ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2025
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IF 11.3
Kena Zhang,Quanzhi Xiao,Jianbo Jia,Yan Gao,Yanfei Zhou,Xiao-Xia Zhou,Bing Yan
The extensive use of cadmium sulfide nanoparticles (CdS-NPs), along with their natural formation through the complex biogeochemical transformation of anthropogenic cadmium ions (Cd2+), poses substantial risks to ecosystems and human health. Despite this, the mechanisms underlying the toxicity of CdS-NPs remain unclear. A key question is whether their toxicity arises from the nanoparticulate form of cadmium (Cd) or from the release of Cd2+. To explore this, we exposed freshwater clams (Corbicula fluminea) to environmentally relevant concentrations (0.01–1 mg/L) of CdS-NPs or Cd2+ for 10 days. Hematoxylin and eosin (HE) staining revealed significant damage to the digestive gland in both cases. Although CdS-NPs released some Cd2+ (≤10.4%), transcriptomic and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses indicated different toxicity mechanisms. CdS-NPs primarily induce ferroptosis, triggered by lysosomal dysfunction that releases Fe2+ into the cytoplasm, disrupting the cellular iron metabolism. In contrast, Cd2+ primarily induces an autophagic response, as evidenced by the upregulation of autophagy-related markers and activation of apoptosis pathways linked to mitochondrial membrane permeabilization. Overall, our findings suggest that the toxicity of CdS-NPs is not solely derived from Cd2+, highlighting the need to evaluate the risks posed by metal sulfide nanoparticles to benthic ecosystems.
研究领域:
环境毒理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2021
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IF 6.475
Jing-Xuan Gao,Yu-Ying Zhang,Xu-Hui Huang,Rong Liu,Xiu-Ping Dong,Bei-Wei Zhu,Lei Qin
As an important aquaculture shellfish, the superior taste and high nutritional value of oyster ( Crassostrea gigas Thunberg) have drawn extensive attention. In this study, twenty-one free amino acids (FAAs) and six 5′-nucleotides were evaluated through stable isotope labeling-liquid chromatography coupled with tandem mass spectrometry (SIL-LC-MS/MS), and the lipid profile was explored using ultrahigh performance liquid chromatography-Q Exactive HF mass spectrometry (UHPLC-QE/MS). The adductor muscle of the oyster possessed a high level of sweetness-related amino acids (Arg, Gly and Hyp) and 5′-nucleotides. A total of 149 lipid species were detected in different tissues of oysters, including 17 triacylglycerols (TAGs), 6 diacylglycerols (DAGs), 61 phosphatidylcholines (PCs), 29 phosphatidylethanolamines (PEs), 11 lysophosphatidylcholines (LPCs), 8 lysophosphatidylethanolamines (LPEs), and 1 lysophosphatidylinositol (LPI). FAAs, 5′-nucleotides and lipid profile in the digestive gland of oysters can be divided into three stages, from November to April, May to July, and August to October. The highest proportion of umami-taste amino acids and 5′-nucleotides appeared from March to May. The highest percentage of high unsaturation degree glyceride and phospholipids appeared in August and April, respectively. Thus, the results reported in this study are important for product development and sustainable exploitation in the future.
研究领域:
水产养殖
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生物化学
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代谢组学
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营养科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
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2024
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IF 5.3
Jiani Liu,Yueyong Shang,Fujing Deng,Zhihua Feng,Menghong Hu,Youji Wang
In the marine environment, nanoparticles play a role in adsorbing and catalytically degrading organic pollutants, thereby mitigating their toxic effects on aquatic organisms. This study aimed to investigate the impact of nano titanium dioxide (nTiO 2 ) and tris (2-chloropropyl) phosphate (TCPP) on the hemolymph and digestive function of the thick-shell mussel Mytilus coruscus . Mussels were divided into a control group, a group exposed to TCPP alone, a group exposed to a combination of TCPP and 0.5 mg/L nTiO 2 , and a group exposed to a combination of TCPP and 1 mg/L nTiO 2 . After 14 days of exposure, oxidative stress responses, including superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, immune defense responses, including acid phosphatase (ACP) and alkaline phosphatase (AKP) activities, and gene expression, including HSP70 expression, were measured in the hemolymph and digestive glands of the mussels. Compared to the control group, mussels solely exposed to 100 μg/L TCPP exhibited a significant reduction in SOD activity in the hemolymph. When TCPP was co-exposed with 0.5 mg/L nTiO 2 , there were significant increases in MDA content and AKP activity in both the digestive gland and hemolymph compared to the control group. Upon co-exposure of TCPP with 1 mg/L nTiO 2 , MDA content and AKP activity in the digestive gland significantly decreased, while SOD, ACP, and AKP activity in the hemolymph significantly increased and MDA content significantly decreased, returning to the control group levels. Furthermore, in the combined exposure, HSP70 gene expression significantly decreased as the nTiO 2 concentration increased from 0.5 mg/L to 1 mg/L. In summary, TCPP impacted the hemolymph and digestive function of mussels, whereas a concentration of 1 mg/L nTiO 2 effectively alleviated the toxic effects of TCPP. This study is crucial for assessing the ecological risks of nanoparticles and emerging organic pollutants in marine environments, and provides new insights into the interaction between nTiO 2 and TCPP, as well as the influence of nTiO 2 concentration on mitigating TCPP toxicity.
研究领域:
海洋生物学
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环境科学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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