JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Dongping Zeng,Fada Zhou,Wei Wang,Zheng Tang,Linxing Luo,Jiamin Li
Needle-free injection technology possess several advantages over traditional needle injections. This type of device is designed to achieve drug delivery by interacting with the skin through a high-velocity microjet stream, rather than delivering the liquid drug through a hollow needle. This work focuses on the influence of the jet velocity and interlayer combinations of the penetrated medium on the injection process of the pneumatic needle-free injection. Skin tissues with different Young's modulus and tissue layer thicknesses were modelled by varying the combination of gels with different mass fractions (from 0.5 % to 6.0 %) or tissue layer thicknesses (from 5 mm to 20 mm) in single-layered, two-layered and multi-layered gels, respectively. The high-speed imaging data showed that the injection process of jets generated by pneumatic needle-free injection into gels can be divided into three phases: erosion, stagnation and diffusion. Meanwhile, the thickness of the subcutaneous layer of the multi-layered gel was 5 mm–10 mm corresponding to intramuscular injection and 20 mm or more corresponding to subcutaneous injection at a jet velocity of 127.44 m/s. It was also found that the jet velocity can be adjusted according to the mechanical properties of the skin tissue, allowing subcutaneous or intramuscular injection. Our findings contribute to a better understanding of the injection mechanism of the pneumatic needle-free injection in skin with different mechanical properties.
研究领域:
生物力学
·
医学工程
·
药剂学
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产品关联
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Tian Ou,Chao Fang,Shunke Ding,Tiantao Zhao,Shaogang Liu,Zhenxun Yu,Wenhai Chu
Syringe filters are widely used for sample pretreatments in laboratories. This study found that, surprisingly, these filters can leak dissolved organic carbon (DOC) that can potentially serve as precursors of disinfection by-products (DBPs). Nine common types of syringe filters were assessed. The results showed that the DOC of ultrapure water increased after syringe filtration. The DOC shed from filter membranes was characterized, whose spectra showed that the main compounds exhibited a low apparent molecular weight . Five classes of DBPs were investigated including trihalomethanes, haloacetaldehydes, haloacetonitriles, haloacetamides and halonitromethanes, among which trichloromethane (TCM), dichloroacetaldehyde (DCAL), trichloroacetaldehyde (TCAL), dichloroacetonitrile (DCAN), and trichloronitromethane (TCNM) were principally detected. The DBP formation was affected by chlorination time and membrane types. In general, the use of the poly vinylidene fluoride membrane resulted in the highest formation of TCM and TCAL, whereas nylon and mixed cellulose esters membranes contributed significantly to the formation of DCAN and TCNM, respectively. The shedding DOC and the formation of TCM, DCAL and TCAL from filter membranes were mitigated effectively by pre-washing; however, the contribution of membrane leakage to DCAN and TCNM formation was still notable, even with a pre-wash volume of 50 mL. When unwashed syringe filters were used for a real water sample, the DBP formation increased by up to 73.2% compared to the pre-washed ones; particularly for TCNM it was always over 15%. Therefore, for better quality control in laboratories, more attention should be paid to the syringe filters during sample pre-treatments, particularly when DBP formation is being investigated.
研究领域:
环境科学
·
公共卫生
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2018
·Vol. 41
·期 8
·1856-1863
·
IF 2.415
Xiaoqiong Zhang,Peiyi Wang,Qiang Han,Hengzhen Li,Tong Wang,Mingyu Ding
In-syringe solid-phase extraction is a promising sample pretreatment method for the on-site sampling of water samples because of its outstanding advantages of portability, simple operation, short extraction time, and low cost. In this work, a novel in-syringe solid-phase extraction device using metal–organic frameworks as the adsorbent was fabricated for the on-site sampling of polycyclic aromatic hydrocarbons from environmental waters. Trace polycyclic aromatic hydrocarbons were effectively extracted through the self-made device followed by gas chromatography with mass spectrometry analysis. Owing to the excellent adsorption performance of metal–organic frameworks, the analytes could be completely adsorbed during one adsorption cycle, thus effectively shortening the extraction time. Moreover, the adsorbed analytes could remain stable on the device for at least 7 days, revealing the potential of the self-made device for on-site sampling of degradable compounds in remote regions. The limit of detection ranged from 0.20 to 1.9 ng/L under the optimum conditions. Satisfactory recoveries varying from 84.4 to 104.5% and relative standard deviations below 9.7% were obtained in real samples analysis. The results of this study promote the application of metal–organic frameworks in sample preparation and demonstrate the great potential of in-syringe solid-phase extraction for the on-site sampling of trace contaminants in environmental waters.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Cuili Li,Maojie Xiang,Jie Liu,Guanglei Fu
Quantitative testing of cancer biomarkers is of significance in early diagnostics of the disease. Herein, we developed an all-in-a-syringe immunoassay using autoinjected visual distance readout for simple quantitative testing of prostate specific antigen (PSA) as a model target. The whole immunoassay protocol was conducted in a single disposable syringe, while employing it as the host of the immuno-recognition system, the tool for reagent handling and autoinjected distance readout of the assay signals. The sandwich-type immuno-recognition system was constructed on the inner polypropylene wall of the syringe cylinder. In the presence of H 2 O 2 , the peroxidase-mimic activity of immuno-captured platinum nanoparticles (Pt NPs) in the cylinder drove the automatic injection of liquids into the catheter of the syringe, quantitatively displaying the assay signals as autoinjected visual distance parameters. The assay was integrated into a three-dimensional (3D)-printed device for easier manipulation. Without the aid of any laboratory pipettes, specialized detectors and complex sealing treatments of the device, PSA was detected at a limit of detection (LOD) of 0.43 ng/mL with acceptable accuracy in testing clinical serum samples. This is the first exploration of all-in-a-syringe for quantitative immunoassay, which possesses great potential for simple testing of cancer biomarkers, given the versatility of syringes.
研究领域:
医学诊断
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Mengchan Xu,Wei Mao,Tao Hu,Mengyuan Xu,Xingwei Cai,Wei Shen,Sheng Tang,Haiwei Shi,Li Tan,Chang Liu
In this work, a syringe needle-based integrated method was designed for the detection of biogenic amines (BAs) in raw meat samples. Based on a sequential process, the needle-based sampling, micro liquid-phase extraction and peroxidase-like catalysis were adopted for the sample collection, target analytes extraction and colorimetric analysis, respectively. The proposed method exhibited high selectivity towards BAs (the total amount of histamine, putrescine and cadaverine was utilized to present the level of BAs), where the linear range is 5–50 μM and 50–1000 μM, and the limit of detection is 1.52 μM. Specifically, the whole process could be completed in a single syringe needle. In addition, due to the minimized sampling, the change of BAs levels with time in different area of real samples (fish) can be conveniently investigated. This method has the advantages of simplicity, low cost, high sensitivity and selectivity, endowing it a promising candidate for food analysis.
研究领域:
食品科学
·
分析化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2018
·
IF 0.827
Jianlin Zhuang,Jinmao You,Shijuan Zhang,Zhiwei Sun,Zhongyin Ji,Jiamin Liu,Yanxin Yu
A new fluorescence labeling reagent 1-(4-(1 H-phenanthro[9,10-d]imidazol-2-yl)phenyl)-1 H-pyrrole-5-dione (PIPD) used for the sensitive determination of thiols in food samples has been successfully prepared. Thiols were extracted by a gas purge microsyringe extraction (GP-MSE) device. The derivatization of PIPD with thiols could be finished within 10 minutes at 35 °C in weak alkaline condition, and the corresponding derivatives showed excellent fluorescence properties with excitation and emission wavelengths of 264 nm and 396 nm, respectively. The eight thiol derivatives could be easily separated on normal reversed phase column because of the introduction of highly hydrophobic PIPD unit into the target analytes. It is the first time that GP-MSE has been applied to the extraction of a wide range of thiols in food samples with only a small amount of methanol (0.5 mL) needed. The limits of detection (LODs) were in the range of 0.19–0.98 µg/kg, while the recoveries were in the range of 85.6%–97.1% with relative standard deviations (RSDs) < 4.98%. Good linearities were observed for all analytes with correlation coefficients of higher than >0.9995.GRAPHICAL
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2020
·
IF 10.317
Jingli Ren,Xingjie Yin,Yu Chen,Yu Chen,Hua Su,Ke Wang,Lianbin Zhang,Jintao Zhu,Chun Zhang
Tissue puncture is an effective means in the diagnosis and treatment of diseases, providing important information for clinical doctors. Yet, haemorrhage is still a main and unavoidable complication, which can cause serious outcomes, especially in patients with abnormal coagulation function. Herein, we present a facile yet effective strategy to prepare haemostatic needles by coating the syringe needles surface with alginate-CaCl 2 -based hydrogel with carefully optimized mechanical and chemical properties, which can effectively prevent bleeding following tissue puncture. The needle with haemostatic coating exhibits complete prevention of blood loss following vessel and viscera puncture in the normal animal as well as in animal models with hemorrhagic diathesis. The syringe needle with haemostatic coating in this work holds enormous potentials for clinical applications, including sampling and injection.
研究领域:
生物医学工程
·
血液学
·
外科技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Li Cuili,Xiang Maojie,Liu Jie,Zhang Shuting,Li Haojie,Fu Guanglei
Pressure-based detection has drawn increasing interest for developing immunoassays but is still challenged in point-of-care testing (POCT). In this work, a pressure-based immunoassay “lollipop” was developed using syringe-autoinjected visual distance readout. The “lollipop” consisted of a disposable syringe “stick” as the immuno-recognition host and a chip “head” as the signal readout element. Using urinary microalbuminuria (mALB) as a model target, we constructed the immunoassay system on the interior cylinder surface of the syringe. In the presence of H 2 O 2 , the peroxidase-mimicking activity of platinum nanoparticles (Pt NPs) immuno-captured in the cylinder drove the automatic injection of dye liquids from the syringe into a spiral capillary catheter in the chip. The concentration of mALB in the “lollipop stick” was thereby visually displayed as the syringe-autoinjected distance signals in the “lollipop head”. The assay exhibited a limit of detection (LOD) of 5 ng/mL for mALB quantification and linear response in a range from 0.006 μg/mL to 6 μg/mL with reliable accuracy in clinical urine sample analysis. Taking advantage of the versatility of syringes, the immunoassay “lollipop” works without the aid of any laboratory liquid handlers (e.g., pipettes), specific detectors (e.g., manometers) and complex chip operations (e.g., reagent loading), showing great promise in POCT.
研究领域:
生物分析化学
·
即时检测
·
免疫诊断
·
纳米技术
·
微流控技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 28
·8071-8079
·
IF 4.478
Wei Wei,Lu Zenghui,Wu Ting,Wang Haibo,Han Qiang,Liang Qionglin
In this study, a covalent organic framework (COF)-TpBD-supported melamine sponge (MS) was fabricated through a one-step hydrothermal method. The obtained monolithic column was then applied in in-syringe solid-phase extraction (IS-SPE) for the separation of three volatile ingredients from serum samples. Given credit for the superior adsorption capacity of the COF and the homogeneous microporous property of MS, the developed column exhibited satisfactory separation of the targets. And the dominating adsorption mechanism was the hydrophobic interaction forces between TpBD and targets and the high mass transfer efficiency provided by the large pore structure of MS. The results of dynamic adsorption showed that the MS@TpBD column displayed much better adsorption performance than blank MS and TpBD. And it has featured great reusability up to 5 cycles and obtained satisfied recovery values (87.9 ~ 110.3%) in serum samples. As a result of sample clean-up, this column offers low limit of detections (LODs) down to 0.014, 0.010, and 0.020 μg/mL, respectively. In summary, we believe that this convenient separation column has prominent application promise in the fields of separating activity ingredients in biological samples.
研究领域:
分析化学
·
药学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
LAB ON A CHIP
·
2021
·Vol. 21
·期 18
·3429-3437
·
IF 6.799
Tengbao Xie,Ping Wang,Lei Wu,Bangyong Sun,Qiang Zhao,Gang Li
In this work, we present a simple and equipment-free system for discretizing samples into tens of thousands of discrete volumes in tens of seconds. Unlike conventional sample discretization systems that require bulky syringe pumps, pressure controllers, or vacuum equipment, our system requires only a sheet of water-soluble film, a hand-operated syringe, and a microfluidic device containing a high-density microchamber array. In this system, the water-soluble film seals the device inlet to form a closed channel–chamber system, while the syringe is used to create a vacuum in the closed system. Benefitting from the high negative pressure created by syringe-vacuum and the dissolution-triggered gating mechanism of the sealing water-soluble film, the aqueous sample loaded into the device inlet can be rapidly partitioned into tens of thousands of isolated chambers without the need for any expensive pumping systems. We demonstrated the utility of this system by exploiting it for digital PCR. We believe that this simple discretization system will find broad applications, such as in digital bioassays, single-cell analysis, and point-of-care diagnostics.
研究领域:
微流控技术
·
生物技术
·
即时诊断
·
数字生物分析
·
单细胞分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-9
·
IF 5.3
Liu Shendong,Chen Xinlian,Zhao Hong,Lin Tianran,Hou Li,Zhao Shulin
An ultrasensitive photothermal assay was designed for point-of-care testing (POCT) of tumor markers based on a filter membrane. Firstly, Cu 2-x Se was successfully encapsulated in liposome spheres with biotin on the surface and connected to carcinoembryonic antigen (CEA) aptamer with 3'end modified biotin by streptavidin. Secondly, the CEA antibody was successfully modified on the surface of the nitrocellulose membrane through simple incubation. Finally, the assay process was completed using a disposable syringe, and the temperature was recorded using a handheld infrared temperature detector. In the range 0–50 ng mL −1 , the temperature change of the nitrocellulose membrane has a strong linear relationship with CEA concentration, and the detection limit is 0.097 ng mL −1 . It is worth noting that the entire testing process can be easily performed in 10 min, much shorter than traditional clinical methods. In addition, this method was successfully applied to the quantitative determination of CEA levels in human serum samples with a recovery of 96.2–103.3%. This rapid assay can be performed by “one suction and one push” through a disposable syringe, which is simple to operate, and the excellent sensitivity reveals the great potential of the proposed strategy in the POCT of tumor biomarkers. Graphical Abstract
研究领域:
生物医学工程
·
分析化学
·
即时诊断
·
癌症生物标志物
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Xiao-Mei He,Xi Chen,Gang-Tian Zhu,Qian Wang,Bi-Feng Yuan,Yu-Qi Feng
Sample preparation methods with high selectivity, efficiency, and matrix resistance are essential for phosphoproteomic analysis. In this study, carboxyl cotton chelator-titanium(IV) (CCC-Ti4+) fibers, a novel CCC-based fibrous sorbent with excellent biocompatibility, were successfully synthesized on the basis of the coordination effect between double carboxyl groups on CCC and Ti4+. The synthesis of CCC-Ti4+ fibers was easy, and the incorporated titanium content was high. On the basis of immobilized metal ion affinity chromatography (IMAC), CCC-Ti4+ fibers were used for specific capture of phosphopeptides using a lab-in-syringe solid-phase extraction (SPE) from multiple biological samples, including standard protein digests, nonfat milk digests, human serum, and animal tissue. The proposed sorbent exhibited high selectivity (β-casein/BSA = 1:1000) and good sensitivity (10 fmol) in phosphopeptides analysis. Meanwhile, the lab-in-syringe SPE greatly simplified the entire process of enrichment. Thanks to the good biocompatibility of CCC-based material, CCC-Ti4+ fibers showed excellent performance in phosphopeptide enrichment from protein-rich human serum. Finally, CCC-Ti4+ fibers were applied for selective capture of phosphopeptides from tryptic digests of rat brain lysate followed by LC-MS/MS analysis. Using the proposed method, we identified 3950 unique phosphosites from 1 mg of rat brain in a single experiment, which is much better than previously reported IMAC-based strategies. Taken together, this efficient method will find broad application in large-scale phosphoproteomics analysis because of the rapid (3 min) convenient procedure and excellent performance.
研究领域:
分析化学
·
蛋白质组学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）