PLoS One
·
2014
·Vol. 9
·期 6
·e100731
·
IF 3.534
Ke Xu,Ping Xu
Lactic acid is an important chemical with various industrial applications, and it can be efficiently produced by fermentation, in which Bacillus coagulans strains present excellent performance. Betaine can promote lactic acid fermentation as an effective osmoprotectant. Here, positive effect of betaine on fermentation by B. coagulans is revealed. Betaine could enhance lactic acid production by protecting l -LDH activity and cell growth from osmotic inhibition, especially under high glucose concentrations and with poor organic nitrogen nutrients. The fermentation with 0.05 g/L betaine could produce 17.9% more lactic acid compared to the fermentation without betaine. Beet molasses, which is rich in sucrose and betaine, was utilized in a co-feeding fermentation and raised the productivity by 22%. The efficient lactic acid fermentation by B. coagulans is thus developed by using betaine and beet molasses.
研究领域:
微生物学
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生物工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD ENGINEERING
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2020
·
IF 4.499
Shengjian Xiao,Zihe Li,Qinmei Xiong,Chongde Wu,Jun Huang,Rongqing Zhou,Yao Jin
This work explored the feasibility of sodium lactate as a novel draw solute to conduct the forward osmosis (FO) process in the field of food processing. As a common low-cost food addictive, sodium lactate is non-toxic and shows high osmotic pressure . Based on this knowledge, the results of this work further revealed the combinative effect of osmotic pressure and viscosity of sodium lactate solution on the FO performance. The effect of draw solution concentration, membrane orientation and temperature on the FO performance were also investigated. The results revealed that a maximal average water flux of 56 LMH was reached during the FO experiments with sodium lactate as draw solute. Taking into account the average water flux and the reverse solute flux, 7.1 M was the optimal concentration of the sodium lactate solution for FO process. The application of such draw solution into the concentration of sugar solution and orange juice then validated its promising performance in terms of efficiency, food quality preservation and membrane regeneration.
研究领域:
食品科学
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生物工程
✓ 已验证
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2020
·
IF 7.183
Chang Liu,Yun-Peng An,Jing Yang,Bian-Bian Guo,Hao-Hao Yu,Zhi-Kang Xu
An emerging challenge occurs for recovering ionic liquids (ILs) because of their exponential growth in academic and industrial applications. We report for the first time to reclaim water-soluble ILs from their aqueous solutions by osmotically driving water to spontaneously permeate through semi-permeable membranes. Theoretical simulation indicates a weak hydrability of ILs and a tendency to form micelles in water. We thus demonstrate a significant difference in osmotic pressures between the aqueous solutions of ILs and inorganic salts. Four categories of broadly representative ILs are continuously recovered from their diluent aqueous solutions (~1 wt %) with semi-permeable membranes. Eventually, a record-high concentration up to 58–78 wt % is achieved in this work. Our strategy integrates superiorities of low energy consumption, universal applicability toward ILs, diversified selection of semi-permeable membranes, almost unattenuated water flux and high rejection to ions, hopefully providing new insights into the authentic “green” industrial applications of ILs.
研究领域:
化学工程
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2018
·
IF 13.325
Peng Li,Gong Jinghua,Ma Jinghong
Electro-responsive hydrogels have attracted great attentions because the electric stimuli is easy to be applied and controlled. Thus, it is expected to generate electro-responsive hydrogel fiber to improve electro-response rate. In this paper, graphene oxide/hydrolyzed polyacrylamide/sodium alginate (GO/HPAM/SA) hydrogel fibers were prepared by microfluidic spinning. The structure and morphologies of hydrogel fibers were characterized by FTIR and SEM. The electro-response behaviours were also studied. The results indicate that GO/HPAM/SA hydrogel fibers exhibit bending deformation in an electric field. In Na 2 SO 4 solution, the hydrogel fibers first bend toward the cathode, and then bend toward the anode. It is suggested the bending of the hydrogel fibers is caused by two major factors, one is the osmotic pressure difference, another is the pH gradient induced by electrochemical reactions. Keywords Electro-responsive hydrogel fiber Bending behaviour Osmotic pressure difference pH gradient
研究领域:
材料科学
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生物工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Zhao Cui,Meng Zhang,Yang Yuan,Hui Jia,Huu Hao Ngo,Jie Wang
The focus of this study is on the enhanced concentration effect of the pump effect in forward osmosis (FO) and electroosmotic integrated chip. The study investigates the strengthening effect of the FO process, driven by the pump effect, on the electroosmotic flow process under the same energy consumption, using low-concentration heavy metal ions (Cr 3+ ) as the target enrichment. In the chip system, the pump effect, caused by capillary pressure difference, successfully replaces the osmotic pressure of the draw solution as a new driving force for the FO process. By adjusting the size and number of channels and avoiding errors, the study achieves the highest efficiency of the FO process in the chip, concentrating Cr 3+ from 1.26 × 10 −4 mol/L to 8.01 × 10 −4 mol/L (6.4 times) within 32 min. Excluding the influence of the electrokinetic (EK) limit of low-concentration samples, the concentration efficiency of an individual electroosmotic chip in this study is positively correlated with cross-nanochannel voltage (V cn ). The integrated FO and electroosmosis chip reduce the duration required to concentrate 50 mL Cr 3+ sample to the limit from 90 min to 16 min (5.6 times) compared to the single electroosmotic process. Furthermore, a series of Cr 3+ samples with concentrations below the detection limit of the UV–vis spectrophotometer were concentrated and detected by the integrated chip. The results indicate that the concentration limit of the FO-electroosmotic integrated chip for samples can increase the detection limit of Cr 3+ from 1.37 × 10 −4 mol/L to 3.67 × 10 −6 mol/L.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Bingsen Wang,Haotian Zhang,Nan He,Haonan Wang,Bo Jiang,Dawei Tang,Lin Li
Salt deposition is one of the most significant obstacles to emerging solar interfacial desalination. Several strategies have recently been developed to combat salt deposition. However, the poor performance of most reported salt-resistant evaporators (<1.5 kg m -2 h −1 ) severely restricts the practical applications of this technology. Inspired by seawater-grown mangroves, we propose a bionic hydrogel-aerogel composite (HAC) evaporator with a respiratory root structure in this work. This bionic evaporator consists of a high-osmotic-pressure polyacrylamide (PAM) ionic hydrogel and a hydrophobic aerogel with vapor transport channels. The vapor escape surface is spatially separated from the salt deposition interface by constructing numerous vapor escape channels within the bionic evaporator, resulting in highly efficient salt-resistant evaporation. The HAC evaporator can run salt-free for 50 h in seawater with an evaporation rate of 2.7 kg m -2 h −1 , which is twice that of most salt-resistant evaporators. In addition, the evaporator can achieve evaporation rates of 2.4 kg m -2 h −1 in 20% brine. This design significantly improves the evaporation rate when resistant to salt accumulation, providing a novel strategy for developing highly efficient, long-term stable solar interfacial evaporators.
研究领域:
材料科学
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环境科学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD SCIENCE AND TECHNOLOGY RESEARCH
·
2017
·
IF 0.459
Liangzhi Li,Lina Gu,Xin Ju,Cuiying Hu,Jiaolong Fu,Hongying Cheng,Pei Kang
This study investigated the production of erythritol by Trichosporonoides oedocephalis in response to the varying osmotic pressure. Osmotic pressure was exerted by adding NaCl or KCl into the fermentation broth. It was demonstrated that appropriate raise in osmotic pressure enhanced erythritol production, wherein 5 g/L KCl addition increased erythritol yield by 44.21% compared to the control. The reason for the significant increase in the erythritol production showed that the 5 g/L KCl addition accelerated the glucose utilization in the late period of fermentation time. It was also revealed that 5 g/L KCl increased the activity of erythrose reductase (ER) in the same fermentation period. In bioreactor, the erythritol yield with initial 5 g/L KCl addition was up to 50.54 g/L, which was 47.69% higher than the control (34.22 g/L). The study provided a novel fermentation regulation, that is, economical addition of KCl made more effective production of erythritol when using T. oedocephalis.
研究领域:
微生物学
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发酵工程
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生物技术
✓ 已验证
DOI
高影响因子
Future Microbiology
·
2025
·
IF 2.4
Ao Du,Xiaofen Sun,Meili Dong,Yong Liu,Mingwei Chen,Yuhan Wang,Yuanzhi Zhang,Yao Huang,Zhongsheng Li,Xiang Huang,Yikun Wang,Jingshu Ni
Aims This study aims to investigate how different wound microenvironmental factors (temperature, pH, and osmotic pressure) influence the autofluorescence of Staphylococcus aureus (S. aureus) and its underlying molecular mechanisms, specifically focusing on the porphobilinogen synthase gene (hemB) gene expression.Methods We measured the average fluorescence intensity of S. aureus colonies under varying conditions of pH (3, 5, 7, 9, 11), temperature (25°C, 31°C, 37°C, 43°C), and osmotic pressure (0.9%, 1.8%, 2.7%, 3.6%) over time. Fluorescence intensity was quantified using ImageJ software. Additionally, RT-qPCR was used to analyze the expression levels of the hemB under these conditions.Results Bacterial fluorescence intensity increased as the temperature ranged from 25°C to 43°C, with corresponding upregulation of hemB expression. At pH values between 3 and 11, fluorescence intensity decreased as pH increased, reflecting a similar trend in hemB expression. Fluorescence also diminished with higher osmotic pressures (0.9% to 3.6%), mirroring the downregulation of hemB.Conclusions Our findings indicate that temperature, pH, and osmotic pressure significantly affect the autofluorescence of S. aureus by modulating porphyrin accumulation through hemB gene expression. These environmental factors should be considered when using bacterial fluorescence for wound infection assessment.
研究领域:
微生物学
·
分子生物学
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医学
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产品关联
DOI
PubMed
高影响因子
Small
·
2018
·Vol. 14
·期 39
·1802107
·
IF 9.598
Youchuang Chao,Sze Yi Mak,Shakurur Rahman,Shipei Zhu,Ho Cheung Shum
Droplets containing ternary mixtures can spontaneously phase-separate into high-order structures upon a change in composition, which provides an alternative strategy to form multiphase droplets. However, existing strategies always involve nonaqueous solvents that limit the potential applications of the resulting multiple droplets, such as encapsulation of biomolecules. Here, a robust approach to achieve high-order emulsion drops with an all-aqueous nature from two aqueous phases by osmosis-induced phase separation on a microfluidic platform is presented. This technique is enabled by the existence of an interface of the two aqueous phases and phase separation caused by an osmolality difference between the two phases. The complexity of emulsion drops induced by phase separation could be controlled by varying the initial concentration of solutes and is systematically illustrated in a state diagram. In particular, this technique is utilized to successfully achieve high-order all-aqueous droplets in a different aqueous two-phase system. The proposed method is simple since it only requires two initial aqueous solutions for generating multilayered, organic-solvent-free all-aqueous emulsion drops, and thus these multiphase emulsion drops can be further tailored to serve as highly biocompatible material templates.
研究领域:
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Zheming Gu,Xin Tang,Bingyong Mao,Qiuxiang Zhang,Jianxin Zhao,Wei Chen,Shumao Cui
Bifidobacterium bifidum rarely utilises yeast extract , which causes significant challenges in its industrial production. This study characterised the utilisation of amino acids by B. bifidum to improve the viability of the strain. In this study, the proliferation effect of B. bifidum in different nitrogen sources was determined, and the amino acid contents of different nitrogen sources were analysed, which indicated that hydrophobic amino acids may inhibit B. bifidum growth. Among different hydrophobic amino acids , methionine was identified as the strongest growth inhibitor. Furthermore, the intracellular pH and NH 4 + contents of B. bifidum CCFM16 were higher in methionine-free medium. Among the NH 3 -producing enzymes related to amino acid metabolism , the metC gene was significantly transcribed in methionine-free medium. In addition, tcyP , metB , metC and metE genes encoding cysteine transporter proteins and the conversion pathway of cysteine to methionine were transcribed 2.03-fold, 7.03-fold, 3.05-fold and 2.92-fold, respectively, in methionine-free medium compared to methionine-containing medium. Compared with methionine-containing medium, the activity of tricarboxylic acid cycle rate-limited enzyme isocitrate dehydrogenase in methionine-free medium also increased from 198.89 to 410.56 nmol/min/mg protein, which probably enhanced the carbon flux and promoted growth. Meanwhile, the activity of pyrroline-5-carboxylate reductase , an enzyme that synthesises proline , increased from 14.76 to 35.28 nmol/min/mg protein, which promoted proline synthesis in response to osmotic stress . Cyclopropane fatty acids were also significantly higher in methionine-free medium, which may promote growth by decreasing cell membrane permeability to protons and enhancing cell membrane fluidity . This study provides a theoretical basis for high-density culture of B. bifidum .
研究领域:
微生物学
·
生物化学
✓ 已验证
DOI
高影响因子
Aquaculture Reports
·
2023
·
IF 3.7
Xinyu Song,Wengang Lü,Salifu Ibrahim,Yuewen Deng,Qi Li,Chenyang Yue
Crassostrea hongkongensis (Hong Kong oyster), a commercially valuable bivalve thriving in the estuaries along the northern coast of the South China Sea, is threatened by the increase of salinity during the dry season. It is essential to improve its hyper-salinity tolerance, which requires a clear understanding of the mechanism to regulate its osmotic balance. For this purpose, 25 free amino acids (FAA), the most critical intracellular osmolyte in bivalves, were quantified in gill, adductor muscle, and hemolymph of C. hongkongensis during abrupt salinity changes (18–6, 12, 24 and 30‰) by high-performance liquid chromatography-tandem mass spectrometry. Taurine, alanine and glycine were dominant FAA, contributing to total FAA contents that varied with salinities, no matter in gill, muscle or hemolymph. Under hypo-osmotic stress, total FAA contents decreased in gill and adductor muscle but increased in hemolymph, probably resulting from the extrusion of some FAA from tissues to prevent swelling of cells. And taurine contributed most significantly to the hypo-salinity response, which was mainly reflected by the remarkable increase in hemolymph at 8 h after the exposure to 6‰. By contrast, total FAA contents increased in gill, muscle, and hemolymph when transferred to hyper-salinity, suggesting the assumption that it increases the osmolality and subsequently prevents the cell shrinking. Alanine and glycine contributed most significantly to hyper-salinity response. Of the two, the former was more sensitive to hyper-osmotic stress for its rapid increase in gill within 8 h after the exposure to 24 and 30‰. An inverse pattern with most FAA was presented by ornithine, the intermediate of the urea cycle, which was probably associated with the FAA metabolism. This study identified the major FAA as intracellular osmolytes in C. hongkongensis and would benefit the further understanding of the regulatory mechanisms of oyster salinity acclimation.
研究领域:
海洋生物学
·
分子生物学
✓ 已验证
DOI
高影响因子
ANIMAL FEED SCIENCE AND TECHNOLOGY
·
2022
·
IF 3.313
Shiwei Xie,Dan Wei,Yongjian Liu,Lixia Tian,Jin Niu
Dietary fish meal (FM) and fish oil (FO) replacement are crucial for the sustainable development of aquaculture. An eight weeks feeding trial was conducted to evaluate the potential replacing FM with soy protein concentrate , and the suitable FO level for a low FM diet of Penaeus monodon . A diet containing 25% FM was used as the control (C diet), and three other diets were formulated to contain 15% FM, and supplemented with 3%, 2%, 1% FO [high FO (HF), medium FO (MF) and low FO (LF), respectively]. The experiment was conducted in quadruplicates (30 shrimp per replicate, average weight 1.00 ± 0.01 g). The results indicated that besides the final body weight, there were no differences in weight gain, feed efficiency and survival rate observed among the four groups. The results of the acute salinity stress showed that shrimp fed the C and MF diets showed a similar survival rate, which was significantly higher than those fed a LF diet. Shrimp that were fed HF, MF and LF diets showed a lower level of fatty acid transport-related mRNA expression in the hepatopancreas , compared with those fed the C diet. Shrimp fed HF, MF and LF diets showed lower expression levels of superoxide dismutase , endoplasmic reticulum protein 57 and immune deficiency (IMD) in the hepatopancreas than those fed the C diet. Shrimp that were fed a LF diet showed the lowest expression levels of inhibitor of apoptosis proteins, Toll and tumor necrosis factor receptor associated factor 6 (TRAF6) in the hepatopancreas, as well as the highest expression levels of Toll, extracellular signal-regulated kinase, Relish and TRAF6 in the intestine among four groups. Intestinal microstructure damage was observed in shrimp that were fed a LF diet, and intestinal endoplasmic reticulum (ER) swelling was observed in shrimp fed MF and LF diets. In conclusion, these results indicated that the growth performance of shrimp was not affected when the FM and FO contents decreased, whereas a low content of FM negatively influenced the immune response in shrimp by modulating IMD and ER stress related gene expression. Under low FM conditions, a suitable dietary FO level would benefit to the lipid transport, hepatopancreas and intestine health, as well as the immune response of shrimp.
研究领域:
水产养殖
·
营养学
·
分子生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）