Infectious Microbes & Diseases
·
2020
·Vol. 2
·期 1
·26
·
IF 0
Feng Xue-Wen,Shao Jun-Dan,Ji Zhong-Kang,Fang Hong,Ding Cheng,Wang Shu-Ting,Shang-Guan Yan-Wan,Shi Pei,Li Lan-Juan,Xu Kai-Jin
Alternative antimicrobial therapies are urgently needed for a variety of multidrug-resistant bacterial pathogens. Faropenem is an orally available β-lactam in the class of carbapenem antibiotics. Thus far, little information is available on faropenem susceptibility of multidrug-resistant clinical isolates from China. Therefore, in the current study 141 contemporary clinical isolates, including methicillin-resistant Staphylococcus aureus (MRSA: n = 18), coagulase-negative staphylococci (CNS: n = 16), Enterococcus faecalis ( n = 14), Enterococcus faecium ( n = 7), Pseudomonas aeruginosa ( n = 25), Klebsiella pneumoniae ( n = 21), Escherichia coli ( n = 20), and Acinetobacter baumannii ( n = 20), were collected between March 2018 and March 2019 and tested for susceptibility to faropenem and other antimicrobials using the broth microdilution method. All E. faecium , P. aeruginosa , and A. baumannii isolates were fully resistant to faropenem. However, most of the MRSA, CNS, E. faecalis , K. pneumonia , and E. coli isolates appeared to be susceptible to faropenem, with minimal inhibitory concentration 90 values of 4 mg/L, 2 mg/L, 2 mg/L, 4 mg/L, and 2 mg/L, respectively. Therefore, faropenem could be considered as alternative therapy for future treatment of multidrug-resistant MRSA, CNS, E. faecalis , K. pneumonia , and E. coli infections in China.
研究领域:
微生物学
·
传染病学
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药理学
✓ 已验证
DOI
MOLECULES
·
2022
·Vol. 27
·期 21
·7380
·
IF 4.927
Yaoyao Zhu,Liling Wang,Meixu Chen,Yifeng Zhou,Jun Huang
ZhejiangOphiopogonjaponicus(ZOJ) is a specific variety ofOphiopogon japonicuswith characteristic steroidal saponins and homoisoflavonoids, which are also main pharmacodynamic constituents with clinical effects, including curing inflammation and cardiovascular diseases. However, few analysis methods were applied to simultaneously and quantitatively determine two kinds of its constituents, and hazardous organic solvents are mostly used for extraction. In this study, a new validated simultaneous extraction and determination method for four characteristic steroidal saponins and homoisoflavonoids in ZOJ was established by ionic liquid–ultrasonic extraction (IL-UAE) combined with HPLC-DAD-ELSD analysis, which can be used for the quality control of ZOJ. Chromatographic separation was performed with a DAD wavelength at 296 nm, and the ELSD parameters of the drift tube temperature (DTT), atomizer temperature (AT), and nitrogen gas pressure (NGP) were set at 20% heating power, 70 °C, and 25 psi, respectively. The optimal IL-UAE conditions were 1 mol/L [Bmim]CF3SO3aqueous solution, a liquid–material ratio of 40 mL/g, and an ultrasonic time of 60 min. The proposed method is reliable, reproducible, and accurate, which were verified with real sample assays. Consequently, this work will be helpful for the quality control of ZOJ. It can also present a promising reference for the simultaneous extraction and determination of different kinds of constituents in other medicinal plants.Keywords:ionic liquid (IL);ZhejiangOphiopogonjaponicus(ZOJ);steroidal saponins;homoisoflavonoids;HPLC-DAD-ELSD
研究领域:
药用植物学
·
分析化学
·
药用植物
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Yafei Li,Mingfang Zhao,Rui Tang,Keer Fang,Hairui Zhang,Xianjie Kang,Liu Yang,Weihong Ge,Weifeng Du
Ethnopharmacological relevance The quality requirements of Corydalis Rhizoma (CR) in different producing areas are uniform, resulting in uneven efficacy. As a genuine producing area, the effective quality control of CR in Zhejiang Province (ZJ) could provide a theoretical basis for the rational application of medicinal materials. Aim of the study The purpose of this study was to effectively distinguish the CR inside and outside ZJ, and provided a theoretical basis for the quality control and material basis research of ZJ CR. Materials and methods The core components of ZJ CR could be identified by HPLC combined with chemometrics screening, and the quality of CR from different producing areas was evaluated by a genetic algorithm-back propagation (GA-BP) neural network. Chromaticity and near-infrared (NIR) spectroscopy were used to identify CR inside and outside ZJ, and rapid content prediction was realized. The analgesic effect of CR in different regions was compared by a zebrafish analgesic experiment. Analgesic experiments in rats and analysis of the research status of quality components were used to screen the quality control components of ZJ CR. Results The contents of palmatine hydrochloride (YSBMT), dehydrocorydaline (TQZJJ), tetrahydropalmatine (YHSYS), tetrahydroberberine (SQXBJ), corydaline (YHSJS), stylopine (SQHLJ), and isoimperatorin (YOQHS) in ZJ CR were higher than those in CR from outside ZJ, but the content of protopine (YAPJ) and berberine hydrochloride (YSXBJ) was lower than that in CR from outside ZJ. YHSJS and SQHLJ could be used as the core components to identify ZJ CR. The GA-BP neural network showed that the relative importance of ZJ CR was the strongest. Chroma-content correlation analysis and the NIR qualitative model could effectively distinguish CR from inside and outside of ZJ, and the NIR quantitative model could quickly predict the content of CR from inside and outside of ZJ. Zebrafish experiments showed that ZJ, Shaanxi (SX), Henan (HN), and Sichuan (SC) CR had significant analgesic effects, while Hebei (HB) CR had no significant analgesic effect. Overall comparison, the analgesic effect of ZJ CR was better than that of CR outside ZJ. The comprehensive score of the grey correlation degree between YAPJ, YSBMT, YSXBJ, TQZJJ, YHSYS, YHSJS, SQXBJ, and SQHLJ were higher than 0.9, and the research frequency were extremely high. Conclusions The relative importance of the content and origin of most components of ZJ CR was higher than that of CR outside ZJ. The holistic analgesic effect of ZJ CR was better than that of CR outside ZJ, but slightly lower than that of SX CR. YHSJS and SQHLJ could be used as the core components to identify ZJ CR. YAPJ, YSBMT, YSXBJ, TQZJJ, YHSYS, SQXBJ, YHSJS, and SQHLJ could be used as the quality control components of ZJ CR. The multidimensional evaluation method used in this study provided a reference for the quality control and material basis research of ZJ CR.
研究领域:
药用植物学
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中药质量评价
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民族药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 2
·374
·
IF 5.2
Shengyue Zhong,Bin Huang,Teng Wei,Zeyuan Deng,Jing Li,Qiang Wen
Red-flowered oil-tea camellia (ROC) is an important woody oil species growing in the south, and its oil has high nutritional value. There are four main species ofROCin China, namely,Camellia chekiangoleosa(CCH),Camellia polyodonta(CPO),Camellia semiserrata(CSE) andCamellia reticulata(CRE). Reports on the comprehensive comparative analysis ofROCare limited. This study investigated the fruit characteristics and nutritional components of fourROCfruits, and the results showed thatROChad high oil content with levels of 39.13%–58.84%, especially theCCHfruit, which reached 53.6–58.84%. The contents of lipid concomitants ofROCoil were also substantial, including β-amyrin (0.87 mg/g–1.41 mg/g), squalene (0.43 mg/g–0.69 mg/g), β-sitosterin (0.47 mg/g–0.63 mg/g) and α-tocopherol (177.52 μg/g–352.27 μg/g). Moreover, the transverse diameter(TD)/longitudinal diameter (LD) of fruits showed a significant positive correlation with the oil content, andROCfruits with thinner peels seemed to have better oil quality, which is similar to the result of the oil quality evaluation obtained by the gray correlation coefficient evaluation method. FourROCoils were evaluated using the gray correlation coefficient method based on 11 indicators related to the nutritional value ofROC.CCHoil had the highest score of 0.8365, and YS-2 (a clone ofCCH) was further evaluated as the bestCCHoil. Finally, the results of heatmap analysis showed that triglycerides could be used as a characteristic substance to distinguishCCHoil from the other threeROCoils. The PLSDA (Partial least squares regression analysis) model and VIP (Variable important in projection) values further showed that P/S/O, P/O/O, P/L/L, P/L/Ln, S/S/O, S/O/O and P/S/S (these all represent abbreviations for fatty acids) could be used as characteristic differential triglycerides among the fourROCoils. This study provides a convenient way for planters to assess the nutritional quality of seed oil depending on fruit morphology and a potential way to distinguish between variousROCoils.Keywords:red-flowered oil-tea camellia;Camellia chekiangoleosaoil;correlation analysis;gray correlation coefficient;triglycerides
研究领域:
植物学
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营养学
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化学分析
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
·
IF 3.9
Wu Dan,Niu Jingjie,Hu Jianping,Wang Hao,Kuang Haixue
This study aimed to assess the anti-hyperuricemia efficacy of Zhejiang psyllium polysaccharides (ZPP) in rats and to explore its underlying mechanism. Hyperuricemia was induced by intragastric administration of potassium oxonate, hypoxanthine, and adenine. The serum levels of uric acid (UA), creatinine (Cr), and blood urea nitrogen (BUN) were measured, and kidney pathology was examined. Serum metabolomics was employed to monitor metabolic alterations following ZPP intervention. Metagenomic analysis was conducted to investigate the impact of ZPP on the intestinal flora of hyperuricemia rats. The results showed that ZPP could significantly reduce the serum UA level in hyperuricemia rats and exhibited a certain renal protective effect. The metabolomics results indicated that ZPP regulates uric acid levels in rats with hyperuricemia and ameliorates renal pathological changes by modulating biomarkers associated with purine metabolism, amino acid metabolism, and lipid metabolism. Metagenomic research also found that ZPP could increase the relative abundance of uric acid metabolism-related probiotics, such as Limosilactobacillus reuteri and Lactobacillus murinus , thereby improving intestinal flora imbalance in rats with hyperuricemia.
研究领域:
生物化学与分子生物学
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微生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 10
·2518
·
IF 3.098
Xuan Zheng,Hongwei Wang,Peng Zhang,Lin Gao,Ning Yan,Panpan Li,Xinmin Liu,Yongmei Du,Guoming Shen
Chaenomeles speciosa(Sweet) Nakai is a medicinal plant. Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity andα-glucosidase inhibitory activity of the dried fruits ofC. speciosafrom different production regions. In the study, we investigated the chemical components of dried fruits ofC. speciosafrom Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity andα-glucosidase inhibitory activity.C. speciosafrom Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas. Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg). Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.C. speciosafrom Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g). Among four production areas, Yunnan represented the highest antioxidant activity andα-glucosidase inhibitory activity. In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity ofC. speciosa, while total flavonoid and polysaccharide were the main contributors forα-glucosidase inhibitory activity of the plant. These results would be helpful for evaluating the quality ofC. speciosain the different production areas.Keywords:Chaenomeles speciosa;chemical composition;antioxidant activity;α-glucosidase inhibitory activity
研究领域:
药用植物学
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植物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yu-Hui Zhang,Pan-Deng Lei,Yong Ding,Xiao-Ting Zhai,Xiao-Chun Wan,Wei-Xuan Li,Yue Zhang,Hai-Peng Lv,Zhi Lin,Yin Zhu
Huangshan Maofeng (HSMF) is a famous baked green tea from the Anhui Province of China, known for its “clean and fresh” flavor. Zhejiang, another major tea-producing province, focuses on the production of green teas. This study aimed to analyze the characteristic aroma components and specific enantiomeric distribution of significant chiral volatile compounds in HSMF and Zhejiang baked green tea (ZJ-BGT) with respect to their origins, cultivars and grades using stir bar sorptive extraction combined with non-targeted gas chromatography–mass spectrometry (GC–MS) and enantiomeric GC–MS approaches. Unique enantiomeric distributions were identified for 2-methylbutanal, γ-nonanolactone, jasmine lactone, α-pinene, cis -linalool oxide (furanoid), and linalool in HSMF and ZJ-BGT. Furthermore, the concentrations of hexanal, cis -3-hexenyl butyrate, geraniol, and the enantiomeric ratio of R -α-terpineol demonstrated a positive correlation with the HSMF grade. Additionally, S -jasmine lactone and R -γ-nonanolactone present in HSMF, along with S -linalool found in ZJ-BGT, significantly contribute to the flavor quality of their respective teas.
研究领域:
食品科学
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分析化学
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农业化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）