Haozhen Li,Shuyao Wang,Xiaohua Zhang,Kangkang Song,Long Yang
Tea quality in greenhouse was certain gap with open air. Metabolites and foliar microorganisms were investigated under seaweed fertiliser (CF) and gibberellin (CH) treatments using sensory evaluation, HPLC, untargeted metabolomics, 16S rDNA, and Internal Transcribed Spacer. CF tea was mellow, less astringent, and of better quality compared to CH. Catechin, −(−)Epicatechin, and Epigallocatechin were notably lower in CF. Differentially accumulated metabolites (DAMs) were notably enriched in Flavonoid and Phenylpropanoid biosynthesis, both involved in Catechin synthesis. DAMs in these pathways appeared down-regulated in CF. The CF improved quality by down-regulating metabolites in Phenylpropanoid biosynthesis in conjunction with microbial community metabolism enriched in amino acid and secondary metabolite biosynthesis. Metabolite- microbial correlation analysis indicated that the highest correlation with phenylpropane pathway metabolites was in bacteria Variovorax and Pseudomonas , and in fungi Filobasidium . The study provides theoretical basis for regulating flavour quality of greenhouse tea.
Seaweed fertilizer refers to fertilizer created by extracting the active ingredients from raw seaweed that is then applied to plants as a nutrient source to promote growth and increase yield. A quick method of analysing seaweed fertilizer quality has practical value. In this work, we established a simple electrochemical method for analysing commercial seaweed fertilizer by comparing the contributions of each electro-active ingredient. The electrochemical profiles of 3-hydroxyphenylacetic acid, gibberellin, lycine, abscisic acid, indole-3-acetic acid and zeatin were first recorded in a database. Then, the electrochemical profiles of four commercial seaweed fertilizers were recorded and examined to analyse the contribution of each ingredient.