Advanced Biology
·
2025
·
IF 2.6
Xuewei Zhang,Zhihao Fang,Boon Chin Heng,Xiaoyu Hu,Zigang Ge
Cartilage regeneration in juvenile mice was better than in adult mice. This study evaluated the roles of cytokines and reactive oxygen species (ROS) after cartilage injury in both juvenile and adult mice and attempted to correlate these with cartilage progenitor cells and age-related differences in cartilage regeneration. Full-thickness cartilage defects were created in the femoral trochlea of knee joints in both 4-week-old (juvenile) and 8-week-old (adult) mice. Adult mice showed higher ROS peaks than juveniles at day 7 post-injury. Protein expression levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) significantly decreased after surgery, while interleukin-1β (IL-1β) levels displayed no significant change. There were more cartilage progenitor cells together with more cell proliferation in juvenile versus adult mice, while there was much less apoptosis of cartilage progenitor cells in juvenile mice compared to adult mice. ROS inhibition enhanced cartilage regeneration in adult mice by promoting progenitor cell proliferation and reducing apoptosis, mimicking the regenerative pattern seen in juveniles. This study demonstrated that inhibiting ROS in adult mice promoted cartilage regeneration, possibly by enhancing proliferation and decreasing apoptosis of cartilage progenitor cells.
研究领域:
医学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Qing Li,Run Lu,Huiyu Jiang,Yuyang Zhou
Establishing an energy/water-saving bleaching process is highly expected by textile plants to reduce industrial carbon emission, wastewater discharge and general cost. Therefore, an efficient UVA-assisted N-[4-(triethylammoniomethyl)-benzoyl]- caprolactam-chloride (TBCC) -activated hydrogen peroxide (H 2 O 2 ) bleaching strategy at ambient temperature for cotton/spandex (C/S) fabric was developed in this study. Results reveal that 7.4 g/L of TBCC-activated H 2 O 2 (30%) achieves a slightly higher whiteness index (WI) on C/S fabrics than those only treated with 60 g/L H 2 O 2 (30%). The treated C/S fabric displays a good dyeability using direct dyes due to the qualified wettability. The mild pH process protects C/S fabrics from damage with a low tensile loss (less than 10%) in warp and weft directions. The WI of C/S fabric mainly depends on the concentration of TBCC/H 2 O 2 couple and radiation time. Moreover, the degradation mechanism of cotton pigment in UVA/TBCC/H 2 O 2 bleaching system was implemented including the identification of effective reactive oxygen species (ROS), tracking of the concentration variation of hydroxyl radical (HO•) under UVA irradiation, and the exploration of degrading kinetics and pathway of morin as the model of pigment impurity. Indicated by the degradation rate constant, both TBCC and UVA plays an accelerating role in enhancing the degradation efficiency. In the radical scavenging experiment, HO• and superoxide radical (O 2 – •) are two major ROS participating in the degradation. And therein, HO• makes larger contribution than O 2 – •. HO• concentration has a high positive correlation with radiation duration and TBCC dosage based on the fluorescence labelling method. Confirmed by LC-MS/MS analyses, 2,4,6-trihydroxybenzoic acid (MW: 170.12) and 2,4,-dihydroxybenzoic acid (MW: 154.12) are two major segments degraded from the oxidation and ring-opening of C-ring in morin. Finally, considering energy, water and chemical conservations, the condition i.e. 7.4 g/L of H 2 O 2 (30%) and 20 g/L of TBCC under 30 W UVA radiation for 1 h, is recommended, which significantly increases the WI by 46.5%, and saves about 90% energy and 50% water consumption compared with the conventional waterbath bleaching at 95 °C for 45 min when similar WI enhancement was achieved.
研究领域:
纺织科学与工程
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Jinze Wang,Haiqi Zhang,Sentao Hu,Lie Ma
Excessive reactive oxygen species (ROS) leads to persistent inflammation in diabetic wounds inducing excessive inflammatory factors and matrix metalloproteinases (MMPs) and thereby hindering wound healing. However, low concentration ROS also function as a signaling molecule for maintaining cell function and promoting vascularization. Therefore, it is important to regulate ROS levels adaptively to match the healing process. Here, a nanozyme hydrogel was developed to intelligently clear wound ROS in response to dynamic changes of matrix metalloproteinase-9 (MMP9). Specifically, Prussian blue nanoparticles (PBNPs) loaded-gelatin nanospheres (PGs) were encapsulated in polyvinyl alcohol (PVA) hydrogel to obtain the nanozyme hydrogel (PGs@PVA). Hydrogen bonding between PGs and PVA not only improves the mechanical properties of PGs@PVA hydrogel but also ensures the controlled release of PBNPs. With the release of PBNP from PGs@PVA hydrogel in response to the high level of MMP9 in the pro-inflammatory stage, excessive ROS were cleared. The phenotype of macrophages was regulated correspondingly. The in vivo results proved that PGs@PVA hydrogel promoted healing speed, epithelialization, vascularization, and collagen deposition of diabetic wounds by adaptive immunomodulation. The MMP9-responsive nanozyme hydrogel shows great potential in diabetic wound healing by reconstructing the balance of pro-inflammation and anti-inflammation.
研究领域:
生物材料学
·
医学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCONJUGATE CHEMISTRY
·
2016
·
IF 4.5
Jian Tian,Jie Chen,Cuicui Ge,Xu Liu,Jinlin He,Peihong Ni,Yue Pan
Radiation is one of the most widely used methods for cancer diagnosis and therapy. Herein, we report a new type of radiation sensitizer (Fc-PEG) by a facile one-step reaction of conjugating the hydrophilic PEG chain with hydrophobic ferrocene molecule. The chemical composition and structure of Fc-PEG have been thoroughly characterized by FT-IR, NMR, GPC, and MALDI-TOF mass spectrometry. This Fc-PEG conjugate could self-assemble in aqueous solution into spherical aggregates, and it was found that the exposure to 4 Gy of X-ray radiation have little influence on the shape and size of these aggregates. After the chemical bonding with PEG chains, the uptake level of Fe element could be enhanced via the formation of aggregates. The live/dead, CCK-8, as well as apoptosis assays, indicated that the death of cancer cells can be obviously increased by X-ray radiation after the incubation of these Fc-based nanoconjugates, which might be served as the radiation sensitizer toward cancer cells. We suggest that this radiosensitizing effect comes from the enhancement of reactive oxygen specimen (ROS) level as denoted by both flow cytometric and fluorescence microscopic analysis. The enhanced radiation sensitivity of cancer cells is contributed by the synergic effect of Fe-induced radiation-sensitizing and the increased uptake of nanoconjugates after polymeric grafting.
研究领域:
化学
·
纳米技术
·
癌症研究
·
放射治疗
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 12
·4639
·
IF 4.6
Dafeng Liu,Longguang Jiang,Jincan Chen,Zhuo Chen,Cai Yuan,Donghai Lin,Mingdong Huang
Photodynamic therapy (PDT) is recognized as a powerful method to inactivate cells. However, the photosensitizer (PS), a key component of PDT, has suffered from undesired photobleaching. Photobleaching reduces reactive oxygen species (ROS) yields, leading to the compromise of and even the loss of the photodynamic effect of the PS. Therefore, much effort has been devoted to minimizing photobleaching in order to ensure that there is no loss of photodynamic efficacy. Here, we report that a type of PS aggregate showed neither photobleaching nor photodynamic action. Upon direct contact with bacteria, the PS aggregate was found to fall apart into PS monomers and thus possessed photodynamic inactivation against bacteria. Interestingly, the disassembly of the bound PS aggregate in the presence of bacteria was intensified by illumination, generating more PS monomers and leading to an enhanced antibacterial photodynamic effect. This demonstrated that on a bacterial surface, the PS aggregate photo-inactivated bacteria via PS monomer during irradiation, where the photodynamic efficiency was retained without photobleaching. Further mechanistic studies showed that PS monomers disrupted bacterial membranes and affected the expression of genes related to cell wall synthesis, bacterial membrane integrity, and oxidative stress. The results obtained here are applicable to other types of PSs in PDT.Keywords:photosensitizer (PS);photobleaching;photosensitizer aggregate;photosensitizer monomer
研究领域:
化学
·
生物医学工程
·
微生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Hao Cai,Yifei Niu,Tianyuan Guan,Yin Zhang,Zichuan Ma
The intimate coupling of photocatalysis and biodegradation (ICPB) technology has received much attraction because of the advantages of both photocatalytic reaction and biological treatment. In this study, ZnO–CoFe 2 O 4 @BC (ZCFC) with p-n heterojunction was prepared and used in an ICPB system to degrade metronidazole (MNZ) wastewater. The microstructure, morphology, and optical behavior of heterojunctions in ZCFC were investigated using SEM, XRD , UV–vis, FTIR, and XPS techniques. The results showed that ZCFC inherited the advantages of bamboo biochar's large pore size, and its large pore structure could provide a habitat for bacterial colonization in ICPB, thus shortening the internal mass transfer distance. The degradation of MNZ and chemical oxygen demand (COD) by the ICPB system was 86.8% and 58.5%, respectively, which was superior to single photocatalysis (72.5% for MNZ and 43.8% for COD) and single biodegradation (23.5% for MNZ and 20.1% for COD). In ICPB, photocatalysis and biodegradation showed a synergistic effect in the removal of MNZ, and the order of the major reactive oxygen species (ROS) leading to reduced toxicity of MNZ to the biofilm was •OH > h + > O 2 • - . High-throughput sequencing analysis showed continuous evolution of biofilm structures in ICPB enriched a variety of functional species, among which the electroactive bacteria Alcaligenes and Brevundimonas played an important role in the degradation of MNZ. In this study, we investigated the possible mechanism of photocatalytic and microbial synergistic degradation of MNZ in the ICPB system and proposed a new technology for degrading antibiotic wastewater that combines the advantages of photocatalysis and biodegradation.
研究领域:
环境科学
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2024
·
IF 6.1
Xuwen Da,Yunli Xu,Lei Wang,Xiulian Liu,Yatong Peng,Yao Wu,Wanpeng Zhou,Wentao Wang,Xuesong Wang,Qian-Xiong Zhou
Traditional photodynamic therapy (PDT) is almost ineffective against deeply seated tumors due to the limited penetration depth of external light. The development of chemiexcited PDT is promising to address this issue, but it is still challenging to construct simple, efficient and targeted chemiexcited PDT systems. In this work, CPPO (bio(3,5,6-trichloro-2-n-pentyloxycarbonylphenyl) oxalate) and [Ru(phen)2(PIP-OCH3)](PF6)2 (Ru3, where phen = 1,10-phenanthroline, PIP-OCH3 = 2-(4-methoxy phenyl)-1Himidazo[4,5-f][1,10]phenanthroline) with efficient photo-catalytic NADH oxidation were co-encapsulated with DSPE-PEG 2000 to form Ru3/CPPO@PEG nanoparticles (NP3). Once internalized by cancer cells, Ru3 can be excited by the chemical energy produced by the reaction between CPPO and endogenous H2O2. The subsequent oxidation of intracellular NADH and generation of H2O2 in situ by excited Ru3 will further promote the sustained occurrence of chemical reaction, realizing a self-enhanced chemiexcited PDT. NP3 could effectively deplete intracellular NADH, inhibit ATP synthesis, increase H2O2 content, and produce reactive oxygen species (ROS) within cancer cells, thus exhibited efficient chemiexcited PDT activity against various cancer cells. Notably, minimal cytotoxicity was observed towards normal cells. Good therapeutic effects against subcutaneously implanted and lung metastatic tumors were also observed for NP3. The current findings may provide new sights for development of novel chemiexcited PDT.
研究领域:
光动力疗法
·
癌症研究
·
纳米技术
·
生物化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2023
·
IF 4.9
Qianyu Yang,Yingkun Guo,Yang Zhou,Jiali Song,Yujun Song,Hanmei Li,Huile Gao,Weiyuan Huang
Currently, the low survival rate and poor prognosis of patients with nasopharyngeal carcinoma are ascribed to the lack of early and accurate diagnosis and resistance to radiotherapy. In parallel, the integration of imaging-guided diagnosis and precise treatment has gained much attention in the field of theranostic nanotechnology. However, constructing dual-modal imaging-guided nanotheranostics with desired imaging performance as well as great biocompatibility remains challenging. Therefore, we developed a simple but multifunctional nanotheranostic GdCPP for the early and accurate diagnosis and efficient treatment of nasopharyngeal carcinoma (NPC), which combined fluorescence imaging and magnetic resonance imaging (MRI) onto a single nanoplatform for imaging-guided subsequent photodynamic therapy (PDT). GdCPP had an appropriate particle size (81.93 ± 0.69 nm) and was highly stable, resulting in sufficient tumor accumulation, which along with massive reactive oxygen species (ROS) generation upon irradiation further significantly killed tumor cells. Moreover, GdCPP owned much stronger r1 relaxivity (9.396 mM–1 s–1) compared to clinically used Gd-DTPA (5.034 mM–1 s–1) and exhibited better T1WI MRI performance. Under dual-modal imaging-guided PDT, GdCPP achieved efficient therapeutic outcomes without causing any noticeable tissue damage. The results of in vitro and in vivo studies indicated that GdCPP may be a suitable candidate for dual-modal imaging-guided precision tumor therapy.
研究领域:
诊疗学
·
纳米技术
·
癌症研究
·
成像技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 30
·2214619
·
IF 19
Xiang Sun,Mengshi Wei,Xin Pang,Lin Lin,Qiang Gao,Lichao Su,Ting Liu,Youliang Yao,Jibin Song,Wei Wang,Xiaohui Yan
Sonodynamic bacterial inactivation, a reactive oxygen species (ROS)-empowered approach featuring high penetration depth and low health risk, is explored for antibiotic-free antibacterial treatment. However, the low yield and insufficient diffusion of ROS negatively affect the antibacterial efficacy of sonodynamic treatment, thus hindering its further development. Here an actuator-integrated mechanism is proposed for enhancing the sonodynamic efficacy of loaded sonosensitizers through motion-induced hydrodynamic effects, demonstrated by a porphyrin-decorated gold nanomotor, which can produce ROS for bacterial inactivation while performing multimodal motion via actuation using low-frequency ultrasound. Corroborated by numerical simulation, the experimental results show that the motor's stirring motion significantly increases the yield and diffusion of ROS through fluid flow and frequent interactions between the motor and bacterial targets, resulting in doubled antibacterial efficiency in comparison to a stationary motor. Furthermore, the flow-induced shear forces combined with the frequent interactions constitute a source of mechanical damage and can form a synergy with the antibacterial properties of ROS, enabling an efficient biofilm eradication that is inaccessible by freely suspended porphyrin. In conclusion, this study reports a motion-based strategy to enhance sonodynamic efficacy and provides proof of concept using a sonodynamic gold nanomotor powered by ultrasound.
研究领域:
生物医学工程
·
纳米技术
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2024
·Vol. 19
·期 5
·e0301358
·
IF 2.9
Chuang Wei,Yijun Gao,Peifeng Li
Drug-resistant bacteria arising from antibiotic abuse infections have always been a serious threat to human health. Killing bacteria with toxic reactive oxygen species (ROS) is an ideal antibacterial method for treating drug-resistant bacterial infections. Here, we prepared Pt-Ru bimetallic nanoclusters (Pt-Ru NCs) with higher peroxidase (POD)-like activity than Pt monometallic nanoclusters. Pt-Ru can easily catalyze the decomposition of H 2 O 2 to produce ·OH, thereby catalyzing the transformation of 3,3’,5,5’-tetramethylbiphenylamine (TMB) to blue oxidized TMB (oxTMB). We utilized the POD-like activity of the Pt-Ru NCs for antibacterial therapy. The results showed that at doses of 40 μg/mL and 16 μg/mL, the Pt-Ru NCs exhibited extraordinary antibacterial activity against E . coli and S . aureus , demonstrating the enormous potential of Pt-Ru NCs as antibacterial agents.
研究领域:
纳米技术
·
生物材料
·
抗菌研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 17
·3677-3688
·
IF 6.331
Lei Zhang,Ruihao Yang,Honglian Yu,Zhigang Xu,Yuejun Kang,Hongjuan Cui,Peng Xue
Silk fibroin (SF), derived from Bombyx mori, is a category of fibrous protein with outstanding potential for applications in the biomedical and biotechnological fields. In spite of its many advantageous properties, the exploration of SF as a versatile nanodrug precursor for tumor therapy has still been restricted in recent years. Herein, a multifunctional SF-derived nanoplatform was facilely developed via encapsulating the photosensitizer chlorin e6 (Ce6) into MnO2-capped SF nanoparticles (NPs). SF@MnO2 nanocarriers were synthesized through a surface crystallization technique, using SF as a reductant and sacrificial template. Afterwards, Ce6 was covalently incorporated into the loose structure of the SF@MnO2 nanocarrier on the basis of adsorption to abundant peptide-binding sites. To modulate the tumor microenvironment (TME), SF@MnO2/Ce6 (SMC) NPs were capable of catalyzing the decomposition of H2O2 into O2, which can be converted into cytotoxic reactive oxygen species (ROS) during photodynamic therapy (PDT). Moreover, the MnO2 component was able to oxidize intracellular glutathione (GSH) into non-reducing glutathione disulfide (GSSG), and the consumption of GSH could significantly protect the local ROS from being reduced, which further augmented the therapeutic outcome of PDT. Via another angle, SMC NPs can produce strong hyperthermia under near-infrared (NIR) light activation, which was highly desirable for efficient photothermal therapy (PTT). Both in vitro and in vivo studies demonstrated the intense tumor inhibitory effects as a result of augmented PTT/PDT mediated by SMC NPs. We believe that this study may provide useful insights for employing SF-based nanocomposites for more medical applications in the near future.
研究领域:
生物医学工程
·
纳米医学
·
癌症治疗
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Wenting Li,Zhuoran Yang,Chunyu Yang,Wei Guo
This study addresses the challenge of enhancing ferroptosis efficacy for tumor therapy, particularly the limited therapeutic efficiency of current inducers due to tumor microenvironment constraints. Herein, we developed a hollow ultrasound-triggered ZnFe 2 O 4 -Bi 2 MoO 6 (ZB) S-scheme heterojunction loaded with artesunate (ART) to overcome these limitations. The ZB heterojunction with a particle size of ∼250 nm efficiently separates electron-hole pairs under ultrasound (US), promoting the generation of reactive oxygen species (ROS). The photodynamic effect of ZB further boosts ROS production, while ART, controlled-released by phase change materials under laser/US stimulation, enhances ROS production via Fe 2+ -mediated decomposition. This triple-enhanced strategy accumulates lipid peroxidation (LPO), significantly improving ferroptosis effects with a tumor suppression rate of 94.3 %. Moreover, ZB enables multimodal imaging and stimulates antitumor immunity, demonstrating its potential as a diagnostic and therapeutic agent. Our findings demonstrate the potential of this ZB@ART system in advancing ferroptosis-based tumor therapies, inspiring future designs of efficient ferroptosis inducers.
研究领域:
癌症治疗
·
纳米技术
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）