PHARMAZIE
·
2021
·Vol. 76
·期 5
·195-201
·
IF 1.267
Dai Tianming,Wang Min,Wang Pengzhen,Dai Libing,Dai Renke,Meng Qingqi
The effects of eight oral anti-coronavirus drugs (lopinavir, ritonavir, chloroquine, darunavir, ribavirin, arbidol, favipiravir, oseltamivir) on the metabolism of four specific glycosides (polydatin, geniposide, quercitrin, glycyrrhizin) and on the activities of three major glycosidases
(β-glucosidase, α-rhamnosidase, β-glucuronidase) from gut microflora were explored in vitro and determined by LC-MS/MS. The metabolism of polydatin, geniposide, quercitrin and glycyrrhizin was significantly inhibited by one or several anti-coronavirus drugs of 100 μM
around 1 h and 4 h (P<0.05), among which darunavir could strongly reduce the production of genipin (70.6% reduction), quercitin (80.6% reduction) and glycyrrhetinic acid (37.9% reduction), which may cause a high risk of herb-drug interactions (HDI). Additionally, chloroquine reduced the
production of genipin and quercitin by more than 75% (P<0.05), whereas arbidol had no significant influence on the metabolism of polydatin, quercitrin and glycyrrhizin (P>0.05) so that its risk may be lower. The inhibition of darunavir on β-glucosidase was relatively strong (IC50
= 193±23 μM), and the inhibition became weaker on β-glucuronidase and α-rhamnosidase (IC50>500 μM). The consistency between gut microflora and glycosidase system indicated that the inhibition of darunavir on the activity of β-glucosidase and β-glucuronidase
may be the main reason for affecting the metabolism of geniposide, glycyrrhizin and polydatin in gut microflora. However, for the inhibition of darunavir and chloroquine on the metabolism of quercetrin, there was no correlation between gut microflora and α-rhamnosidase system. Assessing
the risk of HDI mediated by glycosidases in gut microflora may be conducive to the safety and efficacy of combining traditional herbal and Western medicine for the treatment of patients with Covid-19.
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PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Yifeng Xu,Yaxuan Liu,Chuanzhou Liang,Wenshan Guo,Huu Hao Ngo,Lai Peng
Information on biotransformation of antivirals in the side-stream partial nitritation (PN) process was limited. In this study, a side-stream PN sludge was adopted to investigate favipiravir biotransformation under controlled ammonium and pH levels. Results showed that free nitrous acid (FNA) was an important factor that inhibited ammonia oxidation and the cometabolic biodegradation of favipiravir induced by ammonia oxidizing bacteria (AOB). The removal efficiency of favipiravir reached 12.6% and 35.0% within 6 days at the average FNA concentrations of 0.07 and 0.02 mg-N L −1 , respectively. AOB-induced cometabolism was the sole contributing mechanism to favipiravir removal, excluding AOB-induced metabolism and heterotrophic bacteria-induced biodegradation. The growth of Escherichia coli was inhibited by favipiravir, while the AOB-induced cometabolism facilitated the alleviation of the antimicrobial activities with the formed transformation products. The biotransformation pathways were proposed based on the roughly identified structures of transformation products, which mainly involved hydroxylation, nitration, dehydrogenation and covalent bond breaking under enzymatic conditions. The findings would provide insights on enriching AOB abundance and enhancing AOB-induced cometabolism under FNA stress when targeting higher removal of antivirals during the side-stream wastewater treatment processes.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Shi-Ting Huang,Yong-Qian Lei,Peng-Ran Guo,Hong-Xin Chen,Shu-Chai Gan,Zeng-Hui Diao
In this study, a biomass-derived carbon block with heteroatoms (P and S) self-doped graphene structure was successfully synthesized by spent coffee grounds (SCG) directly sintered method. The spent coffee grounds biomass-derived carbon (SCC) block was used as electrocatalytic electrode to effectively degrade the antiviral drug of Favipiravir (FAV) in water, which represented excellent oxygen reduction reaction (ORR) activity with the H2O2 production. The doped heteroatoms in the graphene structure of SCC provided many active sites in the electrocatalytic electrode for ORR as well as more production of reactive OH･. Based on the structural characterization, radical species identification and intermediate products analysis, the reaction mechanism of FAV degradation by the SCC block electrode was proposed. This catalytic system also showed high ecological safety that the ecological toxicity for Vibrio fischeri was significantly reduced with the degradation process of FAV. The degradation of FAV in recycle test and in real sample of lake water, well water, tap water all demonstrated high stability of structure and catalytic activity of SCC. The electrocatalytic system based on SCC block electrode has realized the recycling and utilization of biomass resources and has promising application on the removal of environmental pollutants.
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高影响因子
TALANTA
·
2024
·
IF 5.6
Bing Zhao,Chan-Ru Xiong,Yao Liu,Qing-Cai Yu,Xing Chen
The outbreak of novel coronavirus pneumonia (COVID-19) in 2019 has garnered widespread attention. The virus exhibits high contagiousness, and in certain cases, it can lead to recurrent infections. Therefore, it is imperative to develop portable, sensitive, and accurate sensors to promptly detect infected individuals, control the virus's transmission, and determine suitable treatment strategies. In this study, we proposed a magnetically-assisted method employing CFO@CS-Au MNP as the substrate material, which was functionalized with human angiotensin-converting enzyme (ACE2) for efficient capture of SARS-CoV-2 spike protein in solution. Subsequently, the captured protein was sensitively detected through differential pulse voltammetry (DPV) electrical analysis. The linear detection range of the labeled GCE/MNP/GA/ACE2/BSA electrochemical sensor is from 1 pg/mL to 10 μg/mL, with a minimum detection limit of 0.15 pg/mL. Furthermore, the fabricated GCE/MNP/GA/ACE2/BSA sensor achieved satisfactory recoveries of SARS-CoV-2 spike protein in saliva and nasal swab samples within 10 min. These results indicate that this magnetically-assisted biosensor has established a solid foundation for the swift on-site detection of COVID-19.
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高影响因子
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