APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 102
·期 14
·6039-6046
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IF 3.34
Tigu Fitsum,Zhang Junli,Liu Guoxia,Cai Zhen,Li Yin
d -Pantothenic acid (vitamin B 5 ) has wide applications in the feed, food, chemical, and pharmaceutical industries. Its biological production routes which employ pantothenate synthetase (PS) as the key enzyme are attractive since they avoid the tedious and time-consuming optical resolution process. However, little data is available on the activity and kinetics of this enzyme, hampering the rational selection of an efficient enzyme for the biological production of d -pantothenic acid. In this study, six phylogenetically distant PS-encoding genes, from Escherichia coli , Corynebacterium glutamicum , Bacillus subtilis , Bacillus thuringiensis , Bacillus cereus , and Enterobacter cloacae , were expressed in E. coli . The PS from C. glutamicum exhibited a specific activity of 205.1 U/mg and a turnover number of 127.6 s -1 , which to our best knowledge are the highest values ever reported. The addition of substrates ( d -pantoic acid and β-alanine) to the E. coli strain harboring this enzyme during the early log phase of fermentation resulted in the production of 97.1 g/L of d -pantothenic acid within 32 h, corresponding to a conversion yield of 99.1% and a productivity of 3.0 g/L/h. To the best of our knowledge, this is the highest productivity reported to date.
研究领域:
生物化学
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微生物学
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工业生物技术
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2021
·
IF 4.24
Bo Zhang,Li Chen,Jie-Yi Jin,Na Zhong,Xue Cai,Shu-Ping Zou,Hai-Yan Zhou,Zhi-Qiang Liu,Yu-Guo Zheng
: As the key precursor of coenzyme A synthesis in vivo , D-pantothenic acid plays an important role in maintaining biological function, which is widely applied in the food , pharmaceutical, and cosmetic industries. At present, it was mainly produced by chemical methods, with serious environmental pollution, therefore, it is urgent to develop an economical, practical, and environmentally friendly biological fermentation method based on microbial chassis cells. Herein, based on our previously developed D-pantothenic acid producing strains, we obtained higher yielding D-pantothenic acid producing strains by systematic metabolic engineering . First, according to the results of CRISPRi experiments, lpd were overexpressed, disrupted the non-PTS (non-phosphoenolpyruvate-pyruvate transport system) as well as enhanced the PTS (phosphoenolpyruvate-pyruvate transport system) allowing precursor accumulation. Subsequently, based on the analysis of metabolic intermediates , the pyruvate bypass pathway was blocked as well as the (R)-pantothenic acid pathway was further enhanced, increasing the titer of D-pantothenic acid to 4.60 g/L. Finally, the organic acid and branched-chain amino acid synthesis pathways were blocked, and in order to accelerate the synthesis of acetolactate and the output of D-pantothenic acid, heterologous acetolactate synthase and pantothenate transfer protein were introduced, which significantly increased the yield of D-pantothenic acid, reach to 6.33 g/L. The final strain in a 5 L bioreactor produced 32.32 g/L D-pantothenic acid with a productivity of 2.33 g/L/h and the high-yielding strain provides an effective platform for biological fermentation to produce D-pantothenic acid.
研究领域:
生物技术
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代谢工程
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微生物学
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发酵技术
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2016
·
IF 2.196
Dandan Han,Yumin Liu,Xiaona Li,Teng Zhang,Zhenfang Li,Shichao Du,Mingxia Guo,Shijie Xu,Bo Yu,Baohong Hou,Junbo Gong
The solubility of calcium d -pantothenate in (water + methanol) mixtures and the effect of β -alanine on the solubility of calcium d -pantothenate in a constant mole ratio of methanol and water was measured from 263.15 K to 303.15 K by the HPLC analysis method ( P = 0.1 MPa). The solubility of calcium d -pantothenate increased with increasing temperature in all the mixtures at constant composition. Besides, it also increased with mole fraction of water in (water + methanol) mixtures at a constant temperature. Additionally, the solubility of calcium d -pantothenate was enhanced by β -alanine at a given temperature. The experimental solubility in (water + methanol) mixtures was correlated by the modified Apelblat equation, the ternary NRTL equation and Solubility-Polarity Model. The Apelblat equation, the quaternary NRTL equation and the Cohn equation were adopted to correlate the experimental solubility in the (water + methanol + β -alanine) mixtures. Worthwhile, the quaternary NRTL equation was first applied to correlating the quaternary solid-liquid equilibrium. It turned out that all the selected models give good performance on solubility prediction.
研究领域:
药物化学
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物理化学
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Yunhe Zhang,Yun Huang,Changjian Zhang,Yang Gao,Yanzhou Wang,Caixia Li,Yiheng Wang,Xing Li,Mingshan Wang,Yuanhua Lin
Aqueous zinc-ion batteries (AZIBs) have gained increasing attention for grid energy storage systems. However, ensuring the long-term reversible operation of the zinc anode remains a challenge due to dendrite growth and adverse side reactions during the charge and discharge cycles. This study investigates the use of d-pantothenic acid (D-PA) as an additive in 2 M ZnSO4 aqueous electrolyte to enhance the cycling stability of the zinc anode in AZIBs. Experimental results and theoretical calculations demonstrate that D-PA reshapes the solvation structure of Zn2+ by partially replacing coordinated water molecules, ensuring the stability of Zn2+ transport. Furthermore, D-PA adsorbs on active sites of the zinc anode, increasing the surface overpotential (|ηs|), reducing the nucleation energy barrier, and decreasing the critical nucleus size (rcrit), thus ensuring uniform zinc deposition. This dual role of modifying the Zn2+ solvation shell and regulating Zn2+ nucleation effectively mitigates dendrite growth and suppresses side reactions, resulting in excellent stability of the zinc anode. Consequently, Zn||Zn symmetrical cells with the D-PA additive maintain stable operation for over 2000 h at 1.0 mA cm–2 and 1.0 mA h cm–2, and nearly 4000 h at 4.0 mA cm–2 and 4.0 mA h cm–2. Additionally, Zn||Cu asymmetric cells exhibit cycling stability over 300 cycles at 0.5 mA cm–2 and 0.5 mA h cm–2, with an average Coulombic efficiency of 99.29%. Moreover, Zn||V2O5 full cells containing the D-PA additive exhibit stable cycling performance over 1000 cycles at a current density of 1 A g–1, maintaining a high capacity retention. Specifically, the initial capacity of the full cell is around 161.17 mA h g–1, with approximately 62.7% capacity retention after 1000 cycles.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
LIFE SCIENCES
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2020
·
IF 3.647
Zhuo Wang,Weixiao Nan,Huazhe Si,Shiyong Wang,Haihua Zhang,Guangyu Li
Aims Pantothenic acid (PA) has been applied to treat alopecia, but the underlying mechanism is still unclear. Our study aims to explore the underlying mechanism of PA in regulating hair follicle (HF) growth. Main methods Mink HFs and dermal papilla (DP) cells were isolated and cultured in vitro . HFs and DP cells were treated with 0, 10, 20, 40 μg/ml PA. The effect of PA on HF growth, DP cell proliferation, cell cycle distribution, cell migration, and insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) expressions in DP cells was measured. Moreover, the effect of PA on inhibitor of DNA binding 3 (ID3)/Notch signaling pathway was analyzed. Subsequently, ID3 was silenced to validate whether ID3/Notch signaling pathway was involved in regulating DP cell proliferation by PA. Key findings Both 20 μg/ml and 40 μg/ml PA promoted HF growth, G1/S transition of DP cells and IGF-1 and VEGF expressions in DP cells, while only 20 μg/ml PA promoted cell viability and the migration of DP cells. Thus 20 μg/ml PA was chosen for the following experiments. PA treatment was found to up-regulate ID3 expression but down-regulate Notch receptor 1 (Notch1) and Notch signaling targets expressions. Furthermore, ID3 knockdown reversed PA-induced cell proliferation and inhibition of Notch1 and Notch signaling targets expressions, indicating that PA-induced DP cell proliferation and inhibition of Notch signaling were mediated via up-regulation of ID3. Significance This study provides an underlying mechanism related to the effect of PA on stimulating DP cell proliferation.
研究领域:
皮肤科
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Jiaxuan Guo,Xixi Sun,Yujie Yuan,Qitong Chen,Zutian Ou,Zixin Deng,Tian Ma,Tiangang Liu
Vitamin B5, also called d-pantothenic acid, is an essential vitamin in the human body and is widely used in pharmaceuticals, nutritional supplements, food, and cosmetics. However, few studies have investigated the microbial production of d-pantothenic acid, especially in Saccharomyces cerevisiae. By employing a systematic optimization strategy, we screened seven key genes in d-pantothenic acid biosynthesis from diverse species, including bacteria, yeast, fungi, algae, plants, animals, etc., and constructed an efficient heterologous d-pantothenic acid pathway in S. cerevisiae. By adjusting the copy number of the pathway modules, knocking out the endogenous bypass gene, balancing NADPH utilization, and regulating the GAL inducible system, a high-yield d-pantothenic acid-producing strain, DPA171, which can regulate gene expression using glucose, was constructed. By optimizing fed-batch fermentation, DPA171 produced 4.1 g/L d-pantothenic acid, which is the highest titer in S. cerevisiae to date. This study provides guidance for the development of vitamin B5 microbial cell factories.
研究领域:
生物技术
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代谢工程
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微生物生物技术
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2023
·Vol. 11
·期 26
·14311-14319
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IF 11.9
Lantao Liu,Ziyu Sun,Yaping Lu,Jiapeng Zhang,Yiming Li,Gaixia Zhang,Xiaohong Chen,Sasha Omanovic,Shuhui Sun,Huaihe Song
Due to the environmental friendliness and high safety of aqueous Zn-ion hybrid capacitors (ZIHCs), the exploration of high-performance porous carbon electrodes for ZIHCs has recently attracted significant attention. However, most of the reports focus on the preparation method and modification of porous carbon, and rarely explore the mechanisms of material synthesis/fabrication in the carbonization process. In this study, we developed a simple self-template method for preparing N-doped porous carbon from D-calcium pantothenate (DCP). More importantly, the morphology evolution, heteroatom doping, and defect generation of the DCP during carbonization are clarified. Based on a clear understanding of the carbonization mechanism, assisted by DFT theoretical simulations, we find the optimal carbonization temperature of the DCP-derived porous carbon for ZIHCs. The assembled ZIHCs exhibit excellent rate performance (140 mA h g−1 at 0.2 A g−1 and 86.2 mA h g−1 at 6.4 A g−1), and high energy density and power density (111.1 W h kg−1 and 4835.2 W kg−1, respectively). This work provides a constructive avenue for designing high-performance ZIHC electrode materials.
研究领域:
材料科学
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电化学
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能源存储
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纳米材料
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碳材料
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电化学储能
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2023
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IF 3.9
Xiao-Jian Zhang,Min Cao,Yi-Fei Luo,Meng-Yu Huang,Fang-Ying Zhu,Zhi-Qiang Liu,Yu-Guo Zheng
D-pantolactone (D-PL) is the crucial chiral intermediate for the synthesis of D-pantothenic acid. The asymmetric synthesis of D-PL from ketopantothenic acid lactone (KPL) using conjugated polyketide reductases (CPRs) exhibited high catalytic efficiency and high enantioselectivity. A novel conjugated polyketide reductase KbCPR was isolated from Kazachstania barnettii and the mutant KbCPR Y29K/R65N/K215R was developed with the improved activity by 9.0 folds and the enhanced k cat / K m value by 2.6 folds than the wild-type based on integrated substrate pocket alteration strategy, to alter the substrate channel, to regulate the pocket size, and to facilitate proton transfer. An organic-aqueous biphase biosystem containing 40% dibutyl phthalate (v/v) was constructed to inhibit the hydrolysis of KPL and improve the catalytic efficiency. The time-space yield of 892.9 g L -1 d -1 was achieved, which was higher than ever reported. Furthermore, the organic-aqueous biphase system was employed for the first time in the multi-enzymatic system for D-PL preparation from L-pantolactone (L-PL), without any accumulation of KPL intermediate, high concentration of 1 M L-PL was 95.5% converted to D-PL, with the final production e.e. of >99.9%.
研究领域:
生物催化
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酶工程
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有机化学
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生物技术
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产品关联
DOI
高影响因子
Cell Death Discovery
·
2025
·Vol. 11
·期 1
·1-15
·
IF 7
Cheng Mengting,Wu Wentong,Li Qing,Tao Xinyu,Jiang Feng,Li Jinjin,Shen Nonger,Wang Fei,Luo Peihua,He Qiaojun,huang Ping,Xu Zhifei,Zhang Yiwen
Sotorasib, the inaugural targeted inhibitor sanctioned for the management of patients afflicted with locally advanced or metastatic non-small cell lung cancer presenting the KRAS G12C mutation, has encountered clinical application constraints due to its potential for cardiac injury as evidenced by safety trials. This investigation has elucidated that the heightened expression of neuraminidase-1 (NEU1) constitutes the principal etiology of cardiac damage induced by sotorasib. Mechanistically, sotorasib treatment inhibited the ubiquitinated degradation of NEU1, leading to its elevated expression, which induced downstream AKT signaling pathway inhibition and mitochondrial dysfunction leading to cardiomyocyte apoptosis. Meanwhile, in vivo and in vitro studies showed that D-pantothenic acid (D-PAC) alleviated sotorasib-induced cardiac damage by promoting NEU1 degradation. In conclusion, this study revealed that NEU1 is a key protein in sotorasib cardiotoxicity and that reducing the level of this protein is a critical strategy for the clinical treatment of sotorasib-induced cardiac injury. Schematic representation of a mechanism.
研究领域:
肿瘤学
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心脏病学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Qiyan Lyu,Lei Chen,Shiye Lin,Hui Cao,Hui Teng
The present study aims to design a self-microemulsion delivery system (d-α-tocopheryl polyethylene glycol 1000 succinate - quillaja saponin) to enhance the absorptivity of dihydromyricetin (DMY-S), and to investigate its dietary intervention effect on high-fat-diet (HFD) fed mice. We find DMY-S can inhibit the increase of body weight and fat mass, preventing non-alcoholic fatty liver disease. Compared to the model group, the abundance of mice intestinal flora is mainly changed in certain bacterial genera of Firmicutes and Bacteroides, including norank_f_Muribaculaceae and Blautia. The result of metabolism analysis indicated that the expression levels of cincassiol B, creatine, pantothenic acid and aminobutyric acid in the liver tissues of mice treated with DMY-S showed a down-regulation. The DMY-S prevented hyperlipidemia in HFD mice mainly by affecting different pathways including glycerophospholipid metabolism, sphingolipid metabolism and pantothenate and CoA biosynthesis.
研究领域:
药剂学
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营养学
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代谢组学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2023
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IF 5.6
Jirui Lang,Li Li,Yunyun Quan,Ruirong Tan,Jinbiao Zhao,Min Li,Jin Zeng,Shilong Chen,Ting Wang,Yong Li,Junning Zhao,Zhujun Yin
Wuwei Shexiang Pill (WSP) is a Tibetan traditional medicine, which has been demonstrated to exhibit potent anti-inflammatory and anti-gout effects. However, the specific pharmacological mechanism is not elucidated clearly. In the present study, liquid chromatography-mass spectrometry (LC-MS)-based metabolomics was applied to investigate the alteration of serum metabolites induced by WSP treatment in MSU-induced gouty rats. Subsequently, bioinformatics was utilized to analyze the potential metabolic pathway of the anti-gout effect of WSP. The pharmacodynamic data discovered that WSP could ameliorate ankle swelling and inflammatory cell infiltration, as well as downregulate the protein expression of IL-1β, p-NF-κB p65, and NLRP3 in the synovial membrane and surrounding tissues of gouty ankles. LC-MS-based metabolomics revealed that there were 30 differential metabolites in the serum between sham-operated rats and gouty ones, which were mainly involved in the metabolism of fructose and mannose, primary bile acid biosynthesis, and cholesterol metabolism. However, compared to the model group, WSP treatment upregulated 11 metabolic biomarkers and downregulated 31 biomarkers in the serum. KEGG enrichment analysis found that 27 metabolic pathways contributed to the therapeutic action of WSP, including linoleic acid metabolism, phenylalanine metabolism, and pantothenate and CoA biosynthesis. The comprehensive analysis-combined network pharmacology and metabolomics further revealed that the regulatory network of WSP against gout might be attributed to 11 metabolites, 7 metabolic pathways, 39 targets, and 49 active ingredients of WSP. In conclusion, WSP could ameliorate the inflammation of the ankle in MSU-induced gouty rats, and its anti-gout mechanism might be relevant to the modulation of multiple metabolic pathways, such as linoleic acid metabolism, phenylalanine metabolism, and pantothenate and CoA biosynthesis. This study provided data support for the secondary development of Chinese traditional patent medicine.
研究领域:
药理学
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代谢组学
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传统中药
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炎症生物学
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生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Cheng Zhao,Peng Chu,Xiaodong Tang,Jie Yan,Xiaomen Han,Jie Ji,Xianhui Ning,Kai Zhang,Shaowu Yin,Tao Wang
This study evaluated the effects of chronic exposure to copper nanoparticles (Cu-NPs) and waterborne copper (CuSO 4 ) on the reproductive system of yellow catfish ( Pelteobagrus fulvidraco ). Juvenile yellow catfish were exposed to 100 and 200 μg Cu/L Cu-NPs and 100 μg Cu/L CuSO 4 for 42 days. The results showed clear reproductive defects in both female and male yellow catfish in the 200 μg Cu/L Cu-NPs and 100 μg Cu/L CuSO 4 groups. Exposure to Cu-NPs or CuSO 4 inhibited folliculogenesis and vitellogenesis in the ovaries, and spermatogenesis in the testes , accompanied by elevation of the apoptotic signal. Ultrastructural observations also revealed damaged organelles of gonadal cells in both testes and ovaries. Most of the hypothalamic-pituitary-gonadal (HPG) axis genes examined and serum sex steroid hormones tended to be downregulated after Cu exposure. Metabolomic analysis suggested that gonadal estradiol level is sensitive to Cu-NPs or CuSO 4 . The heat map of gonadal metabolomics suggested a similar effect of 200 μg Cu/L Cu-NPs and 100 μg Cu/L CuSO4 in both the ovaries and testes. Additionally, metabolomics data showed that the reproductive toxicity due to Cu-NPs and CuSO 4 may occur via different metabolic pathways. Cu-NPs tend to dysregulate the metabolic pathways of sphingolipid and linoleic acid metabolism in the ovary and the biosynthesis of amino acids and pantothenate and CoA in the testis. Overall, these findings revealed the toxicological effects of Cu-NPs and CuSO 4 on the HPG axis and gonadal metabolism in yellow catfish.
研究领域:
环境毒理学
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水生生物学
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生殖生物学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）