Yang Xin,Zhu Liying,Jiang Ling,Xu Qing,Xu Xian,Huang He
Background Trehalose has many advantages due to its inertness and the ability to stabilize biomolecules. Trehalose synthase can catalyze intramolecular rearrangement of the inexpensive maltose into trehalose in a single step, which represents a simple, fast, and low-cost method for the future industrial production of trehalose. Results In this work, an intelligent visualization method for producing trehalose via trehalose synthase was for the first time established and optimized by corresponding enzymatic hydrolysis with kudzu root starch as the initial raw material. For the first step, kudzu root starch was liquefied by α-amylase at a certain dextrose equivalent value of 19–21, and β-amylase and pullulanase to saccharify for a certain time, a four-factor nine-level experimental design with nine experiments was performed according to the uniform design table U
* 9
(9 4 ) to optimize the yield of maltose. Then, optimization of trehalose conversion ratio from kudzu root starch hydrolysate was carried out with a four-factor 12-level experimental design to forecast the optimal process conditions. By comparing verification tests and predicted results, the optimized operating conditions were pH 7.1, 22 °C, 32.5 % (14,000 U/ml) loading amounts of TreS enzyme and after 24 h, along with a 70.6 % trehalose conversion rate. Conclusions The intelligent visualization method has succeeded in exploiting the conversion process of trehalose from kudzu root starch hydrolysate, which contributes significant benefits to the further commercial production of trehalose.
In this study, ACP-1, a water-soluble polysaccharide was isolated from the roots of Alocasia cucullata (AC) using freeze-thaw treatment (FTT). Structural characterization revealed ACP-1 (2.10×10 5 Da) to be a homogeneous heteropolysaccharide primarily consisting of glucose, galactose, and arabinose, and a trace of fucose, rhamnose, and glucuronic acid. Methylation analysis and nuclear magnetic resonance spectroscopy revealed that the backbone of ACP-1 consisted of →[3)-β-D-Gal p -(1] 4 →3,6)-β-D-Glc p -(1→3,6)-β-D-Glc p -(1→, with a branch at C-3. In vitro experiments demonstrated that ACP-1 significantly enhanced the proliferation and phagocytosis of RAW264.7 cells, upregulated the expression of co-stimulatory molecules (CD80 and CD86), and activated RAW264.7 cells via the nuclear factor kappa-B signal transduction pathway, resulting in nitric monoxide release and the secretion of the cytokines tumor necrosis factor-α, interleukin (IL)-1β, and IL-6. Overall, ACP-1 shows potential as a novel immunostimulant.
危险品化学品经营许可证（带存储）