Bioresources and Bioprocessing
·
2015
·Vol. 2
·期 1
·1-8
·
IF 0
Yang Xin,Zhu Liying,Jiang Ling,Xu Qing,Xu Xian,Huang He
Background Trehalose has many advantages due to its inertness and the ability to stabilize biomolecules. Trehalose synthase can catalyze intramolecular rearrangement of the inexpensive maltose into trehalose in a single step, which represents a simple, fast, and low-cost method for the future industrial production of trehalose. Results In this work, an intelligent visualization method for producing trehalose via trehalose synthase was for the first time established and optimized by corresponding enzymatic hydrolysis with kudzu root starch as the initial raw material. For the first step, kudzu root starch was liquefied by α-amylase at a certain dextrose equivalent value of 19–21, and β-amylase and pullulanase to saccharify for a certain time, a four-factor nine-level experimental design with nine experiments was performed according to the uniform design table U
* 9
(9 4 ) to optimize the yield of maltose. Then, optimization of trehalose conversion ratio from kudzu root starch hydrolysate was carried out with a four-factor 12-level experimental design to forecast the optimal process conditions. By comparing verification tests and predicted results, the optimized operating conditions were pH 7.1, 22 °C, 32.5 % (14,000 U/ml) loading amounts of TreS enzyme and after 24 h, along with a 70.6 % trehalose conversion rate. Conclusions The intelligent visualization method has succeeded in exploiting the conversion process of trehalose from kudzu root starch hydrolysate, which contributes significant benefits to the further commercial production of trehalose.
研究领域:
生物化学
·
食品科学
·
工业微生物学
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产品关联
DOI
Food Packaging and Shelf Life
·
2024
·
IF 8.5
Honglian Guo,Yue Xu,Haiyi Chen,Xinyu Si,Menghan Zhou,Enlong Zhu
To extend the shelf-life of cherry tomatoes infected with Botrytis cinerea , this study analyzed the effect of Pichia membranifaciens (PM) on a composite film of gelatin (Gel) and trehalose (Tre), and explored the effect of different concentrations of trehalose on the yeast in the bioactive film and its control effect on cherry tomatoes. Scanning electron microscopy showed that Tre/Gel-based film can be applied as effective carriers to protect the cellular integrity of PM. Fourier-transform infrared spectroscopy, thermogravimetric analysis, and tests of mechanical properties showed that there was interaction between PM and the Tre/Gel base film, which maintained the thermal stability of the base film. The addition of PM reduced the water vapor permeability, hygroscopicity, and moisture content of the Tre/Gel base film, effectively extending the active film's storage time. This study also explored the growth curve of PM and found that 15% trehalose was effective in maintaining PM activity and could result in a 30-day survival rate of 62% for PM in Tre/Gel films, with 72.41% inhibition of B. cinerea . Finally, the PM/Tre/Gel film significantly reduced the incidence of decay in cherry tomatoes and improved the storage quality and fungal resistance of the fruit. Thus, PM/Tre/Gel films may be applied as a new antifungal packaging material for the preservation of fresh fruit.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
高影响因子
VETERINARY RESEARCH COMMUNICATIONS
·
2025
·Vol. 49
·期 5
·1-10
·
IF 2
Liu Shuang,Qiu Bo,Li Yue,Wang Hai-Peng,Zhong Zhao-Jin,Wang Lin
Glyphosate (Gly) is a globally prevalent herbicide, which poses significant health risks to human and animals via the food chain. The kidney is the primary excretory organ and a site of residual accumulation during Gly exposure. Trehalose (Tre) has been shown to prevent kidney injury induced by various toxicants due to its anti-apoptotic properties. However, the potential protective effect of Tre on Gly-induced kidney damage in poultry remains unclear. Thus, 60 one-day-old healthy Hy-line Brown roosters were randomly divided into three groups ( n = 20), i.e., control group (basal diet without Gly), Gly group (200 mg Gly/kg basal diet), and Gly + Tre group {(200 mg Gly plus 5 g Tre)/kg basal diet}. The experiment lasted for 120 days, and serum and kidney samples were finally collected for respective analysis. Data first showed that Tre treatment can significantly alleviate Gly-induced renal injury in roosters, as evidenced by serum levels of creatinine and blood urea nitrogen as well as renal histopathological observations. Tre also significantly inhibited the elevated abundance of renal injury marker proteins KIM-1 and NGAL induced by Gly. Meanwhile, Tre obviously alleviated Gly-induced ultrastructural abnormalities in the nuclei of renal tissues. Additionally, Tre markedly inhibited Gly-induced apoptotic assays in rooster kidneys, as demonstrated by the results from TUNEL staining and the protein abundance of apoptotic markers cleaved caspase-3 and cleaved PARP. In summary, these findings suggest that Tre exerts its protective effects against Gly-induced kidney damage in roosters via inhibiting apoptosis.
研究领域:
毒理学
·
兽医学
·
分子生物学
·
环境卫生
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Yan Song,Fengming Gu,Yijiangcheng Li,Weihong Zhou,Fu-an Wu,Jun Wang,Sheng Sheng
The general cutworm, Spodoptera litura (Lepidoptera: Noctuidae) is a worldwide destructive omnivorous pest and the endoparasitoid wasp Meteorus pulchricornis (Hymenoptera: Braconidae) is the dominant endoparasitoid of S. litura larvae. Trehalase is a key enzyme in insect trehalose metabolism and plays an important role in the growth and development of insects. However, the specific function of trehalase in parasitoid and host associations has been less reported. In this study, we obtained two trehalase genes ( SlTre1 and SlTre2 ) from our previously constructed S. litura transcriptome database; they were highly expressed in 3rd instar larvae. SlTre1 was mainly expressed in the midgut, and SlTre2 was expressed highest in the head. SlTre1 and SlTre2 were highly expressed 5 days after parasitization by M. pulchricornis . Treatment with the trehalase inhibitor validamycin A significantly inhibited the expression levels of SlTre1 and SlTre2 , and the trehalase activity. Besides, the content of trehalose was increased but the content of glucose was decreased 24 h after validamycin A treatment in parasitized S. litura larvae. In addition, the immune-related genes in phenoloxidase (PO) pathway and fatty acid synthesis-related genes in lipid metabolism were upregulated in parasitized host larvae after validamycin A treatment. Importantly, the emergence rate, proportion of normal adults, and body size of parasitoid offspring was decreased in parasitized S. litura larvae after validamycin A treatment, indicating that validamycin A disrupts the trehalose metabolism of parasitized host and thus reduces the fitness of parasitoid offspring. The present study provides a novel perspective for coordinating the application of biocontrol and antibiotics in agroecosystem.
研究领域:
昆虫学
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生物化学
·
分子生物学
·
寄生虫学
·
农业生态学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2014
·Vol. 172
·期 6
·3042-3053
·
IF 1.687
Shi Xiaojian,Miao Yelian,Chen Jie Yu,Chen Jun,Li Wenli,He Xun,Wang Jining
Freeze-tolerant baker’s yeasts are required for the processing of frozen doughs. The present study was carried out to investigate the cell survival rate after frozen storage and the change of fermentability in dough due to frozen storage, and to discuss quantitatively the relationship of freeze tolerance with intracellular trehalose, amino acids, and glycerol, using six types of baker’s yeasts as the test materials. The experimental results showed that the fermentability of yeast cells in frozen dough was strongly correlated with the cell survival rate. The baker’s yeast with a higher level of cell survival rate had a larger increase in the total intracellular compound content after frozen storage, and the cell survival rate increased linearly with increasing total intracellular compound content in frozen yeast cells. Trehalose was a primary compound affecting freeze tolerance, followed by glutamic acid, arginine, proline, asparagic acid, and glycerol. The basic information provided by the present study is useful for exploring the freeze-tolerance mechanisms of baker’s yeast cells, breeding better freeze-tolerant baker’s yeast strains, and developing more effective cryoprotectants.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 18
·15240-15246
·
IF 3.289
Cong-Heng Chen,Ting Yao,Qiang Zhang,Yan-Ming He,Li-Hui Xu,Min Zheng,Guang-Rong Zhou,Yuan Zhang,Hong-Jie Yang,Ping Zhou
Abnormal denaturation and aggregation of human amylin or islet amyloid polypeptide (IAPP) into amyloid fibrils has been implicated in the pathogenesis of type 2 diabetic mellitus. Trehalose, a super-hydrophilic molecule, has been shown to prevent denaturation of biomolecules when they are under environmental stress. In this work, we sought to investigate the effects of trehalose on the fibrillation and aggregation of human IAPP (hIAPP) by using circular dichroism spectrum, thioflavin-T fluorescence spectrum, dynamic light scattering, transmission electronic microscopy, atomic force microscopy and quartz crystal microbalance. We demonstrated that (1) the conformation of hIAPP changed from α-helix to β-sheet, followed by fibrillation and aggregation, (2) a low dose of trehalose (under 100 mM) inhibited or delayed the conformation transition of hIAPP and (3) a high dose (more than 500 mM) induced the conformation transition, and promoted the fibrillation and aggregation of hIAPP. These findings are in agreement with the hypothesis of the water replacement and volume exclusion effect on the proteins. The lower concentration of trehalose could replace the water molecules surrounding the hIAPP, and interact with proteins through hydrogen bonding, leading to a reduction in the protein interaction itself, and therefore inhibiting or delaying the protein fibrillation and aggregation. In contrast, the higher concentration of trehalose might interact with itself to form macromolecular clusters, acting as a crowding agent, leading to the hIAPP molecules being excluded by the trehalose clusters and interacting between each other, and therefore promoting the hIAPP fibrillation and aggregation.
研究领域:
生物化学
·
分子生物学
·
医学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xiao Huang,Taisong Pan,Jian Shao,Qianwan Qin,Ming Li,Weichang Li,Wei Sun,Yuan Lin
The unsatisfactory performance of Zn metal anodes significantly impedes the commercial application of aqueous zinc-ion batteries (AZIBs). Herein, we introduce a trace amount of a multifunctional trehalose additive to enhance the stability and reversibility of Zn metal anodes. The trehalose additive exhibits a stronger Zn2+ ion affinity due to abundant lone-pair electrons, disrupting hydrogen bonds in H2O, regulating solvation structures, and tuning the Zn–electrolyte interface. Consequently, the Zn metal anode demonstrates a remarkable Coulombic efficiency of 99.80% and a cycle stability exceeding 4500 h at 1 mA cm–2. Even under stringent conditions of 10 mA cm–2, the Zn metal anode maintains a cumulative capacity of 2500 mA h cm–2 without a short circuit. Furthermore, Zn//Zn symmetric batteries exhibit excellent low-temperature cycle performance (over 400 h at −10 °C). As a proof of concept, assembled Zn//NH4V4O10 and Zn//MnO2 pouch cells demonstrate an improved electrochemical performance. This work presents an electrolyte additive strategy for achieving stable zinc anode operation in AZIBs.
研究领域:
电化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL MICRODEVICES
·
2019
·Vol. 21
·期 1
·1-13
·
IF 2.327
Shen Yiren,Du Kun,Zou Lili,Zhou Xiaoming,Lv Rong,Gao Dayong,Qiu Bensheng,Ding Weiping
Freeze-drying is a promising approach for the long-term storage of erythrocytes at room temperature. Studies have shown that trehalose loaded into erythrocytes plays an important role in protecting erythrocytes against freeze-drying damage. Due to the impermeability of the erythrocyte membrane to trehalose, many methods have been developed to load trehalose into erythrocytes. However, these methods usually require multistep manual manipulation and long processing time; the adopted protocols are also diverse and not standardized. Thus, we develop an osmotically-based trehalose-loading microdevice (TLM) to rapidly, continuously, and automatically produce erythrocytes with loaded trehalose. In the TLM, trehalose is loaded through the erythrocyte membrane pores induced by hypotonic shock; then, the trehalose-loaded erythrocytes are rinsed to remove hemoglobin molecules and cell fragments, and the extracellular solution is restored to the isotonic state by integrating a rinsing-recovering design. First, the mixing function and the rinsing-recovering function were confirmed using a fluorescent solution. Then, the performance of the TLM was evaluated under various operating conditions with respect to the loading efficiency of trehalose, the hemolysis rate of erythrocytes ( ϕ ), the recovery rate of hemoglobin in erythrocytes ( φ ), and the separation efficiency of the TLM. Finally, the preliminary study of the freeze-drying of erythrocytes with loaded trehalose was accomplished using the TLM. The results showed that under the designated operating conditions, the loading efficiency for human erythrocytes reached ~21 mM in ~2 min with a ϕ value of ~17% and a φ value of ~74%. This study provides insights into the design of the on-chip loading of trehalose into erythrocytes and promotes the automation of life science studies on biochips.
研究领域:
生物技术
·
细胞生物学
·
微流控
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Tongchao Su,Youtong Zhang,Wenkai Du,Jie Zeng,Haiyan Gao
The trehalose (TL) was modified with sodium alginate, starch, and the effects of modified trehalose particle on the gluten protein and steamed bread properties were investigated. The results showed that for dough frozen after 30 d, the addition of 0.8 % modified TL significantly increased water-holding capacity by 12.12 %, improved the freezing rate, and the change of binding water could be better controlled. Besides, the α-helix content and random coil content of gluten protein was increased and decreased after adding modified TL, respectively, improving the ordered structure of wheat gluten protein and maintaining its aggregation characteristics and structural stability to some extent. Meanwhile, the brightness, water-holding capacity and specific volume of steamed bread was increased after adding 0.8 % modified TL, which improved and maintained the normal texture and internal structure of steamed bread. The results can provide a basis for the application of trehalose in flour products.
研究领域:
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Insects
·
2021
·Vol. 12
·期 4
·370
·
IF 2.769
Zuo-min Shao,Jian-hao Ding,De-lei Jiang,Zhi-xiang Liu,Yi-jiangcheng Li,Jiao Wang,Jun Wang,Sheng Sheng,Fu-an Wu
Simple SummarySericulture has always been threatened byGlyphodes pyloalisWalker (G. pyloalis). Trehalase is an essential enzyme in chitin metabolism and energy supply. In this study, two trehalase genes inG. pyloalis(GpTre1andGpTre2) were identified and functionally analyzed. Knockdown of the two genes led to the significant downregulation of chitin metabolism pathway-related genes, the difficulty in molting of larvae, and the deformation of adult wings. Moreover, the trehalase inhibitor, Validamycin A, treatment increasedGpTre1andGpTre2expression and affected the expressions of chitin metabolism pathway-related genes. The inhibitor also caused a significantly increased cumulative mortality of larvae. The results suggested thatGpTre1andGpTre2played a vital role onG. pyloalisdevelopment, which could be useful for providing information for insect pest control in the future.AbstractGlyphodes pyloalisWalker (G. pyloalis) is a serious pest on mulberry. Due to the increasing pesticide resistance, the development of new and effective environmental methods to controlG. pyloalisis needed. Trehalase is an essential enzyme in trehalose hydrolysis and energy supply, and it has been considered a promising target for insect pest control. However, the specific function oftrehalaseinG. pyloalishas not been reported. In this study, two trehalase genes (GpTre1andGpTre2) were identified from our previous transcriptome database. The functions of thetrehalasein chitin metabolism were studied by injecting larvae with dsRNAs and trehalase inhibitor, Validamycin A. The open reading frames (ORFs) of GpTre1 and GpTre2 were 1,704 bp and 1,869 bp, which encoded 567 and 622 amino acid residues, respectively. Both ofGpTre1andGpTre2were mainly expressed in the head and midgut. The highest expression levels of them were in 5th instar during different development stages. Moreover, knockdown both ofGpTre1andGpTre2by the dsRNAs led to significantly decreased expression of chitin metabolism pathway-related genes, includingGpCHSA,GpCDA1,GpCDA2,GpCHT3a,GpCHT7,GpCHSB,GpCHT-h,GpCHT3b,GpPAGM,andGpUAP, and abnormal phenotypes. Furthermore, the trehalase inhibitor, Validamycin A, treatment increased the expressions ofGpTre1andGpTre2, increased content of trehalose, and decreased the levels of glycogen and glucose. Additionally, the inhibitor caused a significantly increased cumulative mortality ofG. pyloalislarvae on the 2nd (16%) to 6th (41.3%) day, and decreased the rate of cumulative pupation (72.3%) compared with the control group (95.6%). After the activities of trehalase were suppressed, the expressions of 6 integument chitin metabolism-related genes decreased significantly at 24 h and increased at 48 h. The expressions ofGpCHSBandGpCHT-h, involved in chitin metabolism pathway of peritrophic membrane in the midgut, increased at 24 h and 48 h, and there were no changes toGpCHT3bandGpPAGM. These results reveal thatGpTre1andGpTre2play an essential role in the growth ofG. pyloalisby affecting chitin metabolism, and this provides useful information for insect pest control in the future.Keywords:Glyphodes pyloalisWalker;trehalase;chitin metabolism;Validamycin A;RNA interference;pest control
研究领域:
昆虫学
·
分子生物学
·
害虫防治
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2017
·Vol. 119
·期 9
·1600364
·
IF 2.145
Zhen Wen,Jing Lin,Junqing Su,ZongKun Zheng,Qing Chen,Lidi Chen
The aim of the present work is to evaluate influences of doping trehalose into solid lipid nanoparticles (SLNs) on drug loading. SLNs were prepared by the emulsion-evaporation and low temperature-solidification method, using glyceryl monostearate as a lipid, phosphatidylcholine as a surfactant, trehalose as a modifier and resveratrol as a model drug. As the SLNs were doped by trehalose at a low amount [ m (trehalose): m (lipids) = 1:5], entrapment efficiency and loading capacity were enhanced to (81.37 ± 1.50) and (8.71 ± 0.13)%, respectively. Based on morphology, phase transformation and crystalline structure of SLNs, trehalose was doped into lipid matrix in a non-crystalline state, disrupting the lipid crystallinity and inducing lattice defects.The lipid matrix was crystallized in polymorphic forms composed of α-form and β′-form and β-form, resulting in prevention of drug expulsion from the lipid matrix. The trehalose was interacted with the lipid, facilitating the formation of trehalose/lipid eutectic dopant. The dopant contained lattice defects, allowing for the incorporation of more resveratrol in a amorphous state, contributing to increase of entrapment efficiency and loading capacity. These results confirm stabilizing effects of doping trehalose on improvement of drug loading are attributed to the lipid crystal type and structure of lipid matrix containing lattice defects. Practical applications: Trehalose-doping is an innovative approach to the improvement of loading properties of SLNs. Compared with ordinary SLNs, SLNs doped with trehalose at low amounts exhibit higher drug loading, presenting the potential utility of these nanoparticles as a drug delivery system for hydrophobic drugs. Trehalose modification on improvement of drug loading of SLNs are attributed to the lipid crystal type and structure of lipid matrix containing lattice defects induced by doping trehalose.
研究领域:
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jing Lv,Guangyu Liu,Huimin Gao,Yutao He,Xiaorong Tang,Ziqi Wang,Kaikai Sun,Kulbaram Bayazitova,Zhongliang Jiang
Trehalose, as a food and feed additive, can regulate physiological and pathological processes by activating autophagy, yet the molecular mechanism of trehalose dominated a rise in autophagy has not been elucidated. This study investigated the mechanisms of trehalose-induced autophagy in porcine granulosa cells (PGCs). Trehalose was internalized into PGCs by endocytosis and caused a temporary change of the lysosome pH, ultimately inducing autophagy. Furthermore, miRNA-seq analysis of PGCs indicated that trehalose upregulated the expression of miR-221-5p_R-4, which can target nuclear receptor binding factor 2 (NRBF2) to induce autophagy of PGCs. In summary, this study reveals that miR-221-5p_R-4 targeting NRBF2 was involved in the autophagy of PGCs challenged by trehalose, which was taken up by PGCs through endocytosis.
研究领域:
分子生物学
·
细胞生物学
·
生殖生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）