Gang Li,Ming Chu,Yingying Tong,Yuexin Liang,Shenghui Wang,Chengjun Ma,Zhenhua Wang,Wenna Zhou
Hippophae rhamnoidesL. is a deciduous shrub that contains many unique bioactive substances. This sea buckthorn possesses anticancer, antioxidant, anti-inflammatory, and cardiovascular protective properties. Herein, the effects of phenylpropyl compounds extracted fromH. rhamnoidesL. on doxorubicin (Dox)-induced cardiotoxicity were evaluated in zebrafish. Cardiac injury in zebrafish was induced using 35 μM Dox for 96 h, and 30 μM phenylpropanoid compounds were used as the protective treatment. The cardioprotective effects and mechanisms of the four phenylpropanoids were investigated using microscopy, behavioral analysis, acridine orange staining, western blotting, flow cytometry, and real-time quantitative polymerase chain reaction. The extracted phenylpropanoids could significantly relieve Dox-induced cardiac injury in zebrafish and inhibit cardiomyocyte apoptosis. The mechanisms of action were mainly related to the stability of mitochondrial biogenesis and function maintained by phenylpropanoids in zebrafish. To our knowledge, this is the first report on the protective effect of sea buckthorn against myocardial injury in zebrafish. Our findings provide support for the further research and development of sea buckthorn and its components.Keywords:sea buckthorn;zebrafish;doxorubicin;cardiotoxicity;mitochondria
Jie Sheng,Mahmoud Youssef,Hongshan Liang,Jing Li,Bin Li
Improving sea buckthorn flavonoids (SF) stability and bioacccessibility is of more practical significance for evaluating the total bioacccessibility of such foods. Therefore, we prepared nanoparticles using zein and gum Arabic (GA) by anti-solvent precipitation to encapsulate SF. Nanoparticles were characterized and assessed for their effect on the stability, release, bioaccessibility, absorption, and antioxidant properties of SF in the in vitro digestion and cell line. The uniform, regular nanoparticles achieved 77.19 % encapsulation efficiency. The SF retention rates during long-term storage (60.46 %) and in vitro digestion (53.76 %) demonstrated that nanoparticles exhibited good stability and bioaccessibility compared to free SF. Furthermore, cellular absorption, the free radical scavenging rate for ABTS (85.26 %) and DPPH (80.48 %), as well as inhibitory effects on intracellular ROS further demonstrated that bilayer nanoparticles could successfully protect and exert biological characteristics of SF. This study evaluated the overall bioaccessibility of sea buckthorn flavonoids using a nanoparticle delivery system, which holds even greater significance for guiding the development of this category of natural foods.
危险品化学品经营许可证（带存储）