Marine Drugs
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2019
·Vol. 17
·期 8
·438
·
IF 3.772
Xiaoyang Liu,Zixu Wang,Fawen Yin,Yuxin Liu,Ningbo Qin,Yoshimasa Nakamura,Fereidoon Shahidi,Chenxu Yu,Dayong Zhou,Beiwei Zhu
In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides.Keywords:zinc-chelating peptide;sea cucumber;chelation mechanism
研究领域:
生物化学
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营养学
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DOI
PubMed
高影响因子
ACS Nano
·
2024
·
IF 15.8
Cewen Hu,Bin Liu,Xinyue Huang,Zhilong Wang,Kaiqi Qin,Luyi Sun,Zengjie Fan
Muscle atrophy resulting from peripheral nerve injury (PNI) poses a threat to a patient’s mobility and sensitivity. However, an effective method to inhibit muscle atrophy following PNI remains elusive. Drawing inspiration from the sea cucumber, we have integrated microneedles (MNs) and microchannel technology into nerve guidance conduits (NGCs) to develop bionic microneedle NGCs (MNGCs) that emulate the structure and piezoelectric function of sea cucumbers. Morphologically, MNGCs feature an outer surface with outward-pointing needle tips capable of applying electrical stimulation to denervated muscles. Simultaneously, the interior contains microchannels designed to guide the migration of Schwann cells (SCs). Physiologically, the incorporation of conductive reduced graphene oxide and piezoelectric zinc oxide nanoparticles into the polycaprolactone scaffold enhances conductivity and piezoelectric properties, facilitating SCs’ migration, myelin regeneration, axon growth, and the restoration of neuromuscular function. These combined effects ultimately lead to the inhibition of muscle atrophy and the restoration of nerve function. Consequently, the concept of the synergistic effect of inhibiting muscle atrophy and promoting nerve regeneration has the capacity to transform the traditional approach to PNI repair and find broad applications in PNI repair.
研究领域:
生物医学工程
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神经科学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2019
·Vol. 99
·期 14
·6400-6407
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IF 2.422
Xiaoyang Liu,Zixu Wang,Jing Zhang,Liang Song,Deyang Li,Zixuan Wu,Beiwei Zhu,Yoshimasa Nakamura,Fereidoon Shahidi,Chenxu Yu,Dayong Zhou
BACKGROUND Zinc is known to play an essential role in the biological activities in the human body. In this study, a zinc-chelating peptide (ZCP) produced by Alcalase-assisted hydrolysis of the body wall of sea cucumber was isolated and identified. The ZCP was purified stepwise by ultrafiltration, anion-exchange chromatography, and gel filtration chromatography, in conjunction with ultraviolet–visual (UV–visual) spectrophotometry, which was used to analyze each purified fraction. RESULTS Analysis of the purified ZCP revealed that its zinc-chelating ability was 33.31%. Analysis of isothermal titration calorimetry suggested that the binding of ZCP and zinc (N ≈ 2) was endothermic, with weak binding affinity. Fourier transform infrared spectroscopy spectra (FTIR) indicated that carboxylic and amide groups in ZCP were the primary binding sites of Zn. Sequencing the result by ultra-performance liquid chromatography-quadrupole/time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) showed that a representative ZCP had the sequence WLTPTYPE with a molecular weight of 1005.5 Da. CONCLUSION These results provide a promising foundation for the production of zinc supplements from sea-cucumber-derived ZCPs. © 2019 Society of Chemical Industry
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 3
·590
·
IF 3.098
Yujing Qin,Qingxia Yuan,Yuexing Zhang,Jialu Li,Xinjiao Zhu,Lingling Zhao,Jing Wen,Jikai Liu,Longyan Zhao,Jinhua Zhao
Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumberPhyllophorus proteus(PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted ofN-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides fromPhyllophorus proteusmay be novel agents having potential value for antioxidation.Keywords:sea cucumber;polysaccharide;enzyme-assisted extraction;characterization;antioxidant activity
研究领域:
海洋生物学
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生物化学
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药学
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DOI
PubMed
高影响因子
AQUACULTURE
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2016
·
IF 1.893
Ningning Liu,Shanshan Zhang,Weiwei Zhang,Chenghua Li
Recently, the use of probiotics as an alternative strategy to control bacterial diseases in aquaculture has garnered much attention due to the merits of environmental friendliness. In this study, a bacterium identified as Vibrio sp. V33 was isolated from healthy sepia and was determined to have strong antagonistic activity towards the pathogenic isolate Vibrio splendidus Vs using the disk diffusion method. The antagonistic substance was secreted into the supernatant and was thermo-stable. The antagonistic activity of Vibrio sp. V33 may be attributed to its higher ability to compete for iron than that of V . splendidus Vs, as deduced from siderophore quantification using the CAS method. Number of colony counts from seawater and Apostichopus japonicus infection experiments showed that Vibrio sp. V33 significantly decreased the quantity of V . splendidus Vs in natural seawater and promoted the survival rate of A . japonicus infected by V . splendidus , with a relative percentage survival of 43% under the current conditions. Our findings suggest that V33 could be potentially used as potential bacterial antagonist for controlling V . splendidus infections in sea cucumber aquaculture. Statement of relevance It might protect A . japonicus from V . splendidus infection.
研究领域:
水产养殖学
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微生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Xin Wen,Xin Zhang,Wanting Wang,Fuqiang Zhao,Mingyong Xie,Guomin Pei,Jun Cao,Hesham El-Seedi,Hui Ye,Jun Yang,Yuanping Zheng,Chuan Li
Diabetic kidney disease (DKD) is a severe metabolic disorder that typically results from diabetes mellitus and culminates in chronic kidney disease and renal failure. It has been reported that Holothuria leucospilota polysaccharide (HLP) has an improvement effect on type 2 diabetes mellitus (T2DM) rats. This study aimed to explore the impact of HLP on DKD further and reveal its mechanism. The spontaneous Goto-Kakizaki (GK) rats were used as a model of the DKD group, and Wistar rats were used as a normal control group, treated with intragastric administration for 4 weeks. The results showed that after HLP intervention, the renal index was reduced. Scr, BUN, and UA levels increased in the DKD group, and the HLP-H group was significantly decreased by 15.44 μmol/L, 1.11 mmol/L, and 0.08 mmol/L ( p < 0.05). HLP also alleviated renal pathological damage and kidney fibrosis, decreased MIF, CD74, and IL-6 mRNA expression levels, enhanced PI3K, AKT, and PCX levels. These results indicated that HLP effectively relieved DKD by regulating inflammation and metabolism.
研究领域:
医学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
AQUACULTURE INTERNATIONAL
·
2025
·Vol. 33
·期 3
·1-18
·
IF 2.4
Kong Lingnan,Abulizi Abudula,Cong Jingjing,Meng Weihan,Chang Yaqing,Sun Zhihui
The cytochrome P4503A ( CYP3A ) subfamily has been well characterized in vertebrates, where it plays a pivotal role in xenobiotic detoxification and the regulation of steroid hormone levels. However, their molecular features and functions in invertebrates remain poorly understood. The sea cucumber ( Apostichopus japonicus ) is not only a commercially significant mariculture species but also an excellent model for studying organ development and evolutionary biology. In this study, we identified seven CYP3A genes in A. japonicus using gonadal transcriptomic data. These genes exhibited ubiquitous expression across adult tissues, with significantly higher levels in ovarian tissues compared to testes tissues. Protein sequence analysis revealed that the catalytic residues Ala 306 -Thr 310 -Cys 437 , which are critical for aromatase activity, are highly conserved. This suggests that CYP3A proteins may mediate aromatization-like reactions similar to CYP19A in vertebrates. Additionally, dietary administration of the aromatase inhibitor letrozole led to histological changes in the testes and a reduction in the diameter of large oocytes in the ovaries. These findings provide valuable insights into the molecular mechanisms underlying gonadal development in sea cucumbers and lay the groundwork for future research on the artificial induction of sex reversal in echinoderms.
研究领域:
分子生物学
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海洋生物学
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进化生物学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2025
·
IF 6
Jiaze Yan,Chao Jiang,Jiqi Zheng,Ming Guo
Background Microdialysis is an in vivo technique for dynamically analyzing biochemical components in tissues and fluids. Traditional microdialysis systems are primarily designed for low molecular weight polar compounds, resulting in lower recoveries for hydrophobic compounds. This study aimed to develop a greener, cost-effective, and enhanced microdialysis method for recovering eugenol, isoeugenol, and methyl isoeugenol, utilizing hydrophobic deep eutectic solvents as the perfusate, with the method's feasibility verified through in vivo experiments. Results Among the 19 types of deep eutectic solvents evaluated, thymol-levulinic acid was identified as the most suitable for eugenol compounds based on liquid-liquid microextraction and microdialysis experiments, showing an in vitro microdialysis recovery approximately 100 times higher than that of water. The optimization of microdialysis parameters was conducted, including molar ratio of HBA:HBD, temperature, flow rate, and semi-permeable membrane length. In the in vivo microdialysis experiments, the temporal concentration of eugenol, which was used as an anesthetic in Glyptocidaris crenularis, was detected by GC-MS/MS and calibrated using ex vivo recovery. When the concentration of eugenol in artificial seawater was maintained at 100 μg mL −1 , the eugenol levels in the coelomic fluid of Glyptocidaris crenularis rose from 0 to 6.89 ± 0.38 μg mL −1 within 60 min, then stabilized in next 80 min. Significance The findings demonstrate that deep eutectic solvents enhanced microdialysis provides high recoveries and enables real-time monitoring of drug concentration changes in biological systems, making this method a valuable tool for pharmacokinetic studies and environmental monitoring.
研究领域:
分析化学
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药理学
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海洋生物学
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Ronghua Yin,Lutan Zhou,Na Gao,Lisha Lin,Huifang Sun,Dingyuan Chen,Ying Cai,Zhili Zuo,Kaifeng Hu,Shengxiong Huang,Jikai Liu,Jinhua Zhao
Glycosaminoglycans (GAGs) are conserved polysaccharides composed of linear repeating disaccharides and play crucial roles in multiple biological processes in animal kingdom. However, saccharide-branched GAGs are rarely found, except the fucose-branched one from sea cucumbers. There was conjecture about the presence of disaccharide-branched GAG since 30 years ago, though not yet confirmed. Here, we report a GAG containing galactose-fucose branches from Thelenota ananas. This unique branch was confirmed as d-Gal4S(6S)-α1,2-l-Fuc3S by structural elucidation of oligosaccharides prepared from T. ananas GAG. Bioassays indicated that oligomers with a larger degree of polymerization exhibited a potent anticoagulation by targeting the intrinsic tenase. Heptasaccharide was proven as the minimum fragment retaining the anticoagulant potential and showed 92.6% inhibition of venous thrombosis in vivo at sc. of 8 mg/kg with no obvious bleeding risks. These results not only solve a long-standing question about the presence of disaccharide-branched GAG in Holothuroidea, but open up new opportunities to develop safer anticoagulants.
研究领域:
生物化学
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海洋生物学
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药理学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Wenjiao Yang,Dingyuan Chen,Zhicheng He,Lutan Zhou,Ying Cai,Hui Mao,Na Gao,Zhili Zuo,Ronghua Yin,Jinhua Zhao
A glycosaminoglycan was isolated from the sea cucumber Holothuria coluber (HcFG). A series of oligosaccharide fragments (dp range 3–11) were prepared from its β -eliminative depolymerized product (dHcFG). Extensive NMR characterization of the oligosaccharides indicated the d -GlcA- β 1,3- d -GalNAc 4S6S repeating disaccharide backbone was substituted by monosaccharide branches comprising of Fuc 2S4S , Fuc 3S4S and Fuc 4S , linked to O-3 of d -GlcA. For the prevailing Fuc 3S4S at nonreducing end of dHcFG, the β- eliminative depolymerization process of HcFG was compared with those of the FGs from Actinopyga miliaris (AmFG, branched with Fuc 3S4S ) and Stichopus variegatus (SvFG, branched with Fuc 2S4S ). The result suggested that d -GlcA substituted with Fuc 3S4S was more susceptible to depolymerization than that with Fuc 2S4S . It might be due to the larger steric hindrance effects from Fuc 2S4S on the esterification of GlcA . Biological assays confirmed that the minimum chain length (dp8), regardless of the Fuc branch types, was required for the potent anti-iXase and anticoagulant activities in FG fragments.
研究领域:
生物化学
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海洋生物学
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药理学
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DOI
高影响因子
AQUACULTURE RESEARCH
·
2016
·Vol. 48
·期 5
·2278-2290
·
IF 1.606
Jing Qian,Chunhua Ren,Ting Chen,Jianjun Xia,Zonghe Yu,Yan Gao,Chaoqun Hu
The broad-spectrum protease inhibitor alpha-2-macroglobulin (α 2 M) is widely found in vertebrates and invertebrates. However, no research regarding α 2 M has been described in sea cucumbers to date. In this study, the first sea cucumber α 2 M named Stm α 2 M was identified from Stichopus monotuberculatus , a tropical species with high edible and economic values in Southern China. The Stm α 2 M cDNA is 5276 bp in length, containing a 5′-untranslated region (UTR) of 279 bp, a 3′-UTR of 344 bp and an open reading frame of 4653 bp that encodes a protein of 1550 amino acids. The Stmα 2 M possesses all the three characteristics of known α 2 M proteins, including the bait region domain, thioester domain and receptor-binding domain. Higher levels of mRNA expression were noticed in intestine, respiratory tree, coelomocytes and tentacle of S. monotuberculatus . Transcriptional expression of Stm α 2 M showed the strongest response to lipopolysaccharides (LPS, 8.19-fold up-regulation) after 6 h post-challenge in coelomocytes. In addition, the α 2 M activity was detected in S. monotuberculatus and protein activity of α 2 M increased remarkably at 6 h after LPS stimulation. As a whole, this study indicated that Stmα 2 M is an important immune-related molecule, and the study on functions of Stmα 2 M may provide some new and valuable ideas for preventing and controlling diseases of cultured sea cucumbers.
研究领域:
免疫学
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分子生物学
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海洋生物学
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DOI
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Zelong Zhao,Xuda Wang,Jingwei Jiang,Ying Dong,Yongjia Pan,Xiaoyan Guan,Bai Wang,Shan Gao,Zhong Chen,Zunchun Zhou
The mariculture environment is a sink of microplastics (MPs) due to its enclosed nature and mass use of plastics. Nanoplastics (NPs) are MPs with a diameter <1 μm that have a more toxic effect on aquatic organisms than other MPs. However, little is known about the underlying mechanisms of NP toxicity on mariculture species. Here, we performed a multi-omics investigation to explore gut microbiota dysbiosis and associated health problems induced by NPs in juvenile sea cucumber Apostichopus japonicus , a commercially and ecologically important marine invertebrate. We observed significant differences in gut microbiota composition after 21 days of NP exposure. Ingestion of NPs significantly increased core gut microbes, especially Rhodobacteraceae and Flavobacteriaceae families. Additionally, gut gene expression profiles were altered by NPs, especially those related to neurological diseases and movement disorders. Correlation and network analyses indicated close relationships between transcriptome changes and gut microbiota variation. Furthermore, NPs induced oxidative stress in sea cucumber intestines, which may be associated with intraspecies variation in Rhodobacteraceae in the gut microbiota. The results suggested that NPs were harmful to the health of sea cucumbers, and they highlighted the importance of the gut microbiota in the responses to NP toxicity in marine invertebrates.
研究领域:
环境科学
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海洋生物学
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）