Five fluorescent dyes – rhodamine B (RB), rhodamine 6G (R6G), lissamine rhodamine B (LRB), 3, 6-diamincacridine hulmisulfate (DH) and safranine T (ST) – were used to label seeds as an anti-counterfeiting technology. The effects of the d yes on physiological parameters of
rape and the patterns of fluorescence of rape seedlings were investigated. The results showed that s oaking seeds for 24 hours in 0.1 mg RB ml−1, 0.1 mg LRB ml−1 or 0.1 mg ST ml−1 had no significant effects on seed germination, seedling growth,
seedling protective enzyme activities or chlorophyll content. RB and LRB emitted bright red and bright orange fluorescence under a fluorescence microscope, when excited by green light (546 nm) and blue light (495 nm), respectively, whereas ST showed little difference compared with the control.
Moreover, the vascular bundles of stem, seedling roots and aerial parts of seedlings treated with RB all emitted brilliant fluorescence for a long time, which could be used as a marker in seedlings. Our results suggest that labelling rape seeds with RB dye at a suitable concentration can be
used as an anti-counterfeiting technique.
Seed oil content and fatty acid (FA) composition collectively determine the quality and economic value ofBrassica napus. Little is known about the role of R2R3-MYB transcription factors (TFs) in regulating FA biosynthesis inB. napus. Here,BnaC04.MYB89was found to be expressed primarily in developing seeds. Overexpression ofBnaC04.MYB89consistently decreased FA levels, as evidenced by its effect in both theArabidopsis thaliana myb89-1mutant andB. napusseeds. RNA-seq of developing seeds at 30 DAP (days after pollination) revealed marked suppression of FA biosynthetic genes inBnaC04.MYB89-overexpressing plants compared to the K407 control. ChIP (Chromatin immunoprecipitation) analysis revealed that BnaC04.MYB89 directly inhibited the expression ofBnaA03.BCCP1andBnaC03.HDwhile indirectly regulating that ofBnaA09.BADC1,BnaA03.BADC3,BnaA03.MOD1, andBnaA08.FAT8, thereby reducing seed FA accumulation. Collectively, these results elucidate the role for BnaC04.MYB89 and provide new insights into the transcriptional regulatory network controlling seed oil accumulation inB. napus.
Xuezhi Wang,Yuan Sun,Ming Liu,Guyu You,Ting Fan,Hongmiao Wu,Liwei Xu,Xiaoli Zhao,Dongjie Ma
Polylactic acid (PLA) plastic is increasingly recognized as a sustainable alternative to petroleum-based plastics. However, its biodegradability remains a challenge, requiring substantial improvements to its biodegradation efficiency. This study explored the use of combined hydrothermal treatment (HTT) (160°C–240°C) and anaerobic digestion (AD) to improve methane yield from PLA. HTT enhanced significant structural changes in PLA, yielding 2-hydroxypropanoic acid, 1-decanol, 1-tetradecanol, pentan-2-one, and butyl 2-hydroxypropanoate, which facilitated PLA degradation. The methane yield from AD of hydrothermal-treated PLA (HTT-PLA) products at 180°C was 493 NmL/g-volatile solid, a 21.4-fold increased compared to untreated PLA. Genomic and proteomic analyses identified key genes and proteins involved in HTT-PLA products biodegradation, elucidating pathway for HTT-PLA products transformation in biodegradation process. The residues from AD of HTT-PLA products at 180°C exhibited rapeseeds germination index of approximately 90% and maintain stability locomotion and vitality in Caenorhabditis elegans , indicating their suitability for land application. Enhanced methane production offers a renewable energy source, and reduces PLA’s environmental footprint, providing significant practical implications for bioplastic waste management and sustainability.
危险品化学品经营许可证（带存储）