Starch sugars are widely used in various fields, and precise control of their molecular weight may further enhance their application value. This study developed a controllable pore-size enzyme membrane reactor (EMR) to produce starch sugars with target molecular weight. Firstly, polyimide membranes were modified with multiamino compounds to create active sites for subsequent enzyme immobilization. The sieving performance of these modified membranes was analyzed using polyethylene glycol (PEG) of different molecular weights. Next, glutaraldehyde was grafted onto the membrane surface to provide active sites for starch enzyme immobilization. By adjusting the pore size of the EMR, controlled production of starch sugars with specific molecular weights was achieved. Finally, the effects of different operating pressures and flow rates on the sugar production efficiency of the EMR were investigated. This study not only provides guidance for the controlled production of starch sugars but also offers insights into the controllable preparation of EMRs.
Yu-Fan Zhan,Zhuo-Hao Meng,Cheng-Hai Yan,Min Tan,Marriam Khurshid,Yi-Jiangcheng Li,Shao-Jun Zheng,Jun Wang
Enzymatically modified isoquercitrin (EMIQ) is a glyco-chemically modified flavonoid exhibiting notably high biological activity, such as antioxidant, anti-inflammatory and anti-allergic properties. However, the utilization of expensive substrates such as isoquercitrin and cyclodextrin in the conventional approach has hindered the industrial-scale production of EMIQ due to high cost and low yields. Hence, the development of a cost-effective and efficient method is crucial for the biological synthesis of EMIQ. In this study, a natural cascade catalytic reaction system was constructed with α -L-rhamnosidase and amylosucrase using the inexpensive substrates rutin and sucrose. Additionally, a novel approach integrating gradient temperature regulation into biological cascade reactions was implemented. Under the optimal conditions, the rutin conversion reached a remarkable 95.39% at 24 h. Meanwhile, the productivity of quercetin-3- O -tetraglucoside with the best bioavailability reached an impressive 41.69%. This study presents promising prospects for future mass production of EMIQ directly prepared from rutin and sucrose.
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