EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2021
·Vol. 123
·期 8
·2100006
·
IF 2.679
Qiang Zhang,Dongyi Liang,Juan Guo,Rui-Xue Guo,Yongguang Bi
This study aims to produce an inclusion complex between β-cyclodextrin (β-CD) and sea buckthorn fruit oil (SBFO) by using the saturated aqueous solution method and then to compare the resultant solution with the crude mixtures. In this study, the SBFO is extracted by Soxhlet extraction, which is optimized through the single-factor test. The main constituent of the SBFO is identified by gas chromatography-mass spectrometry (GC-MS), which confirms eight fatty acids. Fourier transform infrared spectroscopy, thermogravimetric analysis-differential scanning calorimetry, optical microscopy, scanning electron microscopy, and the result-characterized synthesized compounds confirm the formation of stable inclusion compounds by the SBFO with β-CD. The yield values of the inclusion complex and the SBFO loading content are 64.87 ± 2.26% and 9.2 ± 1.58%, respectively. Comparing the crude fruit oil, β-CD–SBFO, and saponified fruit oil reveals differences in their 2,2-diphenyl-1-pycrylhydrazyl and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate) antioxidant activities. These findings suggest that the SBFO inclusion complex after β-cyclodextrin is stabilized and is a good antioxidant. This solution improves the inadequacy of the water solution and the instability of the SBFO. This study also implies that β-CD is a cost-effective and straightforward carrier for extending the applications of SBFO as a food or medicinal additive. Practical applications : In this experiment, the SBFO is converted from oil to powder to increase its efficacy as an additive to health and food products.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Food Science and Technology
·
2023
·
IF 2.602
LI Ying,LEE Xiangjun,GUO Jianfeng,FENG Jingjing,XU Chenshuai,BAI Yujie,GUO Shiwei,WANG Fang
Palmitoleic acid (C16:1n7, POA), a 16-carbon ω-7 monounsaturated functional fatty acid, has been demonstrated a variety of beneficial properties to human health and a wide range of applications in nutrition, medical and chemical industries. Lipozyme® RM IM (Rhizomucor Miehei lipase), a Sn-1,3 specific lipase, was used to catalyze the hydrolysis of seabuckthorn fruit oil and produce monoglycates rich in palmitoleic acid. The fruit oil of seabuckthorn was used as the research object, the dosage of enzyme taking 10% wt.% oil, the influence of water/oil ratio, pH value and reaction temperature to hydrolysis rate were investigated, and then the response surface methodology was applied for optimizing the process conditions. Further, the relationship between the hydrolysis rate and the content of monoacylglycerol was studied under the optimal hydrolysis conditions. The result indicated that the optimal combined condition is as follows: the reaction temperature is 55°C, the pH is 7, the water-oil quality ratio is 1:1. The hydrolysis rate achieved 79.59% within 12 h in the condition above. Under the optimal conditions, the hydrolysis rate was positively correlated with the content of monoester, and the correlation coefficient was 0.999 within the first 10 h of the reaction. The hydrolysis rate was 66.76% at 10 h, and the content of monoglyceride reached the highest of 61.11%. Hereafter, the hydrolysis of monoglyceride began if the reaction continued. The content of palmitoleic acid increased from 27.11% to 40.84%, and that of linoleic acid increased from 4.51% to 33.34%, so the total amount of the two functional fatty acids reached 74.18%. Enzymatic hydrolysis can be an effective way to improve the function of seabuckthorn fruit oil.
研究领域:
食品科学
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生物化学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yaping Zheng,Kangmeng Sun,Xinyuan Sun,Yue Li,Peigen Xiao,Chunnian He
Sea buckthorn is rich in active ingredients, widespread, and has both medicinal and nutritional value. The present comparative study of wild and cultivated species remains insufficient, which is not conducive to their quality control. Therefore, this study aimed to compare the differences of 21 sea buckthorn samples in total phenolic content (TPC), total flavonoid content (TFC), phenolic components content, secondary metabolites, and antioxidant capacity and the fatty acid, to investigate the quality differences of different varieties. The TPC, TFC and antioxidant activity of wild varieties were higher than those of the cultivated. Multivariate statistical analysis revealed large differences in phenolic content, with higher levels of gallic acid and isorhamnetin-3- O -neohesperidin in the wild, whereas the cultivated were characterized by narcissin and kaempferol. These findings provided the scientific basis for the improvement of quality evaluation standards for different varieties and offered new insights for the further development of sea buckthorn resources.
研究领域:
植物学
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营养学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 5
·1035
·
IF 4.2
Kaihua Guo
Enzymatic hydrolysis using pectinase is critical for producing high-yield and quality sea buckthorn juice. This study determined the optimal temperature, time, and enzyme dosage combinations to guide manufacturers. A temperature of 60 °C, hydrolysis time of 3 h, and 0.3% enzyme dosage gave 64.1% juice yield—25% higher than without enzymes. Furthermore, monitoring physicochemical properties reveals enzyme impacts on composition. Higher dosages increase soluble solids up to 15% and soluble fiber content by 35% through cell wall breakdown. However, excessive amounts over 0.3% decrease yields. Pectin concentration also declines dose-dependently, falling by 91% at 0.4%, improving juice stability but needing modulation to retain viscosity. Electrochemical fingerprinting successfully differentiates process conditions, offering a rapid quality control tool. Its potential for commercial inline use during enzymatic treatment requires exploration. Overall, connecting optimized parameters to measured effects provides actionable insights for manufacturers to boost yields, determine enzyme impacts on nutrition/functionality, and introduce novel process analytical technology. Further investigations of health properties using these conditions could expand sea buckthorn juice functionality.
研究领域:
食品科学
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酶工程
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Chuan-Guo Ma,Man Zhao,Tian-Lei Si,Xiao-Wei Chen
Sea buckthorn ( Hippophaë rhamnoides L.) pulp oil (SPO) is enriched in natural bioactive components with high pharmacological activities. However, the presence of polysaccharides affects oil quality. Herein, the effects of different polysaccharide-adsorption treatments on the bioactive components and the in vitro antioxidant activities of SPO were investigated. The results demonstrated that the microwave-assisted activated clay (MAE-ACL) adsorption was more effective than the ultrasound-assisted activated clay adsorption for removing polysaccharides. Although the bioactive component levels and antioxidant activities of the SPO were reduced during the adsorption, the squalene content showed no significant difference. By performing principal component and correlation analyses that further evaluated the connection between the bioactive components and the free radical scavenging activities of SPO, MAE-ACL was determined to be the more suitable polysaccharide-adsorption treatment.
研究领域:
食品科学
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植物化学
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产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2023
·
IF 3
Sun Ziyi,Zhang Gaoning,Li Xiaoru,Cui Qingqi,Gong Lili,Tian Zhenhua
This study investigated the chemical and volatile characteristics of sea buckthorn fruits from three different regions in China. The chemical composition of the volatile oil was determined by using a non-targeted gas chromatography and mass spectrometry (GC/MS) method and the differences in chemical composition among the three producing areas were compared by heatmap providing a visual basis for researchers. A total of 93 compounds were identified, including 52 compounds from the Northeast China, 51 from the Xinjiang region, and 37 from Inner Mongolia region. Then, the in vitro antioxidant activity of sea buckthorn fruit oil was measured using DPPH, ABTS, and SOD inhibition tests, and the results showed that sea buckthorn fruit oil in northeast China was the strongest antioxidant, followed by Inner Mongolia and Xinjiang. The results of the CCK-8 experiment indicated that within the tested concentration, there is no cell cytotoxicity of the essential oil in human umbilical vein endothelial cells (HUVECs) cells. The results could supply reference to distinguish sea buckthorn fruit from different production areas and, meanwhile, clarify the activity and safety of sea buckthorn oil.
研究领域:
植物化学
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药理学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Hui Yang,Peter Wilde,Ruijie Wang,Qi Meng,Hongyi Shi,Heng Yu,Zijun Zhou,Jianzhong Han,Weilin Liu
The complex of oat β-glucan (OBG) and flavonoids hampered the digestion of starch-based food and retarded the blood glucose response; however, its effect on gastric emptying and its relative mechanism have not been thoroughly investigated. By using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), antioxidant ability, and enzymic inhibitory tests for the characterization and in vitro semi-dynamic digestion of complexes of OBG (high and low molecular weights) and sea buckthorn flavonoids, we found that the higher molecular weight complex (FU) exhibited stronger ABTS and DPPH radical scavenging abilities and higher α-glucosidase and α-amylase inhibition rates. Mice fed with rice flour with FU addition exhibited the slowest gastric emptying and intestinal propulsion rates and blood glucose rise and had the lowest activity of digestive enzymes and levels of insulin, ghrelin, motilin (MTL), and relevant gene (ghrelin and GHSR mRNA) expression than those in the control and low-molecular-weight groups. This study provided scientific data for the development of foods with delayed gastric rate and hypoglycemic index for specific populations.
研究领域:
食品科学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Dehao Meng,Dongbo Zhao,Zhichao Zhao,Xiaoxue Wang,Yi Wu,Yonghui Li,Zhaolin Lv,Qilin Zhong
To clarify the aromatic compounds of sea buckthorn and their formation pathways, the key aroma compounds in Hippophae rhamnoides subsp. sinensis were determined first. There were 21 compounds identified as the key aroma components (e.g. ethyl isovalerate, ethyl caproate, ethyl octanoate, 1-hexanol, 1-nonanol, phenylethyl alcohol, nonanal, 6-methyl-5-heptene-2-one) of sea buckthorn, which were mainly composed of esters and alcohols. There were obvious differences in the composition of compounds among Hippophae rhamnoides subsp. sinensis (SI, SS) and Hippophae rhamnoides subsp. thibetana (TS). Esters were the main volatiles of Hippophae rhamnoides subsp. sinensis (SI, SS), while alcohols were the main volatiles of Hippophae rhamnoides subsp. thibetana (TS), which resulted in a lack of overall aromas in TS and a strong fruity and winy odor in SI and SS. The aroma of sea buckthorn could be reproduced well by analyzing key aroma components. Additionally, oleic acid, linoleic acid, leucine, phenylalanine, lycopene, and other compounds generated key aroma compounds by fatty acid oxidation pathway, amino acid degradation pathway, mevalonic acid pathway, methylerythritol phosphate pathway, carotenoid degradation pathway. Therefore, the key aroma compounds in sea buckthorn berries and their metabolic pathways were studied in the paper, which provided the research basis for genetic breeding and fine processing of sea buckthorn.
研究领域:
植物学
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代谢组学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2018
·
IF 5.651
Qi Cui,Ju-Zhao Liu,Li-Tao Wang,Yu-Fei Kang,Yao Meng,Jiao Jiao,Yu-Jie Fu
Deep eutectic solvents (DESs) have naturally emerged as an alternative for traditional organic solvents for their efficiency on extracting natural products from plant materials. In the present study, 12 kinds of DESs (choline chloride as the hydrogen bond acceptor) were firstly prepared and applied to extract five main flavonoids (namely rutin, quercetin-3-O-glucoside, quercetin, kaempferol and isorhamnetin) from sea buckthorn leaves (SBL) combined with microwave-assisted extraction. The results showed that the extraction efficiencies of target flavonoids by DES were significantly superior to 70% ethanol. Aiming at obtaining the optimal procedure, the extraction conditions were statistically investigated. Under the optimal conditions in pilot-scale application, the total maximum extraction yields of five main flavonoids reached to 20.820 mg/g, which was 1.321–2.400 folds to those by the traditional extraction methods. Meanwhile, the following enrichment of targets compounds from DES extraction solution exhibited excellent recoveries in the range of 72.36–84.99% were achieved by macroporous resin AB-8. In addition, the enriched flavonoids by AB-8 resin exhibited better antioxidant activities as compared with those unenriched. The results indicated that the potential of the developed method as a promising safe and sustainable alternative for selectively extraction and separation of bioactive substances from plant materials.
研究领域:
植物化学
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天然产物开发
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2023
·
IF 7
Qiaoyun Li,Zuman Dou,Qingfei Duan,Chun Chen,Ruihai Liu,Yueming Jiang,Bao Yang,Xiong Fu
In this study, the structural characters, antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides (HRPs) obtained by the commonly used hot water (HRP-W), pressurized hot water (HRP-H), ultrasonic (HRP-U), acid (HRP-C) and alkali (HRP-A) assisted extraction methods were investigated. The results demonstrated that extraction methods had significant effects on extraction yield, monosaccharide composition, molecular weight, particle size, triple-helical structure, and surface morphology of HRPs except for the major linkage bands. Thermogravimetric analysis showed that HRP-U with filamentous reticular microstructure exhibited better thermal stability. The HRP-A with the lowest molecular weight and highest arabinose content possessed the best antioxidant activities. Moreover, the rheological analysis indicated that HRPs with higher galacturonic acid content and molecular weight showed higher viscosity and stronger crosslinking network (HRP-C, HRP-W and HRP-U), which exhibited stronger bile acid binding capacity. The present findings provide scientific evidence in the preparation technology of sea buckthorn polysaccharides with good antioxidant and bile acid binding capacity which are related to the structure affected by the extraction methods.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Jabeen Farheen,M. Zubair Iqbal,Asim Mushtaq,Yike Hou,Xiangdong Kong
Triple-negative breast cancer (TNBC) is the most common primary tumor of the breast with limited effectual drug availability. Therefore, the aim of the study is to develop an innovative phyto-nanomedicine (PNM) to cure TNBC with the least genotoxicity. Hereinafter, the sea buckthorn’ extracted polyphenols (SBP), combine with metformin (MET), are synthesized as a novel PNM to evaluate its anti-tumor properties, effectiveness, and mechanism of action in TNBC in vitro and in vivo models. The SBP exhibits 16 new kinds of polyphenols that are been reported earlier which regulated cell development, proliferation, and programmed cell death (PCD) effectively. SBP-MET PNM inhibits MDA-MB-231 (47%), MDA-MB-436 (46%), and 4T1 (46%) cell proliferation but does not affect L929 normal murine cell development and successfully induce PCD (73.19%) in MDA-MB-231 cells. Mechanistically, in vivo SBP-MET proteome expression profiling reveals upregulation of proapoptotic Bax protein and activation of Fas signaling pathways convince downstream Daxx and FADD proteins, which further triggers Caspase-3 that prompts apoptosis in human TNBC cells by cleaving PARP-1 protein. Current findings establish innovative highly biocompatible phyto-nanomedicine that has significant potential to inhibit TNBC cell growth and induce regulated cell death (RCD) in vivo model, thereby opening a new arena for TNBC therapy.
研究领域:
肿瘤学
·
药理学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Linze Liu,Yawen Deng,Hangqi Ren,Ying Ren,Taizheng Tang,Eerdun bayaer,Shaobo Li,Tungalag Dong,Xueyan Yun
Plant extracts with antioxidant activity are frequently employed to inhibit acrylamide (AM) production, but there has been limited focus on their inhibitory effects on AM during the extended storage of foods. In this study, impregnation solutions were prepared using Hippophae rhamnoides L (Hp) and Alkekengi officinarum Moench (Ak) fruits extracts to investigate their protective effects on roasted chicken breasts (RCB) during processing and storage (vacuum packed at 4°C and stored at 25°C for 90 days). In a 90-day storage experiment with RCB, these extracts also slowed the increase of AM in RCB. However, these extracts exhibit inadequate protective effects on RCB during long-term storage. It is needed to develop new methods for monitoring and inhibiting AM formation in RCB.
研究领域:
食品科学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）