JOURNAL OF HAZARDOUS MATERIALS
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2024
·
IF 12.2
Jianfei Wang,Wenwei Liu,Haiyang Jiang,Chen Li,Haoran Song,Shugen Liu,Yingjie Li,Senlin Tian,Ping Ning
Air pollution by sulfur dioxide (SO 2 ) remains a pressing concern for both the environment and human health. Desulfurization enhanced by persulfate based advanced oxidation processes (PS-AOPs) has been proven to be a feasible method. However, the inherent contradiction between the rapid diffusion mass transfer of SO 2 in the “gas-liquid-gas” phase and the limited lifespan of reactive oxygen species (ROS) can not be ignored. Excessive investment in PS is required to sustainably generate ROS to achieve continuous desulfurization performance, which may lead to excessive PS consumption. To address this issue, whether PS can achieve the oxidation absorption of SO 2 via a non-reactive oxygen species pathway was investigated. Experimental and computational results demonstrated that peroxymonosulfate (PMS) instead of peroxydisulfate (PDS) had a great SO 2 removal performance, the utilization of PS could be effectively achieved by maintaining a 1:1 molar ratio of PMS and removed SO 2 . The presence of H O O bonds in the PMS introduced a partial positive charge to the oxygen atom, making the PMS polar and more susceptible to be attacked by the nucleophile HSO 3 - . So SO 2 underwent a series of processes including dissolution, dissociation, one-oxygen atom transfer, and ionization before ultimately being converted into SO 4 2- ions, effectively achieving its removal from flue gas . This study may presents a novel approach for achieving high-efficiency flue gas desulfurization.
研究领域:
环境科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
TOXICOLOGY AND INDUSTRIAL HEALTH
·
2023
·
IF 1.9
Xueqi Liu,Wenwu Sun,Jianping Cao,Zhuang Ma
Smoking or occupational exposure leads to low concentrations of acrolein on the surface of the airways. Acrolein is involved in the pathophysiological processes of various respiratory diseases. Reports showed that acrolein induced an increase in mitochondrial reactive oxygen species (mROS). Furthermore, exogenous H₂O₂ was found to increase intracellular Zn2⁺ concentration ([Zn2⁺]ᵢ). However, the specific impact of acrolein on changes in intracellular Zn2⁺ levels has not been fully investigated. Therefore, this study aimed to investigate the effects of acrolein on mROS and [Zn2⁺]ᵢ in A549 cells. We used Mito Tracker Red CM-H2Xros (MitoROS) and Fluozin-3 fluorescent probes to observe changes in mROS and intracellular Zn2⁺. The results revealed that acrolein increased [Zn2⁺]ᵢ in a time- and dose-dependent manner. Additionally, the production of mROS was observed in response to acrolein treatment. Subsequent experiments showed that the intracellular Zn2⁺ chelator TPEN could inhibit the acrolein-induced elevation of [Zn2⁺]ᵢ but did not affect the acrolein-induced mROS production. Conversely, the acrolein-induced elevation of mROS and [Zn2⁺]ᵢ were significantly decreased by the inhibitors of ROS formation (NaHSO₃, NAC). Furthermore, external oxygen free radicals increased both [Zn2⁺]ᵢ levels and mROS production. These results demonstrated that acrolein-induced elevation of [Zn2⁺]ᵢ in A549 cells was mediated by mROS generation, rather than through a pathway where [Zn2⁺]ᵢ elevation leads to mROS production.
研究领域:
呼吸系统疾病
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细胞生物学
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氧化应激
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毒理学
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Research
·
2025
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IF 9.6
Yunsheng Lu,Yibin Huang,Chenchen Mao,Pengfei Shan,Chenkang Wu,Jiongzhou Zhu,Yujie Lin,Zhongyu Li,Mingdong Lu
Silencing NOP2/Sun RNA methyltransferase family member 2 (NSUN2) effectively inhibits gastric cancer (GC) progression but is limited by RNase degradation, rapid renal clearance, and low uptake. Based on the characteristic high levels of reactive oxygen species (ROS) in the tumor microenvironment, this study designed and synthesized a novel ROS-responsive ferrocene nanoparticle loaded with siNSUN2 (PRPFc@siNSUN2). Under ROS conditions, the nanoparticle disintegrates to release siNSUN2. Characterization by proton nuclear magnetic resonance, transmission electron microscopy, dynamic light scattering, and ultraviolet–visible spectrophotometry revealed that PRPFc@siNSUN2 is spherical, with an average diameter of 88.79 ± 1.14 nm, an encapsulation efficiency of 83.10%, and a drug loading capacity of 13.85%. Moreover, these nanoparticles demonstrated excellent stability and, under hydrogen peroxide conditions, exhibited structural disruption leading to the release of siNSUN2, thereby confirming their high ROS responsiveness. In vitro, PRPFc@siNSUN2 markedly enhanced the inhibition of GC cell proliferation, migration, and invasion, and promoted apoptosis, accompanied by increased intracellular ROS and improved siNSUN2 uptake. In vivo studies further confirmed that PRPFc@siNSUN2 markedly enhanced the therapeutic efficacy of siNSUN2 against GC, while exhibiting low cytotoxicity and good biocompatibility. Overall, our findings indicate that PRPFc@siNSUN2, with its favorable morphology, stability, and ROS-triggered release, substantially improves the anti-GC effects of siNSUN2 by inhibiting GC cell proliferation, migration, and invasion, as well as by promoting apoptosis. These results support NSUN2 as a promising therapeutic target and underscore the potential of PRPFc@siNSUN2 nanoparticles in drug delivery, offering a novel strategy to improve clinical outcomes for GC patients.
研究领域:
肿瘤学
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分子生物学
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药剂学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 6
·2913
·
IF 6.208
Man Zhang,Xingfen Wang,Jun Yang,Zhicheng Wang,Bin Chen,Xinyu Zhang,Dongmei Zhang,Zhengwen Sun,Jinhua Wu,Huifeng Ke,Liqiang Wu,Guiyin Zhang,Yan Zhang,Zhiying Ma
Verticillium wilt (VW), a fungal disease caused byVerticillium dahliae, currently devastates cotton fiber yield and quality seriously, yet few resistance germplasm resources have been discovered inGossypium hirsutum. The cotton variety Nongda601 with suitable VW resistance and high yield was developed in our lab, which supplied elite resources for discovering resistant genes. Early nodulin-like protein (ENODL) is mainly related to nodule formation, and its role in regulating defense response has been seldom studied. Here, 41 conserved ENODLs inG. hirsutumwere identified and characterized, which could divide into four subgroups. We found thatGhENODL6was upregulated underV. dahliaestress and hormonal signal and displayed higher transcript levels in resistant cottons than the susceptible. TheGhENODL6was proved to positively regulate VW resistance via overexpression and gene silencing experiments. Overexpression ofGhENODL6significantly enhanced the expressions of salicylic acid (SA) hormone-related transcription factors and pathogenicity-related (PR) protein genes, as well as hydrogen peroxide (H2O2) and SA contents, resulting in improved VW resistance in transgenicArabidopsis. Correspondingly, in theGhENODL6silenced cotton, the expression levels of both phenylalanine ammonia lyase (PAL) and 4-coumarate-CoA ligase (4CL) genes significantly decreased, leading to the reduced SA content mediating by the phenylalanine ammonia lyase pathway. Taken together,GhENODL6played a crucial role in VW resistance by inducing SA signaling pathway and regulating the production of reactive oxygen species (ROS). These findings broaden our understanding of the biological roles ofGhENODLand the molecular mechanisms underlying cotton disease resistance.Keywords:Gossypium hirsutum;GhENODL6;Verticillium wilt resistance;SA;ROS
研究领域:
植物学
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遗传学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Research
·
2024
·
IF 8.5
Pingjun Zhu,Xi Wang,Qingfeng Wu,Jianbo Zhu,Yifan Que,Yan Wang,Yongkai Ding,Yang Yang,Jie Jin,Xin Zhang,Qian Xu,Qinge Yong,Christopher Chang,Guogang Xu,Yingzhen Du
Background: B-cell receptor-associated protein 31 (BCAP31) has protective effects against alveolar epithelial type II cells (AECII) damage by inhibiting mitochondrial injury in acute lung injury (ALI) induced by lipopolysaccharide (LPS), whereas the precise mechanism is still unclear. It is known that PTEN-induced putative kinase 1 (PINK1)/Parkin-mediated mitophagy can remove damaged mitochondria selectively, which may be involved in BCAP31 protection against mitochondrial injury. Methods: In the current study, ALI mice models were established by using surfactant protein C (Sftpc)-BCAP31 transgenic mice (BCAP31TG mice) and AECII-specific BCAP31 knockout mice (BCAP31CKO mice) treated with LPS. Results: BCAP31 expression in lung tissue and AECII were inhibited in ALI mice. Under LPS challenge, lower level of BCAP31 was found to correlate positively with pathological injury of the lung, respiratory dysfunction, mortality rates, inflammation response, and AECII damage. Further study showed that down-regulation of BCAP31 induced decreased phosphorylation of PINK1 via reduced binding to PINK1, thereby restraining PINK1/Parkin-mediated mitophagy. Down-regulation of mitophagy promoted mitochondrial injury, as shown by the increase in mitochondrial permeability transition pore opening rate, together with enhanced mitochondrial reactive oxygen species (mROS), which were accompanied by increased cellular apoptosis and reactive oxygen species (ROS). The increased cellular ROS contributed to the inflammatory response via activation of nuclear factor κB (NF-κB). In contrast, BCAP31 overexpression promoted phosphorylation of PINK1 and PINK1/Parkin-mediated mitophagy, thus blocking the mROS/ROS/NF-κB pathway, favoring a protective condition that ultimately led to the inhibition of AECII apoptosis and inflammatory response in LPS-induced ALI. Conclusion: Ultimately, BCAP31 alleviated ALI by activating PINK1/Parkin-mediated mitophagy and blocking the mROS/ROS/NF-κB pathway in AECII.
研究领域:
免疫学
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肺病学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Peili Li,Shuai Liu,Gaoke Zhang,Xu Yang,Weiwei Cao,Xuedong Gong,Xiaodong Xing
Bacterial biofilm poses a serious threat to human health, leading to increased and prolonged bacterial infections. How to solve the problem of eliminating biofilms effectively and rapidly while being nontoxic to normal cells is still a challenge. Here, we design a pH-sensitive anti-biofilm nanosystem formed by self-assembly between negatively charged carboxyl groups of poly(ethylene glycol_-COOH-polyethylenimine-2,3-dimethylmaleic anhydride (PPD) and positively charged amines on the surface of carbon dots derived from the ashes of calcined l-lysine powder (CDLys) (PPD@CDLys for short). The outmost copolymer could make PPD@CDLys facilely diffuse into the dense biofilm and reverse to be positively charged via hydrolysis, which lead to the acid-triggered disassembly of the nanosystem. After hydrolyzation, PPD would turn into a biocidal cationic polymer, which is prone to attaching on bacteria inside the biofilm and efficiently killing them. In addition, the released CDLys could induce intracellular reactive oxygen species (ROS) across the whole biofilm to degrade the matrix of extracellular polymer substances and kill resident bacteria deep into the biofilm. Finally, the prepared nanosystem effectively inhibits the formation of Staphylococcus aureus biofilm and rapidly destroys the mature biofilm by the synergy antibacterial effects of the cation and ROS. We also evaluate the biocompatibility of the nanocomposites. The results show that PPD@CQDLys has no toxicity to L929 and 3T3 cells and exhibits a zero hemolytic rate even when the concentration is up to 2000 μg/mL. The outstanding biocompatibility coupled with rapid anti-biofilm ability of the nanosystem presents an opportunity for it to be utilized as an effective pH-responsive and targetable anti-biofilm agent for controlling bacterial infections.
研究领域:
生物医学工程
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材料科学
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微生物学
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DOI
PubMed
WATER RESEARCH
·
2022
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IF 13.4
Liping Fang,Baolin Gao,Fangbai Li,Kai Liu,Jialin Chi
The incorporation of secondary metal atoms into iron oxyhydroxides may regulate the surface chemistry of mediating electron transfer (ET) and, therefore, the biogeochemical pollutant processes such as arsenic (As) in the subsurface and soils. The influence of incorporating two typical metals (Cu and Zn) into a specific {001} hematite facet on O 2 activation by surface-bound Fe(II) was addressed. The results showed that Cu-incorporated hematite enhances As(III) oxidation in the presence of Fe(II) under oxic conditions and increases with increasing Cu content. Conversely, Zn incorporation leads to the opposite trend. The As(III) oxidation induced by surface-bound Fe(II) is positively related to the Fe(II) content and is favorable under acidic conditions. Reactive oxygen species (ROS), such as superoxide (·O 2− ) and H 2 O 2 , predominantly contribute to As(III) oxidation as a result of 1-electron transfer from bound Fe(II) to surface O 2 on hematite and radical propagation. Electrochemical analysis demonstrates that Cu incorporation significantly lower the oxidation potential of Fe(II) on hematite, whereas Zn led to a higher reaction potential for Fe(II) oxidation. Subsequently, distinct surface reactivities of hematite for the activation of O 2 to form ROS by surface-bound Fe(II) are evidenced by metal incorporation. Our study provides a new understanding of the changes in the surface chemistry of iron oxyhydroxides because of incorporating metals (Zn and Cu), and therefore impact the biogeochemical processes of pollutants in soils and subsurface environments.
研究领域:
环境科学
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土壤化学
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地球化学
✓ 已验证
DOI
高影响因子
Frontiers in Bioscience-Landmark
·
2025
·Vol. 30
·期 4
·26038
·
IF 3.1
Deqiang An,Xianyao Jiang,Yucheng Yang
Background:Sesamin can suppress many cancers, but its effect on nasopharyngeal carcinoma (NPC) is unclear. Herein, we set out to pinpoint the possible changes in NPC due to Sesamin.Methods:The biological function of NPC cells exposed to Sesamin/N-acetyl-L-cysteine (NAC)/3-Methyladenine (3-MA) was detected, followed by evaluation of reactive oxygen species (ROS) production (dichlorodihydrofluorescein diacetate staining) and mitochondrial membrane potential (MMP) (flow cytometry). Proteins pertinent to apoptosis (cleaved caspase-3, cleaved poly (ADP-ribose) polymerase 1 (PARP1)), cell cycle (Cyclin B1), and autophagy (microtubule-associated protein light chain 3 (LC3)-I, LC3-II, Beclin-1, P62) were quantified by Western blot. After the xenografted tumor model in mice was established, the tumor volume and weight were recorded, and Ki-67 and cleaved caspase-3 levels were determined by immunohistochemical analysis.Results:Sesamin inhibited viability, proliferation, cell cycle progression and migration, induced apoptosis, increased ROS production, and decreased MMP in NPC cells. Sesamin elevated cleaved caspase-3/caspase-3, cleaved PARP1/PARP1, and Beclin-1 expressions as well as LC3-II/LC3-I ratio, while diminishing Cyclin B1 and P62 levels. NAC and 3-MA abrogated Sesamin-induced changes as above in NPC cells. Sesamin inhibited the increase of the xenografted tumor volume and weight, down-regulated Ki-67, and up-regulated cleaved caspase-3 in xenografted tumors.Conclusion:Sesamin exerts anti-tumor activity in NPC, as demonstrated by attenuated tumor proliferation and xenografted tumor volume and weight, as well as induced apoptosis in tumor tissues, consequent upon the promotion of autophagy and reactive oxygen species production.
研究领域:
肿瘤学
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药理学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2022
·Vol. 11
·期 12
·2330
·
IF 7.675
Tian Su,Zhengyi Zhang,Xiao Han,Fei Yang,Zhen Wang,Ying Cheng,Huadong Liu
SIRT1 functions by regulating the modification of proteins or interacting with other proteins to form complexes. It has been widely studied and found to play significant roles in various biological processes and diseases. However, systematic studies on activated-SIRT1 interactions remain limited. Here, we present a comprehensive SIRT1 interactome under resveratrol stimulation through proximity labeling methods. Our results demonstrated that RanGap1 interacted with SIRT1 in HEK 293T cells and MCF-7 cells. SIRT1 regulated the protein level of RanGap1 and had no obvious effect on RanGap1 transcription. Moreover, the overexpression of Rangap1 increased the ROS level in MCF-7 cells, which sensitized cells to resveratrol and reduced the cell viability. These findings provide evidence that RanGap1 interacts with SIRT1 and influences intracellular ROS, critical signals for mitochondrial functions, cell proliferation and transcription. Additionally, we identified that the SIRT1-RanGap1 interaction affects downstream signals induced by ROS. Overall, our study provides an essential resource for future studies on the interactions of resveratrol-activated SIRT1. There are conflicts about the relationship between resveratrol and ROS in previous reports. However, our data identified the impact of the resveratrol-SIRT1-RanGap1 axis on intracellular ROS.Keywords:SIRT1;resveratrol;proximity labeling;RanGap1;ROS
研究领域:
分子生物学
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细胞生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Science Advances
·
2020
·Vol. 6
·期 29
·
IF 13.117
Yufeng Liu,Yuan Cheng,He Zhang,Min Zhou,Yijun Yu,Shichao Lin,Bo Jiang,Xiaozhi Zhao,Leiying Miao,Chuan-Wan Wei,Quanyi Liu,Ying-Wu Lin,Yan Du,Christopher J. Butch,Hui Wei
Here, an integrated cascade nanozyme with a formulation of Pt@PCN222-Mn is developed to eliminate excessive reactive oxygen species (ROS). This nanozyme mimics superoxide dismutase by incorporation of a Mn–[5,10,15,20-tetrakis(4-carboxyphenyl)porphyrinato]–based metal-organic framework compound capable of transforming oxygen radicals to hydrogen peroxide. The second mimicked functionality is that of catalase by incorporation of Pt nanoparticles, which catalyze hydrogen peroxide disproportionation to water and oxygen. Both in vitro and in vivo experimental measurements reveal the synergistic ROS-scavenging capacity of such an integrated cascade nanozyme. Two forms of inflammatory bowel disease (IBD; i.e., ulcerative colitis and Crohn’s disease) can be effectively relieved by treatment with the cascade nanozyme. This study not only provides a new method for constructing enzyme-like cascade systems but also illustrates their efficient therapeutic promise in the treatment of in vivo IBDs.
研究领域:
生物医学工程
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治疗学
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纳米医学
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炎症研究
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产品关联
DOI
PubMed
高影响因子
Theranostics
·
2025
·Vol. 15
·期 12
·5953
·
IF 13.3
Rui Zhang,Danhua Mao,Yiyong Fu,Rong Ju,Guoqing Wei
Background: The pursuit of effective antibacterial strategies aimed at mitigating pathogenic bacterial infections while minimising drug resistance remains of paramount importance. A combinational therapeutic strategy that integrates distinct treatment components can enhance overall efficacy and mitigate undesired effects, thereby exhibiting considerable promise in combating bacterial infections. Methods: In this study, a meticulously engineered self-assembling hybrid nanoprodrug (CPBP NPs) has been devised, functioning as a hybrid prodrug of Ciprofloxacin (Cip) and hydroxybenzyl alcohol (HBA). Results: CPBP molecules can generate nanoassemblies via self-assembly and subsequently undergo decomposition to synchronously release Cip and HBA upon hydrogen peroxide (H 2 O 2 ) exposure. The CPBP NPs exert antibacterial and anti-inflammatory properties through the controlled release of Cip and HBA, while also facilitating the scavenging of reactive oxygen species. These CPBP NPs exhibit broad-spectrum antibacterial activity against both Gram-negative bacteria ( E. coli , 98.4%) and Gram-positive bacteria ( S. aureus , 98.5%). Notably, CPBP NPs not only accumulate in the lungs to facilitate organ-specific infection treatment but also expedite the healing process of infected wounds. Conclusions: Consequently, this H 2 O 2 -activatable hybrid nanoprodrug, possessing excellent biocompatibility, holds substantial promise for advancing clinical applications in managing bacterial infections.
研究领域:
药学
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生物医学工程
·
感染病学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 5
·2701
·
IF 4.9
Shizi Zhang,Yunfeng Xu,Fang Wang,Liyun Yang,Lijuan Luo,Lingyan Jiang
Anthracnose caused byColletotrichum gloeosporioidesis a destructive disease ofStylosanthes(stylo). Combination treatment of phloretin and pterostilbene (PP) has been previously shown to effectively inhibit the conidial germination and mycelial growth ofC. gloeosporioidesin vitro. In this study, the effects of PP treatment on the growth ofC. gloeosporioidesin vivo and the biocontrol mechanisms were investigated. We found that exogenous PP treatment could limit the growth ofC. gloeosporioidesand alleviate the damage of anthracnose in stylo. Comparative transcriptome analysis revealed that 565 genes were up-regulated and 239 genes were down-regulated upon PP treatment during the infection byC. gloeosporioides. The differentially expressed genes were mainly related to oxidative stress and chloroplast organization. Further physiological analysis revealed that application of PP afterC. gloeosporioidesinoculation significantly reduced the accumulation of O2•−level and increased the accumulation of antioxidants (glutathione, ascorbic acid and flavonoids) as well as the enzyme activity of total antioxidant capacity, superoxide dismutase, catalase, glutathione reductase, peroxidase and ascorbate peroxidase. PP also reduced the decline of chlorophyll a + b and increased the content of carotenoid in response toC. gloeosporioidesinfection. These results suggest that PP treatment alleviates anthracnose by improving antioxidant capacity and reducing the damage of chloroplasts, providing insights into the biocontrol mechanisms of PP on the stylo against anthracnose.Keywords:phloretin;pterostilbene;ROS scavenging;chloroplasts;anthracnose;stylo
研究领域:
植物病理学
·
分子生物学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）