FOOD CHEMISTRY
·
2019
·
IF 5.399
Ye Yuan,Xuexi Wang,Min Jin,Lefei Jiao,Peng Sun,Mónica B. Betancor,Douglas R. Tocher,Qicun Zhou
Lipid sources as alternatives to fish oil could alter the nutritional value and flavor quality of crab meat affecting consumer preferences. Herein, an 8-week nutritional trial was designed to investigate the effects of dietary lipid sources including fish oil (FO), krill oil (KO), palm oil, rapeseed oil, soybean oil and linseed oil on profiles of amino acids, fatty acids and volatiles in muscle of swimming crab ( Portunus trituberculatus ). Volatiles of crab muscle were characterized by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry. Results revealed that crabs fed FO and KO had significantly higher levels of protein, indispensable amino acids, eicosapentaenoic acid and docosahexaenoic acid in muscle. Principal component analysis and hierarchical cluster analysis demonstrated that muscle volatiles of crabs fed different dietary oils exhibited significant variations. Dietary FO and KO significantly increased the relative levels of 3-methylbutanal, heptanal, benzaldehyde and nonanal in muscle, which may produce more pleasant flavors.
研究领域:
水产养殖
·
营养学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxics
·
2025
·Vol. 13
·期 5
·395
·
IF 4.1
Feihu Qin,Nanjing Zhao,Gaofang Yin,Yunfei Luo,Tingting Gan
The agricultural production process contributes to the global issue of pesticide pollution. Based on the static toxicity test of diuron (DCMU) onDaphnia magna(D. magna) for EC50-48 h, a concentration range of 0.2 to 1 mg/L was set as sublethal concentrations, while lethal concentrations were set at 2 mg/L and 4 mg/L. This study analyzes the toxic response patterns of the swimming behavior indicators ofD. magnaexposed to different concentrations of DCMU. The results showed that the average speed (V) ofD. magnadecreased step by step with exposure time, regardless of exposure to sublethal concentration or lethal concentration. However, during the same short-term exposure period, the V ofD. magnaat lethal concentration was higher than that at sublethal concentration, which indicates that the swimming behavior ofD. magnaexposed to DCMU may be stimulated and accelerated. Compared to the control group, there is a statistically significant difference in the V ofD. magnaafter short-term exposure, especially showing an extremely significant difference after 5 min of exposure. Evidently, compared to the traditional 48 h static toxicity testing method, the swimming behavior indicators ofD. magnashow a more sensitive response to DCMU after 5 min of exposure, making it more suitable for rapid toxicity detection. By expanding the range of exposure concentrations, it was found that the V indicator ofD. magnaresponded significantly to a DCMU concentration of 0.05 mg/L after only 5 min of exposure, and a high degree of correlation was observed between the indicator and the exposure concentration. Through nonlinear fitting, the relationship between V and the dose–effect of DCMU toxicity presents an S-shaped curve, with R2> 0.9. Consequently, it becomes possible to study the dose–effect relationship between the changes in the swimming behavior indicators ofD. magnaand the stress concentration based on this theory. This further establishes a foundation for the development of comprehensive aquatic toxicity rapid detection technology based on the toxic response of swimming behavior indicators.
研究领域:
环境科学
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毒理学
·
水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 7
·2841-2854
·
IF 4.813
Zhou Gang,Wang Ying-si,Peng Hong,Li Su-juan,Sun Ting-li,Shen Peng-fei,Xie Xiao-bao,Shi Qing-shan
Abstract The genus Citrobacter is commonly found in environmental and industrial settings, some members of which have been used for bioremediation of heavy metals owing to the absorption ability of their biofilms. Although our previous studies have found that the outer membrane protein A (OmpA) contributes to the process of Citrobacter werkmanii biofilm formation, the underlying mechanisms remain elusive. Therefore, we deleted ompA from the genome of C. werkmanii and investigated its phenotypes in comparison to the wild type strain (WT) and the complementary strain using biochemical and molecular techniques including RNA-Seq. Our results demonstrated that the deletion of ompA led to an increase in biofilm formation on both polystyrene and glass surfaces due to upregulation of some biofilm formation related genes. Meanwhile, swimming ability, which is mediated by activation of flagellar assembly genes, was increased on semi-solid plates in the ∆ompA strain when compared with WT. Additionally, inactivation of ompA also caused increased 1,2-benzisothiazolin-3-one (BIT) resistance, differential responses to Ca 2+ stress, curli protein expression and cellulose production. Finally, ∆ompA caused differential expression of a total of 1470 genes when compared with WT, of which 146 were upregulated and 1324 were downregulated. These genes were classified into different Gene Ontology (GO) and KEGG pathways. In summary, ompA in C. werkmanii contributes to a variety of biological functions and may act as a target site to modulate biofilm formation. Key points
• ompA is a negative regulator for biofilm formation by C. werkmanii.
• ompA inhibits swimming motility of C. werkmanii.
• ompA deletion causes different expression profiles in C. werkmanii.
研究领域:
微生物学
·
分子生物学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Chuan Wei Zhang,Weifeng Zou,Hai Chao Yu,Xing Peng Hao,Guorui Li,Tiefeng Li,Wei Yang,Zi Liang Wu,Qiang Zheng
The design of soft robots capable of navigation underwater has received tremendous research interest due to the robots’ versatile applications in marine explorations. Inspired by marine animals such as jellyfish, scientists have developed various soft robotic fishes by using elastomers as the major material. However, elastomers have a hydrophobic network without embedded water, which is different from the gel-state body of the prototypes and results in high contrast to the surrounding environment and thus poor acoustic stealth. Here, we demonstrate a manta ray-inspired soft robot fish with tailored swimming motions by using tough and stiff hydrogels as the structural elements, as well as a dielectric elastomer as the actuating unit. The switching between actuated and relaxed states of this unit under wired power leads to the flapping of the pectoral fins and swimming of the gel fish. This robot fish has good stability and swims with a fast speed (∼10 cm/s) in freshwater and seawater over a wide temperature range (4–50 °C). The high water content (i.e., ∼70 wt %) of the robot fish affords good optical and acoustic stealth properties under water. The excellent mechanical properties of the gels also enable easy integration of other functional units/systems with the robot fish. As proof-of-concept examples, a temperature sensing system and a soft gripper are assembled, allowing the robot fish to monitor the local temperature, raise warning signals by lighting, and grab and transport an object on demand. Such a robot fish should find applications in environmental detection and execution tasks under water. This work should also be informative for the design of other soft actuators and robots with tough hydrogels as the building blocks.
研究领域:
仿生学
·
软体机器人
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 47
·28267-28276
·
IF 3.119
Huihui Zhao,Linyan Yang,Yejin Li,Weibo Xue,Kai Li,Yingqi Xie,Shujuan Meng,Guomin Cao
A prevalent group of disinfection by-products (DBPs), i.e., haloacetic acids (HAAs), have raised significant public concern due to their high frequency of occurrence, considerable concentrations and potent toxicity. This study investigated the environmental occurrence of HAAs and the corresponding predicted human exposure to two important water matrices where humans are frequently and long-term exposed, i.e., swimming pool waters (SPWs) and drinking waters (DWs), in Shanghai, China. The sum of five HAAs in SPWs was 241 μg L−1 on average (dominated by dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA)), four times as much as its maximum contaminant level (MCL) regulated by the Environmental Protection Agency (EPA) in DWs. The maximum HAA concentration in DWs was 35 μg L−1, with chloroacetic acid (CAA) as the most dominant compound. The higher concentrations (reflected by total organic carbon (TOC), dissolved organic carbon (DOC), and UV254) and more categories (body fluids and personal care products in addition to natural organic matter) of organic contaminants and the higher chlorine in SPWs should be responsible for the differentiated HAA yield and speciation. The qualification rates of samples have been evaluated. The power function models correlating HAAs with multiple water quality parameters were developed with correlation coefficients of 0.614 and 0.798 for SPWs and DWs respectively. These models may provide the preliminary insights on how to minimize HAA formation by grasping the relative importance of each parameter and how to build the framework to predict HAA formation in untreated source water or SPWs subjected to chlorination. The predicted carcinogenic and non-carcinogenic risks of humans exposed to SPWs and DWs with HAAs were within the acceptable levels.
研究领域:
环境科学
·
公共卫生
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Metabolites
·
2025
·Vol. 15
·期 2
·134
·
IF 3.7
Yunchen Meng,Yiling Hu,Yaqi Xue,Zhiping Zhen
Objectives:This study aimed to investigate the regulatory impact of early swimming intervention on striatal metabolism inShank3gene knockout ASD model rats.Methods:Shank3gene knockout exon 11–21 male 8-day-old SD rats were used as experimental subjects and randomly divided into the following three groups: aShank3knockout control group (KC), a wild-type control group (WC) from the same litter, and aShank3knockout swimming group (KS). The rats in the exercise group received early swimming intervention for 8 weeks starting at 8 days old. LC-MS metabolism was employed to detect the changes in metabolites in the striatum.Results:There were 17 differential metabolites (14 down-regulated) between the KC and WC groups, 19 differential metabolites (18 up-regulated) between the KS and KC groups, and 22 differential metabolites (18 up-regulated) between the KS and WC groups.Conclusions:The metabolism of striatum inShank3knockout ASD model rats is disrupted, involving metabolites related to synaptic morphology, and the Glu and GABAergic synapses are abnormal. Early swimming intervention regulated the striatal metabolome group of the ASD model rats, with differential metabolites primarily related to nerve development, synaptic membrane structure, and synaptic signal transduction.
研究领域:
神经科学
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代谢组学
·
行为学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Current Research in Food Science
·
2022
·
IF 6.269
Yong Chen,Jiajun Wang,Ziheng Jing,Jose M. Ordovas,Jing Wang,Lirong Shen
Demands for dietary supplements with anti-fatigue effects are growing fast due to increasing societal demands. Moreover, in highly physically active individuals, there are also significant needs for supplements to improve exercise performance. The present study evaluated the potential anti-fatigue and anti-oxidant effects of curcumin in mice using exhaustive swimming test. Male C57BL/6J mice were randomized into six groups: blank control (Rest), swimming control (Con), Vitamin C (Vc), low-dose curcumin (C 50 ), middle-dose curcumin (C 100 ), and high-dose curcumin (C 200 ). After a 4-week intervention, the mice in all groups except the Rest group were subject to an exhaustive swimming test. Then, mice were sacrificed to examine serum biochemical markers and fatigue-related enzymes. Moreover, the gene and protein expressions of signal transduction factors involved in the Nrf2/Keap1 signaling pathway were measured. The results indicated that curcumin significantly enhanced the exercise tolerance of mice in the exhaustive swimming test. Particularly, the swimming time of mice in the C 100 group was increased by 273.5% when compared to that of mice in the Con group. The levels of blood urea nitrogen, blood ammonia, lactic acid, creatine kinase and lactate dehydrogenase in the C 100 group were decreased by 13.3%, 21.0%, 18.6%, 16.7% and 21.9%, respectively, when compared to those of mice in the Con group. Curcumin alleviated exercise-induced oxidative stress and significantly enhanced the activities of superoxide dismutase, catalase and glutathione peroxidase by activating the Nrf2 signaling. These findings indicated that curcumin supplementation exerted remarkable anti-oxidant and anti-fatigue effects in mice, providing additional evidence supporting the use of curcumin as functional food, especially by those engaged in sports-related activities.
研究领域:
营养学
·
运动生理学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2023
·
IF 3.9
Yixi Tao,Chunying Du,Bicheng Duan,Wenbo Wang,Hui Guo,Jingyun Feng,Hao Xu,Yun Li
Eugenol is a natural phenolic essential oil extracted from cloves, that has analgesic and anesthetic effects and is widely used in fishery anesthesia. However, the potential safety risks of aquaculture production associated with the massive use of eugenol and its developmental toxicity during early life stages of fish have been overlooked. In this study, zebrafish ( Danio rerio ) embryos at 24 hours post-fertilization (hpf) were exposed to eugenol at concentrations of 0, 10, 15, 20, 25, or 30 mg/L for 96 h. Eugenol exposure delayed the hatching of zebrafish embryos, and reduced the body length and the inflation rate of the swim bladder. The accumulated number of dead zebrafish larvae in the eugenol-exposed groups was higher than that of the control group, and it was dose-dependent. Real-time quantitative polymerase chain reaction (qPCR) analysis showed that the Wnt/β-catenin signaling pathway that regulates the development of the swim bladder during the hatching and mouth-opening stages was inhibited after eugenol exposure. Specifically, the expression of wif1 , a Wnt signaling pathway inhibitor, was significantly up-regulated, whereas the expression of fzd3b , fzd6 , ctnnb1 , and lef1 involved in the Wnt/β-catenin pathway was significantly down-regulated. These results suggest that the failure of zebrafish larvae to inflate their swim bladders as a result of eugenol exposure may be caused by the inhibition of the Wnt/β-catenin signaling pathway inhibited. In addition, the inability to catch food due to the abnormal development of the swim bladder may be the key to the death of zebrafish larvae during the mouth-opening stage.
研究领域:
水产养殖学
·
发育生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2019
·
IF 7.913
Liangchen Long,Yinan Bu,Baiyang Chen,Rehan Sadiq
To discover an applicable technology for urea abatement from swimming pool water (SPW), this study compared the performances of seven ultraviolet (UV)-based technologies on urea removal, including UV alone, UV coupled with hydrogen peroxide (UV/H 2 O 2 ), sulfite (UV/Na 2 SO 3 ), potassium persulfate (UV/K 2 S 2 O 8 ), a combination of UV and vacuum UV (UV/VUV), and UV/VUV in tandem with either H 2 O 2 (VUV/H 2 O 2 ) or potassium persulfate (VUV/K 2 S 2 O 8 ). Among them, UV and UV/Na 2 SO 3 showed little removal ability, and UV/H 2 O 2 removed only 12.8% of urea within 3-h experiments, while UV/VUV degraded 71.7% of urea without introducing substantial total dissolved solids (TDS). Therefore, UV/VUV was considered as a promising technology for further exploration. In comparison, although UV/K 2 S 2 O 8 exhibited higher urea removal than UV/VUV, it caused dramatic increases of TDS, which made the regulatory threshold for the TDS increment difficult to maintain. Within UV/VUV studies, some common components in SPW (e.g., cyanuric acid , humic acid , nitrate, and bicarbonate) inhibited the removal process, whereas chloride and sulfate facilitated it, while free chlorine at doses ≤ 3 mg-Cl 2 /L and pH levels from 6.8 to 8.0 imposed little impact on urea degradation. Overall, UV/VUV degraded 40.0% and 22.2% of urea from tap water and SPW, respectively; both were lower than the efficiency observed in ultrapure water. As for reaction byproducts, urea phototransformation via UV/VUV yielded nitrate and ammonia as the key products with the mass balance of nitrogen element being met. However, the contents of organic carbon decreased at a rate slightly lower than urea degradation, suggesting that urea was mostly mineralized and slightly converted to unknown organic compounds. The results hence demonstrate that UV/VUV is an effective alternative for urea removal from SPW.
研究领域:
环境科学
·
水处理工程
✓ 已验证
DOI
高影响因子
Environmental Science-Water Research & Technology
·
2017
·Vol. 4
·期 2
·218-225
·
IF 2.817
Fang Yang,Zhaoguang Yang,Haipu Li,Fangfang Jia,Ying Yang
Swimming pool disinfection by-products (DBPs) have aroused widespread concern in many countries due to their adverse human health effects. In this study, the concentration levels of several kinds of DBPs including trihalomethanes, haloacetonitriles and trichloronitromethane were determined in 35 outdoor swimming pools in Hunan, China. The main water parameters investigated in this study included free residual chlorine, total organic carbon, pH, urea, UV254, total nitrogen and temperature. The management and operation of pools were comprehensively investigated as well. The results showed that the mean concentrations of trihalomethanes, haloacetonitriles and trichloronitromethane in the swimming pools were 21 ± 14 μg L−1, 2.1 ± 1.4 μg L−1 and 0.91 ± 0.51 μg L−1, respectively. Chloroform was the most predominant DBP species detected, and dichloroacetonitrile was the most predominant nitrogenous DBP (N-DBP) species detected. Free residual chlorine and pH might be critical factors in controlling the occurrence of DBPs. Showering before entering the pool could reduce the organic matter from swimmers, and this practice seemed to be an effective way to reduce the formation of DBPs. It is worth noting that urea, as one of the most predominant organic nitrogen compounds in human sweat and urine, had a high concentration in the investigated swimming pool samples. The cancer risks of DBPs in these outdoor swimming pools were lower than the US EPA guidelines in general, while the harm of DBPs couldn't be overlooked at high concentrations.
研究领域:
环境科学
·
公共卫生
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Sciences
·
2022
·
IF 6.796
Di Zhang,Shengkun Dong,Li Chen,Rong Xiao,Wenhai Chu
Quantification of regulated and emerging disinfection byproducts (DBPs) in swimming pool water, as well as the assessment of their lifetime health risk are limited in China. In this study, the occurrence of regulated DBPs (e.g., trihalomethanes, haloacetic acids) and emerging DBPs (e.g., haloacetonitriles, haloacetaldehydes) in indoor swimming pool water and the corresponding source water at a city in Eastern China were determined. The concentrations of DBPs in swimming pool water were 1-2 orders of magnitude higher than that in source water. Lifetime cancer and non-cancer risks of DBPs stemming from swimming pool water were also estimated. Inhalation and dermal exposure were the most significant exposure routes related to swimming pool DBP cancer and non-cancer risks. For the first time, buccal and aural exposure were considered, and were proven to be important routes of DBP exposure (accounting for 17.9%-38.9% of total risk). The cancer risks of DBPs for all swimmers were higher than 10 −6 of lifetime exposure risk recommended by United States Environmental Protection Agency, and the competitive adult swimmers experienced the highest cancer risk (7.82 × 10 −5 ). These findings provide important information and perspectives for future efforts to lower the health risks associated with exposure to DBPs in swimming pool water.
研究领域:
环境科学
·
公共卫生
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2022
·Vol. 61
·期 4
·339-346
·
IF 1.555
Zhou Jinsen,Liu Cimin,Chen Yong,Luo Xiaoyan,Deng Dongsheng
A reversed-phase isocratic elution high-performance liquid chromatography method coupled with fluorescence detection has been developed to determine urea concentration via online postcolumn derivatization. Swimming pool water samples were filtered through 0.20 μm syringe filters. When the temperature of reaction coil was 40°C, urea was derivatized well with xanthydrol methanol solution (0.1 g/L) containing 0.50% hydrochloric acid with a flow rate of 0.20 mL/min. Successful separation was achieved by using Shim-pack VP-ODS C18 (250 mm × 4.6 mm, 5 μm) column, with a mobile phase containing phosphoric acid solution (0.01 mol/L) at a flow rate of 0.80 mL/min. Retention time and external standard method were used for qualitative and quantitative urea analysis, respectively. Under the established conditions, the limit of detection, linear range, correlation coefficient, recovery and relative standard deviation was 0.09 mg/L, 1.0–100.0 mg/L, 0.9998, 87.0–105.3% and 0.95–4.8%, respectively. Ammonia, thiourea and trichloroisocyanuric acid did not interfere with urea analysis. The method showed satisfactory results with high precision, accuracy, recovery, as well as sensitivity, for the determination of urea in swimming pool water.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）