ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Congzheng Zhu,Jin Li,Xinru Chen,Longwen Fu,Zhiyang Zhang,Yunqing Wang,Xiaoyan Wang,Lingxin Chen
CXC chemokine receptor 4 (CXCR4) is recently regarded as a valuable biomarker for triple-negative breast cancer (TNBC) metastasis but lacks available imaging reagents. Surface-enhanced resonance Raman scattering (SERRS) with resonant dyes has emerged as a powerful tool for single-cell imaging because of the electronically enhanced vibrational fingerprint signals. However, resonant Raman signals are often overwhelmed by accompanying fluorescence backgrounds. To address this, two black hole quenchers (BHQs) are designed as visible resonance Raman reporters with absolutely nonfluorescent readouts. Ultrafast spectroscopy elucidates that the nonfluorescent mechanism of the reporters originates from the ultrafast internal conversion at the subpicosecond scale that quenches the excited states of fluorescence. SERRS nanoprobes (NPs) decorated with such reporters exhibit strong Raman enhancement (5.82 × 10 6 ), the femtomolar-level limit of detection as well as unrivaled photostability (τ s = 26516 s), outperforming that of crystal violet-decorated counterparts. When conjugation of a CXCR4 antagonist, these fluorescence-free SERRS NPs allow for photostable imaging of CXCR4 on TNBC cells at the single-cell level, and for monitoring the expression variation during combined drug treatment. To the best of the available knowledge, this is the first example of absolutely nonfluorescent Raman reporters for single-cell SERRS imaging.
研究领域:
生物医学工程
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癌症研究
·
分子影像学
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DOI
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Analytical Methods
·
2023
·Vol. 16
·期 4
·576-582
·
IF 3.1
Fangfang Yang,Shuang Li,Jialiang Wu,Shufeng Liu
Reversible structural changes in DNA nanomachines have great potential in the field of bioanalysis. Here, we demonstrate an assembly strategy for quencher-free and tunable DNA tweezers based on 2-aminopurine (2-AP), avoiding the tedious fluorescence labelling step. The conformational state of the tweezers could be controlled by specific oligonucleotides (fuel or anti-fuel). Taking advantage of the local environmental sensitivity of 2-AP, the structural changes of the tweezers were easily tracked, and multiple cyclic switching of the tweezers between the open and closed states was achieved. In addition, the influence of oligonucleotide structure on the fluorescence properties of 2-AP was deeply explored. We figured out that the fluorescence of 2-AP was highly quenched by the base-stacking of natural bases in DNA oligonucleotides. Moreover, by comprehensively regulating the type of bases surrounding the inserted 2-AP site, a sensitive fluorescence response towards dynamic change can be obtained. This principle of quencher-free nanodevices based on 2-AP provides a convenient method for monitoring the structural changes of DNA nanomachines.
研究领域:
生物化学
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纳米技术
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分子生物学
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Jincai Yang,Mingzhu Liu,Jin Wu,Tianyi Ma,Yanxuan Li,Yue Zhang,Jingran Sun,Xiaoli Li,Yanjun Fang,Yonghui Wang,Lingchao Cai,Yuan Peng,Zunquan Zhao,Jialei Bai
Antibiotics are emerging hazardous small molecules, requiring urgent need for fast signal-on analytical methods to control antibiotic abuse. Signal-on fluorescence sensing strategies utilizing nanoquenchers and aptamers are fascinating but rarely accomplished on paper-based platforms. Here, a novel multilayer MXene sensing platform was established by leveraging Nb 2 C-MXene as a multilayer nanoquencher and zero-dimensional carbon dots-labeled aptamer (B-CDs@Apt) as a stable and bright recognition probe. The Nb 2 C-MXene has a multilayer nanosheet stack-like structure and efficient mass transfer channels. It can efficiently adsorb abundant B-CDs@Apt probes and quench their fluorescence. Importantly, the Nb 2 C-MXene/B-CDs@Apt system can release the B-CDs@Apt in response to the analyte with high sensitivity, thereby restoring the fluorescent signal. The developed aptasensor achieved sensitive and selective detection of chloramphenicol (CAP) and showed satisfactory anti-interference ability, stability, and practicability. Notably, the multilayer Nb 2 C-MXene/B-CDs@Apt system was successfully transferred to a paper-based sensing platform, with a low-density distribution of multilayer nanoquenchers carrying sufficient aptamer probes for analyte access. In comparison, the monolayer Nb 2 C nanosheets were unable to adsorb enough probes to output analyte-induced signals. The established paper-based analytical device (PAD) showed a LOD of 0.360 ng mL −1 for CAP, which is the first paper-based MXene aptasensor reported for fluorescence detection. By replacing the aptamer and carbon dot, the strategy was further extended to detect another analyte oxytetracycline (OTC), with LODs of 0.399 in tube and 0.867 ng mL −1 on PAD, respectively. Furthermore, concurrent detection of CAP and OTC was achieved using a dual-color PAD, demonstrating the potential to meet multi-target analytical requirements.
研究领域:
分析化学
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生物传感
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环境科学
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 21
·4951-4958
·
IF 3.431
Zhu Yu-Feng,Wang Yong-Sheng,Zhou Bin,Yu Jun-Hui,Peng Li-Lan,Huang Yan-Qin,Li Xue-Jiao,Chen Si-Han,Tang Xian,Wang Xiao-Feng
We report a highly sensitive and selective strategy for Cd(II) assay using a singly labeled multifunctional probe consisting of a Cd(II)-specific aptamer (CAP), which acted as a recognition element for Cd(II) and a signal reporter. The presence of Cd(II) can induce the conformational switching of the CAP, accompanied by a change in fluorescence intensity. Thereby, a fluorescence strategy for Cd(II) assay was established. The proposed method has a detection limit of 2.15 nM, which is much lower than the detection limits reported in related literature. This strategy involves only an aptamer probe, and the use of such a G 4 -based quencher avoids the dual labeling of the CAP with fluorophore/quencher units. It is obviously more convenient and economical than the other aptamer-based biosensors for Cd(II) detection. The mechanism by which Cd(II) induces the CAP to change from a random coil sequence to a stem-loop structure was studied in a series of control experiments. This strategy would be helpful in the design of a sensitive analytical platform for various target assays in environmental and biomedical fields.
The presence of Cd 2+ leads to the conformational change of CAP from a random coil sequence to a stem-loop structure, resulting in a quenching in the fluorescence
研究领域:
分析化学
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环境科学
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生物技术
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生物传感器
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xuhan Huang,Gan Zhang,Zhipeng Fang,Xiaocui Lai,Xiaoyue Xiao,Juan Peng,Weihua Lai
The fluorescence-quenching lateral flow immunoassay (FQ-LFIA) with a “turn on” pattern has shown considerable superiority comparing with traditional LFIA . For sensitive FQ-LFIA, the synthesis of fluorescence quenchers exhibiting excellent optical properties is crucial. Herein, using nanomodification engineering, we synthesized APDCOF, which exhibited intense absorption ( ε 390 and ε 630 = 15.22 and 15.97 mL mg −1 cm −1 , respectively). APDCOF as a “specific” fluorescence quencher, for which its absorption peaks highly coincide with the excitation and emission peaks of the fluorescent microsphere, could occur both the primary and secondary internal filtering effects in response to excitation and emission light, respectively, emerging high quenching efficiency to orange aggregation-induced emission fluorescent microspheres. We employed APDCOF as a quencher to develop APDCOF-LFIA for detecting the carbamate pesticide carbofuran. Compared with a colloidal-gold-based colorimetric LFIA , APDCOF-FQ-LFIA demonstrated 50- and 5.3-fold improvements in the sensitivity of the naked-eye qualitative interpretation and quantitative detection, respectively. Overall, our study presented an efficient and “specific” fluorescence quencher prepared through nanomodification engineering, offering a perspective strategy for constructing high-performance reporters in LFIA .
研究领域:
生物化学
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分析化学
·
材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 11
·6326
·
IF 6.208
Ting Sun,Xian Li,Xiaochuan Jin,Ziyi Wu,Xiachao Chen,Jieqiong Qiu
Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg2+detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg2+–T when Hg2+is present, and no fluorescence is observed. It is digested by exonuclease I without Hg2+, and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg2+concentration in the range of 0–250 nM (R2= 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg2+detection, obtaining a recovery rate from 99.6% to 101.1%.Keywords:positively charged graphene oxide (pGO);exonuclease I;fluorescence quencher;hairpin structure;T–Hg2+–T
研究领域:
生物化学
·
环境科学
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Yunlian Zhang,Rui Liu,Md Mehedi Hassan,Huanhuan Li,Qin Ouyang,Quansheng Chen
Kanamycin (KAN) is widely used in animal husbandry to treat bacterial infections. However, excessive KAN may cause residues and be transmitted to humans and the environment, causing serious adverse effects on humans. Herein, a simple fluorescence resonance energy transfer (FRET)-based aptasensor has been developed for sensitive detection of KAN in food. In the absence of KAN, UCNPs-aptamer hybridized with BHQ3-cDNA and quenched fluorescence was observed due to the FRET effect between BHQ3 and UCNPs. In the presence of KAN, double strands separated and the fluorescence intensity was recovered. Additionally, a linear relation (R 2 = 0.9926) was found in the range of 0.05–50 μM and the recovered fluorescence intensity at 654 nm with a detection limit of 18.9 nM. The method was verified by standard recovery method and HPLC with satisfactory recovery rate (87.0–109.6%) and accuracy (P > 0.05). These results showed the proposed method could be successfully applied to detect KAN in food samples.
研究领域:
食品科学
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分析化学
·
生物技术
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DOI
PubMed
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY A
·
2017
·
IF 2.847
Tao Wang,Ziye Wu,Wei Sun,Shengye Jin,Xingyuan Zhang,Chuanyao Zhou,Jun Jiang,Yi Luo,Guoqing Zhang
We report the formation of macroscopic wires up to centimeters in length from a series of structurally flexible, covalently tethered small-molecular fluorophore-quencher dyads (FQDs, average MW = 425 Da), comprised of carbazole, melatonin, and cyanobenzoate moieties. These FQDs are nonemissive in organic solutions but become moderately to highly luminescent (ΦF = 0.037–0.39) upon formation of wires with emission maxima in the blue region (446–483 nm). The blue photoluminescence (PL) is ascribed to a combination of singlet charge transfer, localized triplet state, and possibly delayed fluorescence emissions with intrinsic luminescence lifetimes ranging from 0.228 to 21333 μs, based on luminescence, transient absorption measurements, X-ray diffraction, and calculations.
研究领域:
材料科学
·
物理化学
·
光电子学
·
纳米技术
·
有机电子学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Mingliang Mei,Lixuan Mu,Sen Liang,Yuan Wang,Guangwei She,Wensheng Shi
The aptasensor , developed from the aptamer, has aroused wide concern in recent years owing to its high sensitivity and specificity. However, the quenching unit involved in the most of the aptasensors is indispensable to the fabrication of an aptasensor, which would undoubtedly increase the complexity of the device. In this study, a facile strategy was developed for construction of the quencher-free aptasensors, in which the quenching units can be omitted, and only an aptamer strand and a fluorophore are necessary. Distinguishable from the configuration of the traditional ones, the aptasensors developed in this work rationally employed the intrinsic quenching abilities of the analytes to directly regulate the fluorescence of the fluorophore. Furthermore, the aptamer strand as a discriminatory unit efficiently captured the corresponding analytes to around the fluorophores. As a result, the fluorescence of the aptasensor can be significantly sensitive to the analytes. The generality of the current design is evidenced by the successful fabrication of seven quencher-free aptasensors for Cu 2+ , Ag + , Hg 2+ , ATP and dopamine through 6-FAM labeling aptamers of Cu 2+ , Ag + , Hg 2+ , ATP, dopamine, 5-TAMRA and ROX labeling aptamers of Cu 2+ . Present strategy endows an aptasensor with a simple structure, high selectivity and fine sensitivity. The configuration of the quencher-free aptasensors fabricated in this work can be readily utilized for more aptasensors.
研究领域:
分析化学
·
生物传感器
·
分子生物学
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PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 17
·4163-4170
·
IF 3.864
Xiaoli Qi,Hui Hu,Yuesuo Yang,Yunxian Piao
A graphite nanoparticle (GN) with a spherical shape and stacked by a few layered graphene sheets is an ideal fluorescent nanoquencher for the fluorophore. We developed a novel aptasensor based on fluorescence resonance energy transfer for the sensitive and specific detection of 17β-estradiol (E2) by using the GN as a fluorescent nanoquencher and the shorter E2 specific aptamer as the sensing probe. The physiological and chemical properties of the aptasensor in response to the E2 capture were investigated with an atomic force microscopic analysis and an E2 detection principle was clarified accordingly. Also, it was demonstrated that the sensitivity of the aptasensor was affected by the length of aptamer and the particle size of the nanoquencher, and the highest sensitivity for E2 detection was achieved with a shorter aptamer of 35 base sequences and a smaller GN with a particle size of around 5 nm. And the detection limit was 1.02 ng mL−1. Moreover, this presented no cross reaction with E2 analogs and was successfully utilized for the real environmental water monitoring.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物传感器
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Saijiao Yang,Meiying Liu,Fengjie Deng,Liucheng Mao,Yiyang Yuan,Hongye Huang,Junyu Chen,Liangji Liu,Xiaoyong Zhang,Yen Wei
Highly sensitive and selective detection of specific DNA or metal ions based on sensor platform of dye-labeled single-stranded DNA (named as ssDNA) and functional materials has attracted great research interest over the past few decades. Herein, we reported for the first time that polydopamine (PDA) modified SiO 2 nanoparticles (PDA- co -SiO 2 NPs) could be severed as effective fluorescent quenchers for fabrication of fluorescent DNA sensor systems for detection of functional nuclear acids and Ag + . To prepare PDA- co -SiO 2 NPs, we just need mix SiO 2 nanoparticles with dopamine in aqueous solution under rather mild experimental conditions. These PDA- co -SiO 2 NPs-based sensor systems possess excellent sensitivity and selectivity, fast response for detection of target ssDNA and Ag + based on different fluorescence quenching effect of PDA- co -SiO 2 NPs towards ssDNA and dsDNA. Furthermore, the high fluorescence quenching ability of PDA- co -SiO 2 NPs allows quantitative detection of target ssDNA with a low detection limit of 1 nM. More importantly, this method would also be easily extended for preparation of many other PDA-based materials for fabrication of fluorescent DNA sensors owing to the universal attachment of PDA with various materials and surfaces. Therefore, this work will open up a new direction for analytical detection of PDA-based functional materials.
研究领域:
纳米技术
·
分析化学
·
材料科学
·
生物技术
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Jingjing Han,Yangbao Miao,Jing Cuan,Hui Zhou,Shihao Zhu,Zixian Wu,You Zhou
The reporter–spacer–receptor (RSR) approach is prevalent to develop molecular turn-on sensors. However, the fluorescent RSR sensors barely operate in solid state, which hinders their fabrication into devices for practical applications. Herein, we present a novel strategy to achieve solid-state luminescence turn-on sensing by assembling RSR architectures within MOF frameworks. Unlike the regular RSR systems, the framework-confined fluorophore and receptor are well arranged and separated even in the solid state. This concept is illustrated by a multicomponent MOF (Fc@NU-1000), which contains organic linkers with a highly luminescent pyrene core as the reporter, Zr6 nodes with unsaturated sites as the receptor, and the incorporated Fc molecules as the quencher. The separate incorporation of pyrene core and Fc in the multicomponent MOF favors an efficient pseudointramolecular photoinduced electron transfer (PET) process, resulting in significant luminescence quenching. Interestingly, such PET process can be blocked via the quencher displacement initiated by the phosphate analyte, therefore recovering the solid-state luminescence of MOF microcrystals. We found that Fc@NU-1000 is shown as a sensitive solid-state luminescence turn-on probe for phosphate with the naked-eye response at a low content. What’s more, this study is the first example of confining a quencher displacement-based RSR system in the MOF framework for solid-state luminescence turn-on sensing, thus also providing new opportunities for MOF materials to develop luminescence turn-on sensors.
研究领域:
材料科学
·
化学传感器
·
分子工程
·
分析化学
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DOI
PubMed
高影响因子
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