PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2017
·Vol. 34
·期 6
·1700035
·
IF 4.474
Haixia Liu,Junying Wang,Yaqi Jing,Jiang Yang,Xueting Bai,Xiaoyu Mu,Fujuan Xu,Xuhui Xue,Lingfang Liu,Yuan-Ming Sun,Qiang Liu,Haitao Dai,Changlong Liu,Xiao-Dong Zhang
High-energy ionizing radiation is widely used in medical diagnosis and cancer radiation therapy. However, high-energy radiation can also impose significant damages in healthy tissues during medical treatments via direct DNA damages and indirect damages from production of reactive oxygen species (ROS). Therefore, it is urgent to develop highly effective radioprotectants with low toxicities that can meet the increasing needs for alleviating the adverse effects from cancer radiation therapy and nuclear emergency. In this work, strongly catalytic ultrasmall (sub-5 nm) cysteine-protected WSe 2 dots are employed to protect healthy tissues against radiation via diminishing radiation-induced free radicals. The WSe 2 dots with high surface activities can recover radiation-induced DNA damages and eliminate the excessive ROS generated from radiation. In vivo experiments confirm that the survival rate of mice treated with WSe 2 dots is significantly elevated with radiation damages postponed under exposure to high-dose ionizing radiation. Furthermore, the free radicals in major organs and hematological system can be appreciably omitted, suggesting their unique role as free radical scavengers. These WSe 2 dots in ultrasmall size show rapid renal clearance of ≈74% injection dose via urine excretion in 24 h and do not cause any apparent toxicity in vivo for up to 30 d.
研究领域:
生物医学工程
·
纳米医学
·
放射生物学
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DOI
高影响因子
PHYTOCHEMISTRY
·
2024
·
IF 3.2
Meihong Liu,Haibo Tan,Haihui Xie
ABSTRACT Chemical investigation on the leaves of Michelia champaca L. (Magnoliaceae) led to the isolation of five previously undescribed phenylethanoid glycosides (PhGs), 4- O - β - d -glucopyranosyl-acteoside ( 1 ), 4"'- O -(6- O - E -caffeoyl)- β - d -glucopyranosyl-acteoside ( 2 ), 4"'- O -(6- O - E -caffeoyl)- β - d -glucopyranosyl-isoacteoside ( 3 ), 6""- O - E -feruloyl-echinacoside ( 4 ), and 6""- O - p - E -coumaroyl-echinacoside ( 5 ), together with eighteen known PhGs. Their structures were determined by spectroscopic and chemical methods. All the known PhGs except acteoside ( 8 ) were not previously reported in the genus. Twenty-one PhGs exhibited more potent DPPH radical scavenging activity and FRAP than l -ascorbic acid ( l -AA), and twenty-two PhGs showed better ABTS radical cation scavenging activity than l -AA. In addition, twelve PhGs displayed more potent cellular reactive oxygen species (ROS) scavenging activity than curcumin. The results revealed that the leaves of M. champaca are a rich source of phenylethanoid glycosides and antioxidants.
研究领域:
药用植物学
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天然产物化学
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植物化学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 3
·606
·
IF 7
Ziyi Yang,Jiaming Cai,Evans Frimpong Boateng,Lujuan Xing,Wangang Zhang
This present study aimed to investigate the effects of various cooking times (1 h, 1.5 h, 2 h, 2.5 h, named as JHBP-1, JHBP-1.5, JHBP-2, JHBP-2.5) on the antioxidant activity and peptide profile of Jinhua ham broth peptides (JHBP). The peptides extracted from uncooked ham were used as an uncooked group with the name of JHBP-0. The results revealed that the antioxidant efficacy in the four cooked groups changed dramatically compared to JHBP-0. After cooking, the DPPH radical scavenging activity, hydroxyl radical scavenging activity and superoxide anion radical scavenging activity decreased, except for the Fe2+chelation and ABTS+scavenging capacity which increased significantly. However, the cooked groups still showed a strong antioxidant capacity. In particular, the superoxide anion radical scavenging ability and the Fe2+chelation action were significantly stronger compared to glutathione (GSH) and butylated hydroxytoluene (BHT) (p< 0.05). JHBP-1.5 also displayed stronger antioxidant capacity than the other three cooked groups, and its secondary structure and mass distribution changed significantly after cooking, specifically with an increased proportion of helix and <1 kDa peptides. Moreover, the constitution of free amino acids (FAAs) and the types of peptides released in the broth increased significantly with a longer cooking time. In total, 1306 (JHBP-0), 1352 (JHBP-1), 1431 (JHBP-1.5), 1500 (JHBP-2), and 1556 (JHBP-2.5) peptide sequences were detected using LC-MC/MC. The proportion of <1 kDa peptides also gradually increased as the cooking time extended, which is consistent with the molecular weight distribution measurements.Keywords:Jinhua ham;broth;bioactive peptides;antioxidant activity;peptide profile
研究领域:
食品科学
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生物化学
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营养学
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产品关联
DOI
PubMed
高影响因子
Biomedical Optics Express
·
2023
·Vol. 14
·期 6
·3003-3017
·
IF 3.4
Tingting Yu,Tingting Yu,Xiang Zhong,Xiang Zhong,Qihang Yang,Qihang Yang,Chao Gao,Chao Gao,Wenyue Chen,Wenyue Chen,Xiang Liu,Xiang Liu,Zhang Liu,Zhang Liu,Tingting Zhu,Tingting Zhu,Dongyu Li,Dongyu Li,Peng Fei,Peng Fei,Zaozao Chen,Zaozao Chen,Zhongze Gu,Zhongze Gu,Dan Zhu,Dan Zhu
Three-dimensional (3D) cell cultures provide an important model for various biological studies by bridging the gap between two-dimensional (2D) cell cultures and animal tissues. Microfluidics has recently provided controllable platforms for handling and analyzing 3D cell cultures. However, on-chip imaging of 3D cell cultures within microfluidic devices is hindered by the inherent high scattering of 3D tissues. Tissue optical clearing techniques have been used to address this concern but remain limited to fixed samples. As such, there is still a need for an on-chip clearing method for imaging live 3D cell cultures. Here, to achieve on-chip clearing for live imaging of 3D cell cultures, we conceived a simple microfluidic device by integrating a U-shaped concave for culture, parallel channels with micropillars, and differentiated surface treatment to enable on-chip 3D cell culture, clearing, and live imaging with minimal disturbance. The on-chip tissue clearing increased the imaging performance of live 3D spheroids with no influence on cell viability or spheroid proliferation and demonstrated robust compatibility with several commonly used cell probes. It allowed dynamic tracking of lysosomes in live tumor spheroids and enabled quantitative analysis of their motility in the deeper layer. Our proposed method of on-chip clearing for live imaging of 3D cell cultures provides an alternative for dynamic monitoring of deep tissue on a microfluidic device and has the potential to be used in 3D culture-based assays for high-throughput applications.
研究领域:
生物医学工程
·
细胞生物学
·
显微成像技术
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产品关联
DOI
PubMed
高影响因子
Phytochemistry Letters
·
2014
·
IF 1.542
Guoyong Luo,Min Zhou,Yan Liu,Qi Ye,Jian Gu,Tianfang Huang,Guolin Zhang,Yinggang Luo
Two new 3-formyl-2-arylbenzofurans, iteafuranals A and B, were isolated from the 95% EtOH extract of the aerial parts of Itea ilicifolia . Their structures were elucidated as ( E )-2-(3,4-dihydroxyphenyl)-3-formyl-4-hydroxy-5-(prop-1-en-1-yl)benzofuran ( 1 , iteafuranal A) and ( E )-2-(3-hydroxy-4-methoxyphenyl)-3-formyl-4-hydroxy-5-(prop-1-en-1-yl)benzofuran ( 2 , iteafuranal B) by comprehensive analyses of spectroscopic data including 1- and 2-D NMR, UV, IR, and HRMS data. The structure of 1 was confirmed by X-ray crystallography analysis. Compound 1 showed DPPH free radical scavenging capacity with an IC 50 value of 0.34 mg/mL. Compounds 1 and 2 showed ABTS cation radical scavenging capacity with IC 50 values of 0.23 and 0.30 mg/mL, respectively.
研究领域:
植物化学
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药理学
·
天然产物研究
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DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2013
·
IF 3.01
Jianguo Cao,Xian Xia,Xiling Dai,Jianbo Xiao,Quanxi Wang,K. Andrae-Marobela,H. Okatch
The profiles and bioactivities of flavonoids extracted from Dryoathyrium boryanum (Willd.) Ching were investigated. The total flavonoids content in extract from D. boryanum is about 145.8 mg/g. By means of HPLC–DAD–ESI–MS, the main flavonoids in D. boryanum were tentatively identified as 3-hydroxyphloretin 6′-O-hexoside, quercetin-7-hexoside, apigenin7-O-glucoside, luteolin 7-O-glucoside, apigenin 7-O-galactoside, acacetin 7-O-(α-D-apio-furanosyl) (1 → 6)-β- d -glucoside, 3-hydroxy phloretin 6-O-hexoside, luteolin-6- C -glucoside. 0.21 mg/ml flavonoids extract from D. boryanum showed very strong superoxide anion radical scavenging potential, which is higher than that of rutin (0.25 mg/ml). The extract (0.21 mg/ml of flavonoids) from D. boryanum exhibited similar DPPH scavenging potential with that of rutin (0.25 mg/ml). However, rutin (0.25 mg/ml) showed a significantly higher reducing power and ABTS scavenging potential than that of 0.21 mg/ml flavonoids extract from D. boryanum . It had no effect on acetylcholinesterase. D. boryanum can be considered as a medicinal plant and the flavonoids from D. boryanum are excellent antioxidants.
研究领域:
植物学
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药理学
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2014
·Vol. 9
·期 3
·e93000
·
IF 3.534
Hao Cai,Zhiyong Xie,Guanghui Liu,Xiuman Sun,Guangtian Peng,Baoqin Lin,Qiongfeng Liao
Reactive oxygen species leads to some diseases associated with oxidative stress. Callicarpa kwangtungensis Chun (CK) is a common remedy in traditional Chinese medicine and possesses diverse biological activities involving antioxidant properties; its main compounds phenylethanoid glycosides (PG) and flavonoids are always reported as antioxidants. In order to develop CK as a safe and activated antioxidant, our investigation was performed to validate antioxidant properties and assess which types of compounds (similar polarity or similar structure), even which compounds, played the role of antioxidants. The extracted compounds of CK were analyzed qualitatively and quantitatively by HPLC-DAD-ESI-Trap MS and UV for their contents and antioxidant activities. The correlations between antioxidant activities and known contents were respectively counted and a semi-quantitative experiment was designed to screen antioxidant compounds of CK with HPLC-UV. The n-butanol fraction (BF) showed the highest total phenolic and flavonoid contents (TPC, TFC), and three PG (forsythiaside B, poliumoside and acteoside) contents. BF showed the significantly best ( P <0.05) activities in most assays. There were significant correlations ( P <0.05) between DPPH•, ABTS + •, •O 2 − scavenging, Cu 2+ -chelating, anti-lipidperoxidation activities and TPC. BF also has significant antioxidant activities on CCl 4 -induced acute liver injury Mice and TBHP-reduced HepG2 cells. Nine PG (forsythiaside B, poliumoside, acteoside, alyssonoside, brandioside and their derivatives) and one flavone (rhamnazin) were screened out as antioxidants. BF in CK contained abundant polyphenolic, which reflected some definite antioxidant properties. The antioxidant compounds consisted at the least of nine PG and one flavone.
研究领域:
药理学
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植物化学
·
中药学
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DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 2
·230
·
IF 4.7
Hua Li,Yurong Mao,Danni Ma,Hua Li,Ruixin Liu,Sirithon Siriamornpun
Water boiling under atmospheric pressure (CAP) and water boiling under high pressure (CHP) are two popular domestic cooking methods for Chinese porridge making. In this study, we aimed to evaluate the effects of these two methods on the phenolic acid composition, antioxidant activity, and starch digestibility of triticale porridges. The contents of total free and total bound phenolic acids in the CHP sample were 1.3 and 1.6 times higher than those in the CAP counterpart, respectively, although the DPPH and ABTS values of these two samples were comparable. CAP induced more small pieces of starch than CHP, and the gelatinization enthalpy was 19% higher in the CHP sample than that in the CAP. Both cooking methods increased the starch digestibility, while the CHP sample (58.84) showed a lower GI than the CAP (61.52). These results may promote the application of triticale in health-promoting staple foods.Keywords:phenolic acid;DPPH radical scavenging ability;ABTS radical scavenging ability;resistant starch;starch digestion;glycemic index;cereal
研究领域:
食品科学
·
营养学
·
化学
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DOI
PubMed
高影响因子
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
·
2012
·Vol. 11
·期 4
·545-551
·
IF 0.82
E Yuan,B Liu,W Li,Q Li
Purpose: To improve the aqueous solubility of phloridzin by complexing it with hydroxypropyl-β-cyclodextrin (HP-β-CD).Methods: The complex of phloridzin with HP-β-CD was prepared by freeze-drying method. The physicochemical properties of the complex were investigated by ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), differential scanning calorimetry (DSC) and x-ray diffractometry (XRD). The antioxidant activity was examined by DPPH and ABTS radical-scavenging activities.Results: Phloridzin in the complex was molecularly dispersed in HP-β-CD matrix. The complex was an effective scavenger of DPPH and ABTS radicals. At a concentration of 0.8 mg/mL and 30 μg/mL, DPPH and ABTS radical scavenging activities of the complex were 83.7 and 74.9 %, respectively.Conclusion: By forming inclusion complex with HP-β-CD, the solubility of phloridzin in water was significantly enhanced. The complex showed strong DPPH and ABTS radical scavenging activities.
研究领域:
药剂学
·
药物化学
·
分子药理学
·
天然产物化学
·
药物递送系统
·
抗氧化研究
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DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 101
·98935-98944
·
IF 3.289
Hongshan Liang,Bin Zhou,Jing Li,Yaqiong Pei,Bin Li
The layer-by-layer (LBL) deposition technique has been widely used to decorate the nanofibers formed from polymer pairs with complementary functional groups. In the current study, an antioxidative coordination-driven multilayer electrospun nanofibrous film was fabricated from tannic acid (TA) and phosvitin (PV) obtained from egg yolk. PV was reported to bond with 95% of yolk iron which could provide chelating sites for TA. The surface morphology of the nanofibrous mats was observed by scanning electron microscopy (SEM). Also the TEM image of the cross-section illustrated a uniform shell formed around the cellulose nanofibers. The deposition of TA and PV was further confirmed by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FT-IR). TA/PV nanofibrous mats showed good 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity regardless of the outmost component. While for superoxide-scavenging and hydroxyl radical-scavenging activity, the outmost component affected the scavenging capacity of nanofibrous mats. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) were combined to characterize the morphology and structure of the deposited mineral phase on the scaffolds after culturing in a simulated body fluid (SBF) for 5 days. From the result, the TA/PV nanofibrous mats were satisfactory for use in bioapplications.
研究领域:
材料科学
·
生物材料
·
组织工程
·
抗氧化研究
·
生物医学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 5
·2701
·
IF 4.9
Shizi Zhang,Yunfeng Xu,Fang Wang,Liyun Yang,Lijuan Luo,Lingyan Jiang
Anthracnose caused byColletotrichum gloeosporioidesis a destructive disease ofStylosanthes(stylo). Combination treatment of phloretin and pterostilbene (PP) has been previously shown to effectively inhibit the conidial germination and mycelial growth ofC. gloeosporioidesin vitro. In this study, the effects of PP treatment on the growth ofC. gloeosporioidesin vivo and the biocontrol mechanisms were investigated. We found that exogenous PP treatment could limit the growth ofC. gloeosporioidesand alleviate the damage of anthracnose in stylo. Comparative transcriptome analysis revealed that 565 genes were up-regulated and 239 genes were down-regulated upon PP treatment during the infection byC. gloeosporioides. The differentially expressed genes were mainly related to oxidative stress and chloroplast organization. Further physiological analysis revealed that application of PP afterC. gloeosporioidesinoculation significantly reduced the accumulation of O2•−level and increased the accumulation of antioxidants (glutathione, ascorbic acid and flavonoids) as well as the enzyme activity of total antioxidant capacity, superoxide dismutase, catalase, glutathione reductase, peroxidase and ascorbate peroxidase. PP also reduced the decline of chlorophyll a + b and increased the content of carotenoid in response toC. gloeosporioidesinfection. These results suggest that PP treatment alleviates anthracnose by improving antioxidant capacity and reducing the damage of chloroplasts, providing insights into the biocontrol mechanisms of PP on the stylo against anthracnose.Keywords:phloretin;pterostilbene;ROS scavenging;chloroplasts;anthracnose;stylo
研究领域:
植物病理学
·
分子生物学
·
植物生理学
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产品关联
DOI
PubMed
高影响因子
Advances in Experimental Medicine and Biology
·
2016
·
IF 1.953
Jin Ju,Li Yan,Zhang Xiuli,Chen Tongsheng,Wang Yifei,Wang Zhiping
Cellular damage such as oxidation and lipid peroxidation, and DNA damage induced by free-radicals like reactive oxygen species, has been implicated in several diseases. Radicals generated by 2,2-azobis (2-amidino-propane) dihydrochloride (AAPH) are similar to physiologically active ones. In this study we found that polydatin, a resveratrol natural precursor derived from many sources, has the capacity of free radical scavenging and antioxidative damage. Using free radical scavenging assays, the IC 50 values of polydatin were 19.25 and 5.29 μg/ml with the DPPH and the ABTS assay, respectively, and 0.125 mg ferrous sulfate/1 mg polydatin with the FRAP assay. With the AAPH-induced oxidative injury cell model assay, polydatin showed a strong protective effect against the human liver tumor HepG2 cell oxidative stress damage. These results indicate that the antioxidant properties of polydatin have great potential for use as an alternative to more toxic synthetic antioxidants as an additive in food, cosmetics and pharmaceutical preparations for the treatment of oxidative diseases. Keywords Free radical scavenging Polydatin Antioxidation AAPH
研究领域:
药理学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
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