TALANTA
·
2017
·
IF 4.162
Le Chen,Junlang Qiu,Yijia Tang,Jianqiao Xu,Shuyao Huang,Yuan Liu,Gangfeng Ouyang
Tetrodotoxin (TTX) is one of the most toxic substances of non-protein in nature. In present study, a rapid and sensitive method based on in vivo solid-phase microextraction (SPME) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed to detect the endogenous TTX in pufferfish ( Fugu ). Fiber evaluation experiments demonstrated that, compared with the commercial PDMS and PA fibers, the home-made electrospun PS@PDA-GA fibers exhibited much better extraction performance towards to TTX in water (120 times and 20 times, respectively), under the optimized conditions. Then, the home-made SPME fibers were employed to extract TTX in spiked homogeneous fish muscle samples, and a LC-MS-MS was used for the analysis. The reproducibilities (RSDs of inter and intra fiber were 12.1%, and 7.9% respectively), linear range (10–1000 ng g −1 , R 2 =0.9963) and sensitivity (the LOD was 2.3 ng g −1 ) of the method were found to be excellent and satisfactory for further in vivo experiments. Especially the LOD of the established method is lower than the National Standard Method of China (GB/T 23217-2008, LOD 50 ng g −1 ). Subsequently, the method was successfully applied to detect the TTX in the dorsal muscle of living pufferfish, and the accuracy was verified with traditional liquid extraction (LE) method. In general, this is the first study to detect TTX in pufferfish by in vivo sampling method, which provided a promising alternative method for the studies of TTX, and also advanced the implementation of SPME for more in vivo studies.
研究领域:
食品科学
·
分析化学
·
毒理学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Chao-Ping Hou,Ke Huang,Qiang Wang,Jin-Lan Yang,Hui-Bing Chen,Ying-Xia Zhang,Qi Liu,Zhi-Guang Li,Hui-Qing Li,Xu-Feng Wang
Tetrodotoxin (TTX), one of the most potent neurotoxins, poses a significant threat to consumer health. This study presents an advanced analytical method combining ion-paired reagent-assisted dispersive solid-phase extraction (ion-paired-dSPE) with hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS), for the quantification of TTX residues in puffer fish. Edible tissue samples were extracted twice using a 1 % formic acid–methanol solution and purified via optimized ion-paired-dSPE procedures. TTX adsorbed onto the magnetic nano-adsorbents was subsequently eluted with an acetonitrile–water–formic acid solution (60:40:1, v/v/v). Detection was achieved through electrospray ionization in positive mode, with quantification performed in multiple reaction monitoring (MRM) mode. Correlation coefficients ( r ) in matrix-matched external standard curves ranged from 0.9995 to 0.9997. The limits of detection (LOD) and quantification (LOQ) in puffer fish tissues were 0.1 μg/kg and 0.3 μg/kg, respectively. Spiked recoveries at three concentrations in negative tissue samples ranged from 73.4 % to 87.5 %, with precision under 10.6 %. The method was validated in accordance with 2002/657/EC guidelines and successfully applied to TTX detection in cultured puffer fish samples.
研究领域:
食品科学
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 9
·1537
·
IF 5.1
Zhihui Liu,Xiaoting Chen,Bei Chen,Yinghong Qu,Haiyan Tang,Ruowen Wu,Kun Qiao,Yongchang Su,Nan Pan,Tingru Chen,Wenzheng Shi,Zhiyu Liu
As a culturally iconic Chinese delicacy, pufferfish lacks systematic research on thermal processing optimization and pre-cooked meal development, limiting its industrial standardization and quality preservation. This study aimed to bridge this gap by evaluating steaming effects onTakifugu flavidusquality. This study systematically evaluated its physicochemical properties and flavor profiles under different steaming durations by determining the water loss rate, mass loss rate, water distribution status, textural properties, color, and free amino acid content using an electronic nose, electronic tongue, and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). The results indicated that the core temperature of the fish meat reached 70 °C after 9 min of steaming. With higher steaming time, its mass loss rate and water loss rate generally increased, though the water loss rate temporarily decreased at 10 min. The mass loss rate stabilized after 12.5 min. The hardness and chewiness of the fish meat increased significantly when steamed for 12.5 min or longer. After 5 min of steaming, the brightness value and yellow-blue value of the fish meat significantly increased, whereas the red-green value significantly decreased. The total free amino acid content showed a fluctuating upward trend and electronic tongue analysis revealed an increase in umami and richness after steaming. Electronic nose and HS-GC-IMS analyses demonstrated that the variety and content of volatile flavor compounds significantly increased with prolonged steaming. Sensory evaluation showed that the 10 min steaming group exhibited better texture and color, while the 15 min steaming group had the best odor. Therefore, the optimal steaming time forT. flaviduswas determined to be 10–15 min. For home cooking, a 15 min steaming process achieves the peak abundance of flavor compounds and the highest sensory evaluation score. For the industrial production of pre-cooked meals, a 10 min steaming process can meet the doneness requirements while maintaining suitable textural properties and color stability. The findings of this study not only advance the scientific understanding of thermal processing effects on pufferfish quality attributes, but also establish a critical technological foundation for developing standardized industrial processing protocols and high-quality pre-prepared pufferfish products.
研究领域:
食品科学
·
水产加工
·
烹饪技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2022
·
IF 3.549
Lei Men,Yuhan Zhang,Keke Li,Zhongyu Li,Chunbin Li,Xueyuan Zhang,Xiaojie Gong,Linlin Fang
As a typical and broad-spectrum benzimidazole, mebendazole (MBZ) has long been used in human and veterinary medicine to treat parasitic infestations, and is widely employed in the aquaculture of Japanese pufferfish (Takifugu rubripes). However, there have been no studies examining the pharmacokinetic characteristics of MBZ in Japanese pufferfish. Furthermore, the presence of MBZ and its metabolites in animal-derived raw food represents a notable safety concern. Here, we investigated the metabolism of MBZ using a UPLC-Q-TOF system. Additionally, we evaluated the pharmacokinetics of MBZ and two metabolites, 2-amino-5(6)-benzoylbenzimidazole (MBZ-NH2) and 5-hydroxymebendazole (MBZ-OH), in Japanese pufferfish following intramuscular injection of 20 mg/kg MBZ. We detected three metabolites of MBZ (M1–M3), among which, 2-amino-5(6)-(a-hydroxybenzyl) benzimidazole (M3) was detected in an aquatic animal for the first time. The plasma dispositions of MBZ, MBZ-NH2, and MBZ-OH were characterized by low plasma clearance, medium distribution volume, and long terminal half-life. Moreover, these compounds were widely distributed in the muscle, from which they were rapidly cleared. The pharmacokinetics and metabolism of mebendazole in Japanese pufferfish are described for the first time in this study. Our findings provide a basis for the rational application of MBZ in Japanese pufferfish farming and contribute to our understanding of the metabolism of MBZ in cultured fish.
研究领域:
水产养殖
·
药物代谢
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·
IF 4.5
Wang Qing,Qiu Mingyu,Yu Shijuan,Yang Xiaomin,Lao Liqin,Wu Zhiwei,Deng Qianjun,Yu Mingguang
Recently, papers with superhydrophobic surfaces and unique antibacterial and self-cleaning capabilities have attracted great attention due to its potential applications in cold chain logistics packaging. Therefore, a facile and versatile route to fabricate superhydrophobic and anti-bacterial adhesion dual-functional paper was reported. The approach was based on pufferfish-like structured ZIF-8 incorporated cellulose fibers. Specifically, cellulose fibers without any chemical pretreatment were used as templates for the in situ aqueous construction of pufferfish-like structured ZIF-8 with zinc nitrate as zinc source and dimethylimidazole as organic ligand. After paper-making and hydrophobicity treatment, the obtained paper possessed excellent superhydrophobic and anti-bacterial adhesion properties. These surfaces retained their water resistance after 30 finger-wipe cycles and 10 sandpaper abrasions. Moreover, the hydrophobic surface prepared also showed excellent anti-adhesion performance toward bacteria. This strategy route proposed here can significantly reduce or eliminate potential risks associated with various contamination.
研究领域:
材料科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
Comparative Biochemistry and Physiology D-Genomics & Proteomics
·
2024
·
IF 2.2
Shengli Fu,Kun Qian,Xiao Tu,Jie Lu,Tuo Yao,Lingtong Ye,Jianmin Ye
The structure of fish intestines does not have a clear regional division, while the function of the intestines may be related to their structure. Therefore, in this study, the delimitation of intestinal segments in pufferfish ( Takifugu obscurus ) was achieved by morphological analysis. Subsequently, enzyme activity, intestinal microbiota, and gene expression were examined to compare the differences among the pufferfish various segments. According to four morphological parameters: height of mucosa folds (HF), width of mucosa folds (WF), thickness of muscularis (TM), and cross-sectional area (CSA), the pufferfish's intestine was divided into anterior intestine (AI), middle intestine (MI), and posterior intestine (PI). The activity levels of amylase, lipase, and trypsin in the AI and MI were significantly higher than these in the PI. According to the analysis of 16S rDNA, the dominant microbiota at the phylum level in the different segments were Epsilonbacteraeota, Spirochaetes, and Proteobacteria. At the genus level, there were variations observed in the relative abundance of Brevinema, Mycobacterium, Bradyrhizobium, and Microvirga. α diversity analysis revealed that the richness indexes (Ace and Chao1) were the lowest in the MI, while β diversity analysis revealed significant difference in intestinal microbial community composition among the three intestinal segments. Furthermore, RNA-Seq was used to identify differential expression genes (DEGs) and biological pathways among the different intestinal segments. The DEGs between the AI and MI were enriched in pancreatic secretion and protein digestion and absorption, those between AI and PI were involved in ascorbate and aldarate metabolism and glutathione metabolism, and those between MI and PI were involved in steroid biosynthesis, fat digestion and absorption, vitamin digestion and absorption, and glycine, serine and threonine metabolism. In conclusion, the presented results compare and analyze the differences in various intestinal segments of pufferfish, which will be conductive to future exploration of the functions of these different segments.
研究领域:
海洋生物学
·
分子生物学
·
微生物学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Yuqian Zheng,Yichen Sun,Yiwen Zhu,Wenli Wang,Imre Blank,Shi Liu,Yin Zhang,Ye Liu,Yuan Liu
Monosodium glutamate (MSG) is a basic representative of umami taste, but it exerts adverse effects as reported in several studies. Enhancing umami perception through aroma-taste interactions is a potentially useful approach as a flavor enhancer to reduce future MSG intake. We identified the aroma compounds in fried Takifugu obscurus liver, then studied the effect of aroma compounds on perceived umami taste using the sensomics approach. A total of 117 volatile compounds were identified from the fried liver. Thereinto, 30 volatile compounds related to five basic tastes were detected by gas chromatography/olfactometry-associated taste analysis. Aroma compounds associated umami, sweet and salty perception in the fried Takifugu obscurus liver showed the potential to enhance umami perception of MSG solutions. Three aldehydes, i.e. heptanal, (Z)-4-heptenal and 2-methylbutanal, significantly enhanced the umami intensity perception of an MSG solution ( p < 0.05). The use of aroma compounds to enhance umami perception is considered as a promising tool, further broadening the research area of umami science for application as flavor enhancers in the food industry. In addition, this study also provides an insight into revealing aroma-taste interaction.
研究领域:
食品科学
·
感官科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 10
·4158
·
IF 4.6
Yichen Wang,Renjin Zheng,Pingping Wu,Youjia Wu,Lingyi Huang,Liying Huang
Tetrodotoxin (TTX) inhibits neurotransmission in animals, and there is no specific antidote. In clinical practice in China,Althaea rosea(A. roseaflower) extract has been used to treat TTX poisoning. In this work, the efficacy of the ethyl acetate fraction extract ofA. roseaflower in treating TTX poisoning in rats was investigated. A high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed to determine nine neurotransmitters in rat brain tissue, including γ-aminobutyric acid (GABA), dopamine (DA), 5-hydroxytryptamine (5-HT), noradrenaline (NE), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxyindole-3-acetic acid (5-HIAA), epinephrine (E), and tyramine (Tyn). The detoxifying effect ofA. roseaflower was verified by comparing the changes in neurotransmitters’ content in brain tissue before and after poisoning in rats. The assay was performed in multiple reaction monitoring mode. The quantification method was performed by plotting an internal-standard working curve with good linearity (R2> 0.9941) and sensitivity. Analyte recoveries were 94.04–107.53% (RSD < 4.21%). Results indicated that the levels of 5-HT, DA, E, and NE in the brains of TTX-intoxicated rats decreased, whereas the levels of GABA, Tyn, and 5-HIAA showed an opposite trend, and HVA and DOPAC were not detected. The levels of all seven neurotransmitters returned to normal after the gavage administration of ethyl acetate extract ofA. roseaflower to prove that the ethyl acetate extract ofA. roseaflower had a therapeutic effect on TTX poisoning. The work provided new ideas for studies on TTX detoxification.Keywords:tetrodotoxin;LC-MS/MS;Althaea roseaflower;neurotransmitters;detoxification
研究领域:
药理学
·
毒理学
·
分析化学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2024
·Vol. 171
·期 2
·027523
·
IF 3.1
Stuart J. Robertson,Jinping Cheng,Minhua Shao
Tetrodotoxin (TTX), a potent neurotoxin, has recently been detected in marine fauna and waters in multiple locations outside its historical range and is projected to increase in both stability and bioavailability in acidifying oceans. We report the first biosensor designed to detect sub-ng ml−1 concentrations of TTX directly in natural seawater. The compact sensor chip employs a competitive enzyme strategy with a glucose oxidase-TTX conjugate that generates an amperometric response in the presence of a ferricyanide redox mediator. The biorecognition layer synthesis is confirmed by attenuated total reflection Fourier transform infrared spectroscopy. Cyclic voltammetry and electrochemical impedance spectroscopy are used to probe the stability and hydrophilicity of the biorecognition layer. We investigate the effects of nonspecific adsorption on sensor results and find that bovine serum albumen is an excellent blocking agent for preventing NSA when incubating with filtered seawater samples. The biosensor has a limit of detection of 0.053 ng ml−1 and a sensitivity of 87 nA ng−1 ml. Requiring no extraction or preconcentration steps with a time-to-results of under an hour, our biosensor provides a new tool for detecting TTX in natural waters, which can lead to more proactive monitoring and higher throughput testing.
研究领域:
海洋生物学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Hongchao Ma,Xifeng Ma,Tiantian Dong,Xinyu Bian,Xiaokang Zhang,Yanhui Wei
Bioinspired nanozymes hold promise for simulating natural processes and creating optimized functional systems, but their application is hindered by limited catalytic activity and selectivity. These challenges can be addressed by reconstructing enzymatic active sites to enhance catalytic efficiency and integrating biological recognition units for specificity. In this work, we developed a peroxidase-mimicking nanozyme by stabilizing hemin on a supramolecular scaffold of diphenylalanine (FF) and glutaraldehyde (GA). To enable specific recognition, we conjugated a tetrodotoxin (TTX) aptamer, yielding the He@FF/GA-Apt composite nanozyme. This nanozyme demonstrated robust catalytic activity in 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation. The TTX aptamer conferred specific TTX recognition, with the aptamer-TTX complex blocking the nanozyme active site and reducing its activity. Based on this mechanism, we created a dual-mode TTX detection method using UV–vis spectroscopy and smartphone RGB analysis. The UV–vis mode achieved a linear range of 1.0–40.0 ng mL −1 and a limit of detection (LOD) of 0.61 ng mL −1 , while the smartphone mode had a LOD of 1.43 ng mL −1 in a linear range of 2.0–40.0 ng mL −1 . Both methods performed well in real samples, with recoveries of 96.29 %–102.57 % (UV–vis mode) and 92.07 %–109.46 % (RGB mode). In comparation, the UV–vis mode offers high sensitivity but requires lab equipment, whereas smartphone RGB mode enables rapid on-site detection despite a little lower sensitivity. This work provides a promising approach for developing target-specific nanozyme sensors.
研究领域:
生物化学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Yajie Tang,Li Yao,Yueliang Wang,Bingyong Lin,Yuanyuan Yao,Lifen Chen,Hong Huang,Jianguo Xu,Longhua Guo
Small-molecule biotoxins are frequently founded in grains, corns, peanuts, and different kinds of aquatic products, and they are harmful to human health. Lateral flow immunoassays (LFIAs) have been widely used for on-site detection of small-molecule biotoxins. However, most of the reported LFIAs approaches are signal-off type because each small-molecule biotoxin only has one antigen binding site due to the small size of the molecule. In this work, we demonstrate a signal-on LFIAs approach for on-site detection of small-molecule biotoxin based on the aptamer/antibody molecular recognition system, and the detection of tetrodotoxin (TTX) was selected as an example. A TTX specific aptamer with high affinity was modified on the gold nanoparticles (AuNPs) to act as the colloid gold labels, and a TTX specific antibody was immobilized on the testing line of the LFIAs to act as the capture antibody. This aptamer/antibody combinations not only allow qualitative screening by the naked eye but also enable semi-quantitative detection when combined with smartphone measurement of RGB values. The detection range was 8–100 ng/mL, with a detection limit of 8 ng/mL, and recoveries ranging from 99.00 % to 110.95 %. The RSD for intra-batch reproducibility was 6.98 %, and the RSD for batch-to-batch reproducibility was 5.20 %. Meanwhile, the proposed LFIAs was successfully demonstrated for the detection of TTX in globefish indicating that the aptamer sensor has good homogeneity for the detection of TTX.
研究领域:
食品安全检测
·
生物传感技术
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Chang’e Wang,Junjian Miao,Chang Liu,Yi Guo,Lili Zhang,Ying Lu
Peptidoglycan (PG) is a key component that enables lactic acid bacteria to remove tetrodotoxin (TTX). However, the adsorption behaviors and interaction mechanisms between PG and TTX remain unclear. Herein, three types of PGs (A3α-PG, A1γ-PG, and A4α-PG) were used to treat TTX. After treatment, the residual TTX content was reduced by 82.85 %–84.73 %, and TTX toxicity decreased by 87.30 %–93.02 %, indicating that the three PGs could effectively remove TTX. Adsorption kinetics analysis showed that the adsorption of TTX by the three PGs conformed to the quasi-second-order ( R 2 > 0.99) and the Freundlich ( R 2 > 0.99) models. These findings suggest that the adsorption behavior between PG and TTX involves a coexistence of chemical adsorption and multilayer physical adsorption modes. Furthermore, molecular docking simulations and quantum chemical calculations clarified that the adsorption interaction between PG and TTX involved physical interactions mediated by hydrophobic interactions and van der Waals forces, as well as chemical interactions mediated by strong hydrogen bonds (1.352 Å) with a Mayer bond order of 0.2687. This study elucidates the mechanism of PG in removing TTX, providing a theoretical basis for developing more efficient TTX adsorbents and detoxification technologies in the future. Further research on the adsorption kinetics and interactions between PG and TTX may offer methodological references and novel insights into the adsorption mechanisms of other biological molecules.
研究领域:
微生物学
·
生物化学
·
食品安全
·
生物物理学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）