Biology-Basel
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2022
·Vol. 11
·期 4
·503
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IF 5.168
Jingyun Ding,Huiyan Wang,Zequn Li,Jiangnan Sun,Peng Ding,Xiaomei Chi,Mingfang Yang,Yaqing Chang,Chong Zhao
Simple SummarySea urchins are key in the trophic cascades of benthic communities. They also possess physiological characteristics of low digestibility. Thus, we explored the relationship between the indicators (digestive enzymes activities and the gut emptying) and molecules (TRPA1 and 5-HT) related to digestive physiology in the sea urchinStrongylocentrotus intermedius. The results in the present study indicate that digestive enzyme activities and gut emptying are correlated with the TRPA1 and 5-HT in the gut ofS. intermedius.This novel finding not only is important for understanding the low food digestibility of sea urchins but provides an entry point to further investigate the molecular details of digestive physiology of sea urchins.AbstractThe energetic link in the benthic community is based on physiological characteristics of the low food absorption efficiency of sea urchins. Low food absorption efficiency of sea urchins is correlated with the activity of digestive enzymes and the duration of food in their gut. Thus, the digestive enzymes activities (pepsin and amylase enzyme activities) and gut emptying are important indicators in assessing nutrient digestion and absorption in sea urchins. In the present study, the relationship between these indicators and molecules related to digestive physiology were quantified in sea urchins. We found (1) an inter-regulatory relationship existed between Transient receptor potential cation channel, subfamily A, member 1 (TRPA1), and serotonin (5-hydroxytryptamine; 5-HT) in the gut ofStrongylocentrotus intermedius; (2) digestive enzyme activities were negatively correlated with the TRPA1 and concentration of 5-HT in the gut ofS. intermedius; (3) gut emptying rate was positively correlated with TRPA1 and concentration of 5-HT in the gut ofS. intermedius. The present study revealed that the digestion and absorption of food are correlated with the TRPA1 and 5-HT in the gut ofS. intermedius, which provides valuable information about the digestive physiology of sea urchins. This novel finding is relevant to understanding the low food digestibility of sea urchins. It also provides valuable information to the digestive physiology of sea urchins, which are key to maintaining the stability of food webs in the marine ecosystem.Keywords:sea urchin;TRPA1;5-HT;digestive enzyme;gut emptying
研究领域:
海洋生物学
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生理学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
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2018
·
IF 3.185
Yafei Duan,Yun Wang,Jiasong Zhang,Yongxu Sun,Jun Wang
Organic acids acts as an growth promoter and antimicrobial agent in aquaculture. The present study investigated the effects of a natural organic acid - succinic acid (SA) on the growth, digestive enzymes , immune response and resistance to ammonia stress of Litopenaeus vannamei . The shrimps were firstly fed with diets containing different levels of SA: 0% (Control), 0.25% (SA1), 0.50% (SA2), and 1.0% (SA3) ( w / w ) for 56 days, followed by an acute ammonia stress for 48 h. The results indicated that dietary of SA improved the growth of shrimp, and increased the survival rate of shrimp after ammonia stress for 48 h. The amylase , lipase and pepsin activity increased in hepatopancreas in three SA group, while trypsin activity was only increased in the SA1 and SA2 groups. At 56 d, T-NOS activity, proPO and HSP70 gene expression level increased in the three SA group, PO activity increased in the SA1 and SA2 groups, T-AOC content and Toll gene expression level increased in the SA2 and SA3 groups, Trx and SOD gene expression level increased in the SA2 group, while Imd , GS and GDH gene expression level was no changes. After exposure to ammonia stress for 48 h, immune biochemical parameters (T-AOC and PO) and genes ( proPO , HSP70 , Trx and GDH ) expression level increased in the three SA group, T-NOS activity, Toll , Imd and GS gene expression level increased in the SA2 and SA3 groups, while SOD gene expression level increased in the SA1 and SA2 groups. These results indicated that SA improved growth, enhanced digestive and immune capacities of L. vannamei against ammonia stress, and may be a potential feed additive for shrimp. The optimal dietary supplementation dosage is 0.50% ( w / w ) in diet.
研究领域:
水产养殖学
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生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
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2023
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IF 5.6
Tao Gao,Shuang Liu,Huang-yang Luo,Ren Wu,Rui Yan,Ling-zhi He,Qing-yu Nie,Hua-li Tang,Guiju Sun
Chuanminshen violaceum , as a food and folk medicine, is cultivated and grown only in China. Its polysaccharides play an important role in medicinal benefits. Herein, crude C. violaceum polysaccharides (CVPs) were purified to yield two polysaccharide fractions (CVPs-1-G and CVPs-2-G) using DEAE Cellulose-52 and Sephadex G-100 column chromatography. Structural analyses indicated that both CVPs-1-G and CVPs-2-G were glucans with average molecular weights of 8.1 kDa and 187.5 kDa, respectively. The main chain of the major component (CVPs-1-G) might be composed of (1 → 4)-linked α-Glc p , and the branched-chain might be an α-Glc p → 4)-α-D-Glc p -(1 → 6)-α-D-Glc p -(1 → 4)-α-D-Glc p , which connected to the main chain through the O-6 position of → 3,6)-α-D-Glc p -(1 →. In addition, CVPs-1-G displayed ABTS, DPPH, and hydroxyl radical scavenging activity, as well as competitive inhibitory activities of α-amylase and non-competitive inhibitory activities of lipase and pepsin. According to molecular docking, its inhibitory activity on digestive enzyme might be caused by hydrogen bond and hydrophobic interaction between them. Thus, CVPs exhibit strong potential for use in functional foods and pharmaceuticals.
研究领域:
食品科学
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药理学
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植物化学
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DOI
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Jie Yang,Yujing Sun,Xuyan Dong,Man Li,Yang Qin,Lei Dai,Qingjie Sun
This study investigates the interaction of amino-modified starch nanoparticles (NH 2 -SNPs) and unmodified SNPs with pepsin and trypsin and the influence of the formation of protein coronas on the release of polyphenols. We discovered that NH 2 -SNPs bound loosely to pepsin, while they bound tightly to trypsin, by quartz crystal microbalance with dissipation monitoring and zeta potential measurement. SNPs did not easily bind to the two digestive enzymes. In addition, the influence of NH 2 -SNPs on digestive enzymes was investigated by ultraviolet-visible spectrophotometry, and circular dichroism spectroscopy, showing that the addition of NH 2 -SNPs had no effect on the conformational structure of pepsin and trypsin. Using NH 2 -SNPs and SNPs to load four polyphenols revealed that the nanoparticles had a slow-release effect on the polyphenols, but the presence of protein coronas had little effect on the release. The release was mainly related to the destruction of the starch-based carrier by the amylase in digestive enzymes.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
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2016
·Vol. 48
·期 2
·290-302
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IF 0.665
Chenmin Liu,Yuzhe Han,Tongjun Ren,Zhiqiang Jiang,Fuqiang Wang,Mingling Liao,Jing Wang
A 56-d feeding trial was conducted to evaluate the effects of dietary lysine levels on growth performance, intestinal digestive enzymes, and nonspecific immune response of Apostichopus japonicus juveniles. Five semipurified diets were formulated to contain graded levels of lysine (from 0.28 to 2.32% of dry matter). Although no significant differences ( P > 0.05) were observed in survival rate, final weight (FW), and body weight gain (BWG), the FW and BWG increased from 0.28 to 1.19% with increasing dietary lysine levels, then showed a declining tendency. Broken-line regression analysis of BWG indicated sea cucumber requires lysine at 0.76% of dry diet. Proximate composition, lysine content of the body wall, and total amino acid profiles were not affected by dietary treatments. Intestinal protease level increased with increasing dietary lysine level from 0.28 to 1.19%, then decreased. Acid phosphatase and superoxide dismutase activities of the sea cucumbers were not affected by various dietary lysine levels; however, significantly ( P < 0.05) higher alkaline phosphatase activity was observed in sea cucumbers fed 2.32% lysine diet than those fed diets having 0.28 and 1.89% lysine. Overall, sea cucumber juveniles fed with diets containing between 0.76 and 1.19% lysine showed better growth performance, digestive enzymes, and nonspecific immune enzyme activities.
研究领域:
水产养殖学
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营养学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2020
·Vol. 55
·期 9
·3082-3090
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IF 2.773
Yahya Saud Hamed,Mohamed Abdin,Guijie Chen,Hafiz Muhammad Saleem Akhtar,Xiaoxiong Zeng
Summary In an ultrasonic bath and for 30 min in the presence of water as extraction solvent in a ratio of 1:10 (g/mL), effect of extraction temperature on extraction of caffeoylquinic acids (CQAs) from Moringa oleifera leaves (MOL) was investigated. Gradually increasing in extraction temperature caused a growing in mono-CQA and declining in 3,5-diCQA. The extract obtained at 80 °C showed the highest antioxidant capacity, and IC 50 values for scavenging activities on DPPH radicals and ABTS radicals were 56.9 and 53.6 µg/mL, respectively. Compared with α-glucosidase and α-amylase, the extract showed the highest inhibition on pancreatic lipase (IC 50 of 0.073 mg/mL). Increasing the extraction temperature improved cytotoxicity of the extract on cancer cell lines of HeLa and HepG2. Compared with gram-negative Escherichia coli and Salmonella typhimurium , all the extracts showed higher inhibition on the growth of gram-positive Bacillus cereus and Staphylococcus aureus . Our results suggested that use of higher extraction temperature (80 °C) improved the biological activity of MOL extract.
研究领域:
植物化学
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药理学
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食品科学
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DOI
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
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2022
·Vol. 116
·期 1
·181-191
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IF 2.447
Zhao Xu-dong,Geng Yi-shu,Hu Tian-yi,Li Wen-xuan,Liang Ying-ying,Hao De-jun
In China, Hyphantria cunea (Drury) is an invasive phytophagous pest; it attacks nearly all species of defoliated trees. To develop integrated pest management programs (IPM) for H. cunea, we need to ensure the availability of insects by mass-rearing them on artificial diets under laboratory conditions. This study compared the growth characteristics, nutritional indices, growth indices, and digestive enzyme activity of insects reared on Pterocarya stenoptera C.DC (Fagales: Juglandaceae), the Chinese wingnut, and an artificial diet. We also investigated the correlation between diet components and growth indices using principal components analysis and Pearson correlation analysis. We found that mass-rearing of H. cunea on an artificial diet was feasible. It led to a shorter developmental period, with heavier larvae and pupae than natural diets. The principal components analysis indicated that the growth indices and α-Amylase were significantly positively associated with PC1, which explained 82.45% of the total data variability. Pearson correlation analysis showed a significant correlation between digestion, absorption parameters, and growth. Developing a mass-rearing program to produce H. cunea on an artificial diet will be valuable for improving IPM strategies. Understanding the mechanism of the responses of phytophagous insect populations to anthropogenic diet regulation can provide new ideas and methods for pest control.
研究领域:
昆虫学
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害虫管理
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Hui Yang,Peter Wilde,Ruijie Wang,Qi Meng,Hongyi Shi,Heng Yu,Zijun Zhou,Jianzhong Han,Weilin Liu
The complex of oat β-glucan (OBG) and flavonoids hampered the digestion of starch-based food and retarded the blood glucose response; however, its effect on gastric emptying and its relative mechanism have not been thoroughly investigated. By using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), antioxidant ability, and enzymic inhibitory tests for the characterization and in vitro semi-dynamic digestion of complexes of OBG (high and low molecular weights) and sea buckthorn flavonoids, we found that the higher molecular weight complex (FU) exhibited stronger ABTS and DPPH radical scavenging abilities and higher α-glucosidase and α-amylase inhibition rates. Mice fed with rice flour with FU addition exhibited the slowest gastric emptying and intestinal propulsion rates and blood glucose rise and had the lowest activity of digestive enzymes and levels of insulin, ghrelin, motilin (MTL), and relevant gene (ghrelin and GHSR mRNA) expression than those in the control and low-molecular-weight groups. This study provided scientific data for the development of foods with delayed gastric rate and hypoglycemic index for specific populations.
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
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2019
·Vol. 8
·期 8
·274
·
IF 4.52
Lingfeng Wu,Yufeng Liu,Yin Qin,Lu Wang,Zhenqiang Wu
The anti-oxidative phenolic compounds in plant extracts possess multiple pharmacological functions. However, the phenolic characterization and in vitro bio-activities in various parts of raspberry (Rubus idaeusL.) have not been investigated systematically. In the present study, the phenolic profiles of leaves (LE), fruit pulp (FPE), and seed extracts (SE) in raspberry were analyzed by HR-HPLC-ESI-qTOF-MS/MS method, and their antioxidant activities and digestive enzymes inhibitory abilities were also investigated. The molecular docking analysis was used to delineate their inhibition mechanisms toward type II diabetes related digestive enzymes. Regardless of LE, FPE, or SE, 50% methanol was the best solvent for extracting high contents of phenolic compounds, followed by 50% ethanol and 100% methanol. The LE of raspberry displayed the highest total phenolic content (TPC) and total flavonoid content (TFC). A total of nineteen phenolic compounds were identified. The quantitative results showed that gallic acid, ellagic acid, and procyanidin C3 were the major constituents in the three extracts. The various parts extracts of raspberry all exhibited the strong antioxidant activities, especially for LE. Moreover, the powerful inhibitory effects of the three extracts against digestive enzymes (α-glucosidase andα-amylase) were observed. The major phenolic compounds of the three extracts also showed good inhibitory activities of digestive enzyme in a dose-dependent manner. The underlying inhibitory mechanisms of the main phenolic compounds against digestive enzymes were clarified by molecular docking analysis. The present study demonstrated that the various parts of raspberry had strong antioxidant activities and inhibitory effects on digestive enzymes, and can potentially prevent oxidative damage or diabetes-related problems.Keywords:Rubus idaeusL.;phenolic compounds;HPLC-ESI-HR-qTOF-MS/MS;antioxidant activities;digestive enzymes inhibitors;molecular docking analysis
研究领域:
植物化学
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药理学
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营养学
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DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
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2024
·
IF 3
Zhou Jian-Feng,Xu Hai-Xia,Yin Zhong-Ping,Chen Ji-Guang,Zhang Qing-Feng
Quercetin is the most abundant flavonoid found in human diet. In the present study, the effects of quercetin on postprandial hyperglycemia (PPHG) were investigated in two ways. First, the complexes of quercetin and starch were prepared and characterized. Quercetin can occupy the helix of starch and interact with it through secondary bonds. The digestibility of starch decreased with the increasing proportion of quercetin in the complexes. Second, the inhibitory activities of quercetin on α-glucosidase and α-amylase were investigated in both in vitro and in vivo. Quercetin showed very strong inhibitory activity on α-glucosidase, while its inhibitory activity on α-amylase was far weaker than acarbose. Quercetin is the non-competitive inhibitor of α-glucosidase. Fluorescence titration showed that quercetin and α-glucosidase had one binding site and the binding constant lgKa was 3.70. After oral administration of quercetin–starch complexes in rats, the maximum level of postprandial blood glucose was reduced and delayed. Besides, quercetin also improved the PPHG after administration of sucrose and maltose in rats. Hence, it is deduced that the regulation of quercetin on PPHG is the result of its combination effects on starch and digestive enzymes. The complexes of quercetin and starch may be useful for the development of low-glycemic food.
研究领域:
营养学
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药理学
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食品科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2025
·Vol. 60
·期 2
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IF 3.1
Zhao Yingna,Chen Zhendong,Yang Yueyue,Lin Pei,Jiao Aiquan,Jin Zhengyu
Polyphenols, as a bioactive components with both anti-inflammatory and enzyme-inhibitory activities, have significant potential for application in regulating abnormal glucose metabolism diseases. Resveratrol, gallic acid, caffeic acid, quercetin, and catechin were analysed for their stability during simulated digestion, their inhibitory effects on starch-digesting enzymes, and their anti-inflammatory properties, revealing that the degradation of free phenolics was related to the environment of the gastrointestinal tract. There existed a quantitative relationship between the inhibitory activity of polyphenols on starch-digestive enzymes, with resveratrol being second only to acarbose in its overall effect. Treatment of cells with 25 mmol/L D-glucose for 24 hr induced glycositis injury in HBZY-1 cells. Compared to the high-glucose group, 5–100 μmol/L resveratrol exhibited significant inhibitory effects on the secretion of inflammatory factors. These findings provide useful information on the use of polyphenols, especially resveratrol, as food-grade ingredients for the prevention and management of diabetic nephropathy.
研究领域:
食品科学
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生物化学
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药理学
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营养学
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分子生物学
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产品关联
DOI
高影响因子
AQUACULTURE
·
2019
·
IF 3.022
Yafei Duan,Yun Wang,Xian Ding,Dalin Xiong,Jiasong Zhang
Succinate (SA) is the precursor of propionate that influence the intestine physiological function of animals. In this study, we investigated the microbiome , digestive, and immune responses in the intestines of Pacific white shrimp Litopenaeus vannamei fed diets containing different levels of SA: 0 g/kg (Control), 2.5 g/kg (SA1), 5.0 g/kg (SA2), and 10.0 g/kg (SA3) for 56 days. The results showed that dietary SA improved the intestine mucosa morphology, increased the short-chain fatty acids contents, and altered the intestine microbiota composition. Specifically, SA increased the abundances of Proteobacteria and decreased the abundance of Bacteroidetes . The beneficial bacteria including Ruegeria , Lutimonas , Mameliella , and Sphingomonas were enriched in response to dietary SA; these genus might be involved in nutrient metabolism and the degradation of toxic compounds and pollutants . Additionally, dietary SA also improved the activity of digestive enzymes (amylase, lipase , trypsin, and pepsin) and immune enzymes (total antioxidant capacity, phenoloxidase, total nitric oxide synthase , and nitric oxide), and the expression of immune-related genes (prophenoloxidase, anti-lipopolysaccharide factor, lysozyme , heat shock protein 70 , thioredoxin , mucin-1, mucin-2, mucin-5 AC, mucin-5B, and mucin-19) in the intestine of the shrimp. These results revealed that dietary SA had a positive effect on the intestine health of L . vannamei by modulating the microbial composition and SCFA contents, and enhancing the digestive and immune response in the shrimp intestine.
研究领域:
水产养殖
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微生物学
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营养学
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免疫学
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DOI
高影响因子
危险品化学品经营许可证（带存储）