FOOD CONTROL
·
2024
·
IF 5.6
Jiming Wei,Ling Li,Yun Shi,Juncheng Liu,Jiagen Lv,Tuanrui Dong
Given the illegal formaldehyde (FA) contamination in seafood/aqueous products, the instrument-free, chemical-free, low-cost sensor that enables on-site FA detection is of great interest. We have developed a paper-based analytical device (PAD) sensor to detect FA on-site for easy operation by integrating FA-responsive dye decoloration reaction on filter paper and timing decoloration time for quantitative signal interpretation. This innovative dry chemical PAD sensor can be easily prepared. The on-site FA detection is performed by dropping a drop of the sample into the sensor sampling zone and timing the decoloration time, respectively. The difference in decoloration time between a fictitious blank (5.0 μM) and an FA standard is proportional to the FA concentration in the range of 10–700 μM with a detection limit of 5.6 μM. The utility and effectiveness of this PAD sensor has been verified by applying it to seafood, paper cups, and water samples with the supports of official method validation.
研究领域:
食品安全
·
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Chuanhui Huang,Xuezhi Qiao,Weiming Sun,Hui Chen,Xiangyu Chen,Lan Zhang,Tie Wang
Domoic acid (DA) is a naturally occurring neurotoxin known to bioaccumulate in marine products. Despite its hypertoxicity, the enrichment and analysis of trace DA in complex marine organisms remains a challenge. We describe herein the fabrication of a postsynthetic-modified magnetic zeolite imidazolate framework-8 (Fe3O4 SPs@ZIF-8/Zn2+), based on Fe3O4 superparticles, for the adsorption of DA from complex biological matrices. The adsorption of DA is rapid (∼5 min) and occurs through strong electrostatic interactions and chelation with coordinatively unsaturated zinc sites on the surface of Fe3O4 SPs@ZIF-8/Zn2+. Employing our Fe3O4 SPs@ZIF-8/Zn2+ sorbent in a magnetic solid-phase extraction, followed by liquid chromatographic separation and tandem mass spectrometric detection, resulted in a facile, rapid, efficient, and sensitive method for the enrichment and detection of trace DA in marine products. After optimization, this method yielded satisfactory precision (relative standard deviation ≤3.4%; n = 5) with a high degree of linearity from 1.0 to 1000.0 pg mL–1 (r2 = 0.9997) and a detection limit of 0.2 pg mL–1 (S/N = 3). Recoveries of 93.1–102.3% were obtained in spiked aquatic products. In addition, trace levels of DA (49.2 pg mL–1) were found in shellfish samples, confirming the applicability of our Fe3O4 SPs@ZIF-8/Zn2+ adsorbent for the detection of DA in seafood.
研究领域:
食品科学
·
分析化学
·
海洋生物学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Tingting Wang,Yihui Chen,Junfeng Ma,Zhenfeng Jin,Mengsa Chai,Xunwen Xiao,Lihua Zhang,Yukui Zhang
In current study, a new polyethyleneimine (PEI)-modified attapulgite material was prepared serving as a solid support in matrix solid-phase dispersion for the extraction and determination (by atomic absorption spectrometry) of cadmium in seafood products. The major factors affecting PEI grafting were optimized using various PEI amounts and molecular weights . Parameters pivotal to MSPD extraction efficiency, like: pH, volume of eluting solvent, and the sorbent mass-to-sample ratio were investigated. Quantitative recoveries were achieved with 0.21 g of fish sample, 0.13 g of PEI-modified attapulgite dispersing agent , and 50% HNO 3 eluting solution. The limit of detection and the limit of quantification were found to be 2.5 and 8.3 μg kg −1 for cadmium in surimi samples, respectively. The recoveries were in the range of 89.2–100.1%, with RSDs ranged from 3.0% to 7.9% (n = 5). When compared to the method stipulated by the Chinese National Standard GB 5009.15-2014, the newly developed MSPD provides comparable accuracy and even better repeatability for the analysis of the cadmium in real seafood samples.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Xie He,Yong Han,Xueli Luo,Weixia Yang,Chunhua Li,Wenzhi Tang,Tianli Yue,Zhonghong Li
A ratiometric fluorescence (FL) sensor was fabricated by coordinating 2, 6-pyridinedicarboxylic acid (DPA) sensitized Tb 3+ (Tb-DPA) with NH 2 and COOH on the surface of the N -doped carbon dots ( N -CDs) for detecting Hg 2+ in seafood. The sensor exhibited two FL emissions at 436 nm ( N -CDs) as the response signal and at 543 nm (Tb-DPA) as the reference signal when excited at 290 nm. After adding Hg 2+ , the FL emission at 436 nm was significantly quenched and the FL emission at 543 nm was negligibly changed. The electron transfer (ET) between COO − of N -CDs and Hg 2+ led to the FL quenching of N -CDs. The FL ratio (F 436 /F 543 ) exhibited a good linear relationship in the Hg 2+ concentrations of 1 161.51 μM with a low limit of detection (LOD) of ~37 nM. The sensor presented high selectivity, satisfactory accuracy and precision toward Hg 2+ in seafood with recoveries of 86.45–114.47% and RSDs of 0.20–1.92%.
研究领域:
分析化学
·
环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Hongkai Huang,Jiahao Wang,Yuqing Zheng,Weidong Bai,Ya Ma,Xiaojuan Zhao
The discarded longan shell-derived porous carbon material (LPC) served as a scaffold for synthesizing bismuth nanoparticle-loaded longan porous carbon nanocomposite (BiNPs@LPC) via a hydrothermal method . Then BiNPs@LPC was utilized to modify screen-printed carbon electrodes (SPCE) for simultaneous detection of Pb(II) and Cd(II) by square wave anodic stripping voltammetry (SWASV). The material was thoroughly characterized by scanning electron microscopy , X-ray diffraction, Raman spectra , Brunauer-Emmett-Teller analysis, electrochemical impedance spectroscopy and cyclic voltammetry . BiNPs@LPC exhibited abundant porous structures, high surface area , and numerous active sites, which could improve significantly response sensitivity. Under optimal conditions, the peak currents of Pb(II) and Cd(II) exhibited favorable linear relationships with the concentration within a range of 0.1–150 μg L −1 , with detection limits (S/N = 3) of 0.02 μg L −1 and 0.03 μg L −1 , respectively. BiNPs@LPC/SPCE demonstrated remarkable selectivity , stability and repeatability. The proposed method was successfully applied for the detection of Pb(II) and Cd(II) in seafoods achieving satisfying recovery of 97.8%–108.3% and 96.7%–106.4%. These excellent test properties were coupled with convenience for batch preparation of the modified electrodes , highlighting its potential for practical applications in heavy metal detection of real samples.
研究领域:
环境科学
·
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD AND DRUG ANALYSIS
·
2017
·
IF 3.048
Juan Miao,Xin Wang,Yunchang Fan,Jing Li,Lina Zhang,Guitao Hu,Can He,Can Jin
A mercury(II) ion-selective electrode with an ionic liquid (IL), 1-methyl-2-butylthioimidazolium bis(trifluoromethanesulphonyl)imide ([C 1 C 4 Sim]NTf 2 ) as active material was constructed. Parameters affecting the performance of the electrodes such as the dosages of the IL and carbon nanotubes and the aqueous pH values were investigated. Experimental results indicated that the optimal composition of the electrode filling material was 47.6% [C 1 C 4 Sim]NTf 2 , 47.6% tetrabutylphosphonium bis(trifluoromethanesulphonyl)imide (TBPNTf 2 ) and 4.8% carboxylic multi-walled carbon nanotubes (MWCNTs-COOH). Under the selected conditions, the proposed electrodes showed a good linear response in the concentration range of 10 −10 –10 −5 mol L −1 and had a detection limit of 4.1 × 10 −11 mol L −1 . No great interference from common metal ions was found. The proposed electrodes were applied to determine Hg 2+ in seafood samples; the results were comparable to those of the direct mercury analyzer.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Hai Jiang,Zhikang Sun,Qian Guo,Xuan Weng
Vibrio parahaemolyticus is one of the foodborne bacteria that widely present in seafood as well as the leading cause of seafood-associated bacterial gastroenteritis. Traditional identification of such pathogens mainly relies on culturing methods, ELISA or PCR. These methods are usually laborious, time-consuming with poor diagnosis competences, or require costly and bulky equipment though of high sensitivity. In this study, a thread-based microfluidic electrochemical aptasensor was designed, fabricated and tested by using label-free aptamer immunosensing technology for rapid and highly sensitive detection of Vibrio parahaemolyticus in seafood. Both the microfluidic channels and electrodes were simply fabricated on threads. Molybdenum disulfide (MoS 2 ) nanosheets were used to obtain enhanced sensitivity of the electrochemical measurement. When used in detecting Vibrio parahaemolyticus , the proposed aptasensor has a dynamic detection range of 10–10 6 CFU mL −1 with a detection limit of 5.74 CFU mL −1 . Compared with traditional plate counting method, the proposed aptasensor has higher detection sensitivity and less assay time (30 min), while high specificity and accuracy are kept. The proposed microfluidic thread-based electrochemical aptasensor grabs the potential to detect other pathogens by simply functionalizing the threaded electrodes with aptamers for targeted biological substances.
研究领域:
食品安全
·
生物传感技术
·
微生物学
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DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 22
·3537
·
IF 4.7
Siyang Chen,Wenxiu Zhu,Yiqun Zhan,Xiaodong Xia
Although phloretin is widely utilized in the food industry as an additive, its effects on foodborne pathogens remain insufficiently investigated. This study aimed to evaluate the antimicrobial properties of phloretin (PHL) againstVibrio parahaemolyticus(V. parahaemolyticus) and to elucidate the potential mechanisms of action. After PHL treatment, alterations in the cell morphology, cell microstructure, and intracellular contents ofV. parahaemolyticuswere assessed. Scanning electron microscopy revealed substantial damage to cell integrity, subsequent to PHL treatment. A notable reduction in intracellular components, including proteins, ATP, and DNA, was observed in samples treated with PHL. PHL was shown to inhibit the activities ofATPase, β-galactosidase, and respiratory chain dehydrogenase inV. parahaemolyticus. Furthermore, it was demonstrated to elevate the intracellular levels of reactive oxygen species and promote cell death. After being applied to sea bass, shrimp, and oysters, PHL effectively inactivatedV. parahaemolyticusin these seafoods. These findings demonstrate that PHL has potential for application in seafood to controlV. parahaemolyticus.
研究领域:
食品科学
·
微生物学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Jing Gong,Yanlong Chen,Wenwei A.,Xingyuan Zhang,Juanqiong Ma,Zhiyong Xie,Pei Li,Aihua Huang,Shusheng Zhang,Qiongfeng Liao
Persistent organic pollutants (POPs), such as perfluoroalkyl and polyfluoroalkyl substances (PFASs), polychlorinated biphenyls (PCBs), and bisphenols (BPs), have been raising global concerns due to their toxic effects on environment and human health. The monitoring of residues of POPs in seafood is crucial for assessing the accumulation of these contaminants in the study area and mitigating potential risks to human health. However, the diversity and complexity of POPs in seafood present significant challenges for their simultaneous detection. Here, a novel multi-component fluoro-functionalized covalent organic framework (OH-F-COF) was designed as SPE adsorbent for simultaneous extraction POPs. On this basis, the recognition and adsorption mechanisms were investigated by molecular simulation. Due to multiple interactions and large specific surface area , OH-F-COF displayed satisfactory coextraction performance for PFASs, PCBs, and BPs. Under optimized conditions, the OH-F-COF sorbent was employed in a strategy of simultaneous extraction and stepwise elution (SESE), in combination with HPLC−MS/MS and GC−MS method, to effectively determined POPs in seafood collected from coastal areas of China. The method obtained low detection limits for BPs (0.0037 −0.0089 ng/g), PFASs (0.0038 −0.0207 ng/g), and PCBs (0.2308 −0.2499 ng/g), respectively. This approach provided new research ideas for analyzing and controlling multitarget POPs in seafood. Environmental implications Persistent organic pollutants (POPs), such as perfluoroalkyl and polyfluoroalkyl substances (PFASs), polychlorinated biphenyls (PCBs), and bisphenols (BPs), have caused serious hazards to human health and ecosystems. Hence, there is a need to develop a quantitative method that can rapidly detect POPs in environmental and food samples. Herein, a novel multi-component fluorine-functionalized covalent organic skeletons (OH-F-COF) were prepared at room temperature , and served as adsorbent for POPs. The SESE-SPE strategy combined with chromatographic techniques was used to achieve a rapid detection of POPs in sea foods from the coastal provinces of China. This method provides a valuable tool for analyzing POPs in environmental and food samples.
研究领域:
环境科学
·
分析化学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yanbo Wang,Yongzheng Wang,Jian Chen,Shigenobu Koseki,Qing Yang,Hejun Yu,Linglin Fu
Pseudomonas fluorescens and Shewanella baltica are specific spoilage organisms (SSOs) of most seafood products . Both of them can cell-to-cell communicate through quorum sensing (QS), facilitating the spoilage activities such as protease secretion, flagellar movement, and biofilm formation. Thus, it is important to screen the quorum sensing inhibitors (QSI) of P. fluorescens and S. baltica. In this study, we tried to apply a high-throughput virtual screening technology to select QSIs candidates. Three amino acid sequences from the key QS regulator–LuxR/I family (the LuxR and LuxI proteins of P. fluorescens, and the LuxR of S. baltica ) were targeted and analyzed . Their 3D structures were subsequently constructed for the QSIs candidates' screen of both P. fluorescens and S. baltica . The candidates of cytidine-5′-monophosphate (5′-CMP) and 5′-adenylic acid (5′-AMP) with high scores exerted strong antagonistic effects against both P. fluorescens and S. baltica QS. The addition of 5′-CMP and 5′-AMP also inhibit QS-controlled phenotypes (swimming motility, protease activity, EPS, and biofilm formation) in P. fluorescens and S. baltica . Furthermore, the exogenous addition of 5′-CMP and 5′-AMP could retard the spoilage process of salmon slices during low-temperature storage by inhibiting the production of extracellular protease, siderophores, and Total Volatile Basic Nitrogen (TVB-N).
研究领域:
微生物学
·
食品科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Guijiao Zhao,Ying Zhao,Qianqian Wang,Chenhuan Wang,Chun Wang,Zhi Wang,Qiuhua Wu
Endocrine disrupting compounds (EDCs) in food pose severe threats to human wellness, so it is extremely urgent to develop effective method with high sensitivity for detecting EDCs in seafoods. Herein, novel urea-functionalized magnetic porous polymer (M-NB-POP) was synthesized from N,N,N′,N′-tetra( p -aminophenyl)- p -phenylenediamine and 1,3-bis(2-isocyanato-2-propyl)benzene under mild conditions for the first time. M-NB-POP with good recoverability and recyclability shows excellent adsorption for several typical EDCs through hydrogen bonding, π-π and hydrophobic interaction. Using M-NB-POP as magnetic solid-phase extraction adsorbent, an attractive method for effective enrichment of trace EDCs in red shrimp, river prawn and crayfish was successfully developed prior to LC-MS analysis. The proposed method gave a low detection limit of 1.50–7.80 ng g −1 , high spiked recoveries of 86.3–111 %, and small relative standard deviation of 1.6–6.0 %. This work provides a new protocol for assessment of trace EDCs in seafoods and concurrently presents a new strategy for developing functional materials to enrich organic pollutants.
研究领域:
环境科学
·
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Huachun Liu,Yanfang Cheng,Hongwei Zhou,Xinhong Pan,Rongfei Peng,Zhijun Bai,Juntao Li,Lei Tan
Arsenic is a widely studied toxic element that exists in various species with different oxidation states and forms in the environment and biological systems. The different physicochemical properties, environmental behaviors, and toxicities of these arsenic species make speciation analysis essential for environmental monitoring and human health risk assessment. In this study, we demonstrated the determination and monitoring of six arsenic species, including arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenocholine (AsC), and arsenobetaine (AsB) in seafood using high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). We optimized the chromatographic condition and sample preparation for the separation and extraction of arsenic species, and the method was validated with accuracy and reproducibility using certified reference materials and actual samples. Baseline separation of the arsenic species was achieved within 12 min, and oven heat extraction was selected for sample preparation. The method showed excellent linearity for arsenic species from 0.1 to 100 μg L−1, with recoveries ranging from 92% to 123%, intra-day precision of 0.9–5.0%, and inter-day precision of 1.1–19.4%. The method was further used to determine the arsenic species in 109 mollusks, 195 crustaceans, and 195 marine fish samples from Guangdong, China. Distinctive patterns of arsenic species distribution were observed among different seafood categories. AsB was consistently the predominant species across all samples, with a detection frequency higher than 97.9% and contributed to more than 90% of total arsenic, other arsenic species showed marked variations in their occurrence and concentrations.
研究领域:
分析化学
·
环境科学
·
食品安全
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）