npj Flexible Electronics
·
2022
·Vol. 6
·期 1
·1-8
·
IF 12.019
Lin Baojun,Wang Meng,Zhao Chao,Wang Shijie,Chen Kai,Li Xiao,Long Zaishang,Zhao Chenxu,Song Xinyue,Yan Sen,Wang Laili,Ma Wei
Smart contact lens has drawn extensive research interests due to the noninvasive real-time detection of the human body to provide biomedical information for health management. However, it has been difficult to accurately measure the physiological signals in tears, and the use of external power source has also hindered the future applications. Here, we demonstrated an organic electrochemical transistor based multiplexed sensors self-powered by the organic solar cells (OSCs). The integrated device was fabricated via simple process including solution blade-coating and thermal evaporation. OSCs were optimized to provide optimal operation voltage for the sensors that exhibit semilog-linear response to the glucose and calcium ions in tear fluids without any peripheral circuits. The sensing signals can be transmitted to the laptop wirelessly through a near filed communication unit. This integrated self-powered multiplexed sensing device will provide real-time monitoring of the biomarkers in tears, prospected to be installed on the smart contact lens for the early detection and diagnosis of diabetes.
研究领域:
生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
ACS Omega
·
2018
·
IF 0
Cheng Chen,Zhi-Qiang Dong,Jian-Hua Shen,Hao-Wen Chen,Yi-Hua Zhu,Zhi-Gang Zhu
Photonic crystal (PC) materials have huge potentials as sensors for noninvasive and real-time monitoring glucose in tears. We developed a glucose-sensitive PC material based on monolayered colloidal crystals (MCCs). Polystyrene nanoparticles were first self-assembled into a highly ordered MCC, and this two-dimensional (2D) template was then coated by a 4-boronobenzaldehyde-functionalized poly(vinyl alcohol) hydrogel. Such a sensor efficiently diffracts visible light, whose structural color could change from red through yellow to green, as the glucose concentration altered from 0 to 20 mM, covering both tears’ and bloods’ physiological ranges. The sensor also represents a rapid response within 180 s at each titration of glucose, combining the characteristics of high accuracy and sensitivity in detecting the glucose concentration in tears, and this intelligent sensing material presents certain possibility for the frontier point-of-care glucose monitoring.
研究领域:
生物医学工程
·
材料科学
·
传感技术
✓ 已验证
产品关联
DOI
PubMed
Separations
·
2022
·Vol. 9
·期 10
·284
·
IF 3.344
Jiaxin Huang,Chunxian Tao,Zhenqing Li,Erika Miyake,Qingxiang You,Wen Xiao,Dawei Zhang,Yoshinori Yamaguchi
Capillary electrophoresis (CE) is a versatile analytical separation method in the field of biochemistry. Although it has been proved that the relative molecular mass (Mr) of the polymers determines the threshold concentration of the entangled polymer solution, which will affect the separation performance of DNA molecules, there is still no report on the effect of Mron the separation performance of proteins. Herein, we have thoroughly performed the CE of proteins ranged from 14.3 kDa to 116 kDa in a mixed hydroxyethyl cellulose (HEC) solution. The mixed solution was obtained with various Mrincluding 90,000, 250,000, 720,000, and 1,300,000. Then, we found that the mixed polymer provided a high resolution for small protein molecules while increasing the efficiency of large ones. Results demonstrated that the migration time decreased if HEC (1,300,000) was mixed with the lower Mrone, and the mixed solution (1,300,000/250,000) offered the highest resolution. The resolution was negatively correlated with the electric field strength. Finally, we have employed the optimal electrophoretic conditions to separate proteins in human tears, and it showed that lysozyme, lipocalin, and lactoferrin from human tears were successfully resolved in the mixed HEC. Such work indicates that CE has the potential to be developed as a tool for the diagnosis of xerophthalmia, meibomian gland dysfunction, or other eye diseases.Keywords:capillary electrophoresis;protein separation;hydroxyethyl cellulose;relative molecular mass;flamingo
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Environmental Science-Nano
·
2021
·Vol. 8
·期 9
·2430-2440
·
IF 8.131
Lingxiangyu Li,Ashfeen Ubaid Khan,Xiang Zhang,Xiaoting Qian,Yawei Wang
With the rapid development of nanotechnology, personal care products with silver nanoparticles (Ag-NPs) or zinc oxide nanoparticles (ZnO-NPs) are being widely used because of their superior antibacterial efficacies. Biological fluids would thus inevitably interact with Ag-NPs or ZnO-NPs. Human tear fluid is an important model that was ignored in past studies, restricting the comprehensive understanding of the stability and health risks of Ag-NPs and ZnO-NPs. Herein, two simulated tear fluid models were examined, allowing us to understand potential transformations that can affect the properties and toxicity of Ag-NPs and ZnO-NPs. Both Ag-NPs and ZnO-NPs exhibited high chemical stability in the simulated tear fluid containing proteins even after an incubation time of 168 h due to the formation of biocoronae on the particles, resulting in inferior antibacterial activities of Ag-NPs and ZnO-NPs to E. coli. Nevertheless, Ag-NPs and ZnO-NPs could gradually transform into AgCl and Zn5(CO3)2(OH)6 respectively in the simulated tear fluid without protein, wherein AgCl or Zn5(CO3)2(OH)6 accounted for 59.7% of silver or 91.7% of zinc through a selective dissolution method established in this study. The size of AgCl particles was far smaller than that of pristine Ag-NPs, whereas Zn5(CO3)2(OH)6 exhibited a needle-crossed morphology with a large size compared to pristine ZnO-NPs. An enhanced antibacterial efficacy was observed for the transformed Ag-NPs relative to that for pristine Ag-NPs, since high concentrations of dissolved silver were detected in the culture medium with the transformed Ag-NPs. Compared to pristine ZnO-NPs, in contrast, the transformed ZnO-NPs showed moderate antibacterial activity to E. coli. In total, the chemical stability of Ag-NPs and ZnO-NPs in the simulated tear fluid was highly affected by the protein, which would further influence their antibacterial efficacies.
研究领域:
环境科学
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纳米生物学
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毒理学
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Ruitao Zou,Suyan Shan,Libin Huang,Zhi Chen,Tom Lawson,Mimi Lin,Lu Yan,Yong Liu
The noninvasive and real-time detection of glucose sugar from tears is promising for the early diagnosis and treatment of chronic diseases such as diabetes. However, its realization is a big challenge. A suitable biosensor electrode that can closely fit the eye and be electrochemically sensitive is still unrealized. In this work, nitrogen-doped graphene (N-G) was used as an ophthalmic electrode in a high-performance intraocular biosensor. The use of N-G has been reported elsewhere before as it is highly electroactive and so has a particular use in biosensors. We hereby present a novel procedure for making carboxylated chitosan-functionalized nitrogen-containing graphene (GC–COOH) by using a one-step ball-milling process. This process does not use toxic chemicals, flammable gases, or a high temperature. It is thus particularly easy to perform. The fabricated nanomaterial had a high electroactivity and was easily assembled as a glucose biosensor by the immobilization of glucose oxidase. The thus constructed biosensor has a high sensitivity at 9.7 μA mM–1 cm–2, a broad linear range at 12 mM, and a good detection limit of 9.5 μM. It was able to maintain this activity after a month of storage. We also report the intraocular use of this constructed biosensor. The as-prepared GC–COOH was found to be highly biocompatible to ophthalmologic cells such as corneal epithelial and retinal pigment epithelium cells. No change in the intraocular pressure or the corneal structure was measured in a New Zealand white rabbit model. The as-assembled sensor was worn by the animals for more than 24 h without undue impact. This result confirmed the biosensor’s potential for intraocular application in the clinic. Its assembly into a useful sensor shown here has great potential to provide real-time monitoring of glucose levels in tear fluids of patients with high sugar levels.
研究领域:
生物医学工程
·
材料科学
·
分析化学
·
医疗器械
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Matter
·
2020
·
IF 0
Shiqi Guo,Kaijin Wu,Chengpan Li,Hao Wang,Zheng Sun,Dawei Xi,Sheng Zhang,Weiping Ding,Mona E. Zaghloul,Changning Wang,Fernando A. Castro,Dong Yang,Yunlong Zhao
Smart contact lenses attract extensive interests due to their capability of directly monitoring physiological and ambient information. However, previous demonstrations usually lacked efficient sensor modalities, facile fabrication process, mechanical stability, or biocompatibility. Here, we demonstrate a flexible approach for fabrication of multifunctional smart contact lenses with an ultrathin MoS 2 transistors-based serpentine mesh sensor system. The integrated sensor systems contain a photodetector for receiving optical information, a glucose sensor for monitoring glucose level directly from tear fluid, and a temperature sensor for diagnosing potential corneal disease. Unlike traditional sensors and circuit chips sandwiched in the lens substrate, this serpentine mesh sensor system can be directly mounted onto the lenses and maintain direct contact with tears, delivering high detection sensitivity, while being mechanically robust and not interfering with either blinking or vision. Furthermore, the in vitro cytotoxicity tests reveal good biocompatibility, thus holding promise as next-generation soft electronics for healthcare and medical applications.
研究领域:
生物医学工程
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柔性电子学
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可穿戴医疗设备
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眼科诊断
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生理监测
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纳米生物技术
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转化医学
✓ 已验证
DOI
PubMed
ADVANCED MATERIALS
·
2024
·
IF 27.4
Xing Lu,Xujiao Zhou,Bin Song,Hong Zhang,Mingrui Cheng,Xingyu Zhu,Yuqi Wu,Haoliang Shi,Binbin Chu,Jiaxu Hong,Houyu Wang,Yao He
Existing tear sensors are difficult to perform multiplexed assays due to the minute amounts of biomolecules in tearsand the tiny volume of tears. Herein, we leverage DNA tetrahedral frameworks (DTFs) modified on the wireless portable electrodes to effectively capture three dimensional (3D)-hybridization chain reaction (HCR) amplifiers for automatic and sensitive monitoring of multiple cytokines in human tears. The developed sensors allow the sensitive determination of various dry eye syndrome (DES)-associated cytokines in human tears with the limit of detection down to 0.1 pg/mL, consuming as little as 3 microliters of tear fluid. Double-blind testing of clinical DES samples using the developed sensor and commercial ELISA showed no significant difference between them. Compared with single-biomarker diagnosis, the diagnostic accuracy of this sensor based on multiple biomarkers has improved by approximately 16%. The developed system offers the potential for tear sensors to enable personalized and accurate diagnosis of various ocular diseases. This article is protected by copyright. All rights reserved
研究领域:
生物医学工程
·
分子生物学
·
分析化学
·
眼科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 6
·1-10
·
IF 5.833
Zhao Junhong,Cheng Jing,Sun Yudong,Liu Jiang,Chen Wen,Xu Yi,Yang Jiao,Li Yingchun
A novel photoelectrochemical (PEC) sensor based on “Z-scheme” TiO 2 @Au@CdS and molecularly imprinted polymer (MIP) was developed for the non-invasive detection of uric acid (UA). The “Z-scheme” material, consisting of an electron-transfer system (Au) and two isolated photochemical systems (CdS, TiO 2 ), was synthesized by chemical deposition method and it worked as a substrate for electro-polymerization of MIP. Due to the high photoelectric conversion efficiency provided by TiO 2 @Au@CdS and specific imprinting effect afforded by MIP, the sensor displayed desirable sensing performance with the merits of sensitivity, selectivity, repeatability, and stability. The linear range for UA detection is from 1 nM to 9 μM with the detection limit of 0.3 nM (S/ N = 3). Moreover, the assay was successfully utilized to measure UA in human tears and offered a reliable result. The incorporation of MIP and “Z-scheme” material into a PEC sensor system is expected to provide a promising strategy for detecting other small molecules. Graphical abstract
研究领域:
分析化学
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生物传感器
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材料科学
·
光化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·
IF 19
Zhongxing Chen,Zheng Li,Nana Tang,Yida Huang,Siheng Li,Wenjin Xu,Qing Wang,Xin Chen,Nan Zhao,Zhenhai Zeng,Yichen Xiao,Xinyi Chen,Jiawei Li,Xingtao Zhou,Guisheng Li,Mei Yang,Jinhai Huang
Dry eye disease (DED) is a widespread ophthalmic illness that inflicts massive economic and medical burdens on society. Although oxidative stress is closely associated with the onset of DED, barely any quantities of antioxidants are clinically available owing to low efficiency, toxicity, and poor bioavailability. Cerium-based nanozymes possess promising enzyme-mimetic activities in scavenging reactive oxygen species (ROS). Moreover, the ultra-small metal–organic frameworks (MOFs) have the potential for more efficient drug delivery, ROS elimination, and therapeutic efficacy. However, the development of ultra-small cerium-based MOFs (Ce-MOFs) is limited to a small minority due to challenges in the synthetic process and stability. By simply adjusting raw ingredients concentrations, three types of Ce-MOFs with distinct particle sizes are synthesized: Ce-MOF 1 (213 nm), Ce-MOF 2 (36 nm), and Ce-MOF 3 (2 nm). In comparison, the obtained ultra-small Ce-MOF 3 exhibited superior ROS scavenging and antioxidant capacity, decreased cytotoxicity, and excellent ocular penetration. Therapeutically, Ce-MOF 3 is demonstrated to be highly efficient in alleviating DED by suppressing oxidative stress and inflammation, boosting corneal epithelial repair, and facilitating tear secretion recovery. The novel ultra-small Ce-MOF 3 may serve as a promising alternative treatment for DED and other ROS-related disorders when combined with ocular biocompatibility.
研究领域:
纳米技术
·
材料科学
·
生物医学工程
·
药学
·
眼科
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jia Xu,Xiaoqin Tao,Xiaoxuan Liu,Li Yang
We develop a novel noninvasive wearable biosensor to simultaneously assay multiple key biomarkers in human tears. The sensor is uniquely designed as a convenient-to-use eye patch that can be easily and comfortably fit under eyes. Different sensing regions on the eye patch are modified with specific chromogenic reagents to selectively determine the hydrogen ion (pH), protein, ascorbic acid, and glucose in tears. The assay takes only one drop of tear (∼20 μL) and a 30 s response time. The resulting color signals from each sensing region on the eye patch are captured and analyzed using a smartphone, and a cloud model based on the internet of things concept is recommended, which can extend the application of the eye patch biosensors in point-of-care testing and health monitoring, especially during the pandemic. Tear detection of the human subjects is performed using the proposed method, and the results display good correlation to parallel measurements using assay kits. The results show the great potential and versatility of the proposed eye patch biosensor for noninvasive determination of diverse tear biomarkers, facilitating the widespread application of the tear-based noninvasive wearable biosensor in emerging personal health care and clinical monitoring fields.
研究领域:
生物医学工程
·
传感器
·
即时检测
·
医疗技术
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2023
·
IF 5.6
Qi Liang,Rongjie Guo,Jia-Ruei Tsao,Yun He,Chenchen Wang,Jiaxuan Jiang,Di Zhang,Taige Chen,Tingting Yue,Kai Hu
Dry eye disease (DED) is a multifactorial disease, and oxidative stress plays a crucial role in its pathogenesis. Recently, multiple studies have shown that upregulation of autophagy can protect the cornea from oxidative stress damage. The present study investigated the therapeutic effects of salidroside, the main component of Rhodiola crenulata , in both in vivo and in vitro dry eye models. The results showed that topical eye drop treatment with salidroside restored corneal epithelium damage, increased tear secretion, and reduced cornea inflammation in the DED mice. Salidroside activated autophagy through AMP-activated protein kinase (AMPK)-sirtuin-1 (Sirt1) signaling pathway, which promoted the nuclear translocation of nuclear factor erythroid-2-related factor 2 (Nrf2) and increased the expression of downstream antioxidant factors heme oxygenase-1 (HO-1) and NAD(P)H quinone dehydrogenase 1 (NQO1). This process restored antioxidant enzyme activity, reduced reactive oxygen species (ROS) accumulation, and alleviated oxidative stress. The application of autophagy inhibitor chloroquine and AMPK inhibitor Compound C reversed the therapeutic efficacy of salidroside, validating the above findings. In conclusion, our data suggest that salidroside is a promising candidate for DED treatment.
研究领域:
药理学
·
眼科
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Xiaohan Kong,Yizhen Jia,Han Wang,Rui Li,Chujie Li,Shihong Cheng,Tian Chen,Yang Mai,Yichu Nie,Yang Deng,Zhiyong Xie,Yang Liu
Ocular formulations should provide an effective antibiotic concentration at the site of infection to treat bacterial eye infections. However, tears and frequent blinking accelerate the drug clearance rate and limit drug residence time on the ocular surface. This study describes a biological adhesion reticulate structure (BNP/CA-PEG) consisting of antibiotic-loaded bioadhesion nanoparticles (BNP/CA), with an average 500–600 nm diameter, and eight-arm NH2-PEG-NH2 for local and extended ocular drug delivery. This retention-prolonging effect is a function of the Schiff base reaction between groups on the surface of BNP and amidogen on PEG. BNP/CA-PEG showed significantly higher adhesion properties and better treatment efficacy in an ocular rat model with conjunctivitis in comparison to non-adhesive nanoparticles, BNP, or free antibiotics. Both in vivo safety experiment and in vitro cytotoxicity test verified the biocompatibility and biosafety of the biological adhesion reticulate structure, indicating a promising translational prospect for further clinical use.
研究领域:
药学
·
眼科学
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）