Desalination and Water Treatment
·
2024
·
IF 1
Binglu Deng,Xingqiu Zhou,Xingjian Yang,Zhi Dang,Guining Lu
ABSTRACT This study reports on the adsorption of polychlorinated biphenyls (PCBs) by powder activated carbon (PAC) in soil washing eluents containing tween-80 surfactant micelles. Factors influencing of adsorption were studied (i.e., time, adsorbents doses, molecular structure of different PCBs congeners, dynamic and thermodynamic models). The adsorption of PCBs on nano bamboo charcoal (NBC) was compared with those on PAC. Finally, the prospect for recycling soil eluents was explored. We found that the PAC and NBC were both effective in removal of 12 mg/L PCBs in soil washing eluents. The results of the adsorption kinetics showed that both materials have different adsorption rate controlling step, the particles diffusion became adsorption rate controlling step of NBC while particle diffusion and adsorption reaction like chemical bonding reactions co-control the adsorption rate of PAC. The adsorption of PCBs on PAC was probably chemisorption through interaction between aromatic rings and oxygen-containing functional groups while the adsorption on NBC was mainly physisorption by micropore filling. After the PAC treatment, the residual tween-80 in the eluents was 27.78% (total mass ratio). The addition of acetone to break micelles before adsorption could increase the recovery of tween-80 to 73.30% while PCBs removal rate could still reach 78.06%. Therefore, the surfactant solutions can be reused after adsorption. The studies provide valuable information on the adsorption of PCBs by PAC in soil washing eluents, which may improve the utilization and selection of PAC in the recovery of soil washing eluents.
研究领域:
环境科学
·
污染治理
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DOI
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Shuo Guo,Yuanqiang Sun,Jinquan Li,Xin Geng,Ran Yang,Xiaoge Zhang,Lingbo Qu,Zhaohui Li
Monitoring cell viability plays a vital role in fundamental aspects of pathology, medicine, and biology. Mitochondria and the nucleolus are involved in regulating cell viability in many biological processes, such as mitochondrial autophagy, apoptosis, and cell growth. Thus, exploring the variational cell viability reflecting with mitochondria and the nucleolus is very significant and highly required. Herein, in this work, a kind of fluorescent carbon dots (CDs) capable of shuttling between mitochondria and the nucleolus was synthesized through a simple microwave-assisted strategy and utilized for in situ visualization of cell viability. Meanwhile, hydrogen peroxide (H2O2)-induced cell damage and ascorbic acid-mediated cell protective effects were successfully visualized. Moreover, these CDs demonstrate an intrinsic character of wash-free imaging that makes them preferable for the monitoring of cell viability. We believe that the developed CDs have a great potential to be used as a robust tool to facilitate the progress in cell viability-related medicine, biology, and pathology studies.
研究领域:
细胞生物学
·
医学
·
病理学
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产品关联
DOI
SOFT MATERIALS
·
2018
·
IF 1.132
Liping Zhang,Ming Liu,Tian Qi,Haili Zhou,Caihua Ni
Embolization using microspheres plays an important role in interventional cancer therapy. The drug-eluting embolization microspheres were prepared via reverse suspension crosslinking of gelatin, followed by modification of the cross-linked microspheres. The microspheres displayed regular spherical shapes with size distribution ranging from 180 μm to 350 μm. The negatively charged groups (COO−) were introduced into the microspheres which could load positively charged doxorubicin with loading rates as high as 26.1% and 34.0% in 1 and 12 h, respectively. The high drug-loading rates were ascribed to ionic interaction between carboxyl groups and doxorubicin. The drug release profile showed the modified microspheres (MMS) have a sustained release manner compared with non-MMS in phosphate buffered saline (PBS). The MMS were almost completely degradated after 20 days. Cytotoxicity assay certified that the MMS were non-cytotoxic, and could be potentially used as biocompatible materials.
研究领域:
医学
·
药学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jie Zhou,Xuyi Zhang,Bicheng Deng,Yabin Huang,Wangqiang Kuang,Xiaojuan Liu,Shengting Kuang,Zhaomin Hao,Wuping Liao
Precise recognition and deep removal of trace thorium from rare earths remains a significant challenge. Herein, we prepared a polycinnamic acid (PCA) resin by emulsion polymerization to achieve efficient separation of thorium from rare earths. The PCA resin leverages its specific pore size, steric hindrance and relatively large electronegativity of thorium to precisely recognize trace thorium ions in rare earth solutions. For instance, a separation factor of 4158 was achieved for Th/Lu at a Lu/Th molar ratio of 350. The resin also exhibited an exceptional adsorption capacity for Th(IV), reaching 405.2 mg/g at 328 K, surpassing most of the previously reported adsorbents. The adsorption process was determined by a combination of intra-particle diffusion and monolayer chemisorption, driven by entropy in a spontaneous endothermic reaction. Desorption of thorium from the loaded resin ions was effectively accomplished using H 2 SO 4 solution at pH < 2. Additionally, the PCA resin exhibited excellent cycling and regeneration performance, maintaining stability under various acidic conditions and immersion durations. In practical applications, the PCA resin reduced the thorium concentration from 5.5 mg/L to 0.005 mg/L in simulated radioactive wastewater and from 0.56 mg/L to 0.007 mg/L in high-purity LuCl 3 solution, with no measurable loss of rare earths. Characterization through FTIR, Raman and XPS analysis revealed that two oxygen atoms of the –COOH group are bidentate chelating coordination with thorium ions. Furthermore, density functional theory (DFT) calculations provided insights into the selective binding mechanism at the –COOH site, highlighting its role in the effective separation of thorium from rare earths.
CAS / MDL:
研究领域:
化学工程
·
材料科学
·
环境科学
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产品关联
DOI
高影响因子
STAR Protocols
·
2022
·
IF 0
Yuzhu Cao,Lan Meng,Yu Cheng,Muhammad Azhar,Xue Jiang,Xuemei Xing,Jianqiang Bao
Summary Germline development is challenging to study due to the diversity of cell types in mammalian testis. Here, we present an optimized protocol, namely centrifugal elutriation, that allows the simultaneous isolation of mouse germ cells at different stages with high purity within ∼2 h. This approach exploits the JE-5.0 centrifugal elutriation system that fractionates cells based on differential sedimentation gravity. We herein provide the optimized parameters and procedures for isolation of elongating spermatids, round spermatids, and pachytene spermatocytes from adult mouse testes. For complete details on the use and execution of this protocol, please refer to Bao et al. (2018) .
研究领域:
生殖生物学
·
细胞生物学
·
发育生物学
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DOI
PubMed
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Tianpei Cai,Xinyue Lei,Hai-Bo Shang,Xia Li,Yetong Guo,Li-Xin Wu,Huwei Liu,Donghao Li
Generally, the traditional stationary phase for liquid chromatography is the key part, but with an in situ immutable property, leading to many separation limitations. Based on the former exploration of photosensitive gas chromatography, we successfully prepared a photosensitive monolithic capillary silica column with high light transmission, taking advantage of the reversible cis–trans isomerism of azobenzene. And the cis–trans isomerism has launched an effective, reversible, and precise control on the liquid chromatographic retention behavior just by photoinduction according to the theoretical basis of a good correlation between photoinduction time, trans-azobenzene ratio, and chromatographic retention factor (k) (R2 > 0.9586). In this system, the plausible control mechanism of stationary phase’s polarity is the synergistic effect of the self-molecular polarity difference and the occupation (or release) of polar groups on the stationary phase surface by cis–trans isomerism. Furthermore, a “light control–time ratio cycle” strategy was first reported to maintain an arbitrary ratio of trans-azobenzene corresponding to the polarity gradient of the stationary phase during the whole chromatographic separation process, which is verified by a close correlation of the retention time of the targets and the ratio of light-controlled time (R2 > 0.9977). The “gradient elution” of the targets was also surprisingly realized due to the gradient regulation of stationary phase’s polarity by the programmatic control of alternate light induction and time ratio. This work further advances the practical significance of photosensitive chromatography in “one column for multiple columns”, even “one column for multidimensional chromatography”, and truly provides research foundation for its development in the separation and analysis of complex biological samples.
研究领域:
分析化学
·
色谱技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Fuxin Zhang,Xingcui Wang,Xinyi Huang,Jinlong Zhang,Duolong Di,Dong Pei,Jun Hai
The fact that both phases are liquids makes it simple to implement counter-current chromatography (CCC), which involves alternating elution modes and phase roles during the separation process. Using two distinct multiple dual-mode (MDM) elution techniques for chiral separation, the team demonstrated a superior separation effect in a prior study. When selecting a chiral selector with high enantiomer recognition capability is difficult, the separation efficiency of the CCC column can be enhanced through multiple cycles. This approach facilitates the preparation of enantiomers that are challenging to isolate under classical CCC conditions. A straightforward mathematical model was created in this study to forecast the number of cycles and the mode conversion time needed for the separation process after the modified MDM method was further examined theoretically. After modeling molecules, the theoretical model was confirmed, and the outcomes generally agreed with the calculations from the theoretical model. This offers substantial support for the modified MDM method's real-world implementation in chiral separation.
研究领域:
化学
·
分析化学
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DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Gao Yuchen,Jiang Jianguo,Tian Sicong,Li Kaimin,Yan Feng,Liu Nuo,Yang Meng,Chen Xuejing
Soil washing is an effective remediation method to remove heavy metals from contaminated soil. However, it produces wastewater that contains large amounts of heavy metals, which lead to serious pollution. This study investigated the removal of vanadium (V) from synthetic soil washing effluent using BOF steel slag. The effects of particle size, slag dosage, initial pH, and initial vanadium concentration on removal behavior were studied. Adsorption kinetics and isotherms were also analyzed. The results showed that the vanadium removal efficiency increased as the steel slag particle size decreased and as the amount of slag increased. The initial pH and vanadium concentration did not play key roles. At the optimum particle size (<0.15 mm) and dosage (50 g/L), the removal rate reached 97.1% when treating 100 mg/L of vanadium. The influence of the washing reagent residue was studied to simulate real conditions. Citric acid, tartaric acid, and Na 2 EDTA all decreased the removal rate. While oxalic acid did not have negative effects on vanadium removal at concentrations of 0.05–0.2 mol/L, which was proved by experiments using real washing effluents. Considering both soil washing effect and effluent treatment, oxalic acid of 0.2 mol/L is recommended as soil washing reagent.
研究领域:
环境科学
·
土壤修复
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 28
·19795-19805
·
IF 3.9
Longyan Song,Minxia Liu,Min Nian,Gang Yang
Manganese-based lithium-ion sieves have become some of the promising adsorbents for extracting Li+ from brines. However, manganese dissolution loss (MDL) severely impairs the stability and cyclicity of ion sieves. A novel ozone eluent was first developed to extract Li+ from lithium manganese oxides, which decreased MDL decreased from 5.89% to 0.11%, and after ten regeneration cycles, the adsorption capacity retained 85.39% of the initial value, which was better than 55.15% when only hydrochloric acid (HCl) was used as the eluent. Based on these phenomena, the mechanism for the O3 lowering of MDL was investigated. First, the catalytic decomposition reaction of O3 competed with the disproportionation reaction, and the involvement of O3 inhibited the occurrence of the disproportionation reaction. Additionally, the presence of O3 and reactive oxygen species provided a preferential electron acceptor compared to Mn3+ during the migration of electrons from the bulk phase to the surface. In this study, MDL was greatly reduced with a very simple strategy, and the cycling stability of the adsorbent was improved.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Xiaoning Feng,Zhiheng Li,Xiujie Yang,Bin Lou,Fushan Wen,Nan Shi,Wei Zhu,Shuhai Guo,Meixia Gu,Dong Liu
A large amount of oily soil (OS) is inevitably generated in the oil exploration and development process, which leads to serious environmental issues . However, the purification of OS remained a great challenge due to the unclear mechanism. In this work, the desorption behavior of OS with different particle sizes was investigated by microemulsion (ME) elution based on the accumulation effect and binding interaction. The oil removal efficiency ( R e ) of OS has been studied in four elution systems. The result confirmed that LAS-M can better remove oil contaminants. In addition, the accumulation effect, the distribution coefficient ( K D ) varied with the solid concentration ( C p ), was elaborated using the SCA-Langmuir model in detail. The results indicated that small particle-size OS has a more significant accumulation effect, which facilitated the desorption of oil molecules. Furthermore, XRD and XRF were employed to detect the soil mineral composition. The FT-IR and zeta potential analysis revealed that the rich feldspar mineral in larger particle-size soil enhanced oil-soil binding interaction, directly leading to a decreased oil desorption capability. Finally, accumulation effect and binding effect revealed that small particle OS (∼41 µm, 89.17%) had higher R e than large particle OS (∼643 µm, 74.23%). The study provided a theoretical basis for the remediation of industrially contaminated soil.
研究领域:
环境科学
·
土壤学
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产品关联
DOI
高影响因子
Small
·
2023
·Vol. 20
·期 11
·2306638
·
IF 13.3
Yuheng Lv,Qin Fu,Yi Xu,Peng Shi
The achievement of rapid multiplexed protein imaging is limited by the use of stimulating reagents, extensive incubating and washing steps, and the low fluorescence intensity of targets. In this study, sequentially-activated DNA tags are developed and combined them with primary antibodies using signal enhancement strategies to create sequentially-activated antibodies (SAAs). These SAAs enable rapid, wash-free sequential imaging of different protein targets. The samples are pre-processed to label all targets of interest with SAAs simultaneously, and the signal is turned ON for only one target in each stage. The sequential imaging of multiple targets is achieved through wash-free strand displacement reactions that exhibit rapid kinetics with t 1/2 < 10 s in a cellular context. Remarkably, this method successfully demonstrates sequential imaging of nine different protein targets within just a few minutes. This all-in-one platform for multiplexed protein imaging holds great promise for diverse applications in immunofluorescence imaging.
研究领域:
生物化学
·
分子生物学
·
免疫学
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DOI
高影响因子
Pharmaceutics
·
2024
·Vol. 16
·期 12
·1609
·
IF 4.9
Xiaoli Zhang,Tingting Li,Jindong Tong,Meihong Zhou,Zi Wang,Xingdang Liu,Wei Lu,Jingjing Lou,Qingtong Yi
Background/Objectives: The purpose of this study was to develop the gemcitabine-loaded drug-eluting beads (G-DEBs) for transarterial chemoembolization (TACE) in rabbit renal tumors and to evaluate their antitumor effect using 2-deoxy-2-[(18)F]fluoro-D-glucose positron emission tomography/X-ray computed tomography (18F-FDG PET/CT). Methods: DEBs were prepared by polyvinyl alcohol-based macromer crosslinked withN-acryl tyrosine andN,N′-methylenebis(acrylamide). Gemcitabine was loaded through ion change to obtain G-DEBs. Their particle size and drug release profile were characterized. VX2 tumors were implanted in the right kidney of rabbits to establish the renal tumor model. The tumor-bearing rabbits received pre-scan by18F-FDG PET/CT, followed by targeted transarterial injection of G-DEBs under digital subtraction angiography (DSA) guidance. The rabbits received another18F-FDG PET/CT scan 10 or 14 days after the treatment. The therapeutic effect was further validated by histopathological analysis of the dissected tumors. Results: The average particle size of the microspheres was 58.06 ± 0.50 µm, and the polydisperse index was 0.26 ± 0.002. The maximum loading rate of G-DEBs was 18.09 ± 0.35%, with almost 100% encapsulation efficiency. Within 24 h, GEM was eluted from G-DEBs rapidly and completely, and more than 20% was released in different media. DSA illustrated that G-DEBs were delivered to rabbit renal tumors. Compared with the untreated control group with increased tumor volume and intense18F -FDG uptake, the G-DEBs group showed significant reductions in tumor volume and maximum standard uptake value (SUVmax) 10 or 14 days after the treatment. Histopathological analysis confirmed that the proliferating area of tumor cells was significantly reduced in the G-DEBs group. Conclusions: Our results demonstrated that G-DEBs are effective in TACE treatment of rabbit VX2 renal tumors, and18F-FDG PET/CT provides a non-invasive imaging modality to monitor the antitumor effects of TACE in renal tumors.
研究领域:
肿瘤学
·
影像医学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）