Frontiers in Immunology
·
2022
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IF 8.786
Cheng Jingliang,Fu Jiewen,Tan Qi,Liu Zhiying,Guo Kan,Zhang Lianmei,He Jiayue,Zhou Baixu,Liu Xiaoyan,Li Dabing,Fu Junjiang
ISG20 inhibits viruses such as SARS-CoV-2 invasion; however, details of its expression and regulation with viral susceptibility remain to be elucidated. The present study analyzed ISG20 expression, isoform information, survival rate, methylation patterns, immune cell infiltration, and COVID-19 outcomes in healthy and cancerous individuals. Cordycepin (CD) and N6, N6-dimethyladenosine (m62A) were used to treat cancer cells for ISG20 expression. We revealed that ISG20 mRNA expression was primarily located in the bone marrow and lymphoid tissues. Interestingly, its expression was significantly increased in 11 different types of cancer, indicating that cancer patients may be less vulnerable to SARS-CoV-2 infection. Among them, higher expression of ISG20 was associated with a long OS in CESC and SKCM, suggesting that ISG20 may be a good marker for both viral prevention and cancer progress. ISG20 promoter methylation was significantly lower in BLCA, READ, and THCA tumor tissues than in the matched normal tissues, while higher in BRCA, LUSC, KIRC, and PAAD. Hypermethylation of ISG20 in KIRC and PAAD tumor tissues was correlated with higher expression of ISG20, suggesting that methylation of ISG20 may not underlie its overexpression. Furthermore, ISG20 expression was significantly correlated with immune infiltration levels, including immune lymphocytes, chemokine, receptors, immunoinhibitors, immunostimulators, and MHC molecules in pan-cancer. STAD exhibited the highest degree of ISG20 mutations; the median progression-free survival time in months for the unaltered group was 61.84, while it was 81.01 in the mutant group. Isoforms ISG20-001 and ISG20−009 showed the same RNase_T domain structure, demonstrating the functional roles in tumorigenesis and SARS-CoV-2 invasion inhibition in cancer patients. Moreover, CD and m62A increase ISG20 expression in various cancer cell lines, implying the antiviral/anti-SARS-CoV-2 therapeutic potential. Altogether, this study highlighted the value of combating cancer by targeting ISG20 during the COVID-19 pandemic, and small molecules extracted from traditional Chinese medicines, such as CD, may have potential as anti-SARS-CoV-2 and anticancer agents by promoting ISG20 expression.
研究领域:
医学
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分子生物学
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肿瘤学
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病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2020
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IF 7.183
Shanshan Zhao,Zhu Tao,Muqiao Han,Yu-xi Huang,Bin Zhao,Liang Wang,Xuelin Tian,Fangang Meng
Conventional hydrophobic membranes are susceptible to membrane fouling and pore wetting, impeding the applications of membrane distillation (MD) processes for water recovery from complex wastewater. To address these challenges, we herein developed a novel Janus membrane by a hybrid plant-derived polyphenols coating and polyelectrolytes layer-by-layer assembly strategy. Through alternative deposition of catechol/chitosan and polyethylenimine (PEI) on top of a hydrophobic polyvinylidene fluoride (PVDF) substrate, a Janus membrane with a superhydrophilic and defect-free top layer was achieved. The synergistic effects of covalent bonding, non-covalent interactions, and electrostatic interactions between neighboring coatings endowed the membrane with robust chemical stability in the pH range of 2–12. In comparison with the pristine PVDF membrane, the Janus membrane exhibited remarkably stable water vapor flux and high quality of effluent during long-term treatments of non-ionic/cationic surfactants and Tween® 20-stabilized oil-in-water (O/W) emulsion in the bench-scale direct contact membrane distillation (DCMD) experiments. The excellent anti-fouling and anti-wetting properties of the Janus membrane were attributed to its hierarchical structured superhydrophilic surface, in which a strong hydration layer was formed and served as an energetic barrier to prevent adsorption of surfactants and oil droplets. Moreover, the electrostatic repulsion between the protonated amino groups on the membrane surface and cationic surfactant molecules also contributed to the robust long-term performance (nearly one-week operation) of the Janus membrane in the treatment of saline water containing cationic surfactant. Our findings reveal the significant role of surface functionality in fouling and wetting resistance of MD membranes and the as-developed Janus membrane shows great potential for water recovery from saline wastewater containing challenging contaminants via DCMD.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2022
·
IF 4.889
Guanghui Zhang,Yan Liu,Chao Chen,Lulu Long,Jinsong He,Dong Tian,Ling Luo,Gang Yang,Xiaohong Zhang,Yanzong Zhang
Superwettability materials with a single function cannot meet the needs of different oil-bearing wastewater separations. This study proposed a preparation method for the swappable superwettability on cotton fabrics. The superhydrophobic fabric surface that was wetted with ethanol transformed from being superhydrophobic/superoleophilic to being superhydrophilic/underwater superoleophobic, and returned to superhydrophobicity after drying. Regardless of whether it is the oil removal process or the water removal process, the flux of the fabric was above 15,300 L·m −2 h −1 , and the separation efficiency was over 98.6%. Although the continuous separation was conducted 50 times, the flux and separation efficiency did not change significantly. The prepared material could exhibit superhydrophobicity after being immersed in solutions of different pH and actual seawater for some time, even after 50 times of sand impact experiments, indicating excellent mechanical durability and chemical stability. Thus, the preparation strategy of the swappable superwettability materials can develop oil–water separation technology.
研究领域:
材料科学
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环境工程
✓ 已验证
产品关联
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
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2020
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IF 3.784
Jiahang Liu,Jian He,Pan Wu,Chao Yang,Wei Jiang
The preparation of superhydrophobic metal surfaces by one-step immersion in fatty acid solutions is simple and convenient, but the effect of the fatty acid type has not been investigated. In this work, Cu meshes were soaked in ethanol solutions of fatty acids with different carbon chain lengths to obtain surfaces with different wettabilities , which varied from superhydrophilicity to superhydrophobicity. The results showed that the contact angle of the surfaces gradually increased from 2.7 ° for the sample prepared with formic acid to 151.8 ° for the sample prepared with myristic acid. The maximum apparent surface energy and solubility parameter were 82.33 mN/m and 65.79 J 1/2 /cm 3/2 for the C 1 -Cu surface, and the lowest were only 6.3 mN/m and 19.91 J 1/2 /cm 3/2 , respectively, for C 14 -Cu. A simulation confirmed that as the carbon chain length of the fatty acid increased, the solubility parameter decreased, and the hydrophobicity increased. One-step immersion enables facile, inexpensive one-step preparation of membrane surfaces with controllable superhydrophobicity for large-scale industrial applications.
研究领域:
材料科学
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表面工程
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2025
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IF 6.9
Xianglong Xiao,Lizhi Zhao,Lixian Wang,Baitao Xu,Guangyue Du,Huiqin An,Hui Ye,Qingping Xin,Yuzhong Zhang
Membrane distillation (MD) is a promising method to produce fresh water by desalination of seawater and saline industrial wastewater. Hydrophobic membranes for MD tend to scaling and fouling during operation. Herein, liquid-infused membrane (LIM) with slippery surface and gating properties is proposed for anti-scaling/fouling MD. The LIM, denoted by PTFE-2-FL, was fabricated by coating a ∼ 2 μm-pore-sized polytetrafluoroethylene (PTFE-2) with dopamine and polyethyleneimine, silica nanoparticles, and fluoride (abbreviated as F), followed by lubricant filling (abbreviated as L). The lubricant capillary-locked in micropores forms a gate that can be reversibly opened for vapor under proper pressure, but prevents water from passing through, thus achieving vapor/water separation with a maximum salt rejection of 99.99 %. Compared with the non-lubricated membrane, PTFE-2-FL exhibits more stable separation performance, indicating the excellent anti-scaling/fouling properties owing to the non-adhesive lubricant surface avoiding contact between contaminants and membrane substrate, especially when treating high-salinity feed (NaCl of 250 g·L −1 ), simulated gypsum solution (NaCl of 35 g·L −1 , Na 2 SO 4 of 20 mM and CaCl 2 of 20 mM), organic compound-contaminated solutions (humic acid or sodium alginate of 500 mg·L −1 ), and inorganic–organic mixed solutions. PTFE-2-FL also has good reusability compared to the irreversible fouling of the hydrophobic membrane in cyclic MD process.
研究领域:
材料科学
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环境工程
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2023
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IF 7.4
Xiang Yan,Caili Dai,Ruoyu Wang,He Liu,Siwei Meng,Xu Jin,Yong Hu,Yining Wu
Countercurrent imbibition of the fracturing fluid is of much significance for enhancing oil production in tight oil reservoirs during the shut-in period after hydraulic fracturing. The addition of enhanced imbibition agents such as surfactants and nanoparticles into the polymer-based fracturing fluid is conducive to oil production. How different types of imbibition agents affect oil recovery and the applicable conditions for different imbibition agents, however, still need to be clarified. In this article, we used low-field nuclear magnetic resonance (NMR) to measure the one-dimensional (1D) oil signal profiles along the imbibition direction during the countercurrent imbibition process and determine the imbibition distance and water saturation of different imbibition agents including polymer, surfactant, nano-silica (NS) and NS-surfactant complexes. Moreover, by measuring oil–water interfacial tension (IFT), and the adhesion forces between alkanes and the hydrophobic surfaces before/after the imbibition agent treatments by atomic force microscope (AFM), the IFT reduction and wettability alteration abilities of these agents were evaluated. After the NS-surfactant complexes treatment, the adhesion force which represents the force required to pull off oil from the rock surface became the lowest (15.81 pN), merely a quarter of that of NS (73.48 pN) and surfactant (70.01 pN), indicating a superior capability of NS-surfactant to improve wettability. The imbibition efficiency of the four systems in terms of imbibition distance and oil recovery was: NS-surfactant complexes: 38.43 mm 13.2 % > nonionic surfactant: 32.15 mm 9.0 % > NS: 25.88 mm 7.6 % > polymer: 17.64 mm 4.9 %. NS-surfactant nanofluid displayed the longest imbibition distance and highest water saturation, thus possessing the highest imbibition oil recovery. Nonionic surfactant with an IFT of 4.19 mN/m could greatly reduce the IFT compared with NS (35.98 mN/m), but the recovery enhancement induced by IFT reduction was not significant. In contrast, the increase in oil recovery by wettability alteration was distinctly higher than that by IFT reduction, as indicated by the recovery difference between NS-surfactant complexes and surfactant. Accordingly, wettability alteration is the primary mechanism to enhance imbibition oil recovery when ultralow IFT cannot be achieved. This research provides guidance on which type of imbibition agent should be selected to improve imbibition efficiency.
研究领域:
石油工程
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材料科学
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化学工程
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
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IF 8.3
Shiping He,Zijie Li,Anhui Yu,Zhiguang Guo
The flexible manipulation of underwater gas bubbles on solid substrates has attracted considerable research interest from scientists in the fields of water electrolysis, bubble microreactions, drug delivery, and heat transfer. Inspired by the oxygen-binding mechanisms of aquatic organisms, scientists have designed a series of interfacial materials for use in collecting gases, detecting and grading bubbles, and conducting microbubble reactions. Aerophilic surfaces are commonly used in underwater bubble manipulation platforms due to their excellent gas-trapping properties. However, during bubble transport, some of the bubbles are retained in the rough structure of the aerophilic surface and cause gas loss, which in the long run reduces the gas transport function. In addition, the aerophilic surface is prone to failure in high-humidity and high-pressure underwater environments. The lubricant-infused surface features an oil layer that remains stable on a rough substrate and is immiscible with water. Additionally, the bubbles are transported over the oil layer without causing losses other than those dissolved in water. These attributes make it more favorable than the aerophilic surface. Inspired by the unique properties of Nepenthes and cactus spines, we developed a patterned slippery surface [patterned lubricant-infused surface (PLIS)] through laser etching and ammonia etching that facilitates the coexistence of superaerophobic and aerophilic surfaces. The PLIS executes bubble capture utilizing a difference in wettability measuring 78°, transports bubbles through Laplace force and buoyancy, and regulates bubble release by restricting the contact area on the PLIS. The PLIS can be prepared rapidly and affordably in just about an hour, and its potential for large-scale production is high. Following tests for shear, acid and alkali resistance, and corrosion resistance, the PLIS exhibited impressive weathering resistance and appears to have potential for application in some extreme environments.
研究领域:
材料科学
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化学工程
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生物工程
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表面化学
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微流控技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
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2022
·
IF 11.322
Chengzong Zeng,Chaofan Ma,Jun Shen
Gallium (Ga)-based liquid metal (LM) paste is deemed to have potential and extensive applications in the thermal management of electronics. However, some non-metallic carbon-based materials such as diamond or carbon fiber (CF) cannot be wetted by Ga, which is difficult to prepare composites using manual stirring without surface modification. Here, the repeated compression method of the forced wetting was successfully used to prepare the Ga-based LM composites containing carbon-based materials without surface modification. New oxide skin of LM will generate during repeating compression. The bonding mechanism between diamond (/CF) and Ga is that Ga oxide is attached to the diamond (/CF) and then being wetted and coated by Ga. Diamond and CF can be separated and recycled from Ga-based paste. A high thermal conductivity value of 129 W m −1 K −1 was obtained by inducing the distribution of CF using diamond particles, in which liquid Ga was the connecting phase. The density of Ga can be reduced by 21% and the thermal conductivity of the Ga-based paste over 100 W m −1 K −1 can be maintained. The successful development of the Ga-based LM/diamond/CF composite indicates that excellent thermal conductivity can be achieved by inducing the distribution of anisotropic particles using isotropic particles.
研究领域:
材料科学
·
热管理技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Limei Tian,Haixu Dou,Yanlong Shao,Yaozhen Yi,Xue Fu,Jie Zhao,Yong Fan,Weihua Ming,Luquan Ren
Controlled liquid adhesion behaviors on super-wetting surfaces have gained significant attention, however, precise controlling of adhesion behavior is extremely hard to realize due to some inherent limitation in surface chemistry or morphology. Herein, we report a silicone-oil-infused magnetic porous polydimethylsiloxane (SMPP) surface with magnetically tunable super-wetting behaviors. Benefiting from the magnetic-field-responsive structure and interconnected porous characteristics, the SMPP can use magnetic field to control the seepage and absorption of silicone oil in the micropore channels, thereby precisely changing the adhesion of the water droplets on the surface. Compared to conventional super-wetting surfaces, this SMPP surface displays reversible in-situ conversion from ultra-high to ultra-low droplet adhesion with sliding angles ranging from 3° to 180° that hold up well even after severe cutting or abrasion. We also demonstrated that this tunable adhesion behavior can be used not only to enhance droplet manipulation in spatiotemporal but also to design unique microfluidic microreactors and antigravity transport devices. We envision that this proof-in-concept surface can serve as an important component of integrated control systems for intelligent manipulators, biochemical reactions, fluid transport, and biomedical analysis.
研究领域:
材料科学
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微流体技术
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Materials Letters
·
2023
·
IF 11.4
Quan Liu,Zhe Sun,Yanhong Duo,Lei Yin,Kongpeng Lv,Ludan Yue,Qian-Fang Meng,Dingmeng Nie,Jiaojuan Chen,Dixian Luo,Li-Ping Liu,Lang Rao
Immune checkpoint blockade has been regarded as a highly promising cancer immunotherapy approach. However, inefficient immune cell infiltration and the immunosuppressive tumor microenvironment (TME) remain significant causes of low clinical response rates. Here, we identified that trivalent chromium irons (Cr3+) could enhance tumor infiltration of immune cells including M1-type tumor-associated macrophages and CD8+ T cells. Mechanistically, this effect is dependent on the expression of macrophage inflammatory protein-1α (MIP-1α) and the PI3K/Akt/mTOR pathway. Moreover, the Cr-based nanoparticles (Cr NPs) exhibited superior photothermal properties under near-infrared laser irradiation, showing great potential in transforming “cold” tumors into “hot” ones. In combination with the PD-1/PD-L1 pathway inhibitor, we demonstrated that the Cr NPs significantly improved anticancer efficacy in mouse models of hepatoma and melanoma by increasing antitumor immune cell infiltration and relieving immunosuppressive TME. Overall, the Cr NPs offer a new dual-functional platform for local and systemic cancer treatments, paving the way toward cancer photometalloimmunotherapy.
研究领域:
肿瘤学
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免疫学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Current Pharmaceutical Analysis
·
2025
·
IF 1.5
Guokan Ge,Xiaohui Jiang,Xiaofeng Tian,Yuan Zhou,Guangxin Cao
Background Previous studies have shown the potential therapeutic effect of Salvia miltiorrhiza on hepatocellular carcinoma (HCC). This study commenced to identify the diagnostic biomarkers and predict the role of Salvia miltiorrhiza in HCC. Methods TCGA-HCC cohort and HCCDB18 dataset were acquired from public database. Chemical components of Salvia miltiorrhiza was identified by the liquid chromatography-tandem mass spectrometry (LC-MS/MS). Differentially expressed genes (DEGs) between tumor and control groups were recognized by “DESeq2” and “Limma” packages. Prognosis-associated DEGs were screened through univariate cox analysis. The compounds-genes network was established using Cytoscape software, and the biomarkers were identified accordingly. Nomogram was constructed employing “rms” package, the diagnostic performance of which was assessed. Immune infiltration was evaluated via “CIBERSORT” and “estimate” packages. Pathways involved in biomarkers were analyzed by GSEA_4.2.2 software. Molecular docking model was constructed applying AutoDocktools software. The cell viability, migration and invasion of HCC cell line treated with β-Estradiol or/and overexpressing AKR1C3 were assessed by Cell Counting Kit-8 (CCK-8), Wound healing and Transwell assays. Results This study identified 57 compounds from Salvia miltiorrhiza and screened 54 poor and 4 good prognostic DEGs shared in TCGA-HCC cohort and HCCDB18 dataset. Further, we established the compounds-genes network of 10 Salvia miltiorrhiza components and 3 prognostic DEGs. Accordingly, AURKB , HDAC11 and AKR1C3 were identified as biomarkers to construct nomogram, displaying good diagnostic performance in HCC. Three biomarkers were notably correlated with most immune cells, and mainly participated in tumor proliferation, cell cycle, apoptosis and energy supply relevant pathways. Molecular docking model indicated that three compounds from Salvia miltiorrhiza (Ethinylestradiol, β-Estradiol, 16alpha-Hydroxyestrone) could stably bind to AKR1C3 . Thereinto, β-Estradiol could inhibit the proliferation, migration and invasion of HCC cells by targeting AKR1C3 . Conclusion This study could lay some foundation for further exploring the pathogenesis of HCC, and provide novel diagnostic biomarkers, treatment targets, and potential management drugs for HCC.
研究领域:
肿瘤学
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药理学
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生物信息学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR AND CELLULAR PROBES
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2025
·
IF 3
Xin Song,Sihao Chen,Junning Cheng,Haiyu Li,Ruixin Wu,Min Yan,Min Wang,Jie Li,Aishun Jin,Wang Wang
Purpose Osteosarcoma (OS) exhibits limited immune cell infiltration that directly contributes to poor prognosis. This study sought to screen and identify pivotal biomarkers of OS immune infiltration and early diagnosis of OS. Methods The immune cell infiltration profiles with transcriptome sequencing data from 88 OS samples were explored with CIBERSORT algorithm. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Protein-protein interaction (PPI) network analyses were applied to identify hub genes, with the expressions confirmed by dual immunofluorescence in 50 OS samples. The new biomarker gene HTRA1 were examined by immunohistochemistry and validated by the Immune score and immune gene expression profile analyses. The impact of HTRA1 on OS prognosis was verified by Least absolute shrinkage and selection operator (LASSO) regression analysis. The biological effect of HTRA1 was characterized in MG63 cells. Result CD8 + T cells, activated memory CD4 + T cells and plasma cells were positively correlated with the prognosis of OS. Hub genes CCL5 , CXCL9 , CXCL13 , and HTRA1 , exhibited positive correlation with the infiltration of both CD8 + T cells and CD4 + T cells. HTRA1 expression was reduced in osteosarcoma tissues, which was positively correlated with immune scores and the expressions of immune-related genes. High levels of HTRA1 were associated with favorable OS prognosis, and could negatively impacted MG63 malignant characteristics. Conclusion CCL5 , CXCL9 , CXCL13, and HTRA1 were OS hub genes positively correlate with CD8 + T cell and CD4 + T cell infiltrations. HTRA1 can serve as an underlying biomarker for the prognosis and immunotherapy of OS.
研究领域:
肿瘤学
·
免疫学
·
生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）