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BMC PLANT BIOLOGY · 2025 ·Vol. 25 ·期 1 ·1-17 · IF 4.8

Unveiling transfer RNA modifications of oil palm and their dynamic changes during fruit ripening

Deng Dehai,Qin Yichao,Lin Xiuying,Chu Meng,Lv Daizhu,Lin Huan

Background The oil palm ( Elaeis guineensis ) is a crucial agricultural commodity, yielding the highest oil output among oil-bearing crops. Despite its significance, productivity challenges persist due to genetic and environmental factors. This study breaks new ground by mapping tRNA modifications in oil palm, exploring their roles during fruit ripening, an area not extensively studied in non-model crops. Results Utilizing advanced RNA mass spectrometry techniques, we identified 48 distinct tRNA modifications across 88 sites, alongside 164 genes associated with tRNA modifying enzymes. This comprehensive mapping reveals the decreasing nature of most tRNA modifications during fruit development, except for adenosine 2’-O methylation (Am). It hints at a gradual weakening of protein translation quality control and highlights a unique role for Am during fruit ripening. Additionally, lipidomic analysis tracked 674 lipids in oil palm fruits, indicating a correlation between tRNA modifications and the accumulation of specific lipids. Conclusions This study mapped tRNA modifications in oil palm for the first time and showed the diversity of dynamic changes in tRNA modifications as the fruits develop.

研究领域: 植物学 · 分子生物学 · 农业科学
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JOURNAL OF FOOD SCIENCE · 2017 ·Vol. 82 ·期 12 ·2857-2863 · IF 1.815

Effects of Different Pretreatments to Fresh Fruit on Chemical and Thermal Characteristics of Crude Palm Oil

Minmin Tang,Qiuyu Xia,Brendan J. Holland,Hui Wang,Yufeng Zhang,Rui Li,Hongxing Cao

This study selected 5 methods, including boiling, hot air drying, high-pressurized steam, freezing, and microwave radiation to pretreat fresh oil palm fruit before solvent extraction of the oil. Using fresh fruit as a control, the pretreatment methods were compared for the effects on the activity of the 2 main enzymes in the fruit and some physicochemical properties of the crude palm oil. The results indicated, although all the 5 pretreatments could inactivate lipase and peroxidase in the treated flesh significantly ( P < 0.05), the high-pressurized steam was the most effective. There were also differences in the unsaturated fatty acid contents of the 6 oils. The crude oil from frozen fruit contained significantly more vitamin E (37829.33 ppm) than previously reported. Microwave radiation was shown to significantly decrease the free fatty acid content and the peroxide value, while increasing the oxidative stability index. Thermal behaviors of the oils were significantly different to each other with the exception a few parameters ( P < 0.05).

研究领域: 食品科学 · 生物化学
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ENERGY & FUELS · 2017 · IF 3.091

Production of Liquefied Oil Palm Empty Fruit Bunch Based Polyols via Microwave Heating

Umar Adli Amran,Sarani Zakaria,Chin Hua Chia,Zhen Fang,Mohamad Zulfahdli Masli

Optimization of microwave-assisted liquefaction of oil palm empty fruit bunch fiber (EFB) and cellulose (EFBC) in ethylene glycol (EG) was carried out to produce polyols. The liquefaction residues and hydroxyl numbers of the resultant polyols from respective sources were studied and compared. EFB produced a minimum residue of 3.22% at the optimal parameters of 160 °C and 15 min. Meanwhile, optimum liquefaction of EFBC produced 1.03% residue at 175 °C and 40 min. The maximum hydroxyl numbers of both EFB (749.22 mg KOH/g) and EFBC (639.91 mg KOH/g) polyols were obtained at optimum conditions. FTIR analysis revealed the degradation mechanism of cellulose and lignin in EFB at different temperatures. Lignin was found to be liquefied easily at lower temperatures (130 and 145 °C). However, most of the cellulose began to be liquefied at the optimum temperature (160 °C) and severely degraded at higher temperatures (175 and 190 °C).

研究领域: 生物化学 · 材料科学
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INTERNATIONAL JOURNAL OF ENERGY RESEARCH · 2020 ·Vol. 44 ·期 6 ·4875-4887 · IF 3.741

One-pot synthesis of high N-doped porous carbons derived from a N-rich oil palm biomass residue in low temperature for CO2 capture

Zhixiu Yang,Xiaofei Guo,Guojie Zhang,Ying Xu

Summary Porous carbon materials have been widely used for CO 2 adsorption, but their preparation was subject to conditions such as raw material cost, activator corrosion, and temperature. In this study, nitrogen-doped porous carbonaceous adsorbents were prepared in a low temperature region (400-550°C) by one-step composite nitrogen doping method, using low-cost oil residue as raw materials and less corrosive NaNH 2 as activator. The CO 2 adsorption performances of the prepared N-doped porous adsorbents were systematically explored. The results showed that optimal oil residue-derived carbonaceous adsorbent owned excellent amount of CO 2 adsorption up to 3.51 and 5.63 mmol/g at 298 and 273 K under 1 bar, respectively. It was discovered that the congenerous influences of porous structure, nitrogen content and V n of the adsorbent affected their CO 2 adsorption performances under 1 bar. Importantly, these oil residue porous carbonaceous adsorbent also was verified owning fine selectivity of the CO 2 over N 2 (15.7), which attributed to its high V n and nitrogen content. Furthermore, optimal sample OAC-500-2.5 owned medium Q st (21-26 kJ/mol), which was beneficial practical application. This work may inspire new sparks on novel nitrogen-doped adsorbent with inexpensive precursor, low activation temperature and simple preparative tactic, indicating that the nitrogen-doped sample is promising in the practical situation of CO 2 adsorption.

CAS / MDL: 7782-92-5
研究领域: 材料科学 · 环境科学
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FOOD CHEMISTRY · 2024 · IF 8.5

Influences of superfine-grinding and enzymolysis separately assisted with carboxymethylation and acetylation on the in vitro hypoglycemic and antioxidant activities of oil palm kernel expeller fibre

Bufan Xu,Anyu Zhang,Yajun Zheng,Hui Wang,Xinyu Zheng,Ziqing Jin,Danhong Liu,Nan Wang,Yu Kan

The synergistic effects of ultrafine grinding and enzymolysis (cellulase and Laccase hydrolysis) alone or combined with carboxymethylation or acetylation on the hypoglycemic and antioxidant activities of oil palm kernel fibre (OPKEF) were studied for the first time. After these synergistic modifications, the microstructure of OPKEF became more porous, and its soluble fibre and total polyphenols contents, and surface area were all improved ( P  < 0.05). Superfine-grinding and enzymolysis combined with carboxymethylation treated OPKEF exhibited the highest viscosity (13.9 mPa∙s), inhibition ability to glucose diffusion (38.18%), and water-expansion volume (3.58 mL∙g −1 ). OPKEF treated with superfine-grinding and enzymolysis combined with acetylation showed the highest surface hydrophobicity (50.93) and glucose adsorption capacity (4.53 μmol∙g −1 ), but a lower α-amylase-inhibition ability. Moreover, OPKEF modified by superfine-grinding and enzymolysis had the highest inhibiting activity against α-amylase (25.78%). Additionally, superfine-grinding and enzymolysis combined with carboxymethylation or acetylation both improved the content and antioxidant activity of OPEKF's bounding polyphenols ( P  < 0.05).

研究领域: 食品科学 · 生物化学
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PROTEIN EXPRESSION AND PURIFICATION · 2020 · IF 1.513

Comparison of three palm tree peroxidases expressed by Escherichia coli: Uniqueness of African oil palm peroxidase

Meile Yuan,Hongwei Zhao,Qian Huang,Xianhua Liu,Yanyu Zhou,Xiaoping Diao,Qing X. Li

Palm tree peroxidase has greater catalytic activity, stability and broad application prospects in comparison with horseradish peroxidase . However, slow growth, ecological destruction and high costs prohibit isolation of native peroxidases directly from palm trees. Bioreactor production of palm tree peroxidases would therefore be preferred to overcome such production limitations. Comparison of different recombinant glycan-free palm tree peroxidases would allow understanding the criticality of total glycans to the functions and characteristics. In the present study, African oil palm tree peroxidase expressed by Escherichia coli showed similar stability and 30-100-fold greater activity than that of recombinant royal palm tree peroxidases, but both of their comprehensive indexes were superior to the commercial, native horseradish peroxidase. Recombinant Chamaerops excelsa peroxidase showed no activity possibly due to incorrect protein folding . The results confirmed that recombinant expression by E. coli is potentially an effective means to obtain a mass of palm peroxidases with high activity and stability.

研究领域: 生物化学 · 生物技术 · 植物科学
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