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Improving the Extraction Process of Mehlich 3 Method for Calcareous Soil Nutrients

Changqing Li,Shuo Wang,Mengyu Sun,Dongxiao Li,Huasen Xu,Liangyu Zhang,Cheng Xue,Wenqi Ma,Zhimei Sun

Soil nutrient testing is an effective way to uncover soil nutrient status. However, the conventional testing method (CT method) and Mehlich 3 method (M3 method) demand long-time consumption, tedious testing steps, high testing cost, dangerous chemicals contained in extractant, etc. Therefore, it is important to develop a new rapid test method or improve the existing rapid test method of soil available nutrients. In this study, an improved Mehlich3 method (IM3 method) with the new combined extractant were developed and evaluated on the testing feasibility, precision, efficiency, and cost. The results showed that: (1) IM3 method avoided the usage of two hazardous chemicals, i.e., ammonium nitrate and nitric acid, which were difficult in purchase and storage but contained in the combined extractant of M3 method. (2) The correlation coefficients of available P, K, Fe, Mn, Cu and Zn in calcareous soil between M3 and CT, and between IM3 and CT methods all reached highly significant level. The correlation coefficient of available Zn between IM3 and CT method was significantly higher than that between M3 and CT method, and those of the other elements had not obviously changed. (3) The variation coefficients of available P, K, Fe and Cu determined by M3 and IM3 methods were all lower than those determined by CT method. The variation coefficients of available Mn and Zn determined by IM3 were 3.67% and 6.43%, which were slightly higher than those determined by CT method with 2.72% and 5.29%, but were lower than those determined by M3 method. (4) Under the premise of ensuring testing precision, IM3 method took only 6.3 min/piece for determining six elements, reducing testing time by 70.7% and 3.08% compared with CT (21.5 min/piece) and M3 (6.5 min/piece) methods, respectively. The testing cost of IM3 method was reduced by 26% and 61.2% compared with M3 and CT methods, respectively. In conclusion, IM3 method is an ideal rapid measurement method for the simultaneous determination of available soil nutrients in calcareous soil.Keywords:improved testing method for soil nutrients;extractant;testing precision;testing time;testing cost;calcareous soil