TISSUE & CELL
·
2021
·
IF 2.466
Limao Xu,Chengmin Liu,Zhiyao Ye,Chengfeng Wu,Yuhang Ding,Juan Huang
Long non-coding RNA (lncRNA) LINC00467 plays a proto-oncogenic role in non-small cell lung cancer. However, its effect and modulatory mechanism in gastric cancer (GC) are unknown. Thereby, we elucidated the mechanism of LINC00467 in GC. LINC00467 level in GC tissues was assessed by bioinformatic analysis, and clinicopathological parameters from GC patients were collected. The levels of LINC00467, integrin subunit beta 3 (ITGB3), proliferating cell nuclear antigen (PCNA), cleaved caspase-3 and cleaved poly (ADP-ribose) polymerase 1 (PARP1) in tissue samples or treated GC cells were assessed by quantitative real-time polymerase chain reaction (qRT-PCR), fluorescence in situ hybridization (FISH), or Western blot. The viability, proliferation and apoptosis of GC cells were detected by methyl thiazolyl tetrazolium assay, colony formation assay, and flow cytometry. Levels of LINC00467 and ITGB3 were up-regulated in GC, and highly expressed LINC00467 was positively associated with tumor size, differentiation, N stage, and T stage in GC patients. LINC00467 was enriched in cytoplasm of GC cells, and overexpressed LINC00467 promoted the viability and proliferation as well as levels of ITGB3 and PCNA, while suppressing the apoptosis and levels of cleaved caspase-3 and cleaved PARP1 in GC cells. Besides, the effects of shLINC00467 on inhibiting cell viability, proliferation of GC cells and PCNA level and promoting apoptosis as well as levels of cleaved caspase-3 and cleaved PARP1 were all partially reversed by overexpressed ITGB3. Overexpressed LINC00467 enhanced the viability and proliferation but inhibited apoptosis of GC cells via increasing ITGB3 level.
研究领域:
肿瘤学
·
分子生物学
·
癌症研究
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2016
·Vol. 16
·期 6
·5740-5745
·
IF 1.338
Deng Jiangming,Mo Yunfei,Liu Jianghui,Guo Rui,Zhang Yi,Xue Wei,Zhang Yuanming
Inorganic nanoparticles have been widely used in biomedical field. In this paper, we try to study the use of three types of inorganic nanoparticles (i.e., SnS2, BiOCl and SnS2-incorporated BiOCl (SnS2/BiOCl)) as doxorubicin (DOX) carriers. Firstly, SnS2,
BiOCl and SnS2/BiOCl were synthesised, then were characterized by TEM, nanoparticles size and zeta potential. Next the drug release and cell viability test were carried out. The cell viability test indicated that the drug carriers can effectively kill HeLa cells while maintaining
low cytotoxicity against normal cells-fibroblasts. The results show the potential of SnS2/BiOCl nanoparticles for antitumor applications.
研究领域:
生物医学
·
药物递送
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2017
·Vol. 17
·期 1
·1-16
·
IF 3.964
Huang Yutao,Lin Cheng,He Fei,Li Zhan,Guan Yajing,Hu Qijuan,Hu Jin
Background The low seed vigor and poor field emergence are main factors that restricting the extension of sweet corn in China. Spermidine (Spd) plays an important role in plant growth and development, but little is known about the effect of Spd on sweet corn seed germination. Therefore the effect of exogenous Spd on seed germination and physiological and biochemical changes during seed imbibition of Xiantian No.5 were investigated in this study.Results Spd soaking treatment not only improved seed germination percentage but also significantly enhanced seed vigor which was indicated by higher germination index, vigor index, shoot heights and dry weights of shoot and root compared with the control; while exogenous CHA, the biosynthesis inhibitor of Spd, significantly inhibited seed germination and declined seed vigor. Spd application significantly increased endogenous Spd, gibberellins and ethylene contents and simultaneously reduced ABA concentration in embryos during seed imbibition. In addition, the effects of exogenous Spd on H2O2 and MDA productions were also analyzed. Enhanced H2O2 concentration was observed in Spd-treated seed embryo, while no significant difference of MDA level in seed embryo was observed between Spd treatment and control. However, the lower H2O2 and significantly higher MDA contents than control were detected in CHA-treated seed embryos.Conclusion sThe results suggested that Spd contributing to fast seed germination and high seed vigor of sweet corn might be closely related with the metabolism of hormones including gibberellins, ABA and ethylene, and with the increase of H2O2 in the radical produced partly from Spd oxidation. In addition, Spd might play an important role in cell membrane integrity maintaining.
研究领域:
植物生理学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2024
·Vol. 149
·期 24
·5784-5790
·
IF 3.6
Yi-Xuan Li,Yu-Xuan Dai,Ju-Zheng Wang,Jérome Chauvin,Xue-Ji Zhang,Serge Cosnier,Robert S. Marks,Dan Shan
The concentration of zinc ions in semen is significantly correlated with sperm viability and male fertility. In this work, a reliable ratiometric fluorescence probe (ZIF-9-TCPP) based on the efficient Förster resonance energy transfer (FRET) process between two luminophores, benzimidazole (BIM) and meso-tetra (4-carboxyphenyl) porphyrin (TCPP) for Zn2+ detection has been constructed, where the emissions of BIM and TCPP are used as reference and detection signals. The proximity of BIM and TCPP in one framework (ZIF-9-TCPP) and the overlapped spectra between BIM and TCPP afford the attainment of a highly efficient FRET (around 90% efficiency). Efficient FRET improves the fluorescence intensity of porphyrin to enhance the sensitivity of detection. The unique spectral shift resulting from Zn2+ binding to the porphyrin ring ensures the selectivity of detection. In addition, the response mechanism of the proposed ratiometric probes to Zn2+ has been investigated. This work provides a convenient way to design an efficient FRET system and a promising method for sperm mobility detection.
研究领域:
生物化学
·
生殖医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Qunchao Chen,Lang Bai,Guoyang Wan,Yuefeng Hao,Xing Yang,Hongtao Zhang
Particulated juvenile articular cartilage (PJAC) has emerged as a promising living material for articular defect treatment. However, the fragile nature of PJAC hinders its wide clinical application. Here, inspired by the chemical composition and hierarchical structure of natural cartilage, we developed a novel hydrogel carrier system for PJAC delivery. Our carrier system, MeHA@J@DM, utilized methacrylated hyaluronic acid (MeHA) to incorporate PJAC and coated it with a polymerized mixture of dopamine methacrylamide (DMA) and 2-methylacryloyloxyethyl phosphorylcholine (MPC), forming an adhesive lubricant, p(DMA-MPC). MeHA@J@DM exhibited excellent performance for PJAC protection with enhanced cell viability, bioactivity, and lubrication properties. We evaluated the effectiveness of MeHA@J@DM in cartilage cell migration, where juvenile cartilage showed greater efficiency and remodeling abilities. In vivo rabbit cartilage defect models demonstrated superior cartilage regeneration with the MeHA@J@DM hydrogel. Our findings suggest that MeHA@J@DM has translational potential for PJAC implantation to enhance cartilage regeneration and benefit patients with articular cartilage lesions.
研究领域:
生物材料学
·
组织工程
·
再生医学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Shuo Guo,Yuanqiang Sun,Jinquan Li,Xin Geng,Ran Yang,Xiaoge Zhang,Lingbo Qu,Zhaohui Li
Monitoring cell viability plays a vital role in fundamental aspects of pathology, medicine, and biology. Mitochondria and the nucleolus are involved in regulating cell viability in many biological processes, such as mitochondrial autophagy, apoptosis, and cell growth. Thus, exploring the variational cell viability reflecting with mitochondria and the nucleolus is very significant and highly required. Herein, in this work, a kind of fluorescent carbon dots (CDs) capable of shuttling between mitochondria and the nucleolus was synthesized through a simple microwave-assisted strategy and utilized for in situ visualization of cell viability. Meanwhile, hydrogen peroxide (H2O2)-induced cell damage and ascorbic acid-mediated cell protective effects were successfully visualized. Moreover, these CDs demonstrate an intrinsic character of wash-free imaging that makes them preferable for the monitoring of cell viability. We believe that the developed CDs have a great potential to be used as a robust tool to facilitate the progress in cell viability-related medicine, biology, and pathology studies.
研究领域:
细胞生物学
·
医学
·
病理学
✓ 已验证
产品关联
DOI
Food & Function
·
2018
·Vol. 9
·期 12
·6227-6235
·
IF 3.289
Xunyu Song,Haitao Wang,Run Zhang,Chenxu Yu,Mingqian Tan
The potential health risks of food-borne nanoparticles are of great concern to public health. In this work, the formation and physicochemical properties of a class of fluorescent nanodots (FNDs) in roasted chicken were investigated. It was found that as the roasting temperature increased from 200 to 300 °C the size of the FNDs decreased significantly, from 17.1 to 2.1 nm. The FNDs, which mainly contain carbon (65%), are strongly fluorescent under ultraviolet light, exhibiting an excitation-dependent emission behavior. The fluorescence quantum yields of the FNDs were determined at 200, 250, and 300 °C, and were found to be 6.71 ± 0.21, 12.85 ± 0.34 and 17.46 ± 0.42%, respectively. These FNDs not only could be taken up by live cells and dispersed within the cytoplasm, but also could pass the blood–brain barrier (BBB) and accumulated in the brains of mice after oral feeding. The results of the fluorescence and Fourier transform infrared (FTIR) spectroscopy analysis clearly indicated that the FNDs can interact with the neurotransmitter dopamine in vitro through the formation of fluorescent conjugates. The relative cell viability decreased by 33% corresponding to FND-300 at a concentration of 4 mg mL−1. These results confirmed the presence of the FNDs in roasted chicken and revealed their potential bioeffects on human health, which calls for more research and evaluation.
研究领域:
食品科学
·
生物医学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACTA PHYSIOLOGIAE PLANTARUM
·
2010
·Vol. 33
·期 4
·1271-1276
·
IF 1.232
Guan Yajing,Hu Jin,Li Yongping,Ma Wenguang,Zheng Yunye
Counterfeit seeds cause a large loss in seed industry and crop production. Present study was undertaken to find a new anti-counterfeiting technology by labeling seeds with a fluorescent compound, safranine T (ST). Response surface analysis (RSA) experiment with two factors (ST concentration and seed soaking time) of 14 combinations was performed to investigate the ST effects on seed vigor and fluorescence showing in seedlings of two tobacco varieties, MS Yunyan 85 and Honghua Dajinyuan. After soaking in ST solutions, tobacco seeds were germinated for 16 days, then the germination index (GI), vigor index (VI), length of roots and shoots were determined. The optimum combinations of ST concentration and soaking time on GI, VI and length of shoots and roots were 0.57–0.69 mg/ml and 6.77–9.34 h for MS Yunyan 85 and 0.51–0.80 mg/ml and 5.81–7.39 h for Honghua Dajinyuan, respectively. Meanwhile, tobacco seedlings, treated with ST solutions from 0.3 to 1.5 mg/ml showed obviously fluorescence under illumination of green light. It suggests that soaking tobacco seeds in safranine T solution of suitable concentration can be used as a labeling technique for anti-counterfeiting in tobacco seeds.
研究领域:
种子科学
·
农业生物技术
·
植物生理学
·
分析化学
·
作物生产
✓ 已验证
DOI
高影响因子
JOURNAL OF DENTISTRY
·
2024
·
IF 4.8
XuanMei Ai,ZhaoNan Liu,TianQi Wang,Qi Xie,WeiLi Xie
Introduction This study create a dental composite by hybirding polyhedral oligo-sesquioxide nano monomers and bioactive glass BG 45S5. Methods Make an experimental composite resin material with a 60 % filler content overall by substituting 20 % of the filler with BG 45S5. The experimental resins are grouped and named P0, P2, P4, P6 and P8 based on the reactive nanomonomer methacrylic acid-based multifaceted oligomeric sesquisiloxane (POSS) added by 2 %–8 % in the resin matrix portion of each group. Utilize a universal testing machine to analyze and compare the mechanical properties of these, then perform Fourier infrared spectrum analysis, double bond conversion analysis, and scanning electron microscope analysis. Based on this, after soaking the experimental materials artificial saliva solution or lactic acid solution for a while, the pH changes of the solution, the release of Ca 2+ and PO 4 3− ions, and the precipitation of apatite on the resin material's surface were tested and analyzed. Cell viability tests were used to assess sample cell viability and quantify the cytotoxicity of biological cells. The independent sample t-test was used to examine the group comparisons, and a difference was considered statistically significant at P <0.05. Results Outstanding mechanical and the double bond conversion are demonstrated by the nanocomposites when the POSS concentration hits 4 wt%. Agglomeration will cause the performance to deteriorate if the concentration beyond this threshold. In the P4 group, the double bond conversion, CS, and FS rose by a large margin, respectively, in comparison to the blank control group P0. Thankfully, the data demonstrate that adding POSS increases adhesive ability when compared to the blank group P0, however, there is no discernible difference between the other experimental groups. The acid neutralization capacity of the P4 group is essentially the same as that of the control group (P0). Ca 2+ and PO 4 3− ions are released in significant amounts following treatment with lactic acid solution, although this tendency is clearly less pronounced in artificial saliva. SEM and EDX data indicate that when the experimental resin is soaked in lactic acid solution and artificial saliva, apatite precipitation will happen on its surface. The results of the cell viability test indicated that there was no statistically significant difference between the experimental groups, and the viability of the cells increased after 24hours and 48 hours. Conclusions POSS was included into the composite resin along with 20% bioactive glass as a filler. When the proportion of POSS is less than 4%, the indices of composite resin materials rise in a dose-dependent way. When this value is surpassed, performance begins to deteriorate. The inclusion of POSS has no influence on the biological activity of the composites, which means that the hybrid composite resin is capable of acid neutralization, ion release, and apatite precipitation. Clinical Significance The experimental composite resin can be used as an intelligent material in clinical treatment. It has the clinical application potential of preventing demineralization of tooth hard tissue, promoting remineralization, and improving edge sealing through apatite precipitation.
研究领域:
牙科材料学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
MEDICAL SCIENCE MONITOR
·
2020
·
IF 1.918
Shudong Chen,Chenglei Ren,Hui Zheng,Xianchun Sun,Jundi Dai
Background lncRNA HCP5 plays a cancer-promoting role in a variety of cancers. This study was the first to explore the mechanism of HCP5 in gastric carcinoma (GC). Material/Methods The differences in HCP5 between GC patients and healthy people were revealed in the TCGA database. The expression of HCP5 in GC tissues and adjacent tissues was compared by qRT-PCR. At the same time, the clinic pathological features of the patients were counted. Starbase and luciferase assay predicted and verified that miR-27b-3p is a targeted miRNA for HCP5. The expression of HCP5 and miR-27b-3p in various GC cells was detected by qRT-PCR. Cell viability and metastasis in different treatment groups were assessed by use of Cell Couting Kit-8 assay and clone formation assay, wound-healing assay, and transwell assay. Finally, expression of epithelial-mesenchymal transition (EMT)-associated markers was detected by Western blot. Results We found that HCP5 was overexpressed in GC tissues. Patients with higher expression of HCP5 had larger tumors, were more likely to have lymph node metastasis, and had higher TNM stage. HCP5 was overexpressed in GC cells, but this was reversed by miR-27b-3p. Silencing HCP5 inhibited GC cell viability and metastasis by downregulating Vimentin and N-cadherin and up-regulating E-cadherin, but this effect was partially reversed by miR-27b-3p inhibitor. Conclusions The effect of silencing HCP5 on repressing GC cells viability and metastasis by regulating EMT-associated markers can be partially reversed by miR-27b-3p inhibitor.
研究领域:
肿瘤学
·
分子生物学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 13
·10164-10169
·
IF 4.6
Shuai Han,Yunhan Qin,Dandan Zhang,Cuifang Li,Xiaoxue Du,Lijuan Liu,Songbo Yuan,Fangfang Wei,Baoxin Yang,Zhiying Ma
Red-emissive carbon dots (RCDs) were fabricated using a simple solvothermal method with Nile blue A sulphate and malic acid. The obtained RCDs exhibited good water solubility, excellent stability and a high luminescent quantum yield. The effect of RCDs on the growth of cotton sprouts was further explored, and it was found that the RCDs could effectively promote growth by enhancing root vitality. Furthermore, it was found that adding lanthanum to the RCD solutions could achieve better promotion, with the optimum concentration measured to be 0.02 mg mL−1 of RCDs for mixed solutions, which was much lower than that of the RCD solution alone (0.03 mg mL−1). Further analysis results showed that adding lanthanum could enhance the absorption of RCDs by the cotton sprouts, which might be induced by the enhancement of endocytosis in root cells triggered by lanthanum. This work not only provides novel long-wavelength emissive carbon dots with excellent biological activity but also holds positive significance for plant physiology and agricultural production.
研究领域:
植物学
·
农业科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Jingjing Chang,Peiling Wei,Meng Tian,Ying Zou,Shenghua Zhang
Screening for sensitive toxicological indicators and understanding algal tolerance to pharmaceutical contaminants (PhCs) are essential for assessing PhCs risk and their removal by microalgae . Carbamazepine (CBZ) showed adverse effects on microalgae , but the specific toxicity mechanisms on the most sensitive algal photosynthetic system (PS) remain limited. This study delved into the impact of CBZ exposure on the growth, cell viability , pigment content, and PS of Chlorella vulgaris . The findings revealed a notable inhibition of C. vulgaris growth by CBZ, with an IC 50 value of 27.2 mg/L at 96 h. CBZ exposure induced algal membrane damage and cell viability . Intriguingly, CBZ drastically diminished intracellular pigment levels, notably showing "low promotion and high inhibition" of chlorophyll b (Chl b) by 72 h. Moreover, the study identified a decreased number of active reaction centers (RCs) within algal PSII alongside inhibited electron transport from Q A to Q B on the PSII receptor side, leading to PSII disruption. As an adaptive response to CBZ stress, C. vulgaris stimulated its Chl b synthesis, increased non-photochemical quenching (NPQ), and adapted its tolerance to bright light. Additionally, the alga attempted to compensate for the CBZ-induced reduction in electron transfer efficiency at the PSII receptor side and light energy utilization by increasing its electron transfer from downstream. Principal component analysis (PCA) further verified that the parameters on non-photochemical dissipation, electron transport, and integrative performance were the most sensitive algal toxicological indicators for CBZ exposure, and algal PS has energy protection capability through negative feedback regulation. However, prolonged exposure to high doses of CBZ will eventually result in permanent damage to the algal PS. Hence, attention should be paid to the concentration of CBZ in the effluent and the exposure time, while methods to mitigate algal photodamage should be appropriately sought for algal treatment of dense effluents.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）