ACTA PHYSIOLOGIAE PLANTARUM
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2024
·Vol. 46
·期 3
·1-11
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IF 2.4
Wu Wenqing,Li Simeng,Gu Wei,Tian Rong,Luo Man,Tang Junjie,Tang Min,Wu Yinzhi
Alismatis rhizoma is an essential medicine in clinical practice. Asian water plantain ( Alisma orientale (Sam.) Juzep) is one of the original plants of Alismatis rhizoma. Previous studies have identified the optimal light intensity range for the growth and development of A. orientale , but the mechanism by which light intensity affects the accumulation of secondary metabolites of A. orientale is unknown. The aim of this study was to investigate the effect of light intensity on the accumulation of triterpenoids in A. orientale saplings and its potential molecular mechanism. The dry weight and contents of total triterpenes and indicative components (alisol B 23-acetate and alisol C 23-acetate) as well as the expression of key enzyme genes in the triterpene biosynthesis pathway under different light intensities (50–600 μmol m −2 ·s −1 ) were determined. The results showed that the accumulation of dry matter and the contents of total triterpenes, alisol B 23-acetate, and alisol C 23-acetate increase first and then decrease with increasing light intensity, with the maximum values of 31.65 g, 18.35 mg·g −1 , 1.91 mg·g −1 , and 0.13 mg·g −1 recorded at light intensities of 400, 200, and both of 300 μmol m −2 ·s −1 , respectively. Light intensities of 200–400 μmol m −2 ·s −1 promote the expressions of key enzyme genes and the accumulation of total triterpenes significantly. Correlation analysis showed that the expression levels of key enzyme genes are significantly correlated with total triterpene and indicative component contents, and these correlations are strongest under moderate light intensities. Overall, our results reveal that a moderate light intensity of 200–400 μmol m −2 ·s −1 is beneficial for the growth and synthesis of protostane triterpenes in A. orientale seedlings, and that its probable mechanism involves the upregulated expression of enzymes that are key in the synthesis of triterpenoid ingredients. This study clarified the suitable light intensity range for the synthesis and accumulation of protostane triterpenes of A. orientale , which provided scientific basis for the production of high-quality superior forms of A. orientale .
研究领域:
生药学
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植物生理学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2023
·
IF 3
Laisen Wang,Liyue Hou,Siliang Han,Huaizhong Guo,Ligai Bai
A monolithic solid-phase extraction (SPE) cartridge packed with a composite adsorbent was fabricated via polymerization using dodecene as the monomer with the porous organic cage (POC) material doped, combing with an analytical column through a high-performance liquid chromatography (HPLC) instrument, which was used for the online extraction and separation of 23-acetyl alismol C, atractylodes lactone II and atractylodes lactone III from Zexie Decoction. The POC-doped adsorbent shows porous structure with a relatively high specific surface area of 85.50 m 2 /g, which was obtained from the characterizations of a scanning electron microscope and an automatic surface area and porosity analyser. Efficient extraction and separation of three target terpenoids was achieved by an online SPE-HPLC method based on the POC-doped cartridge, which exhibits strong matrix-removal ability and good terpenoids-retention ability with a high adsorption capacity, due to the interactions of hydrogen bond and hydrophobicity between the terpenoids and the POC-doped adsorbent. Method validation shows good linearity ( r ≥ 0.9998) of the regression equation, and high accuracy with the spiked recovery in the range of 99.2 %-100.8 % of the proposed method. Compared to the generally disposable adsorbent, this work fabricated a reusable monolithic cartridge, which can be used for at least 100 times, with the RSD based on the peak area of the three terpenoids less than 6.6 %.
研究领域:
分析化学
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药学
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中药学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
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2025
·
IF 5.1
Fenxia Han,Yongqiang Wang,Hao Zhang,Sheng Zhang
Introduction: Alisma starch (AS) from Alismatis Rhizoma has potential applications but has been less studied compared to common starches like corn starch (CS) and potato starch (PS).Methods: We used scanning electron microscopy, X-ray diffraction, and rapid visco analysis to study the granule morphology, crystal structure, pasting properties, freeze -thaw stability, solubility, swelling degree, and gel strength of AS, CS, and PS.Results: AS has a lower starch content but higher amylose content than CS and PS. It has a smaller particle size and is A-type starch. Its pasting temperature and trough viscosity are higher, and its freeze -thaw stability is intermediate. Gel strength increases with concentration and shows no significant difference between 10% AS and 12% PS.Discussion: AS has good heat resistance, shear resistance, and gel strength, indicating potential for high-temperature processed foods. Future research should focus on its heat resistance mechanism and broader applications.
研究领域:
食品科学
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植物学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Neurology
·
2025
·
IF 2.8
Renlong Ji,Yanchang Xu,Kang Li,Wen Jiang,Yuan Li,Jianwei Zeng,Wei Li,Caiji Wang,Zeqi Zhao,Yalan Li,Naveena Konduru,Wen Liu,Yuehua Qiao,Xuanyi Li
Objective: To investigate the effects of Zexie decoction on vestibular and auditory function in guinea pigs with endolymphatic hydrolysis induced by desmopressin. Methods: Sixty guinea pigs were randomly and evenly divided into four groups, each group has 15 guinea pigs: normal control group, DDAVP group, DDAVP modeling combined with Zexie Decoction group, and DDAVP combined with Double Zexie group. At 7 and 14 days, bone-conducted cervical vestibular evoked myogenic potential tests, auditory brainstem responses, and distortion-product otoacoustic emissions were conducted on each group of guinea pigs to evaluate their vestibular and auditory function quantitatively. After functional testing, the outer hair cells were observed by scanning electron microscope. On day 14, one guinea pig was randomly selected from both the normal control group and DDAVP group to verify the successful establishment of the model using gadolinium-enhanced magnetic resonance imaging of the inner ear.Results: We conducted BC-cVEMP, ABR, and DPOAE tests on guinea pigs, and the results showed that DDAVP did affect vestibular function and hearing in guinea pigs. Analyses were performed from those results that were statistically significant, Zexie Decoction improved DDAVP-induced vestibular dysfunction and hearing loss dose-dependently, though complete reversal was not achieved. About scanning electron microscopy, outer hair cells of the DDAVP group showed significant loss and cilia lodging, however, treatment with Zexie decoction can alleviate the loss of outer hair cells and the lodging of cilia. When the outer hair cells were exposed to DDAVP for a long time, the improvement effect of Zexie decoction was not as obvious as before.Conclusion: The extent of improvement correlates with the concentration and dosage of Zexie Decoction. Even at double the dosage, Zexie Decoction only partially mitigates the decline in vestibular and auditory function induced by DDAVP, falling short of complete reversal.
研究领域:
药理学
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听力学
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神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Analytical Methods in Chemistry
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2020
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IF 1.878
Fei Sun,Xiaolu Yang,Fei Liu,Ying Zhang,Shumei Wang,Hui Cao,Jiang Meng
In this study, high-performance liquid chromatography (HPLC) and colorimeter were applied to evaluate the quality of different species and differently prepared slices of Zedoray Rhizome samples with the aid of chemometric tools. Fifty batches of Zedoray Rhizome samples from different species and forty-two batches of Zedoray Rhizome samples from differently prepared slices were collected. The quantitative method was developed using HPLC to simultaneously determine the contents of twelve chemical ingredients in Zedoray Rhizome. The colour parameters L, a, and b were measured by a colorimeter. Then, the collected data were analyzed by the principal component analysis and Pearson correlation analysis. The results showed that the proposed method was capable of accurately determining the contents of the twelve chemical ingredients and the colour parameters for the collected samples. There was a dramatic difference in the contents of the chemical ingredients and in the colour parameters among different species and differently prepared slices of Zedoray Rhizome samples. This study reveals that combining HPLC, colorimeter, and chemometric tools can provide a new approach to comprehensively evaluate the quality of Zedoray Rhizome samples.
研究领域:
中药学
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分析化学
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植物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2022
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IF 5.195
Zhishen Xie,Er-wen Li,Gai Gao,Yueyue Du,Mengyao Wang,Hui Wang,Pan Wang,Yonghui Qiao,Yunfang Su,Jiangyan Xu,Xiaowei Zhang,Zhenqiang Zhang
Ethnopharmacological relevance Zexie Tang (ZXT), only two consists with Alismatis Rhizoma (AR) and Atractylodes macrocephala Rhizoma (AM), a classical Chinese medicine formula from Synopsis of the Golden Chamber with a history of 2000 years. Clinical observation in recent years has found that ZXT has excellent lipid-lowering effect. Aim of the study : To explore the potential mechanism of ZXT ameliorates hyperlipidemia based on FKBP38/mTOR/SREBPs pathway. Materials and methods WD-induced hyperlipidemia mice and oleic acid induced cell lipid accumulation model were used to investigate pharmacodynamic . The effect of ZXT on the transcriptional activity of SREBPs was detected by reporter gene assay. Proteins and downstream genes of mTOR/SREBPs pathway were detected in vivo and in vitro . Combined with network pharmacology and HPLC-Q-TOF/MS, the active ingredients were screened and identified. The interaction between active compounds of ZXT and FKBP38 protein were analyzed by docking analysis. Results ZXT decreased TC, TG and LDL-c levels in blood of WD-induced hyperlipidemia mouse model, and improved insulin resistance in vivo . ZXT also reduced TC, TG and lipid accumulation in cells line, and inhibited SREBPs luciferase activity, protein and its target genes expression such as FASN , HMGCR , etc. Meanwhile, ZXT inhibited protein expression levels of p-mTOR, p-S6K, etc in vitro and in vivo . Combined with network pharmacology and HPLC-Q-TOF/MS, 16 active ingredients were screened and identified. Docking results showed that active compounds of ZXT binding to FKBP38 and formed hydrogen bond . Conclusion Our findings highlighted that ZXT ameliorates hyperlipidemia, in which FKBP/mTOR/SREBPs pathway might be the potential regulatory mechanism.
研究领域:
药理学
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分子生物学
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中医药
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产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2024
·
IF 6.9
Yan Xie,Yimin Jin,Jianhui Wen,Guiping Li,Xue Huai,Yueyang Duan,Fuyong Ni,Juan Fu,Ming Li,Liang Li,Ming Yan,Liang Cao,Wei Xiao,Hao Yang,Zhen-zhong Wang
Non-alcoholic fatty liver disease (NAFLD), particularly advanced non-alcoholic steatohepatitis (NASH), leads to irreversible liver damage. This study investigated the therapeutic effects and potential mechanism of a novel extract from traditional Chinese medicine Alisma orientale (Sam.) Juzep (AE) on free fatty acid (FFA)-induced HepG2 cell model and high-fat diet (HFD) + carbon tetrachloride (CCl 4 )-induced mouse model of NASH. C57BL/6 J mice were fed a HFD for 10 weeks. Subsequently, the mice were injected with CCl 4 to induce NASH and simultaneously treated with AE at daily doses of 50, 100, and 200 mg/kg for 4 weeks. At the end of the treatment, animals were fasted for 12 h and then sacrificed. Blood samples and liver tissues were collected for analysis. Lipid profiles, oxidative stress, and histopathology were examined. Additionally, a polymerase chain reaction (PCR) array was used to predict the molecular targets and potential mechanisms involved, which were further validated in vivo and in vitro . The results demonstrated that AE reversed liver damage (plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatocyte ballooning, hepatic steatosis, and NAS score), the accumulation of hepatic lipids (TG and TC), and oxidative stress (MDA and GSH). PCR array analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that AE protects against NASH by regulating the adipocytokine signaling pathway and influencing nuclear receptors such as PPARα. Furthermore, AE increased the expression of peroxisome proliferator-activated receptor gamma coactivator-1α (PPARGC1α) and reversed the decreased expression of PPARα in NASH mice. Moreover, in HepG2 cells, AE reduced FFA-induced lipid accumulation and oxidative stress, which was dependent on PPARα up-regulation. Overall, our findings suggest that AE may serve as a potential therapeutic approach for NASH by inhibiting lipid accumulation and reducing oxidative stress specifically through the PPARα pathway.
研究领域:
药理学
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肝病学
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分子生物学
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传统中药
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Zhaoyi Miao,Yue Hu,Xiaojing Zhang,Xiaotong Yang,Yingying Tang,An Kang,Dong Zhu
Currently, nanosphere-based ligand fishing cannot be accomplished with imaging processing, although this step is important for real-time identification. Herein, a ligand fishing technique combined with real-time imaging is presented for the identification of ligands for heat shock protein 90α (Hsp 90α) from a complex matrix, Alisma plantago-aquatica Linn . crude extract, using Hsp 90α-functionalized mesoporous silica nanoparticle (MSN)-InP/ZnS quantum dot (QD) nanocomposites as a support material. Twenty ligands for Hsp 90α were screened, and their structures were identified by mass spectrometry. The activities of the ligands were verified by real-time imaging of cells apoptotic morphological changes. Quantitative analysis showed that Alisma plantago-aquatica Linn contained 8.19 μg/g Alisol F, which regarded as one typical component of Alisma plantago-aquatica Linn , and the extraction ratio of Alisol F was 76.2%. The precision for five replicate measurements was 7.0% (RSD). The prepared nanocomposites were also used to screen proteins from a mixture of cellular extracts, and five proteins from HeLa cells were identified as potential client proteins of Hsp 90α.
研究领域:
纳米技术
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生物化学
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药学
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分子生物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
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2021
·
IF 7.086
Huanping Liu,Xiaoyan Tang,Xiaomin Xu,Yunv Dai,Xiaomeng Zhang,Yang Yang
Broad-spectrum insecticides such as neonicotinoids tend to accumulate and detrimentally impact natural ecosystems. Accordingly, we aimed to assess the neonicotinoid phytoremediation abilities of nine wetland plant species commonly used in constructed wetland systems: Acorus calamus , Typha orientalis , Arundo donax , Thalia dealbata , Canna indica , Iris pseudacorus , Cyperus alternifolius , Cyperus papyrus and Juncus effusus. We assessed their removal of six neonicotinoids and explored the mechanisms responsible for the observed removal in a 28-day experiment. The planted systems effectively removed the neonicotinoids, with removal efficiencies of 9.5–99.9%. Compared with the other neonicotinoids, imidacloprid , thiacloprid and acetamiprid were most readily removed in the planted systems. C. alternifolius and C. papyrus exhibited the best removal performance for all six neonicotinoids. Based on our assessment of mass balance, the main removal processes were biodegradation and plant accumulation. Plants can enhance neonicotinoid removal through enhancing biodegradation. The differences in transport and accumulation behaviors may be related to plant species and physicochemical properties of neonicotinoids. Further research is merited on the toxicity of neonicotinoids to plants and microorganisms and the metabolic pathways by which neonicotinoids are broken down in wetland systems.
研究领域:
环境科学
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生态学
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植物修复
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湿地生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）