JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
·Vol. 104
·期 11
·6787-6798
·
IF 3.3
Xinyuan Huang,Xin Li,Yufeng Zhang,Xiefei Li,Pei Zhang,Hongbo Song,Qun Huang,Guiming Fu
BACKGROUND Gel property is among the crucial functional properties of egg yolk (EY), which determines the texture and flavor of EY products. In the present study, the effects of two unsaturated fatty acids [monounsaturated fatty acid oleic acid (OA) and diunsaturated fatty acid linoleic acid (LA)] on the gel properties of EY protein were investigated. RESULTS Compared with the blank group, the addition of LA and OA (10–50 g kg −1 ) improved the gel hardness (from 270.54 g to 385.85 g and 414.38 g, respectively) and viscosity coefficient (from 0.015 Pa.s n to 11.892 Pa.s n and 1.812 Pa.s n , respectively). The surface hydrophobicity of EY protein increased to a maximum value of 40 g kg −1 with the addition of both fatty acids (39.06 μg and 41.58 μg, respectively). However, excess unsaturated fatty acids (≥ 50 g kg −1 ) disrupted the completeness of the gel matrix and weakened the structural properties of the EY gel. CONCLUSION Both fatty acids improved the gel properties of EY protein. At the same addition level, OA was superior to LA in improving gel properties. The present study provides a theoretical underpinning for the sensible application of unsaturated fatty acids in improving EY gel properties. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Catalysts
·
2021
·Vol. 11
·期 12
·1471
·
IF 4.146
Zhenzhen Li,Wei Liu,Guolong Yang
Synthesized oleic acid-based wax esters (e.g., cetyl oleate), which can replace spermaceti oil or jojoba oil, have been widely used in the cosmetic, pharmaceutical and other industries. In this work, 4-dodecylbenzenesulfonic acid (DBSA) has been successfully used as an efficient catalyst to synthesize oleic acid-based wax esters through esterification at 40 °C under solvent-free conditions. A 93.6% conversion rate of cetyl alcohol was obtained under optimal conditions: 10 mol% DBSA, a molar ratio of 1.3:1 oleic acid to alcohol, a reaction temperature 40 °C and a reaction time of 4 h. The effect of water content on esterification was investigated, and it was found that the inhibitory effect of water decreased significantly with increasing temperature. Moreover, DBSA-catalyzed esterification could be applied in the production of various oleic acid-based wax esters and excellent conversion (>90%) to esters was obtained under such mild conditions. DBSA-catalyzed low-temperature esterification is an efficient method for the production of liquid wax esters.Keywords:liquid wax esters;4-dodecylbenzenesulfonic acid;esterification;oleic acid;high-oleic sunflower seed oil
研究领域:
化学工程
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化妆品科学
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生物催化
✓ 已验证
产品关联
DOI
高影响因子
Bioanalysis
·
2015
·Vol. 7
·期 9
·
IF 3.003
Shirui Zheng,Zhiyuan Ma,Feifeng Song,Jianfeng Ye,Ruwei Wang,Meijuan Tu,Liping Li,Hui Zhou,Su Zeng,Huidi Jiang
Background: Deficiency or imbalance of unsaturated fatty acids will promote the pathogenesis of many diseases. In order to monitor the exposure of unsaturated fatty acids, the method based on LC–MS/MS was developed. Results: Standard calibration curves for α-linolenic acid, linoleic acid, palmitoleic acid and oleic acid were linear (r ≥0.99). The intra-and interbatch accuracy (RE%) ranged from -4.5 to 8.6%, while the intra- and interbatch precisions (RSD%) were ≤8.7%. The extraction recovery varied from 85.4 to 99.6%, and no obvious matrix effect was observed. Conclusion: The method offers a simple approach for measuring 4 unsaturated fatty acids in 1 μl rat plasma within 3.95 min.
研究领域:
分析化学
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医学
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营养学
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DOI
PubMed
高影响因子
Frontiers in Oncology
·
2025
·
IF 3.3
Xiaocui Zhong,Mamona Bilal,Yanqiu Zhou,Xiaojia Yang,Zuchao Qin,Qibing Li,Yang Yang,Ting-Li Han,Min Li
Introduction Aberrant fatty acid (FA) metabolism is increasingly recognized as a significant factor in ovarian cancer (OC) progression, although the comprehensive metabolic alterations across different body tissues remain unclear. Methods In this study, sixteen OC patients and twenty-nine non-cancer (NC) patients were recruited for metabolic profiling using a global and targeted metabolomic strategy based on a gas chromatography-hydrogen flame ionization detector (GC-FID). The patient survival was followed up to 3 years, and PFS was calculated. Results Our findings revealed distinct metabolite profiles that differentiate OC from NC groups across all sample types. We found seven, nine, and thirteen significant metabolites in subcutaneous fat, plasma, and ovarian tissue respectively. In particular, docosahexaenoic acid (DHA) and arachidonic acid (AA) levels were notably elevated in all sample types of OC patients. Furthermore, receiver operating characteristic (ROC) analysis highlight that three plasma FA showed the best specificity and sensitivity in differentiating the OC group from the NC group (Area Under The Curve, AUC > 0.89), including caprylic acid, myristoleic acid, and tetracosaenoic acid. Most of the significant FA in subcutaneous fat and ovarian tissue showed a high risk of OC. However, caprylic acid and tetracosanoic acid were identified as protective factors in the plasma sample. We also found that high levels of linoelaidic acid in subcutaneous fat and palmitelaidic acid in ovarian tissue were associated with poor prognosis. Pathway analysis indicated upregulation of fatty acid synthesis, inflammatory signaling, and ferroptosis pathways in OC patients. Discussion This study reveals a coordinated reprogramming of FA metabolism across multiple biospecimens in OC patients. Our results suggest that specific fatty acids may contribute to OC progression through dysregulation of fatty acid synthesis, inflammatory signaling, and ferroptosis. These findings offer mechanistic insights into OC progression and highlighting potential biomarkers and targeted therapeutic interventions.
研究领域:
肿瘤学
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代谢组学
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生物化学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Panpan Xu,Fengdan Yan,Mengyang Liu,Yanlan Bi,Shangde Sun,Jun Li
Effects of N-methylimidazole hydrogen sulfate, 2-pyrrolidone hydrogen sulfate, N-methyl pyrrolidone hydrogen sulfate, and their -SO 3 H functionalized acidic ionic liquids were comparatively investigated on the dehydration degree of ricinoleic acid (RA) sample and conjugated linoleic acid (CLA) content in the resulting dehydrated ricinoleic acid (DRA) sample. Results showed the -SO 3 H functionalized N-methyl pyrrolidone hydrogen sulfate ([C 3 SO 3 Hnmp]HSO 4 ) had the strongest catalytic activity among the six ionic liquids. Under the optimal conditions (4 % [C 3 SO 3 Hnmp]HSO 4 , 250 °C, and 30 min), the dehydration degree of RA sample reached 98.69 % with 42.57 % of CLA in the resulting DRA sample. Results from nuclear magnetic resonance, Fourier transform infrared spectroscopy, and mass spectrometry indicated the hydroxyl group of RA underwent intramolecular dehydration to form new double bonds; RA sample was randomly dehydrated with both non-conjugated linoleic acid and CLA present in DRA sample. Rheological analysis showed that RA sample had a higher viscosity than DRA sample due to the presence of hydrogen bonds. Thermogravimetric analysis showed RA and DRA samples were thermally stable at up to 250 °C.
研究领域:
食品科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Niu Zhang,Jun-Peng Zeng,Yan-Ping Wu,Meng Wei,Hong Zhang,Liufeng Zheng,Ze-Yuan Deng,Jing Li
The digestion and absorption of different structural lipids in human milk may be different. Hence, by simulating in vitro infant digestion and Caco-2 cells to explore the effects of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL)/1,3-dilinoleoyl-2-palmitoylglycerol (LPL)/1,3-dioleoyl-2-palmitoylglycerol (OPO) and their mixtures (M) (OPL/LPL/OPO in M1, M2, and M3 were 1.5/0.5/1, 1.2/1.2/1, and 0.5/0.2/1, respectively) on digestion and absorption. The digestibility of the OPO group was higher than those of the OPL and LPL groups, and the M3 group was higher than the M1 and M2 groups. The synthesis and transport of triglycerides in Caco-2 cells in OPL and LPL groups were higher than the OPO group, and the M1 group was significantly higher than that of M3. The expression of FABP1, PPARα, and MTT protein in OPL and M1 groups was significantly higher than OPO and M3, respectively. There are differences in the digestion and absorption of differently structured lipids from this study.
研究领域:
营养学
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生物化学
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儿科
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产品关联
DOI
PubMed
高影响因子
POLYMER
·
2024
·
IF 4.1
Minghao Wang,Haomin Yu,Jiawei Jiang,Wencong Xu,Guofeng Xu,Si Wu
Cis-trans isomerism plays a crucial role in regulating the thermal properties and phase transitions of materials, which is essential for the development of advanced functional materials. By influencing melting points and glass transition temperatures, cis-trans isomerism enables precise control over material performance in applications such as thermal sensors, self-healing coatings, thermal sensors, and temperature-responsive films. Here we report the impact of cis-trans isomerism in double bonds on the thermal and phase transitions of oleic acid (OA) and elaidic acid (EA) and their derived polymers, with a focus on glass transition temperature ( T g ) and melting point ( T m ). A series of molecules and copolymers with varying cis and trans double bond ratios are synthesized to examine how these isomeric configurations affect thermal behavior. Thermal and structural properties are characterized using differential scanning calorimetry (DSC) and nuclear magnetic resonance (NMR) spectroscopy. The results show a clear correlation between isomeric composition and the polymers' T g and T m , illustrating that the incorporation of cis-trans isomers can be effectively used to fine-tune thermal properties. This research offers valuable insights into the design of advanced polymeric materials with tailored thermal characteristics, paving the way for applications in various high-performance sectors.
研究领域:
材料科学
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化学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
·
IF 5.2
Yucai Lu,Jiuyu Chen,Baozhong Zhu,Jiquan Wang,Cong Jiang,Minggao Xu,Yunlan Sun
Aluminum-containing hydrocarbon fuels cannot fully release their energy due to the poor dispersion and easy agglomeration of aluminum nanoparticles (ANPs). To solve the issues, oleic acid (OA) was used to modify the surface of ANPs, and then Al@OA particles were incorporated into exo-tetrahydrodicyclopentadiene (JP-10) to form Al/JP-10 bi-phase system. Experimental and molecular simulation methods were used to analyze the stability, ignition, and combustion performance of the Al/JP-10 bi-phase system. The results show that at low temperatures, OA can improve the antioxidant and hydrophobic performance of Al@OA particles, which can effectively protect the ANPs from deep oxidation . At high temperatures, OA can accelerate the oxidative exothermic process of ANPs, and the Al@OA particles can shorten the ignition delay time of JP-10 droplets and intensify the combustion. Notably, when the ratio of Al and OA is 10:3 in the Al@OA particles, the Al/JP-10 droplet exhibits the shortest ignition delay.
研究领域:
材料科学
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燃料化学
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分子动力学
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产品关联
DOI
高影响因子
Journal of Biobased Materials and Bioenergy
·
2012
·Vol. 6
·期 2
·178-184
·
IF 1.037
Li Xian-Fa,Fu Yao
A solid acid catalyst KLC-SO3H bearing SO3H, COOH and phenolic OH groups was synthesized by the sulfonation of industrial waste kraft kraft lignin-based activated carbon (KLC) which was prepared from phosphoric acid (PA) activation. The KLC support and the catalyst
were characterized by a variety of techniques. The prepared catalyst exhibits good activity to catalyze the esterification of oleic acid with methanol. Yield of methyl oleate as high as 93.2% was obtained under the temperature of 80 °C, methanol to oleic acid molar ratio of 10:1, reaction
time of 12 h and catalyst loading of 10% (based on oleic acid). The prepared KLC-SO3H catalyst showed similar catalytic activity with the carbon acid catalyst from cellulose and the commercial catalyst Amberlyst-15 in the esterification reaction. The high catalytic activity of KLC-SO3H
was probably due to the large specific surface area, pore volume of KLC support, which provided more acid sites for the reactants.
研究领域:
化学工程
·
催化材料
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2024
·Vol. 99
·期 12
·2693-2702
·
IF 2.8
Yandong Lu,Jiaxu Ning,Yue Zong,Fu Ding,Gaige Liu,Ruijun Zhang
BACKGROUND Tailored design of high-performance nanofiltration membranes is desirable. The physicochemical properties of the organic phaseare essential for the preparation of nanofiltration membranes. Therefore, byfine-tuning the organic phase, it is possible to achieve nanofiltrationmembranes with enhanced performance. RESULTS In this work, oleic acid, a naturally extracted fatty acid, wasintroduced into three different organic solvents (n-heptane, n-octane andisopar G) to construct special oil phases for interfacial polymerization. Testingresults showed that proper dosage of oleic acid addition can effectivelydecrease the nanofiltration membrane pore size and enhance the salt rejectionregardless of the solvent type. Taking n-octane as example, 5% oleic acidaddition in it can decrease the mean pore size from 0.500 nm to 0.341 nm and improve the MgSO 4 rejection from 52.4% to 95.5%. Further characterizationindicated that the introduction of oleic acid into organic solvent cansignificantly reduce the interfacial tension and promote the diffusion of piperazinefrom aqueous phase to the oil phase. In addition, oleic acid could react with piperazineand produce some white particles that could be embed into the polyamide layer, thus altering the surface morphology. CONCLUSION In this work, a novel modified thin film composite nanofiltration membrane was prepared by a simple and controllable oleic acid assisted interfacial polymerization strategy, which exhibits good water permeability, solute selectivity and antifouling property. Without changing the existing process, our strategy opens up a simple, environmentally friendly and operable route for the synthesis of thin film composite nanofiltration membranes. © 2024 Society of Chemical Industry (SCI).
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOLOGICAL CHEMISTRY
·
2020
·Vol. 295
·期 19
·6425-6446
·
IF 4.238
Chengmeng Jin,Xiaobing Zhu,Hao Wu,Yuqi Wang,Xun Hu
Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the PGK1-catalyzed forward reaction. The Km values for 1,3-bisphosphoglyceric acid (1,3-BPG, the forward reaction substrate) were 4.36 μm (yeast PGK1) and 6.86 μm (human PKG1). The Km values for 3-phosphoglycerate (3-PG, the reverse reaction substrate and a serine precursor) were 146 μm (yeast PGK1) and 186 μm (human PGK1). The Vmax of the forward reaction was about 3.5- and 5.8-fold higher than that of the reverse reaction for the human and yeast enzymes, respectively. Consistently, the intracellular steady-state concentrations of 3-PG were between 180 and 550 μm in cancer cells, providing a basis for glycolysis to shuttle 3-PG to the serine synthesis pathway. Using siRNA-mediated PGK1-specific knockdown in five cancer cell lines derived from different tissues, along with titration of PGK1 in a cell-free glycolysis system, we found that the perturbation of PGK1 had no effect or only marginal effects on the glucose consumption and lactate generation. The PGK1 knockdown increased the concentrations of fructose 1,6-bisphosphate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, and 1,3-BPG in nearly equal proportions, controlled by the kinetic and thermodynamic states of glycolysis. We conclude that perturbation of PGK1 in cancer cells insignificantly affects the conversion of glucose to lactate in glycolysis.
研究领域:
生物化学
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癌症生物学
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细胞代谢
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 1
·108-117
·
IF 4.125
Chuanqing Huang,Zepeng Lin,Yunlong Zhang,Zeyu Liu,Xiaoyue Tang,Changzhu Li,Lin Lin,Wenqian Huang,Yong Ye
Background Diacylglycerol (DAG)-enriched oil has been attracting attention because of its nutritional benefits and biological functions, although the composition of its various free fatty acids (FFAs) and an unclear relationship between substrate and yield make it difficult to be identified and qualified with respect to its production. In the present study, linoleic acid-enriched diacylglycerol (LA-DAG) was synthesized and enriched from Camellia oil by the esterification process using the combi-lipase Lipozyme TL IM/RM IM system. Results The relationship between FFA composition and DAG species productivity was revealed. The results showed that heterogeneous FFA with a major constituent (more than 50%) exhibited higher DAG productivity and inhibited triacylglycerol productivity compared to homogeneous constituents. Joint characterization by high-performance liquid chromatography-evaporative light scattering detection, gas chromatography-mass spectrometry and ultra-performance liquid chromatography-heated electrospray ionization-tandem mass spectrometry identified that DAG components contained dilinoleic acid acyl glyceride, linoleyl-oleyl glyceride and dioleic acid acyl glyceride in esterification products. Under the optimum conditions, 60.4% 1,3-DAG and 61.3% LA-DAG in the crude product at 1 h reaction were obtained, and further purified to 81.7% LA-DAG and 94.7% DAG via silica column chromatography. Conclusion The present study provides a guideline for the identification of DAG species, as well as a structure-guided preparation method of DAG-enriched oils via the cost-effective combi-lipase. © 2022 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）