THERAPEUTIC DRUG MONITORING
·
2025
·
IF 2.4
Dong Zhonghua,Wang Peng,Cui Xueyan,Liu Jinjuan,Shi Haiyan,Yang Yilei,Xu Jingya,Yi Qiaoyan
Background: Perampanel (PER), a novel antiepileptic drug, exhibits high protein binding. Given the drug's susceptibility to various factors, including drug interactions, and the considerable variability in blood concentrations among individuals, solely measuring the total plasma concentration of PER may not provide comprehensive insights. This study aimed to establish an HPLC-FLD method to quantify both total and free PER in clinical samples. Methods: Analysis of total PER involved a straightforward sample preparation process involving plasma protein precipitation. Plasma samples were ultrafiltered to isolate the free portion of PER. Chromatographic separation was achieved on an InertSustain C18 column at a 1-mL/min flow rate using a gradient of acetonitrile and aqueous sodium acetate buffer at pH 4.4. Results: Calibration curves for total and free PER in plasma exhibited excellent linearity over the concentration ranges of 10–3000 and 0.5–100 ng/mL, respectively. The method was applied to analyze blood samples from patients with epilepsy quantitatively; the total concentration of perampanel in the plasma of the patients was in the range of 90.45–563.39 ng/mL, whereas the free concentration was in the range of 1.82–15.29 ng/mL. The plasma protein binding ratio of perampanel was normally between 97.09% and 99.29% and decreased in cases of hypoproteinemia. Conclusions: The method was rigorously validated for selectivity, accuracy, precision, and stability in accordance with established FDA and EMA guidelines. The developed method enables the rapid and accurate quantification of both total and free PER concentrations in the plasma of patients with epilepsy, offering technical support for its subsequent clinical application.
研究领域:
临床药理学
·
分析化学
·
神经病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Qidan Cai,Huaidong Peng,Jiang Meng,Youshao Yan,Yu Zeng,Paul C.H. Li,Yue Sun
In this study, a three-phase laminar flow microfluidic chip (TPL chip) combined with HPLC was developed for monitoring free and total concentrations of paclitaxel (PTX) in blood simultaneously. A diluted whole blood sample (aqueous phase) was introduced into the chip, ethyl acetate (organic phase) was introduced into the chip for extraction, and an interphase was used to prevent the blood sample from coming into direct contact with the organic phase. Because only free drug can quantitatively diffuse into the organic extraction phase and the free drug fraction has a linear relationship with the dilution factor of blood, both the free and total drug concentrations can be obtained by detecting the concentration of paclitaxel in the organic extraction phase. The governing factor such as flow rate for extraction was optimized. Docetaxel was used as an internal standard. The reliability of the quantitative diffusion of molecules in the TPL chip was proved by the methodological investigation of PTX in PBS sample, which showed a good linearity in the concentration range of 0.5 - 100 µg/mL and a detection limit of 7 ng/mL. Good repeatibilities for retention time (RSD of PTX is 1.23%, docetaxel is 1.14%, n = 5) and peak area ratio of PTX to docetaxel (RSD is 4.38%) were obtained. For blood sample analysis, only 100 µL of sample was needed and whole pretreatment was finished in 35 min, and a recovery of 94~117% were obtained. The provided method showed advantages in fast analysis speed, minimum sample handing, and potential ability of automation, and integration.
研究领域:
分析化学
·
药代动力学
·
生物医学工程
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 33
·4179-4186
·
IF 3.1
Xiaoxiao Li,Pan Chang,Xing Liu,Zhongjun Zhao,Wenwen Li,Yi Kang,Yixiang Duan,Wensheng Zhang
Ciprofol (HSK 3486, C14H20O), a novel 2,6-disubstituted phenol derivative similar to propofol, is a new type of intravenous general anaesthetic. We found that the exhaled ciprofol concentration could be measured online by ultraviolet time-of-flight mass spectrometry (UV-TOFMS), which could be used to predict the plasma concentration and anaesthetic effects of ciprofol. In this study, we present the calibration method and validation results of UV-TOFMS for the quantification of ciprofol gas. Using a self-developed gas generator to prepare different concentrations of ciprofol calibration gas, we found a linear correlation between the concentration and intensity of ciprofol from 0 parts per trillion by level (pptv) to 485.85 pptv (R2 = 0.9987). The limit of quantification was 48.59 pptv and the limit of detection was 7.83 pptv. The imprecision was 12.44% at 97.17 pptv and was 8.96% at 485.85 pptv. The carry-over duration was 120 seconds. In addition, we performed a continuous infusion of ciprofol in beagles, measured the exhaled concentration of ciprofol by UV-TOFMS, determined the plasma concentration by high-performance liquid chromatography, and monitored the anaesthetic effects as reflected by the bispectral index value. The results showed that the exhaled and plasma concentrations of ciprofol were linearly correlated. The exhaled ciprofol concentration correlated well with the anaesthetic effect. The study showed that we could use UV-TOFMS to provide a continuous measurement of gaseous ciprofol concentration at 20 second intervals.
研究领域:
药理学
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分析化学
·
麻醉学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2022
·
IF 6.364
Longfei Zhang,Ruite Liu,Zaiyang Wang,Feng Guan,Yanchao Li,Xinqiang Yuan,Long Zhang,Yiguang Jiang
Er 3+ -doped transparent perfluoride composite glass (PFCG) containing SrF 2 crystals was obtained by a one-step method. PFCG was observed to maintain the formation of a single SrF 2 crystal phase even when the Er 3+ doping concentration was as high as 8 mol%. Importantly, Er 3+ was enriched in the crystalline region, which promoted grain growth. This ensures effective emission of upconversion (UC). Interestingly, green and red UC emissions were found to be tunable in the range of 1–6 mol% Er 3+ doping, and the UC emission and lifetime started to produce concentration quenching until 6 mol% Er 3+ doping. To the best of our knowledge, this is the highest quenching concentration of Er 3+ in composite-glass materials. Moreover, the dominant UC process was systematically analyzed at different doping levels. This research is expected to provide ideal candidate materials and appropriate Er 3+ concentration doping level guidance in PFCG for the field of UC lasers.
研究领域:
材料科学
·
光学
·
光子学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Mengdie Lv,Cong Luo,Jian Yang,Yawen Zhou,Changyao Liu,Baocai Xu
Effect of the numbers of oxypropylene on dynamic interfacial tensions (IFT) of five extended surfactants C 12-14 P m E 2 S (m = 0, 3, 5, 8 and 12) were investigated. The effects of electrolyte (NaCl and CaCl 2 ) concentration, salinity, surfactant concentration were studied in detail. The IFTs between C 12-14 P m E 2 S aqueous solutions and n-decane obtained ultralow range at optimum NaCl concentration expect C 12-14 E 2 S. The optimum salinity (S * ) decreased with increase of number of oxypropylene. Also, the IFTs of C 12-14 P 8 E 2 S and C 12-14 P 12 E 2 S approached or obtained ultralow IFT in a wide range of CaCl 2 concentrations. The IFTs between C 12-14 P 12 E 2 S aqueous solution and n-decane decreased as salinity increased. With increasing surfactant concentration, the values of IFT decreased initially and then increased.
研究领域:
化学工程
·
界面化学
✓ 已验证
产品关联
DOI
高影响因子
Green Processing and Synthesis
·
2022
·Vol. 11
·期 1
·445-457
·
IF 3.97
Ziqian Wu,Zhihuan Fu,Yunbo Tian,Murtaza Hasan,Liting Huang,Yuanting Yang,Cuijing Li,Ayesha Zafar,Xugang Shu
The emergence of drug-resistant bacteria and the lack of effective antimicrobial agents have posed a threat to public safety, so a new efficient strategy is needed to deal with the increasing severity of bacteria. Herein, a substitute antibacterial agent with high stability and biocompatibility was synthesized by incorporating lysine hydrochloride and copper sulfate pentahydrate following the liquid-phase synthesis method. The composition and structure of the Cu( ii ) complex (Lys–Cu) were characterized by performing ultraviolet-visible spectrophotometry, Fourier-transform infrared spectroscopy, X-ray diffractometry (XRD), thermogravimetric analysis-differential scanning calorimetry, and single-crystal XRD. The crystal structure of Lys–Cu belongs to a monoclinic system, space group P 2 1 , with cell parameters of a = 5.14350(10), b = 16.8308(2), c = 11.4915(2) Å, Z = 2, and D = 1.548 g·cm −3 . Bacteriostatic tests were carried out on Escherichia coli and Staphylococcus aureus . The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of Lys–Cu against S. aureus were 0.3125 and 0.9250 mg·mL −1 , respectively. The MIC and MBC values of Lys–Cu against bacterial strain E. coli were 0.4685 and 0.9250 mg·mL −1 , respectively.
研究领域:
化学
·
微生物学
·
材料科学
✓ 已验证
DOI
高影响因子
OPTICAL FIBER TECHNOLOGY
·
2025
·
IF 2.7
Xuesong Zhang,Yue Feng,Weixiang Yuan,Haodong Wu,Tao Shen
The detection of ultra-low concentrations of lithium ions (Li + ) is of paramount importance for protecting human health and preserving the ecological environment. This paper proposes a fiber-optic sensor based on lithium/aluminum layered double hydroxides (Li/Al-LDHs) modified optical microfiber coupler (OMC). Firstly, the optimal diameter of the OMC is determined through simulation analysis to optimize the sensor parameters. Secondly, functional group functionalization is employed to facilitate a robust binding of the Li/Al-LDHs to the surface of the optical fiber. Lastly, the efficacy of the sensor in detecting low concentrations of Li + was validated through experimental means. The results demonstrated that the sensor exhibited a maximum detection sensitivity of 334.222 nm/μM and a limit of detection (LOD) of 1.272 nM within the range of 1–10 nM, exhibiting good linearity (R 2 = 0.986). Furthermore, the sensor displayed excellent stability, selectivity and response time, indicating its potential for application in the detection of Li + concentration in aqueous environments.
研究领域:
环境科学
·
光学传感
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2020
·
IF 3.421
Zhao Zhang,Song Zhou,Hongyuan Xi,Majed Shreka
A high nitrate concentration in scrubber water is a major factor that restricts ship applications of NOx wet absorption technology. In this paper, an absorption system (Na2SO3/NaHSO3) that can effectively improve NO2 absorption and reduce the concentrations of nitrate and nitrite was studied. Several factors that affect the NOx absorption efficiency and the concentrations of nitrate and nitrite were investigated in a bubble reactor by using Na2SO3 and Na2SO3/NaHSO3. In addition, the reaction mechanism for the process of NO2 absorption was deduced. The results illustrate that increases in S(IV) concentration and initial NO2 concentration could enhance the NOx absorption efficiency while increases in the NO/NO2 ratio and SO2 concentration decreased the NOx absorption efficiency. Moreover, the maximum NOx absorption efficiency was 97.8% at 0.15 mol/L S(IV) and 1400 ppm of NO2 when using the Na2SO3/NaHSO3 absorption system. The S(IV) concentration was the main factor affecting the NOx absorption efficiency and the concentrations of nitrate and nitrite. Increasing the SO2 concentration slightly affected the NOx absorption efficiency but was beneficial for decreasing the concentrations of nitrate and nitrite. The performance of the Na2SO3/NaHSO3 absorption system was better than that of Na2SO3 in the presence of oxygen. Furthermore, the minimum concentrations of nitrate and nitrite were 13.5 and 0 mg/L, respectively, in the Na2SO3/NaHSO3 absorption system for 1100 ppm of NO2 and 0.3 mol/L S(IV) and were much lower than current regulations for scrubber water. Finally, compared with other experimental results, the Na2SO3/NaHSO3 absorption system exhibits great potential for ship NOx wet absorption, and the addition of NaHSO3 plays a key role in decreasing the concentrations of nitrate and nitrite.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2018
·
IF 2.559
Ning Han,Zhaotao Wang,Guoqi Wang,Jun Yu,Chen Chen,Huiyong Huang,Ruxiang Xu
In this study, we established an in vitro hypoxic system driven by a self-regulated chemical reaction that proved effective for cell culture. The hypoxic device was modified from a 1.5 L polypropylene preservation box normally employed for food storage. Pyrogallic acid, sodium hydroxide, and sodium carbonate were dissolved in water and injected into the box. Sodium dihydrogen phosphate solution was injected into the box after 15 min. We measured the concentrations of oxygen and carbon dioxide in the box to determine viability of the hypoxic system. It maintained low levels of oxygen less than 0.2% and stabilizing levels of carbon dioxide at 5% for at least 96 h. Therefore, this device sustained a stable hypoxic environment that may be applicable for cell culture and in vitro studies of hypoxia.
研究领域:
生物学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2018
·
IF 2.943
Qiong Liu,Ju Li,Xin Zhong,Zan Dai,Zhong Lu,Hao Yang,Rong Chen
In this work, sphere-like Ag/Bi 2 O 3 nanocomposites with the average size of ca. 170 nm were successfully synthesized by simple deposition-precipitation method. The antibacterial activities of as-prepared Ag/Bi 2 O 3 nanocomposites were evaluated by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and colony counting methods. It was found that Ag/Bi 2 O 3 nanocomposites displayed greatly improved antibacterial ability against common pathogenic Gram-positive and Gram-negative bacteria in comparison with single-component Bi 2 O 3 nanospheres. More importantly, Ag/Bi 2 O 3 nanocomposites exhibited remarkably outstanding antibacterial activities against clinical drug-resistant bacteria. The antibacterial activity of Ag/Bi 2 O 3 nanocomposite increased with the increase of Ag content and 15 wt% Ag/Bi 2 O 3 nanocomposites showed the highest antibacterial activity. Furthermore, a plausible antibacterial mechanism of Ag/Bi 2 O 3 nanocomposite was proposed. It was believed that the enhanced generation of H 2 O 2 could lead to the membrane leakage of cytosol and the inactivation of respiratory chain dehydrogenaes, which was possibly responsible for the enhanced antibacterial activities of nanocomposites.
研究领域:
材料科学
·
微生物学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2013
·Vol. 78
·期 1
·M90
·
IF 1.775
Xiaodong Geng,Ronghua Yang,Jianying Huang,Xiao Zhang,Xiangyang Wang
Total of 3 water-soluble quaternary-based chitin/chitosan derivatives, which have an identical molecular weight and anion, were synthesized and characterized. Their antibacterial activities against Salmonella cholerae-suis and Bacillus subtilis were evaluated in vitro . The polysaccharides exhibited the antibacterial efficiency. Their minimum inhibitory concentration (MIC) values vary from 0.02 to 20.48 mg/mL, and their minimum bactericidal concentration (MBC) values vary from 0.08 to 40.96 mg/mL against S. cholerae-suis and B. subtilis , respectively. Futhermore, the extent of Bacillus subtilis cells damage was examined via transmission electron microscopy (TEM) to show how N,N,N -trimethylchitosan (TMC) gradually destroyed and killed B. subtilis cells when they were treated with TMC. One of those quaternary polymers, O -([2-hydroxy-3-trimethylammonium])propyl chitin (OHT-chitin), which can be directly and easily synthesized from chitin in bulk quantities, also was demonstrated its antibacterial activity. These water soluble quaternary-based chitin/chitosan derivatives that have antibacterial effect should be potentially used as antimicrobial agents in many fields. Practical Application: The main practical application behind the investigation and evaluation antibacterial activity of 3 water-soluble quaternary-based chitin/chitosan derivatives could be potentially used as antimicrobial agents in many fields. These polysaccharides represent a renewable source of natural biodegradable polymers and meet with the emergence of more and more food safe problems.
研究领域:
微生物学
·
材料科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Xiaonan Hu,Yang Ye,Yanxi Chen,Min Liu,Wenchao Zhang,Mingshan Zhu
The reaction parameters including catalyst dosage, oxidant amount, initial contaminant concentration and pH etc. play the crucial roles in the heterogeneous persulfate oxidation processes , while the synergistic interactions among these reaction parameters are still obscure. We herein took an efficient heterogeneous persulfate oxidation system “bimetallic Mn x Co 3−x O 4 solid solution (MnCo) activated peroxymonosulfate (PMS)” for carbamazepine (CBZ) removal from water. MnCo/PMS system exhibited outstanding performance that CBZ was completely removed within 10 min. The CBZ degradation performance was ascribed to the radical oxidation of SO 4 ·- and O 2 · - , the nonradical oxidation of 1 O 2 , the redox cycles between Mn and Co species and synergistic interactions among MnCo, PMS and CBZ. By monotonously or synchronously adjusting the MnCo dosage, PMS amount and initial CBZ concentration, the inherent connections of different reaction parameters were established. Strong and different synergistic interactions between MnCo and PMS, and among MnCo, PMS and CBZ, were existed due to the formation of three different reaction modes when reaction parameters met certain conditions. The features of the modes were “two-stage, the following auto-deceleration”, “one-stage, constant velocity” and “two-stage, the following auto-acceleration”. This discovery may provide new insights into the synergistic interactions of reaction parameters in advanced oxidation processes for wastewater treatment .
研究领域:
环境科学
·
催化化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）