Nanomaterials
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2018
·Vol. 8
·期 8
·612
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IF 3.504
Dan Zhao,Wenting Ma,Xincai Xiao
The recognition of fingerprints has played an extremely important role in criminal investigations, due to its uniqueness. This paper reports on the recognition of sweat latent fingerprints using green-emitting, environment-friendly carbon dots prepared with DL-malic acid and ethylenediamine, and the exploration of impacting factors in the development process of fingerprints. The experiments showed that better fingerprint images could be obtained when the latent fingerprints are developed in green-emitting carbon dots with pH 9 for 30 min, at room temperature. The reported method was also effective for latent fingerprints on a variety of substrates, as well as for those water-immersed ones, where the developed fingerprint remained stable after long-term preservation. Furthermore, the fluorescent three-dimensional fingerprint image could provide direct and simple evidence on pressing habits. The objective of this paper was to present this method. The method may help to narrow the range of suspects during criminal investigations and in forensic science.Keywords:green;carbon dots;sweat latent fingerprints;forensic science
研究领域:
化学
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法医学
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DOI
PubMed
高影响因子
Journal of Forensic and Legal Medicine
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2024
·
IF 1.2
Zhonghao Yu,Yuhao Yuan,Qing Shi,Hang Gao,Erjuan Zhao,Jinghong Ma,Yiwu Zhou
Brodifacoum, a long-acting anticoagulant rodenticide, exerts inhibitory effects on blood coagulation factor synthesis, leading to abnormal clotting function and potential fatality. Poisoning of accidental exposure to brodifacoum is not rare, but death from brodifacoum poisoning can be largely avoided with timely and long-term effective treatments, consequently, forensic data on fatalities due to brodifacoum poisoning may be limited. This paper presents a case of brodifacoum-induced homicide, detailing the medial records and pathological changes observed in multiple organs. Furthermore, t liquid chromatography–tandem mass spectrometry (LC-MS/MS) was employed to measure the concentrations of brodifacoum in blood, hydropericardium, and urine 40 days post-poisoning (1 day post-mortem), yielding values of 0.097 μg/mL, 0.089 μg/mL, and 0.007 μg/mL respectively. The aim of this article is to contribute towards forensic identification of fatalities resulting from brodifacoum poisoning while also serving as a reference for clinical diagnosis in similar cases.
研究领域:
法医学
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毒理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
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2024
·Vol. 138
·期 4
·1323-1328
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IF 2.2
Yu Zhonghao,Zhao Erjuan,Shi Qing,Yuan Yuhao,Ma Jinghong,Zhou Luwang,Duan Yijie,Zhou Yiwu
Brodifacoum exerts its antagonistic effect against the metabolism of vitamin K, an essential component in the synthesis of blood coagulation factors. This effect ultimately hinders the blood’s capacity to clot effectively, rendering it a commonly employed rodenticide. Instances of lethal poisonings are exceedingly rare owing to expeditious medical intervention and treatment. Within this report, we present a case of brodifacoum-induced homicide, wherein the patient exhibited distinct clinical examinations and symptoms. Moreover, the patient’s blood sample exhibited a noteworthy brodifacoum concentration of 0.681 µg/mL even after a period of 43 days following the incident of poisoning. Although an autopsy was not conducted due to religious restrictions, we endeavor to reasonably deduce the cause of death and furnish corroborative evidence for clinical diagnosis, treatment, and forensic examination in instances involving brodifacoum poisoning.
研究领域:
医学
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毒理学
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法医学
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DOI
PubMed
高影响因子
Scientific Reports
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2024
·Vol. 14
·期 1
·1-9
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IF 3.8
Shi Qianwen,Wang Lele,Li Wenyue,Feng Yongming,Wei Zhiwen,Yun Keming
Nonoxidative ethanol metabolites have been reported as ethanol biomarkers in clinical and forensic cases. However, their forensic toxicokinetics are still unclear. The study aimed to simultaneously research the postmortem distribution and dynamic distribution of ethanol and its nonoxidative metabolites in 62 poisoned rabbits. Firstly, 32 rabbits were randomly divided into three groups and sacrificed after three doses ethanol, and their organs were collected to study the postmortem distribution of ethanol and its nonoxidative metabolites. The results showed that the distribution trends of ethanol and its nonoxidative metabolites were basically same between different groups, and ethanol could be better detected by collecting body fluids, while the nonoxidative metabolites could be better detected by collecting the heart, liver, kidney and testis. Secondly, 30 rabbits after 1/2LD 50 ethanol intragastric administration were sacrificed at 10 time points, and their organs were collected to study the dynamic distribution of ethanol and its nonoxidative metabolites. The results showed the concentrations of ethanol and its nonoxidative metabolites increased with time and then decreased. All analytes could be detected in all samples within 8 h, with the exception of ethyl oleate, which kept high level in muscle and vitreous humor, and could be detected within 4 h.
研究领域:
法医学
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毒理学
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DOI
PubMed
高影响因子
Forensic Science Medicine and Pathology
·
2015
·Vol. 11
·期 4
·570-576
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IF 1.983
Zhang Lin,Ma Jinghong,Li Shangxun,Xue Rui,Jin Ming,Zhou Yiwu
Diphenidol hydrochloride (DPN), a nonphenothiazinic antiemetic agent used primarily in patients with Meniere disease and labyrinthopathies to treat vomiting and vertigo, is considered to be a relatively safe drug. Since it was first approved in the United States in 1967, this drug has been widely used in Latin America and Asia and has contributed to sporadic suicidal and accidental poisonings in mainland China and Taiwan. However, its toxic or lethal concentration ranges have not yet been determined. We report a case of a 23-year-old female who suffered from DPN poisoning that resulted in death. At autopsy, there were no typical pathological findings, except for cerebral edema with high acetylcholinesterase expression. Postmortem analysis of DPN revealed 45 µg/ml in heart blood, 39 µg/ml in femoral vein blood, 141 µg/g in the liver, and 53 mg in the gastric contents. These concentrations indicated that the cause of death was DPN poisoning. The circumstances indicated that the manner of death was suicide. We also present a retrospective study, in which we review and summarize the literature from 1998 to 2014 and describe 16 cases of poisoning, including information from autopsy reports and postmortem drug concentrations. In forensic practice, drug residues at the scene, patients with convulsions and disturbance of consciousness, and rapidly occurring deaths, should draw attention to the possibility of this drug. Toxicological analysis and the exclusion of other diseases may ultimately be used to confirm DPN poisoning.
研究领域:
法医学
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毒理学
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临床医学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
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2021
·Vol. 135
·期 2
·431-440
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IF 2.686
Jiang Bowei,He Wei,Jin Chuan,Liu Ying,Wen Dan,Wang Chudong,ZEYE Moutanou Modeste Judes,Li Jienan,Zha Lagabaiyila
Genotyping of short tandem repeat (STR) markers is the basic method of forensic science. Enhanced technologies are needed to meet the requirements of databasing and casework samples. The STRscan-17LC kit is a 6 Dye STR kit which amplifies 16 STR loci: D3S1358, TPOX, D16S539, vWA, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, D18S51, TH01, D12S391, and D21S11 and the sex-determinant locus amelogenin. This kit is designed for better tolerance to PCR inhibitors and analysis of mildly degraded samples with all fragments smaller than 330 bases. In this study, the STRscan-17LC kit is validated according to the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines, including PCR-based studies, sensitivity, precision and accuracy, inhibitors, species specificity, DNA mixture studies, population, and concordance studies. The validation results suggest that the STRscan-17LC kit is a useful tool for forensic application.
研究领域:
法医学
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分子生物学
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遗传学
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DOI
PubMed
高影响因子
Forensic Science International-Genetics
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2020
·
IF 0
Jiashuo Zhang,Jingyi Zhang,Ruiyang Tao,Lei Jiang,Liqin Chen,Xuebo Li,Chengtao Li,Suhua Zhang
DNA profiling that relies on sets of highly polymorphic autosomal STR markers is widely used in the forensic field for human identification and paternity testing. However, the number of markers that are included in the STR kits that are currently available is insufficient to conclusively prove or disprove a relationship between individuals, especially when complex family scenarios are suspected or indirect analyses are required. In these cases, it becomes necessary to increase the number of loci under analysis to reach an adequate likelihood ratio (LR). In this study, we discovered 18 new autosomal non-CODIS STR loci (D1S1616, D1S1608, D2S437, D3S2457, D4S2406, D4S3249, D5S2843, D5S2501, D6S1010, D8S1039, D12S1301, D14S586, D15S815, SHGC-145653, CHLC.GATA14D12, D1S1603, HUMUT7148, and CHLC.GATA84D07) by web scanning and experimental screening. On the basis of this discovery, we developed a novel multiplex typing system named the “SiFaSTR 21plex_NCII Typing System” comprising 1) the 18 non-CODIS autosomal STRs mentioned above, 2) a CODIS locus of D2S1338, and 3) Amelogenin and DYS391. A forensic developmental validation, including sensitivity, species specificity, concordance, reproducibility, sample suitability, testing stability, and mixture testing, was performed following SWGDAM. The results of the validation studies indicated that this system is accurate, reliable and suitable for human DNA profiling. The sensitivity study of the system demonstrated that a full profile was obtainable with DNA as low as 125 pg. Species specificity was proven by the lack of cross-reactivity with a series of common animal species. The stability study demonstrated that 1 ng of control DNA could be fully genotyped with concentrations of haematin ≤ 150 μM, indigotin ≤ 5000 ng/μl, urea ≤ 16000 ng/μl, nigrosine ≤ 100 ng/μl and humic acid ≤ 20 ng/μl. In the mixture test, all of the minor alleles could be called at mixed ratios of 1:1, 1:3 and 3:1. We also investigated the allelic frequencies and forensic parameters of the included markers in 259 Chinese Han individuals. The forensic efficiency parameters, including the total power of discrimination (TDP) and the combined exclusion power in duos (CPE duos ) and in trios (CPE trios ) of the system were calculated to be greater than 0.9999999, 0.9997347 and 0.9999997, respectively. This result proved that the system is suitable for human identification and paternity testing. The 18 newly discovered non-CODIS STRs and the developed system will be a valuable supplementary tool for the forensic community and will help solve complex paternity cases, evolutionary studies and population investigations.
研究领域:
遗传学
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法医学
✓ 已验证
DOI
PubMed
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Jianhua Xiong,Shuquan Zhao,Xinwei Zhang,Yu Tian,Longda Ma,Yiwu Zhou,Chuixiu Huang
Liquid-phase microextraction (LPME) possesses a high potential to isolate organic substances from different sample matrices. In this work, LPME was applied for the first time to investigate the biodistribution of diphenidol in different biofluids, organs, and brain regions using a fatal poisoning case. Since the LPME of diphenidol hasn't been reported, the effect of supported liquid membrane (SLM), acceptor and donor phases, and extraction time on LPME performance was investigated first. The solvents of 2-nonanone and 2-nitrophenyl octyl ether (NPOE) were found to be stable and efficient SLMs for LPME of diphenidol from biofluids and tissue samples, respectively. At steady state, the LPME recoveries for different sample matrices were in the range of 87 %-91 %. Due to the clean-up capability of LPME and the relatively high concentration of diphenidol in the fatal poisoning case, the proposed LPME systems were validated with related sample matrices using HPLC-UV for the determination. The methods displayed good linearity (R² ≥ 0.9943), and the limits of detection were 0.30 mg L −1 , 0.28 mg L −1, and 2.7 μg g −1 for blood, urine, and liver samples, respectively. Meanwhile, the precision (≤13%), accuracy (90–110%), and matrices effect (±15%) were satisfactory at low, medium, and high concentrations. In addition, the stability, carryover, and dilution integrity met the requirements of ASB Standard 036. Finally, the proposed method was successfully applied to evaluate the biodistribution of diphenidol in five different biofluids, five organs, and six brain regions from a fatal poisoning case. Generally, the distribution of diphenidol in biofluids was lower than that in the organs and brain regions, and the highest concentration of diphenidol was observed in the liver, which is very important for the selection of inspection samples in forensic toxicological analysis. Therefore, LPME was proved to be a powerful tool for the investigation of biodistribution and postmortem redistribution in the fields of forensics.
研究领域:
分析化学
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法医学
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毒理学
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产品关联
DOI
PubMed
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
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2024
·
IF 3.4
Wu Shihao,Zhao Peng,Wang Aihua,Dong Linpei,Wu Xiaojun,Wang Jifen,Zhang Yunfeng
Monochromatic excitation X-ray fluorescence (ME-XRF) spectrometry emerges as a novel technique in trace element analysis, distinguished by its simplicity, rapidity, and efficiency. Its application in forensic toxicology has grown significantly for identifying toxic trace metals in biological samples. This study further expands its utility by developing a method for the rapid determination of arsenic (As), mercury (Hg), thallium (Tl), and lead (Pb) in hair samples. Calibration curves were established using analytical reference samples, and optimal detection conditions were determined and validated. The LODs for As, Hg, Tl, and Pb were 0.03, 0.03, 0.03, and 0.05 μg g −1 , respectively. Method precision was evaluated through seven parallel analyses of samples within the concentration range of 0.27 to 20.051 μg g⁻ 1 , with relative standard deviations (RSDs) consistently below 10%. The feasibility of the ME-XRF technique for forensic toxicology analysis was validated through the analysis of authentic poisoned hair samples collected from the animal experiment.
研究领域:
分析化学
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法医学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Environmental Sciences
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2025
·
IF 6.3
Wang Yu,Yuruo Wan,Wei Zhou,Jiayi An,Liting Tian,Jie Ma
Petroleum leakage is a major groundwater contamination source, with chemical composition of water soluble fractions (WSFs) from diverse oil sources significantly impacting groundwater quality and source identification. The aim of this study was to assess impact of 15 diverse oils on groundwater quality and environmental forensics based on oil-water equilibrium experiments. Our results indicate that contamination of groundwater by gasoline and naphtha is primarily attributed to volatile hydrocarbons, while pollution from diesel, kerosene, and crude oil is predominantly from non-hydrocarbons. Rapid determination of the extent of non-hydrocarbon pollution in WSFs was achieved through a new quantitative index. Gasoline and naphtha exhibited the highest groundwater contamination potential while kerosene and light crude oils were also likely to cause groundwater contamination. Although volatile hydrocarbons in the WSFs of diesel and jet fuel do not easily exceed current regulatory standards, unregulated non-hydrocarbons may pose a more severe contamination risk to groundwater. Notably, the presence of significant benzene and toluene, hydrogenation and alkylation products (e.g., C4-C5 alkylbenzenes, alkylindenes, alkyltetralins, and dihydro-indenes), cycloalkanes in WSFs can effectively be utilized for preliminary source identification of light distillates, middle distillates, and crude oils, respectively.
研究领域:
环境科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Nanomaterials
·
2021
·
IF 2.986
Yangxu Luo,Juan Du,Huadi Xiao,Ling Zheng,Xuncai Chen,Ande Ma,Qizhi Luo
Accurate identification and quantification of methamphetamine (MA) and its related substances are essential for the investigation and fair trial of drug offenses. In this study, a modified LC-ESI-MS/MS method for the simultaneous determination of MA and its isomer N-isopropylbenzylamine (N-IBA) in forensic samples was developed and validated. Optimum chromatographic separation of the target analytes was achieved on an Agilent Poroshell 120 SB-C18 column ( mm, 2.7 μm) at 40°C with isocratic elution at the flow rate of 0.40 mL/min. The mobile phase was acetonitrile and 20 mM ammonium acetate solution containing 0.1% formic acid (80 : 20, ). Positive ESI-MS/MS detection was performed in multiple reaction monitoring (MRM) mode to identify and quantify the target analytes. Method validation showed excellent linearity in the range of 0.51 ng/mL~51 ng/mL for MA and N-IBA. The low limit of detection (LLOD) and low limit of quantification (LLOQ) reached 0.1 ng/mL and 0.3 ng/mL for both analytes. The method showed a satisfactory accuracy with an inter- and intraday-relative error (RE) <20%, and a precision of inter- and intraday relative standard deviation (RSD) less than 15%. The validated method was successfully applied in real forensic samples and resulted in the detection of MA and N-IBA in 8 suspected samples in drug cases that only deemed MA positive using our previous routine screening procedure, which avoided the misidentification of N-IBA as MA.
研究领域:
分析化学
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法医学
✓ 已验证
产品关联
DOI
高影响因子
Molecular Genetics & Genomic Medicine
·
2020
·Vol. 8
·期 9
·e1419
·
IF 1.995
Shuangshuang Wang,Feng Song,Mingkun Xie,Ke Zhang,Bowen Xie,Zhanglong Huang,Haibo Luo
Background Short tandem repeat (STR) markers play a significant role in genetic applications and have proved to be effective for the personal identification in forensic medicine. In this study, a six-dye multiplex composed of 23 autosomal STR loci ( TH01 , D3S1358 , Penta D , D6S1043 , D21S11 , TPOX , D1S1656 , D12S391 , Penta E , D10S1248 , D22S1045 , D19S433 , D8S1179 , D2S1338 , D2S441 , D18S51 , vWA , FGA , D16S539 , CSF1PO , D13S317 , D5S818 , D7S820 ), one Y chromosome STR ( DYS391 ), two internal quality control markers (Quality Sensor QS1 and QS2), and Amelogenin was evaluated. Methods Evaluation studies, including PCR-based studies, sensitivity studies, species specificity studies, stability studies, DNA mixture studies, concordance studies, and precision evaluations were performed according to the guidelines of “Validation Guidelines for Forensic DNA Analysis Methods (2016)” by the Scientific Working Group on DNA Analysis Methods (SWGDAM). In addition, the forensic characteristics of 357 unrelated male samples from Han and Hui populations in China were investigated using 27 markers. Results Full STR profiles were obtained from different reaction volumes (5 ~ 25 μl), cycle numbers (28 ~ 34 cycles) and annealing temperatures (58 ~ 62°C). All STR profiles were obtained at humic acid concentration of up to 200 ng/μl and hematin concentration of up to 500 μM. No peaks were observed in most common animal samples except two innovative internal PCR controls (Quality Sensor QS1 and QS2). The six-dye multiplex showed a notably high value for the combined probability of exclusion (CPE), exhibiting values of with 0.99999999977688 in the Han population and 0.999999999583875 in the Hui population. The values of combined probability of discrimination (CPD) were 0.999999999999999999999999999997453 in the Han population and 0. 999999999999999999999999999994398 in the Hui population. In addition, concordance studies showed that there was no difference with the AGCU Express Marker 22 Kit. Conclusion The results indicated that the Investigator ® 26plex QS Kit is a robust, reliable, and suitable tool for forensic analysis and databasing.
研究领域:
法医学
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遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）