ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Xiao Long Xiong,Yun Peng Ma,Hui Liu,Cheng Zhi Huang,Jun Zhou
The determination of pH values is crucial in various fields, such as analytical chemistry, medical diagnostics, and biochemical research. pH test strips, renowned for their convenience and cost-effectiveness, are commonly utilized for pH qualitative estimation. Recently, quantitative methods for determining pH values using pH test strips have been developed. However, these methods can be prone to errors due to environmental factors, such as lighting conditions, which affect the imaging quality of the pH test strips. To address these challenges, we developed an innovative approach that combines machine learning techniques with pH test strips for the quantitative determination of pH values. Our method involves extracting artificial features from the pH test strip images and combining them across multiple dimensions for comprehensive analysis. To ensure optimal feature selection, we developed a feature selection strategy based on SHAP importance. This strategy helps in identifying the most relevant features that contribute to accurate pH prediction. Furthermore, we integrated multiple machine learning algorithms, employing a robust stacking fusion strategy to establish a highly reliable pH value prediction model. Our proposed method automates the determination of pH values through pH test strips, effectively overcoming the limitations associated with environmental lighting interference. Experimental results demonstrate that this method is convenient, effective, and highly reliable for the determination of pH values.
研究领域:
分析化学
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生物信息学
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DOI
PubMed
高影响因子
Bioengineering & Translational Medicine
·
2022
·
IF 10.684
Mengjia Zheng,Yuyue Zhang,Tianli Hu,Chenjie Xu
Microneedle (MN)-based diagnostic devices can efficiently access skin interstitial fluid (ISF) for accurate and minimally invasive detection of health-related biomarkers. This work reports a biomarker (i.e., glucose or alcohol) monitoring MN device that is composed of swellable MNs and electrochemical test strip. This device is constructed by adhering MN patch on the electrochemical strips using the chitosan as the connecting layer. The MNs penetrate the skin for extraction of ISF that flows to the backing layer of MNs and is analyzed by the test strip. In the in vitro skin models, this device accurately detects the glucose from 0 mM to 12 mM and alcohol from 0 mM to 20 mM. In vivo experiment shows this MN device is capable of minimally invasive sampling of ISF and analysis of glucose levels to determine the glycemic status of mice.
研究领域:
生物医学工程
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分析化学
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DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 15
·8529-8536
·
IF 3.049
Geng Yang,Xia Meng,Shimin Fang,Hongdong Duan,Lizhen Wang,Zhenzheng Wang
An efficient fluorescent probe Pyr-Rhy based on pyrazole was developed, which can detect Hg2+ in water. Its fluorescence properties were studied by UV-vis and fluorescence spectroscopy, and the study results indicated that this probe can selectively detect Hg2+via complexation reaction, and then cause a remarkable color change from colorless to pink and a strong fluorescence enhancement can be observed. Furthermore, this probe showed high sensitivity with the detection limit down to 2.07 × 10−8 M, and its stoichiometric ratio toward Hg2+ ions was 1 : 1. The sensing mechanism was investigated by Job's plot 1H NMR titrations, and FT-IR spectra analysis, which demonstrated a chelation-enhanced fluorescence (CHEF) mechanism. More importantly, obvious color changes of sensor Pyr-Rhy can be observed when it was impregnated on filter paper testing strips and immersed in Hg2+ solution (water as solution), indicating its potential application for trace Hg2+ detection in environmental samples.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 3
·034513
·
IF 4.386
Yu Leng,Daijun Yang,Pingwen Ming,Bing Li,Cunman Zhang
Corrosion of stainless steel bipolar plate (BPP) remains a big challenge while it has been considered to be one of the most appropriate candidates for proton exchange membrane fuel cell (PEMFC) in automotive application. This work aims to study comprehensively the effects of temperature, pH, bubbled gas and potential on corrosion behaviours of SS316L. Moreover, SEM and EDX are performed to reveal the evolutions of surface morphology, microstructure and chemical composition of SS316L under different testing conditions. The results indicate that temperature, pH and potential have significant influences on corrosion potential (Ecorr.), corrosion current density (Icorr.) and passive current density (Ip) of SS316L. In addition, interactions between different testing conditions on corrosion resistance are strong. The main corrosion mechanism of SS316L would be shifted from pitting to intergranular or uniform corrosion as pH decreases from 3 ∼ 5 to 0.3 ∼ 1. Accelerated testing solutions with pH in the range of 0.3 ∼ 1 may be suitable to evaluate corrosion resistance of materials for BPP at lower potential. However, simulated testing conditions with pH in the range of 3 ∼ 5 is more appropriate when higher potentials of 1.0 ∼ 1.4 V (vs Ag/AgCl) need to be considered.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Lulin Peng,Maohua Yang,Mei Zhang,Mingyan Jia
Sulfite is widely used in food and drugs as a preservative; however, excessive intake of exogenous sulfite is harmful to human health. Hence, developing effective methods to detect sulfite and its derivatives is vital. Herein, we present a ratiometric fluorescent sensor for bisulfite based on a dual-emission boron-nitrogen double-doped carbon dots (BNCDs)-Cr(VI) system. After adding bisulfite, the fluorescence intensity at 450 nm was significantly enhanced because of the inhibition of the inner filter effect, while the fluorescence emission at 535 nm decreased. A sensor-based method was developed to determine bisulfite with a linearity range of 0 – 10.0 × 10 2 μmol/L and recoveries of 97.33% – 109.00%, achieving the quantitative detection of bisulfite in real sugar samples. Moreover, a paper test strip-based visual measuring system for bisulfite was developed, which realized the rapid and semiquantitative detection of bisulfite.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Biomolecules
·
2025
·Vol. 15
·期 7
·1052
·
IF 4.8
Guo Wei,Chuanliang Wang,Rui Wang,Peng Zhang,Xuhui Geng,Jinhua Li,Abbas Ostovan,Lingxin Chen,Zhihua Song
Aloin, a kind of active phenolic component, is sourced fromAloe vera. Recently, the determination of aloin has received enormous attention, owing to its positive performance (including anti-tumor, antibacterial, detoxification, liver protection, anti-stomach damage, and skin protection activities) and painful side effects (increased carcinogenicity caused by excessive use of aloin) impacting human health. This investigation was inspired by the good fluorescence properties of carbon dots (CDs); CD-based sensors have aroused a great deal of interest due to their excellent sensitivity and selectivity. Thus, it is of great significance to develop novel CD-based sensors for aloin determination. Herein, N,F-CDs were designed and synthesized through a convenient hydrothermal strategy; the synthesized N,F-CDs possessed good fluorescence performance and a small particle size (near 4.3 nm), which demonstrated the successful preparation of N,F-CDs. The resulting N,F-CDs possessed a large Stokes shift and could emit a highly stable green fluorescence. The fluorescence of the N,F-CDs could be effectively quenched by aloin through the inner filter effect. Furthermore, the synthesis procedure was easy to operate. Finally, the N,F-CD-coated test strips were fabricated and combined with a miniaturized fluorimeter for the fluorescence detection of aloin via the inner filter effect for the first time. The N,F-CD-coated test strips were fabricated and used for the fluorescence sensing of aloin, and the results were compared with a typical ultraviolet (UV) method. The N,F-CD-coated test strips exhibited high recovery (96.9~106.1%) and sensitivity (31.8 nM, n = 3), good selectivity, low sample consumption (1 μL), high speed (5 min), good stability, and anti-interference properties. The results indicate that N,F-CD-coated test strips are applicable for the quantitative determination of aloin in bovine serum, orange juice, and urine samples.
研究领域:
分析化学
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生物传感
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纳米材料
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药物分析
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临床诊断
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 3
·661
·
IF 4.2
Bo Hu,Wenshi Zhao,Li Chen,Yang Liu,Zhongfei Ma,Yongsheng Yan,Minjia Meng
Paper-based test strips with on-site visual detection have become a hot spot in the field of target detection. Yet, low specific surface area and uneven deposition limit the further application of test strips. Herein, a novel “turn-on” ratio of molecularly imprinted membranes (Eu@CDs-MIMs) was successfully prepared based on a Eu complex-doped polyvinylidene fluoride membrane for the selective, rapid and on-site visual detection of norfloxacin (NOR). The formation of surface-imprinted polymer-containing carbon dots (CDs) improves the roughness and hydrophilicity of Eu@CDs-MIMs. Fluorescence lifetimes and UV absorption spectra verified that the fluorescence enhancement of CDs is based on the synergistic effect of charge transfer and hydrogen bonding between CDs and NOR. The fluorescent test strip showed a linear fluorescent response within the concentration range of 5–50 nM with a limit of detection of 1.35 nM and a short response time of 1 min. In comparison with filter paper-based test strips, Eu@CDs-MIMs exhibit a brighter and more uniform fluorescent color change from red to blue that is visible to the naked eye. Additionally, the applied ratio fluorescent test strip was combined with a smartphone to translate RGB values into concentrations for the visual and quantitative detection of NOR and verified the detection results using high-performance liquid chromatography. The portable fluorescent test strip provides a reliable approach for the rapid, visual, and on-site detection of NOR and quinolones.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2017
·Vol. 35
·期 4
·447-456
·
IF 1.852
Zhongyu Ding,Hongqing Li,Wanqing Gao,Yiquan Zhang,Chunhua Liu,Yuanyuan Zhu
The synthesis of two linear terpy-based metallo-supramolecular fluorescent coordination polymers through 1∶1 complexation of Zn 2+ and Cd 2+ ions with ditopic terpyridine ligand was reported. The dispersibility of P1 and P2 was significantly improved in organic solvent and water through the introduction of hydrophilic oligo-ethyoxy side chain. Two polymers displayed yellow light emission both in solution and the solid state due to the intra-ligand charge transfer (ILCT) between the d 10 metal ions and the conjugated spacer unit. These coordination polymers were explored as fluorescent chemosensors for detecting picric acid in aqueous media, displaying high sensitivity and good selectivity. In addition, test strips were prepared from these polymers and exhibited the practical potential of detecting the NACs pollutants in the outdoor water for public safety and security.
研究领域:
化学
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材料科学
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环境科学
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DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Ziqiang Yuan,Meizhen Fu,Xin Wang,Meng Wang,Yue Wei,Yanna Sun,Qingfeng Zhang,Yunyi Zhang,Bo Zhang
Nanozymes are nanomaterials with catalytic properties similar to enzymes and offer advantages over natural enzymes such as lower cost, ease of storage, and convenience for large-scale preparation. Thus, they have received increasing attention and are extensively applied in fields such as chemistry, sensing, food, environment, and medicine. Herein, a hemin-derived nanozyme (Hemin-CDs) was prepared using hemin as the precursor and used to substitute the natural horseradish peroxidase (HRP) for colorimetric detection. The prepared Hemin-CDs exhibit excellent water dispersibility, stability and superior peroxidase-like activity. They catalyze the oxidative coupling of colorless 4-aminoantipyrine (4-AAP) with N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) in the presence of H2O2, forming a purple quinone diimine dye with an absorption peak at 554 nm, which enables the quantification of H2O2 by measuring the absorbance. Quantitative detection of glucose was further demonstrated under physiological conditions. The detection of H2O2 and glucose takes only 10 minutes, with linear ranges of 1–150 μM and 2.5–300 μM, and LODs of 0.867 μM and 1.026 μM, respectively. Glucose in human serum was successfully detected with satisfactory recoveries (87.5–104.0%). Furthermore, a test strip was developed, and a smartphone was utilized to recognize the color of the test position, enabling rapid and accurate quantification of glucose concentrations within 5 minutes, which promises to enhance the accessibility and convenience of glucose testing.
研究领域:
生物化学
·
医学诊断
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Panwang Hu,Changkun Xia,Bangxiang Liu,Rulin Feng,Mengzhu Wang,Hengjia Zhu,Xiangheng Niu
Given the huge threat of Hg 2+ to human health, it is always significant to develop Hg 2+ monitoring tools. Utilizing its impacts on nanozyme catalysis is a promising strategy to realize Hg 2+ detection. However, nearly all the assays fabricated based on the principle should be performed in solution and in laboratory and require bulky signal reading instruments, hindering their wider applications. It is highly desired to develop portable, efficient, and practical Hg 2+ sensors. Herein, we proposed the in situ controllable growth of Ag nanoparticles (AgNPs) on paper to fabricate a smartphone optical nanosensor for highly sensitive Hg 2+ detection based on enzyme-mimetic activity stimulation. The ultrasmall AgNPs were directly grown on paper wafers to obtain test strips, which further integrated a 3D-printed holder and an APP built-in smartphone to fabricate the sample-to-answer sensor. The AgNPs-based test strips exhibited a target-stimulated peroxidase-like activity to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine, and the chromogenic information was captured and processed by the smartphone to rapidly report the level of Hg 2+ . With the portable nanosensor, highly sensitive and specific detection of Hg 2+ was achieved, with a detection limit down to 0.44 nM. The sensor gathers the features of easy construction, simple operation, excellent capability, and robust performance together, promising great potential for rapid on-site analysis.
研究领域:
分析化学
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环境科学
·
生物传感
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DOI
高影响因子
JOURNAL OF PHYSICS-CONDENSED MATTER
·
2022
·Vol. 34
·期 30
·304002
·
IF 2.745
Xiaoqing Shi,Marko Kralj,Yang Zhang
The detection of drugs containing hydrochloric salt with conventional methods is time consuming and expensive. In this work, upon exposure to ciprofloxacin hydrochloride at different concentrations, the emission from CsPbBr3 NCs shifts to the blue from 513 nm to 442 nm. CsPbBr(3−x)Clx NCs are formed by the ion exchange and substitution of Br− and Cl− ions from surface to core of NCs. The first-principles calculations suggest that the substitution of Br− by Cl− ions plays a critical role in the tuning of the energy bandgap. The color of paper test strips changes immediately after exposure to different Ciproxan solutions. We propose that this rapid and portable method has a high potential application in other chloride salts for food safety.
研究领域:
分析化学
·
食品安全检测
·
材料科学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Shanglin Li,Yin Gu,Yi Lyu,Yan Jiang,Peng Liu
An integrated graphene oxide purification-lateral flow test strip (iGOP-LFTS) was developed for on-strip purifying and visually detecting polymerase chain reaction (PCR) products with an improved sensitivity as well as a more stringent specificity. PCR products amplified with a pair of biotin- and digoxin-labeled primers were directly pipetted onto GO pads, on which graphene oxide selectively adsorbed residual primers and primer-dimers with the aid of a running buffer containing MgCl2 and Tween 20. By stacking up three GO pads to increase the surface area for adsorption, 83.4% of double-stranded DNA with a length of 30 bp and 98.6% of 20-nt primers could be removed from a 10-μL DNA mixture. Since no primers interfered with detection, the increase of the sample loading volume from 5 to 20 μL could improve the signal-to-noise ratio of the test line 1.6 fold using the iGOP-LFTS while no changes were observed using the conventional LFTS. The limit of detection of the iGOP-LFTS was determined to be 30 copies of bacteriophage λ-DNA with naked eyes and this limit could be further decreased to 3 copies by loading 20 μL of the sample, which corresponded to a 1000-fold improvement compared to that of the LFTS detected by naked eyes. When the ImageJ analysis was employed, a 100-fold decrease of the detection limit can be obtained. In addition, due to the removal of the primer-dimers, the dim test line observed in the negative control of the LFTS was eliminated using the iGOP-LFTS. A mock clinical specimen spiked with defective HIV-1 (human immunodeficiency virus) viruses was successfully analyzed using a two-step reverse transcription-PCR with 30 amplification cycles followed by the iGOP-LFTS detection. These significant improvements were achieved without introducing any additional hands-on operations and instrumentations.
研究领域:
分子生物学
·
分析化学
·
生物医学工程
·
病毒学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）