CELL
·
2025
·
IF 42.5
Nan Zhou,Jingzhi Chen,Meiqin Hu,Na Wen,Weijie Cai,Ping Li,Liding Zhao,Yaping Meng,Dongdong Zhao,Xiaotong Yang,Siyu Liu,Fangqian Huang,Cheng Zhao,Xinghua Feng,Zikai Jiang,Enjun Xie,Hongxu Pan,Zhidong Cen,Xinhui Chen,Wei Luo,Beisha Tang,Junxia Min,Fudi Wang,Junsheng Yang,Haoxing Xu
Lysosomes maintain an acidic pH of 4.5–5.0, optimal for macromolecular degradation. Whereas proton influx is produced by a V-type H + ATPase, proton efflux is mediated by a fast H + leak through TMEM175 channels, as well as an unidentified slow pathway. A candidate screen on an orphan lysosome membrane protein (OLMP) library enabled us to discover that SLC7A11, the protein target of the ferroptosis-inducing compound erastin, mediates a slow lysosomal H + leak through downward flux of cystine and glutamate, two H + equivalents with uniquely large but opposite concentration gradients across lysosomal membranes. SLC7A11 deficiency or inhibition caused lysosomal over-acidification, reduced degradation, accumulation of storage materials, and ferroptosis, as well as facilitated α-synuclein aggregation in neurons. Correction of abnormal lysosomal acidity restored lysosome homeostasis and prevented ferroptosis. These studies have revealed an unconventional H + transport conduit that is integral to lysosomal flux of protonatable metabolites to regulate lysosome function, ferroptosis, and Parkinson’s disease (PD) pathology.
研究领域:
细胞生物学
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医学
·
神经科学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2019
·Vol. 126
·期 1
·102-112
·
IF 2.683
Lin W.,Yuan D.,Deng Z.,Niu B.,Chen Q.
ObjectivesIn our previous research, we have developed a new combination disinfectant, glutaraldehyde‐didecyldimethylammonium bromide (GD). It was verified that GD had a strong effect on both Escherichia coli and Staphylococcus aureus. In this work, Candida albicans was selected as an object, and it could be killed by GD. We aimed to investigate the cellular and molecular mechanism of GD effecting on C. albicans.Methods and ResultsThe results of sterilization experiment indicated that GD was effective on C. albicans. Flow cytometry and atomic absorption spectrometry were applied to detect cell membrane damage of C. albicans. Luciferase reaction and Bradford method were carried out to detect ATP content and protein quantitation. Transmission electron microscopy was used for intracellular organelles morphological observation. In order to study changes in mitochondrial membrane potential, Rh 123 was used as an indicator. DNA conformation analysis was performed by molecular modelling and circular dichroism. The results indicated that membrane permeability was increased rapidly owing to GD effect, and the leaked K+ and Mg2+ were about 12·1 and 12·4 times those of the control, respectively, at 10 min after GD treatment. Simultaneously, ATP and protein also leaked rapidly out of the cell. Mitochondrial membrane potential was destroyed, succinic dehydrogenase activity was significantly decreased and DNA conformation was changed because of GD action.ConclusionsGlutaraldehyde‐didecyldimethylammonium bromide disinfected C. albicans through distorting cell membrane integrity and permeability, disturbing the intracellular homeostasis by intracellular substances leakage, especially K+, Mg2+, ATP and protein, causing electrolyte imbalance of mitochondria, changing DNA structure, which finally led to cell death.Significance and Impact of the StudyThis study focused on the cellular and molecular mechanism of GD as a disinfectant against C. albicans. It is important to provide theoretical support to GD against Candida albicans in practical application.
研究领域:
微生物学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
Microorganisms
·
2024
·Vol. 12
·期 9
·1871
·
IF 4.1
Lankun Yi,Mingze Cao,Xu Chen,Yubin Bai,Weiwei Wang,Xiaojuan Wei,Yuxiang Shi,Yongying Zhang,Tenghe Ma,Zhen Zhu,Jiyu Zhang
Bacterial drug resistance is becoming an increasingly serious problem, and the development of antibacterial synergists is urgently needed. Combining existing antibiotics with promising nonantibiotic agents is one strategy that has been shown to be effective at overcoming the widespread emergence of antibiotic-resistant pathogens. In this study, we investigated the antibacterial activities and mechanism of naringenin (NG) combined with amikacin (AMK) against multidrug-resistantEscherichia coli(E. coli). We first measured the fractional inhibitory concentration (FIC) of NG combined with antibiotics via the checkerboard method. The results indicated that the combination of NG and AMK had a synergistic effect onE. coliATCC 25922 andE. coliC7F3. In addition, this synergistic effect was verified by time-kill assays. Moreover, scanning electron microscopy (SEM) was used to observe cell morphology. The results showed that the cell wall ofE. coliwas destroyed. Furthermore, we assessed the leakage of alkaline phosphatase (AKP), K+, and protein. The extracellular AKP activity increased after the combinational group of 1/2MIC NG and 1/2MIC AMK, suggesting an impairment in cell wall permeability. An increase in the leakage of intracellular K+and protein indicated an increase in cell inner membrane permeability. These results revealed that NG and AMK inhibitedE. coliby damaging cell walls and membranes. In addition, PI uptake rapidly increased after treatment with NG and AMK. Confocal laser scanning microscopy (CLSM) revealed that NG caused cell wall and cell membrane damage inE. coli. In summary, our results provide a new strategy for responding to the development ofE. colidrug resistance.
研究领域:
微生物学
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药理学
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抗菌研究
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DOI
PubMed
高影响因子
MACROMOLECULES
·
2025
·
IF 5.2
Yuan Yuan,Yu Jiang,Zhihao Huang,Zhiguang Xu,Tao Zhang,Yan Zhao
Phase change material (PCM)-microencapsulated monolithic composites hold considerable promise in applications for latent heat storage and release, but they face the issue that the microencapsulated PCMs, when in the liquid state, might leak via an open pathway. Herein, we report the synthesis of solid–solid phase change composites from thermoreversible octadecane (OD) gel-in-water emulsions through in situ closed-cell polyHIPE generation and encapsulation. The thermoreversible OD gels, formed simply by heating OD and a low-molecular-weight gelator together, exhibit a reversible crystal-gel phase transition and a gel–liquid phase transition. Through light-driven interface-initiated radical polymerization, solid–solid phase change composites have been fabricated from Pickering emulsions containing the thermoreversible OD gel as the dispersed phase via in situ closed-cell polyHIPE generation and encapsulation. The resulting composites show predesignable external shapes, negligible leakage even for fractured samples after 10 heating–cooling cycles, high flexibility, and robust compression. The composites possess extremely high heats of melting and crystallization (up to 197.5 J/g) and good reusability. The advantageous properties enable the solid–solid composites to be excellent candidates for efficient latent heat storage and temperature management.
研究领域:
材料科学
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能源工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Shichong Guo,Limei Dong,Junfeng Li,Zhenzong Lu,Yanshuo Li,Mutai Bao,Yiming Li
Crude oil spills in marine ecosystems have been a global environmental problem. Photothermal oil-adsorbing materials have shown enormous potential in cleaning up viscous crude oil spills, yet challenging to achieve efficient adsorption under insufficient light. Herein, a simple Pickering emulsion route was used to encapsulate phase change materials (PCMs) in photothermal cellulose-based aerogels (Pa@SCCA) for efficiently utilizing solar energy and remediating viscous crude oil spills. Pa@SCCA demonstrates excellent shape stability, thermal stability and importantly it shows outstanding hydrophobicity and compressibility due to methyltrimethoxysilane (MTMS) modification, thus enabling it to selectively adsorb and recover viscous crude oil. Carbon nanotubes (CNTs) was doped in the Pa@SCCA to allow the efficient conversion from solar energy to heat, and PCMs with thermal-storage ability can continuously reduce the viscosity of crude oil even under insufficient light by releasing thermal energy. Significantly, the preheated Pa@SCCA can remove viscous crude oil spills from cryogenic seawater (10 ℃) without light assistance. The introduction of PCMs improves overall solar energy utilization efficiency and the adsorption capacity for viscous crude oil under unstable light. The coupling of photothermal porous materials with PCMs offers significant advantages over conventional photothermal adsorbents.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Lingfang Xu,Yu Zhang,Chi Gui,Hanshuo Fang,Ruilong Wang,Haibo Xiao,Shiheng Liang,Changping Yang
BiFeO 3 has been extensively studied as an important single-phase multiferroic material at room temperature in the application fields of energy storage, data storage, spintronics, and electro-magnetic sensors. However, the high leakage current caused by the presence of lattice defects such as vacancies constrains the further development of BiFeO 3 . In this paper, we presented a facile method to overcome the high leakage problem via blending BiFeO 3 nanoparticles into the ferroelectric copolymer PVDF-TrFE matrix and introducing a barrier layer beneath the top Pt dot electrode. The leakage current and ferroelectric characteristics of BiFeO 3 _PVDF-TrFE nanocomposite films were discussed comprehensively. Consequently, compared with the composites without the barrier layer, the leakage current density of the BiFeO 3 _PVDF-TrFE composite films reduced two orders of magnitude after coating a pure copolymer layer. Moreover, the remnant ferroelectric polarization reached ~14 μC cm −2 for a moderate weight content (25 wt%) of BiFeO 3 nanoparticle in the blends, with a remarkably smaller coercive electric field than that of pure PVDF-TrFE films. An electrical conduction blocking mechanism was established to elucidate the optimized electric and ferroelectric properties of nanocomposite films.
研究领域:
材料科学
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电子工程
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Jinli Zhao,Juan Li,Qingbai Chen,Mengwei Yang,Yongkang Li,Hong Li,Yuzhong Zhang,Jianyou Wang
The influence of crystallization behavior on membrane structure and performance has been extensively studied in many membrane fields. Some studies of proton exchange membranes used in fuel cells have involved the influence of crystallization. However, crystallization of ion exchange membranes (IEMs) used in electrodialysis (ED) has been rarely studied. Co-ion leakage in ED process is adverse, but there have been few mechanism studies regarding how co-ions cross IEM surfaces on the concentration chamber side and migrate into the dilution chamber to reduce current efficiency. Polyvinylidene fluoride is a kind of good crystalline material. In this study, sodium dodecyl benzene sulfonate was introduced as the functional material , with polyvinylpyrrolidone (PVP) as the hydrophilizing agent. They were blended to prepare cation exchange membranes (CEMs). Amorphous PVP was used to regulate membrane crystallization behavior. The results showed that, when the ion exchange capacity is basically equal, surface roughness of the prepared crystalline CEMs was low and the structure dense. The ED efficiency significantly surpassed amorphous CEMs. The co-ion leakage of amorphous CEMs was greater than those of crystalline CEMs due to the rough and non-dense surfaces of amorphous CEMs. However, after densification modification, although surface resistance increased, co-ion leakage was effectively suppressed. Combining simulation and theoretical analysis, it was presumed that dense membrane surfaces on the concentrated chamber side was very important for suppressing co-ion leakage. This study on the influence and mechanism of crystallinity and co-ion leakage was helpful for optimizing CEM mass transfer and improving current efficiency.
研究领域:
材料科学
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环境工程
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产品关联
DOI
高影响因子
STAR Protocols
·
2024
·
IF 1.3
Zengguang Sui,Yunren Sui,Zhixiong Ding,Haosheng Lin,Fuxiang Li,Wei Wu
Summary Passive thermal management with zero-energy consumption and high compactness has drawn increasing attention. Here, we present a protocol to develop a hygroscopic salt-loaded heat sink with a moisture-permeable membrane encapsulation technique for electronics cooling. We describe steps for preparing lithium bromide solution and heat sink with anti-corrosion graphene coating. We then detail procedures for preparing the hygroscopic salt-loaded membrane-encapsulated heat sinks (HSMHSs). The produced low-cost HSMHS exhibits remarkably high thermal management performance without the risks of leakage and corrosion. For complete details on the use and execution of this protocol, please refer to Sui et al. 1
研究领域:
材料科学
·
热能工程
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·Vol. 12
·期 28
·2301379
·
IF 10
Qijiang Mao,Zhengze Huang,Yiyin Zhang,Qi Chen,Kexin Jiang,Yi Hong,Hongwei Ouyang,Yuelong Liang
Colon leakage is one of the most severe complications in abdominal trauma or surgery cases. It can lead to severe abdominal infection and abdominal adhesions, resulting in prolonged hospital stays and increased mortality. In this study, a photosensitive hydrogel is proposed, which can swiftly form a strong adhesion coating on the damaged colon after UV irradiation, to realize quick cure and suture-free repair of colon leakage. The newly developed biological gel consists of hyaluronic acid methacryloyl (HAMA) and hyaluronic acid o-nitroso benzaldehyde (HANB) in the optimal ratio of 3: 1, which exerts both the rapid photocuring properties of HAMA and the strong tissue adhesion properties of HANB. HAMA/HANB shows excellent adhesion stability on wet surfaces, presenting controllable mechanical properties, ductility, adhesion stability, and chemical stability; it also evades foreign body response, which relieves the degree of abdominal adhesion. The underlying mechanism for HAMA/HANB promoting wound healing in colon leakage involves the reconstruction of the colon barrier, as well as the regulation of the immune reaction and neovascularization. In all, HAMA/HANB is a promising alternative suture-free approach for repairing colon leakage; it has a reliable healing effect and is expected to be extended to clinical application for other organ injuries.
研究领域:
医学
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生物材料学
·
外科手术
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DOI
高影响因子
COMPOSITES SCIENCE AND TECHNOLOGY
·
2024
·
IF 8.3
Siyuan Qiu,Yajiao Li,Yi An,Wenhao Wang,Yuanmin Chen,Ke Chen,Daming Wu,Jingyao Sun
Phase change composites (PCCs) have attracted much attention in the fields of thermal management due to their high latent heat. However, their risk of leakage and poor shape designability greatly limit their industrial applications. Therefore, there is an urgent need to develop leakage-proof and customizable PCCs to meet the emerging requirements of thermal management applications. Some scholars have proposed the concept of preparing PCCs by 3D printing technology, aiming to meet customized thermal management requirements of various electronic devices. Nevertheless, the phase change material leaking of PCCs under high temperature is still a tough problem to solve. In this study, expanded graphite (EG) is used as the carrier for paraffin wax (PW), which names as EP can tightly enveloping PW in its porous structure. Then, an innovative carbomer gel ink is prepared for 3D printing using EP and short carbon fiber (SCF) as thermal conductive fillers. Freeze-drying and polydimethylsiloxane (PDMS) infiltrating procedures are furtherly performed to ensure the flexibility of final PCCs samples. A maximum thermal conductivity of 2.89 W/(m·K) is obtained when the content of SCF/EP filler is 10 wt%. Importantly, the flexible PCCs prepared through this method effectively prevent the PW leaking during thermal management applications, thereby avoiding the consequent safety risks and enhancing the lifespan of electronic devices. This work opens up a promising pathway for the rapid fabrication of leakage-proof, customizable and flexible PCCs.
研究领域:
材料科学
·
热能工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2018
·Vol. 135
·期 18
·46222
·
IF 1.901
Xia Gao,Tianbo Zhao,Guan Luo,Baohui Zheng,Hui Huang,Rui Ma,Xue Han,Yuqiao Chai
Polystyrene (PS) microencapsulated paraffin wax (MePW) and PS–SiO 2 MePW were used to improve the form-stability of PW in hydroxyl-terminated polybutadiene-derived polyurethane (HTPB) binder. HTPB matrix containing different contents of PS MePW, PS–SiO 2 MePW, and PW were prepared. The chemical composition, crystallinity, microstructure, heat capacities, thermal stabilities, thermal reliabilities, leakage, and mechanical properties of the composites were compared using Fourier transforms infrared spectroscope, X-ray diffractometer, scanning electronic microscope, differential scanning calorimeter, thermo-gravimetric analyzer, thermal cycling test, leaking test, compression, and tensile tests, respectively. The results showed that the MePW/PW/HTPB composites were prepared without chemical reaction. The thermal stability and mechanical properties of PS–SiO 2 MePW/PW/HTPB increased more dramatically than that of PS MePW/PW/HTPB. With the increasing contents of MePWs, the PW leakage of the composites decreased, especially for PS MePW/PW/HTPB. Consequently, the MePW/PW/HTPB composites possess a potential application for PW-based polymer-bonded explosive system. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 46222.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
POLYMER
·
2022
·
IF 4.432
Bingxiu Xue,Fang He,Qi Lei,Xiaopeng Zhao,Jianbo Yin
Backbone, pendant and cross-linked poly(ionic liquid) (PIL) particles with the same ion pair and similar ion number density have been prepared and the electro-responsive electrorheological (ER) effect of their suspensions in insulating carrier liquid has been comparably studied by rheology and dielectric spectroscopy . It has found that the ER effect of cross-linked PILs is strongest and that of backbone PILs is weakest. Dielectric spectrum analysis has showed that the difference of ER effect is mainly due to the difference in the ion leakage and effective interfacial polarization of PIL particles under electric fields. Although the backbone PILs have the largest interfacial polarization strength , its ion leakage conductance is also largest and this results in its weakest ER effect. The cross-linked PILs well restrains the ion leakage conductance and the ER effect is largely enhanced. The difference in ion leakage conductance and ER effect is further related to the difference of PIL matrix structure according to X-ray diffraction characterization. This study clarified the influence of matrix structure on ER effect and dielectric polarization of PILs and provided a guidance for the design of PIL ER materials.
研究领域:
材料科学
·
物理学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）