. The purpose of this study was to assess the impact of sub-lethal sanitizers on the biofilm formed by adaptive Salmonella and to explore the variations in transcription within adaptive Salmonella biofilms when co-incubated with sublethal concentrations of sanitizers. Methods The microbroth dilution method was determined to measure the MIC of three sanitizers on Salmonella , and adaptation induction was conducted with steadily increasing sanitizer concentrations. The effect of sub-MIC sanitizers on the biofilm of Salmonella was investigated by crystal violet method, confocal laser scanning microscopy and transcriptomics. Results The results indicated that the maximum growth concentration of the adapted strains was 1.69–43.25 times that of the original MIC, and the number of bacteria and matrix content were increased when re-exposed to sub-MIC benzalkonium chloride (BZK), and the expression of regulatory factors and various amino acid biosynthesis and metabolism-related genes showed an up-regulation trend. Significance This will be beneficial to clarify the correlation and mechanism between the sanitizer adaptation of salmonellae caused by improper sanitization and increased biofilm formation resulting from this adaptation. And it helps to adjust the appropriate dosage of sanitizer and optimize sanitation standard operating procedures (SSOP) in the foodstuff industry, thereby effectively promoting the bactericidal effect and eliminating foodborne pathogens' biofilm.
研究领域:
微生物学
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食品安全
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分子生物学
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DOI
PubMed
高影响因子
Foodborne Pathogens and Disease
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2024
·
IF 1.9
Ji Zeng,Rong Fu,Jiahao Ji,Tong Xing,Dongping Hou,Zefeng Li,Zepeng Zhao,Shaoting Li,Hongmei Zhang
Salmonella enterica is a common foodborne pathogen that poses significant safety risks across the world. And benzalkonium bromide (BK) is widely used as a disinfectant to sterilize the food processing equipment. It has been reported that sub-lethal concentration of disinfectants induced not only the homologous resistance but also cross-resistances. This work analyzed the induced resistances of Salmonella Enteritidis by short-term adaptation (STA) and long-term adaptation (LTA) to BK. We have demonstrated that inefficient sterilization exposes Salmonella Enteritidis to sub-lethal concentrations of BK, and adapts bacteria to a higher minimum inhibitory concentration and minimum bactericidal concentration. In addition, STA, but not LTA, to BK induced heterogeneous resistance to sodium hypochlorite, and cross-resistance to freezing, desiccation, and heating, which may be caused by the membrane composition change of Salmonella Enteritidis. This work could be useful to the optimization of cleaning protocol.
研究领域:
食品安全
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微生物学
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消毒剂研究
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
·
IF 12.2
Shang Wu,Jian Ji,Lina Sheng,Yongli Ye,Yinzhi Zhang,Xiulan Sun
Disinfectants are widely used in food production and environmental sanitation to prevent illness, but bacteria resistance to these disinfectants and co-resistance to antibiotics pose a threat to public health. This study investigated the impact of commonly used disinfectants on the resistance of Salmonella Typhimurium (ST) to disinfectants and antibiotics, and explored the metabolic mechanisms underlying the resistance changes. The results showed that subinhibitory concentrations of disinfectants had a minor impact on the resistance of ST to four disinfectants. However, chlorine-containing disinfectants stress enhanced bacteria resistance to ampicillin, while quaternary ammonium compounds stress increased resistance to tetracycline and gentamicin. Untargeted metabolomics analysis revealed significant changes in glutathione metabolism and lysine and valine degradation pathways after disinfectant exposure. Specifically, ST activated lysine decarboxylation, leading to a significant decrease in lysine levels after benzalkonium chloride exposure, while valine and leucine degradation pathways were activated by sodium hypochlorite stress. The addition of downregulated L-lysine and L-valine increased the sensitivity of ST to antibiotics, providing further evidence for the findings of metabolomics. This study provides guidance for the proper use of disinfectants in food processing and establishes a strategy based on metabolomics to control antibiotic-resistant bacteria.
研究领域:
微生物学
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代谢组学
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食品安全
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公共卫生
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
·
IF 8.5
Liangke Gong,Jianwei Liang,Yu Zhang,Mengyao Zhang,Huan Ao,Tao Yang
Eating food contaminated by foodborne pathogens can lead to illness. The development of electrochemical sensors for pathogen detection has received widespread attention. However, the analytical performance of electrochemical sensors is inevitably affected by the non-specific adsorption of molecules in the sample. Moreover, the external signal probes might be affected by the complex components in the sample accompanied with signal suppression. This work presents an electrochemical aptasensor for Salmonella typhimurium detection based on the self-signal of poly-xanthurenic acid and the antifouling ability of chondroitin sulfate . The detection time was 60 min. The linear range was from 10 1 to 10 7 CFU/mL, and the detection limit was 3 CFU/mL. The biosensors presented good repeatability and storage stability. And the biosensors has been successfully applied in milk and orange juice. This strategy is expected to be applied in the design of other antifouling biosensors, to achieve rapid detection of pathogens and ensure food safety.
研究领域:
食品安全
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生物传感
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微生物检测
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Yafang Wu,Yinglin Wang,Youxue Wu,Zhaoyi Feng,Dezhi Li,Wenjun Zhao,Qing Liu
Background Salmonella, a foodborne pathogen poses significant threats to food safety and human health. Immunochromatographic (ICTS) sensors have gained popularity in the field of food safety due to their convenience, speed, and cost-effectiveness. However, most existing ICTS sensors rely on antibody sandwich structures which are limited by their dependence on high-quality paired antibodies and restricted sensitivity. For the first time, we combined multi-line ICTS strips with fluorescent bacterial probes to develop a label-free multi-line immunochromatographic sensor capable of detecting broad-spectrum Salmonella . Salmonella was labeled with the aggregation-induced luminescence material TCBPE, resulting in its transformation into a green fluorescent probe. Results Using this sensor, we successfully detected Salmonella typhimurium within the concentration range of 10 4 –10 8 CFU/mL with a visual detection limit of 6.0 × 10 4 CFU/mL. Compared to single-line sensors, our multi-line sensor exhibited significantly improved fluorescence intensity resulting in enhanced detection sensitivity by 50 %. Furthermore, our developed multi-line ICTS sensor demonstrated successful detection of 18 different strains of Salmonella without any cross-reaction observed with 5 common foodborne pathogens tested. The applicability and reliability were validated using milk samples, cabbage juice samples as well and drinking water samples suggesting its potential for rapid and accurate detection of Salmonella in real-world scenarios across both the food industry and clinical settings. Significance In this experiment, we developed a TCBPE-based multiline immunochromatographic sensor. Specifically, Salmonella was labeled with the aggregation-induced luminescence material TCBPE, resulting in its transformation into a green fluorescent probe. Through the multi-line analysis system, the detection sensitivity and accuracy of the sensor are improved. In brief, the sensor does not require complex antibody labeling and paired antibodies, and only one antibody is needed to complete the detection process.
研究领域:
食品安全
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微生物学
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生物传感
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Xian Liu,Qiwu Yuan,Yang Yang,Ming Yang,Yuhuan Qing,Xia Li,Xuepin Liao,Bi Shi
Plant polyphenols, particularly lignin-derived polyphenols, exhibit considerable potential in the development of novel antimicrobial agents against multidrug-resistant (MDR) pathogenic bacteria, but their application has been limited by the inherent instability and low bactericidal activity. Here, we developed a high-efficiency and low-resistance strategy for combating MDR bacteria based on gallic acid (GA)- gadolinium (Gd) complex. The antibacterial circle diameters of GA, Gd, and GA-Gd against Salmonella enterica 6819 were 10.50, 16.25 and 24.15 mm, when initial inoculum was 1 × 10 8 CFU/mL, indicating that the combination of GA and Gd synergistically enhances their bactericidal activity. Additionally, GA-Gd showed potent antibacterial activity against two sensitive and ten MDR Salmonella strains with the low minimum inhibitory concentration (0.5 mmol/L) and minimum bactericidal concentration values (1.0 mmol/L). GA-Gd can disrupt bacterial wall, membrane, and DNA, contributing to its efficient antibacterial activity . Notably, the RNA-seq analysis showed that GA-Gd downregulated drug resistance genes related to biofilm formation, cationic antimicrobial peptides resistance, beta-lactam resistance and vancomycin resistance. These results suggest that GA-Gd is a promising antibacterial agent to address the threat of antibiotic resistance and MDR Salmonella infections.
研究领域:
微生物学
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药理学
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植物化学
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产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2025
·
IF 4.3
Linrong Yu,Yunchun Pan,Qingqian Meng,Xianggen Wu,Mengshuang Li
Antibiotic abuse in livestock and poultry farming poses significant threats to human health, including the emergence of drug-resistant bacteria. Thus, antibiotic alternatives are highly required in this field. Thymol (THY) demonstrates significant potential as an antibiotic alternative, but its commercial application is limited due to its high volatility and low water solubility. In this study, a novel nanoformulation of pro-phytomicelles, with glycyrrhizin and rebaudioside A as mixed-nanocarriers that load THY (called “THY@pro-phytomicelles”), was designed and tested as a potent antibiotic alternative. The optimized THY@pro-phytomicelles had an encapsulation efficiency of 99.75 ± 0.37 % and a particle size of 3.83 ± 0.44 nm after they were dispersed in water. Following THY’s formulation into THY@pro-phytomicelles, its water solubility, volatility stability, and antioxidant activity significantly improved. In vitro, the THY@pro-phytomicelles showed better antimicrobial activity than THY. Their antibacterial mechanism appeared to involve destruction of the integrity of the bacterial cell wall and biomembrane, leading to alkaline phosphatase (AKP) leakage and inhibition of biofilm formation. In the animal evaluation in this study, they showed excellent in vivo antibacterial capacities against Salmonella enteritidis -induced enteritis, including improvement of the liver bacterial abscess, alleviation of the oxidative stress and inflammatory cytokine levels, reduction of the bacterial load of the liver, and alleviation of the inflammation and damage caused by bacteria in the cecum. Overall, the THY@pro-phytomicelles showed excellent in vitro and in vivo antibacterial activity, making them a potentially effective antibiotic alternative in livestock and poultry farming, with broad potential application in the breeding industry.
研究领域:
畜牧学
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药剂学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-18
·
IF 12.6
Niu Mengya,Zhao Luo,Gong Shuang,Liu Xinxin,Zheng Cuixia,Jiao Jiannan,Wang Fangfang,Wang Lei
Salmonella Typhimurium ( S . Tm), a Gram-negative pathogenic bacterium, is one of the most common causes of invasive bacterial diseases. Antibiotic therapy remains the principal therapeutic modality for treating S . Tm infection. However, due to the difficulty in precisely targeted pathogenic bacteria after oral administration, the therapeutic effect remains unsatisfactory. Here, we developed an oral probiotic spores-based biohybrid delivery system (BCs@PME-Au) to treat S. Tm-induced colitis. By employing a one-pot metal deposition method, Polymyxin E (PME) acted as a reducing agent to promote the Au 3+ rapid nucleation and growth into PME-capped Au NPs (PME-Au NPs). By forming Au-S and Au-N bonds with the active sites (-SH, -NH 2 ) of Bacillus coagulans spores (BCs), PME-Au NPs were anchored onto the surface of BCs to construct the biohybrid system BCs@PME-Au. Following oral administration, BCs@PME-Au successfully passed through the gastric acid barrier. After absorbed water and nutrients, BCs germinated into Bacillus coagulans (BC) in the gut and PME-Au NPs were released. Based on the BC’s targeting pathogen infection site and PME-Au NPs’ targeting Gram-negative bacteria, the biohybrid system achieved significantly antibacterial effect of S . Tm. Mechanistically, by blocked the LPS-induced inflammatory pathway TLR4/MyD88/NF-κB, BCs@PME-Au exerted a powerful anti-inflammatory effect. With its robust antibacterial efficacy, targeted delivery, and excellent safety profile demonstrated both in vitro and in vivo, the biohybrid system BCs@PME-Au offers significant promise in treating bacterial colitis.
研究领域:
微生物学
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医学
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药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）