FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Qing Li,Mengyue Xu,Yunyi Yang,Jian Guo,Zhili Wan,Xiaoquan Yang
Novel supramolecular nanofibrils assembled from food-grade saponin glycyrrhizic acid (GA) are effective building blocks to make complex multiphase systems, e.g., emulsion foams. In this work, the effects of different oil phases (castor oil, sunflower oil, dodecane, and limonene) on the formation, stability and structural properties of long-lived emulsion foams prepared by GA nanofibrils (GNs) were investigated. The obtained results showed that soft-solid emulsion foams (4 wt% GNs) can be fabricated, independently of oil phase, and their structural properties, viscoelasticity, and tribological properties can be well tuned by oil phase polarity. Compared to the GNs aqueous foams, the presence of jammed emulsion droplets in the liquid channels and at the surfaces of bubbles can provide a higher bubble stability for emulsion foams. For more polar oil phase (castor oil), GNs showed a higher affinity to the oil–water interface with a lower interfacial tension, thus forming smaller oil droplets and bubbles, which leads to the higher mechanical strength, denser network microstructures, and lower friction coefficients of emulsion foams. However, the limonene foam exhibited weak storage stability and rheological properties, as well as the relatively low lubrication, which may be related to the formation of oil droplet aggregates and clusters induced by the volatility of limonene. GN-based emulsion foams are thermoresponsive, independently of oils, and the temperature-switchable process for the destabilization and regeneration of foams can be controlled and repeated. These emulsion foams based on natural saponin nanofibrils with tunable properties have potential sustainable applications in foods, pharmaceuticals, and personal care products.
研究领域:
食品科学
·
材料科学
·
界面化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Autophagy
·
2024
·
IF 14.6
Na Kang,Jinling Tan,Sisi Yan,Leiying Lin,Qiang Gao
Lipophagy in the yeast Saccharomyces cerevisiae is a microautophagic process in which lipid droplets (LDs) are directly engulfed into the vacuole, despite the fact that multiple core ATG (autophagy related) genes related to general autophagy have been reported to be essential for this process for unknown reasons. In this study, we report new findings about the regulation of lipophagy by analyzing, under different culture conditions, both the engulfment of LDs into the vacuole and the degradation of LD surface proteins. We find that the degradation of LD surface proteins relies on autophagy and can occur independently of lipophagy. Furthermore, glucose restriction can trigger an ATG1-independent lipophagic process, depending on the glucose concentration in the mediums. In summary, we describe an ATG-independent lipophagic process in yeast, such that the overall level of lipophagy in cells is governed by a dynamic balance between the ATG-dependent and -independent lipophagic processes.Abbreviation AP: autophagosome; ATG: autophagy related; CMA: chaperone-mediated autophagy; ESCRT: endosomal sorting complex required for transport; FA: fatty acid; LD: lipid droplet; Ld microdomains: liquid-disordered microdomains; NL: neutral lipid.
研究领域:
细胞生物学
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分子生物学
·
酵母遗传学
✓ 已验证
DOI
PubMed
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Bingnan Wang,Siyuan Wang,Chunyang Li,Jianqing Li,Meixi Yi,Jing-Wen Lyu,Bing Gu,Ryan T.K. Kwok,Jacky W.Y. Lam,Anjun Qin,Ben Zhong Tang
Vacuoles are unique organelles of fungi. The development of probes targeting the vacuoles membrane will enable visualization of physiological processes and precise diagnosis and therapy. Herein, a zwitterionic molecule, MXF-R, comprising of an aggregation-induced emission (AIE) photosensitizing unit and an antibiotic moxifloxacin, was found capable of specifically imaging vacuole membrane and using for targeted antifungal therapy. MXF-R demonstrated a higher signal-to-noise ratio, stronger targeting capability, and better biocompatibility than the commercial probe FM4-64. By using MXF-R, real-time visualization of vacuole formation during Candida albicans ( C. albicans ) proliferation was achieved. More importantly, owing to its varying staining ability towards different fungus, MXF-R could be used to quickly identify C. albicans in mixed strains by fluorescence imaging. Moreover, MXF-R exhibits a remarkable ability to generate reactive oxygen species under white light, effectively eradicating C. albicans by disrupting membrane structure. This antifungal therapy of membrane damage is more effective than clinical drug fluconazole. Therefore, this work not only presents the initial discovery of a probe targeting vacuolar membrane, but also provides a way to develop novel materials to realize integrated diagnosis and therapy.
研究领域:
微生物学
·
医学
·
生物成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Qianqian Guo,Christos Ritzoulis,Jianshe Chen,Jing Xu,Xinmiao Wang
Saliva foam can be easily formed upon different oral movement, but the impact of saliva foam on aroma release and perception is less investigated. Recruited subjects were asked to chew mint-flavor gums to mimic saliva foam generation. During gum-chewing, i.) subjects' retronasal release and dynamic perception of menthol and ii) their salivary bubble number and size, were continuously monitored. In the ‘normal series’ of experiments, bubbles were generated by the oral behavior of mastication; and in the ‘defoamed series’ of experiments, a defoaming agent was simultaneously added to eliminate the bubble effect during mastication. A continuous formation and collapse of saliva bubbles in the normal group led to a stable higher release of menthol in subjects' exhalation and significantly higher mint flavor perception ( p < 0.05). In the defoamed series, saliva bubble numbers were reduced drastically (1–2 orders of magnitude) and more menthol was retained in saliva, which further affected subjects' intensity perception. Multiple physiological factors including salivary protein concentration, viscosity and surface tension contributed to the individual sensory perception variations, and no single parameter could explain the exact relationship between aroma release and retronasal perception. This study provided insights on the aroma delivery in food matrix design and development.
研究领域:
食品科学
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生物学
·
感官科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2016
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IF 4.495
Xu Huanhuan,Wang Ya,Chen Yana,Zhang Pan,Zhao Yi,Huang Yewei,Wang Xuanjun,Sheng Jun
Galloylated catechins, as the main secondary metabolites in the tea plant, including (-)-epigallocatechin-3-gallate and (-)-epicatechin-3-gallate, comprise approximately three-quarters of all the tea plant catechins and have stronger effects than non-galloylated catechins, both on the product quality in tea processing and the pharmacological efficacy to human beings. The subcellular localization of galloylated catechins has been the primary focus of studies that assess biosynthesis and physiological functions. Classical histochemical localization staining reagents can not specifically detect galloylated catechins; thus, their subcellular localization remains controversial. In the present study, we generated a monoclonal antibody (mAb) against galloylated catechins, which can be used for the subcellular localization of galloylated catechins in the tea plant by immunohistochemistry. Direct ELISA and ForteBio Octet Red 96 System assay indicated the mAb could recognize the galloylated catechins with high specificities and affinities. In addition, tea bud was ascertained as the optimal tissue for freezing microtomic sections for immunohistochemistry. What’s more, the high quality mAbs which exhibited excellent binding capability to galloylated catechins were utilized for the visualization of them via immunohistochemistry. Our findings demonstrated that vacuoles were the primary sites of localization of galloylated catechins at the subcellular level.
研究领域:
植物科学
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生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY
·
2022
·Vol. 189
·期 3
·1416-1434
·
IF 8.005
Jiang Dong,He Yilin,Zhou Xiangui,Cao Zhiran,Pang Lei,Zhong Sheng,Jiang Liwen,Li Ruixi
The homotypic fusion and protein sorting (HOPS) complex is a conserved, multi-subunit tethering complex in eukaryotic cells. In yeast and mammalian cells, the HOPS subunit vacuolar protein sorting-associated protein 41 (VPS41) is recruited to late endosomes after Ras-related protein 7 (Rab7) activation and is essential for vacuole fusion. However, whether VPS41 plays conserved roles in plants is not clear. Here, we demonstrate that in the model plant Arabidopsis (Arabidopsis thaliana), VPS41 localizes to distinct condensates in root cells in addition to its reported localization at the tonoplast. The formation of condensates does not rely on the known upstream regulators but depends on VPS41 self-interaction and is essential for vegetative growth regulation. Genetic evidence indicates that VPS41 is required for both homotypic vacuole fusion and cargo sorting from the adaptor protein complex 3, Rab5, and Golgi-independent pathways but is dispensable for the Rab7 cargo inositol transporter 1. We also show that VPS41 has HOPS-independent functions in vacuolar transport. Taken together, our findings indicate that Arabidopsis VPS41 is a unique subunit of the HOPS complex that carries out plant-specific roles in both vacuolar transport and developmental regulation.
研究领域:
植物生物学
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细胞生物学
·
分子遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2025
·
IF 10
Zhiheng Wang,Jingtao Hou,Khurram Shehzad,Shuanglian Xiong,Menghua Cao,Shuxin Tu
Organosilicon exhibits exceptional potential for reducing cadmium (Cd) uptake and accumulation in crops, owing to its molecular structure-endowed high membrane permeability and biocompatibility. However, the current understanding of the mechanisms by which organosilicon mitigates Cd toxicity in rice remains fragmented. We combined controlled hydroponic experiments and foliar organosilicon-sprayed field trials, integrating physiological, biochemical, transcriptomic, histochemical, and in vitro simulation analyses to clarify the underlying mechanisms. Results demonstrated that organosilicon reduces Cd uptake and accumulation and mitigates its toxicity through a synergistic triple mechanism: by reinforcing the root apoplastic barrier via enhanced lignification and suberization, by promoting vacuolar Cd compartmentalization in leaves, and by activating the plant defense system via potentiated glutathione-dependent antioxidant and glyoxalase pathways. Field trials revealed that foliar organosilicon application reduced Cd concentration in rice grains by 48.89 %–52.87 %, decreased Cd-associated health risks by 56.76 %–57.87 %, increased rice yield by 9.26 %–9.98 %, and enhanced grain nutritional quality. This study is the first to unveil the synergistic triple mechanism through which organosilicon mitigates rice Cd toxicity and accumulation. Given its low cost and high efficiency, this approach provides a practical and cost-effective strategy for using silicon-based materials in heavy metal-contaminated agricultural lands.
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Yue Teng,Yan Yang,Zhenjun Wang,Wenjie Guan,Yutong Liu,Hongyan Yu,Luyi Zou
Aspergillus was found to be a vital hyperaccumulation species for heavy metal removal with admirable tolerance capacity. But the potential tolerance mechanism has not been completely studied. This study quantified the amounts of total cadmium (Cd), Cd 2+ , glutathione (GSH), and reactive oxygen species (ROS) in the protoplasts and vacuoles of mycelium. We modulated GSH synthesis using buthionine sulfoximine (BSO) and 2-oxothiazolidine-4-carboxylic acid (OTC) to investigate the subcellular regulatory mechanisms of GSH in the accumulation of Cd. The results confirmed that GSH plays a crucial role in vacuolar compartmentalization under Cd stress. GSH and GSSG as a redox buffer to keep the cellular redox state in balance and GSH as a metal chelating agent to reduce toxicity. When regulating the decreased GSH content with BSO, and increased GSH content with OTC, the system of Cd-GSH-ROS can change accordingly, this also supported that vacuolar compartmentalization is a detoxification strategy that can modulate the transport and storage of substances inside and outside the vacuole reasonably. Interestingly, GSH tended to be distributed in the cytoplasm, the battleground of redox takes place in the cytoplasm but not in the vacuole. These finding potentially has implications for the understanding of tolerance behavior and detoxification mechanisms of cells. In the future bioremediation of Cd in soil, the efficiency of soil remediation can be improved by developing organisms with high GSH production capacity.
研究领域:
微生物学
·
环境科学
·
生物修复
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）