ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Yijing Han,Qianqian Zhang,Lizhu Chen,Jianmin Zhao,Dinglong Yang
Deltamethrin (DLM), a broad-spectrum pesticide, has been proven to have toxic effects on aquatic organisms. Here, we detected the formation of extracellular traps (ETosis) formation in Manila clam ( Ruditapes philippinarum ) hemocytes stimulated by three concentrations of DLM (0.01, 0.1 and 1 μg/mL) in vitro, and explored the underlying mechanisms induced by this pesticide. Extracellular DNA structure observation and quantitative results indicated that DLM exposure could obviously induce hemocytes ETosis, especially under high concentration of DLM induction. Moreover, DLM increased the levels of myeloperoxidase (MPO) and reactive oxygen species (ROS) in a dose-dependent manner, and enhanced the mRNA expression of several ROS-related genes. DPI (NADPH oxidase inhibitor) and ABAH (MPO inhibitor) could substantially inhibit DLM-induced extracellular traps (ETs), suggesting that the induced ETs release was caused by the induction of the ROS burst and MPO production. In addition, three concentrations of DLM-induced ETs were also accompanied by mitochondrial dysfunction, such as increasing the production of mitochondrial ROS, leading to a decrease in mitochondrial membrane potential (MMP) and activation of mitochondrial permeability transition pore (MPTP). Taken together, these results will shed new light on the immunotoxicity of DLM in clams and perhaps lays the foundation for health assessment in bivalves.
研究领域:
水生毒理学
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免疫毒理学
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海洋生物学
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环境科学
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高影响因子
AQUATIC TOXICOLOGY
·
2025
·
IF 4.3
Hao Wu,Jinwei Gao,Dong Liu,Xiangrong Liu,Feiming Chen,Jiayuan Qin,Yunfei Ye,Zhenzhen Xiong,Jiayu Wu,Can Yang,Min Xie,Rui Song,Dongsheng Ou
In recent years, deltamethrin (DEL) pollution poses a significant threat to aquaculture; however, the protective role of vitamin C (VC) against acute toxicity in crucian carp has not been well understood. This study provides compelling evidence that exposure to 2.0 mg/L waterborne VC significantly mitigates DEL-induced 96-hour mortality in crucian carp ( p < 0.001). Importantly, this research represents the first documentation of the protective effects of waterborne VC in fish, which is mediated through the modulation of the aryl hydrocarbon receptor (AhR)/nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway—a mechanism not previously reported in crucian carp. The observed protective effect is attributed to the enhancement of antioxidant and anti-inflammatory responses via this signaling pathway. Exposure to DEL at 6.2 μg/L resulted in severe oxidative stress, characterized by significantly increased malondialdehyde (MDA) levels and depletion of catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD) in the liver and intestine ( p < 0.01). Histopathological damage, including hepatic vacuolation, shortened villi, and inflammation, as well as elevated reactive oxygen species (ROS), was observed. Co-exposure to VC normalized antioxidant markers, reduced MDA and ROS levels, and alleviated tissue damage ( p < 0.05). Mechanistically, VC counteracted the suppression of AhR/Nrf2 by DEL, leading to the statistically significant upregulation of antioxidant genes ( gst, sod, cat ) and suppression of pro-inflammatory cytokines ( il1β, il6, tnfα ) ( p < 0.05). VC exhibited tissue-specific regulation by enhancing hepatic Nrf2 defenses and co-activating intestinal AhR/Nrf2, as confirmed by Western blot analysis. Our study shows that waterborne VC effectively protects against acute DEL toxicity under current experimental conditions, suggesting its potential as an eco-friendly emergency intervention in aquaculture during sudden pesticide exposure. Further research is needed to confirm its effectiveness in complex pond systems and various fish species.
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高影响因子
3 Biotech
·
2025
·Vol. 15
·期 12
·412
·
IF 2.9
Fang Zhiruo,Zhu Haojun,Gao Jiancao,Cao Liping,Yao Yu,Li Quanjie,Dai Fei,Yang Xiwei,Du Jinliang,Xu Gangchun
Silymarin has proven antioxidant activity and hepatoprotective effects, but no study to date has investigated the potential protective effect on deltamethrin-induced hepatopancreas injury in Chinese mitten crabs Eriocheir sinensis . This study investigated the protective effect of silymarin pretreatment on deltamethrin-induced hepatopancreas injury in E.sinensis . Healthy crabs (n = 150) were randomly divided into five experimental groups: the control, a deltamethrin exposure group (Basal feed without silymarin), and three silymarin groups (addition of 0.1, 0.5, or 1.0 g/kg diet to the basal feed). After 6 weeks of feeding (no deltamethrin exposure), the crabs were exposed to a deltamethrin concentration of 4.317 μg/L for 48 h. Histopathological results showed that the hepatopancreas remained relatively intact in the silymarin pretreatment groups. Furthermore, metabolites annotated by LC–MS were mainly enriched in signaling pathways related to cytochrome P450, terpenoid alkaloid biosynthesis and phospholipid metabolism. Silymarin at 0.1, 1.0 and/or 0.5 g/kg were significantly increased AKP, ACP, GSH activities and T-AOC levels, and significantly decreased AST, ALT, TNF-α, IL-6, NO, TC, MDA, CYP3A and CYP2E1 levels ( P < 0.05). Silymarin at the concentation of 0.1, 0.5 and 1.0 g/kg could significantly up-regulated the expressions of immune-related genes ( propo , alf3 , crustin1 , hsp70 , hsp90 , relish ), anti-apoptotic gene ( bcl-2 ), and significantly down-regulated the lipid metabolism-related genes ( cpt1 , srebp , acc ) and apoptosis-related genes ( caspase3 , caspase8 , p53 , cyt-c ) ( P < 0.05). Silymarin at 0.5 and/or 1.0 g/kg significantly down-regulated the mRNA expression of inflammation-related genes ( tnf-α , il-16 ) and drug metabolism-related genes ( cyp2a , cyp2e1 , cyp3a ) ( P < 0.05). The results demonstrate a good protective effect of 1.0 g/kg silymarin on deltamethrin-induced hepatopancreas injury induced by deltamethrin in a crustacean species.
研究领域:
水产毒理学
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环境科学
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分子生物学
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渔业科学
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药理学
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Lin-Lin Zhang,Zeng Chen,Li Li,Wuying Yang,Dan Wang,Yanping Hong,Kaijie Tang,Jiaxin Hong,Lu-Ying Duan,Limin Lu
Developing a multifunctional sensor that can detect food-related multi-analytes is significant for food safety but remains challenging due to the difficulty of integrating multiple functions into one sensor. Herein, a dual-luminescent europium metal-organic framework (EuMOF) was elaborately synthesized through a simple self-assembly process, during which Eu 3+ coordinated with 2-hydroxyterephthalic acid (HTPA) and 1,10-phenanthroline that delivered strong characteristic fluorescence of HTPA and Eu 3+ . The resultant EuMOF exhibited good detection performance toward Sn 4+ and amitraz with different response behaviors. As for target Sn 4+ , the remarkably enhanced fluorescence from HTPA was observed and the fluorescence for Eu 3+ decreased significantly, which was attributed to the structural disintegration of EuMOF caused by Sn 4+ . Whereas, the presence of amitraz weakened both luminescence of HTPA and Eu 3+ owing to the synergistic function of inner filter effect and photoinduced electron transfer. Furthermore, an intelligent and miniature sensor that incorporated EuMOF-based test paper with color recognition application was exploited for portable visual analyzing Sn 4+ and amitraz in real samples, affording great possibilities for on-site monitoring of food hazards.
研究领域:
材料科学
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分析化学
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食品安全
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传感器与生物传感器
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环境监测
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APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Guangling He,Shuqing Wei,Yuhao Yue,Lei Dong,Liangmin Yu
Hydrogel is recognized as highly promising antifouling coatings. The introduction of other antifouling strategies has emerged as an essential research direction for enhancing hydrogel antifouling applications. However, simultaneous integration of multiple antifouling strategies within the same hydrogel remains challenging, while attenuation of hydrogel hydration layer antifouling effect during functionalization is still problematic. Herein, a multifunctional coating combines the hydrogel hydration layer surface with multiple antifouling mechanisms including low surface energy characteristics, cationic contact-killing structure, and fluorescence performance was developed. Through elevation of 2-(dimethylamino)ethyl methacrylate (DM) content, the hydration layer characteristics, low surface energy characteristics, cationic contact-killing effect, and fluorescence performance were simultaneously enhanced. This approach effectively overcomes antagonistic interactions between co-existing multiple antifouling mechanisms. Crucially, the fluorescence functionality was engineered in this coating without employing exogenous fluorophores through precisely designed intermolecular aggregation. Meanwhile, an enhanced dual-stage defense was imparted to the coatings through synergistic mechanism integration. First stage of antifouling defense: hydration layer, low surface energy, and fluorescence performance collectively impart enhanced fouling-resistance adhesion capability into coating. Second stage of antifouling defense: surface-adhered organisms are killed via contact-killing. Consequently, the hydrogel demonstrates excellent anti-protein adsorption performance (minimum normalized value <1 %), algal adhesion and growth inhibition performance, inhibition of bacterial growth (up to 98.27 %) and adhesion performance (up to 96.36 %). It employs absolutely no metallic antifouling agents and fluorescent agents, thereby providing important insights for eco-friendly marine antifouling coatings.
研究领域:
材料科学
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高分子化学
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表面工程
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海洋生物污损防治
·
环境友好材料
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功能涂层
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生物医学工程
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Yeru Liu,Mengxue Zhang,Xiaolei Zhao,Yifan Zhou,Hongna Yin,Li Zhou,Zhicheng Liu,Qiyong Cai
Glyphosate, a broad-spectrum herbicide extensively applied in agriculture, may bioaccumulate in humans via the food chain and pose toxicological risks. In this work, an integrated biosensing strategy for glyphosate is developed, integrating a self-developed Android application (APP) with alkaline phosphatase (ALP)-triggered silicon nanodot (Si NDs) fluorescence biosensing to achieve field-deployable quantification. In the sensing system, ascorbic acid (AA), produced by ALP-catalyzed substrate 2-phospho- l -ascorbic acid trisodium salt (AA2P), can reduce the silane precursor 3-aminopropyltrimethoxysilane (APTMS) at room temperature to nucleate and grow green fluorescent Si NDs with an emission wavelength of 505 nm. The fluorescence intensity is dynamically modulated by the inhibitory effect of glyphosate on ALP activity, enabling a quantitative relationship between pesticide concentration and signal output. The sensor displayed a robust linear response toward glyphosate across 0.005–100 μM, with detection limits of 2.4 nM (fluorimeter) and 18 nM (APP). Importantly, the custom-designed Android application integrates image acquisition, RGB signal extraction, and calibration modeling, transforming smartphone into portable analytical devices. This synergy between nanomaterial innovation and digital technology not only delivered high recovery rates (80–109.5%) in spiked real samples but also demonstrated excellent reproducibility (RSD < 2.9%) and selectivity, underscoring its promise as a next-generation strategy for on-site glyphosate monitoring.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 2
·97
·
IF 5.3
Liu Bo,Han Qianxi,Wang Wenxuan,He Lijing,Wang Wenjie,Cheng Yanfen,Yun Shaojun,Cheng Feier,Feng Cuiping,Cao Jinling
Pyrethroid pesticide residues pose a significant global public health challenge, particularly in complex edible fungus matrices where trace, structurally similar pesticides are difficult to distinguish and detect. To address this critical gap, a novel surface-enhanced Raman spectroscopy (SERS) platform is presented integrating “magnetic enrichment, covalent organic framework (COF)-mediated capture, silver shell enhancement, and machine learning (ML)-driven intelligent recognition”. The core innovation lies in the tailored Fe₃O₄@COF@Ag nanocomposite architecture where the magnetic Fe₃O₄ core enables rapid sample separation, the COF intermediate layer provides specific molecular capture, and the Ag shell generates dense SERS “hotspots”, synergistically enhanced by ML algorithms to enable precise discrimination of structurally analogous pesticides. The developed Fe₃O₄@COF@Ag substrate demonstrated exceptional performance, achieving detection limits as low as 5.21 µg/kg for deltamethrin (CF), 6.08 µg/kg for fenvalerate (FV), and 11.6 µg/kg for lambda-cyhalothrin (LCF). The method exhibited outstanding linearity (R² > 0.99) for quantitative analysis and anti-interference capability, with recoveries of 94.36-106.89% in real samples. By integrating ML algorithms, the platform achieved 100% classification accuracy for distinguishing structurally similar pyrethroids. Principal component analysis (PCA) and support vector machine (SVM) models effectively extracted discriminative spectral features, enabling precise identification even at trace concentrations. This work provides a rapid, sensitive, and field-deployable solution for pesticide monitoring, offering significant potential to enhance food safety and public health protection. Graphical
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FOOD CHEMISTRY
·
2026
·
IF 9.8
Donglin Xiang,Xiao Wang,Chun Ji,Laping He,Yuangen Wu,Han Tao
This study presents a novel electrochemical strategy for detecting organophosphorus pesticides based on the synergistic catalytic of red kidney bean esterase (RKBE) and iron–cobalt dual-atom catalyst embedded in N-doped carbon carrier (FeCo–NC). RKBE was extracted from the widely cultivated British red kidney beans ( Phaseolus vulgaris L.) . FeCo–NC exhibited excellent electrocatalytic and peroxidase-like activity, catalyzing o -phenylenediamine oxidation to produce electroactive 2,3-diaminophenazine, which served as signal probe generating a sensitive cathodic current. Notably, RKBE product 1-naphthol inhibited FeCo–NC activity, while organophosphorus pesticides counteracted this inhibition by suppressing RKBE activity, restoring the electrochemical signal of 2,3-diaminophenazine. Leveraging this signal-regulation mechanism, a dichlorvos sensor was developed, with wide linear range (50–50,000 pM), low detection limit (14 pM), and excellent real-sample recovery (93.33%–107.08%), demonstrating practical application potential. This study provides an affordable and easy-to-operate approach for large-scale on-site monitoring of organophosphorus pesticide residues in foods.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Linlin Liu,Min Shi,Yanyan Wu,Jiali Hao,Junxiu Guo,Shanshan Li,Pingli Dai,Jing Gao
The widespread use of pesticides poses a significant threat to honeybee health by impacting their survival, behavior, immune function, and detoxification capacity. While phytochemicals such as resveratrol (RSV) have shown potential in mitigating oxidative stress and enhancing antioxidant defenses, their role in improving honeybee tolerance to pesticide exposure remains underexplored. In this study, we investigated the effects of RSV supplementation on honeybees exposed to three pesticides: dinotefuran (DIN), tebuconazole (TEB), and deltamethrin (DEL). The results showed that RSV supplementation significantly improved survival, feed intake, mobility, and gustatory sensitivity, indicating its protective effects against pesticide toxicity. Furthermore, RSV helped normalize impaired detoxification enzyme activities, including SOD, POD, catalase, and glutathione reductase, and reduced ROS levels and lipid peroxidation. Gene expression analysis revealed that RSV modulates Toll pathway-related genes like defensin and apidaecin , alleviating immune suppression caused by pesticides. Additionally, RSV influenced the insulin/insulin-like growth factor signaling (IIS) pathway by reducing ilp1 and inr1 expression, potentially mitigating metabolic stress. These findings demonstrate that protective effects of RSV may be linked to its ability to counter oxidative stress, restore mitochondrial function, and enhance energy metabolism. Furthermore, RSV is widely available, cost-effective, and easily incorporated into bee feed, making it feasible for large-scale application. This study highlights the protective role of RSV in pesticide detoxification in honeybees, offering new perspectives for honeybee health management and environmental toxicology research. By reducing the adverse effects of pesticides on honeybees, the application of RSV not only contributes to maintaining ecological balance but also supports sustainable agricultural practices. Future research should focus on optimizing its dosage, evaluating long-term effects, and investigating its impact on colony dynamics to facilitate its practical implementation in apiculture.
研究领域:
昆虫学
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环境毒理学
·
分子生物学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Aying Wen,Yao Xiong,Qiang Li,Qingrun Liu,Guoliang Li,Bing Shao
In this work, covalent organic framework (COF)-derived magnetic nanocomposites (Fe 3 O 4 @PDA@COF TABP ), comprising Fe 3 O 4 as the magnetic core, polydopamine (PDA) as the hydrophilic interlayer, and COF TABP as the outer shell, were first constructed and utilized as the powerful adsorbents for the magnetic solid-phase extraction (MSPE) of pyrethroids (PYRs). The introduction of the PDA coating facilitated the growth of the COF TABP shell and simultaneously provided additional adsorption sites, thereby enhancing the extraction efficiency. Molecular docking simulations revealed that Fe 3 O 4 @PDA@COF TABP exhibited excellent affinity toward PYRs through synergistic π-π stacking, hydrogen bonding, and hydrophobic interactions. The developed MSPE method coupled with GC-μECD exhibited excellent linearity (R 2 ≥ 0.9988), low limits of detection (0.025–0.064 μg kg −1 ), satisfactory recoveries (82.4 %–106.8 %), and low relative standard deviations (RSDs <7.1 %). Finally, the established method was successfully applied to the quantification of trace PYRs in vegetable samples, demonstrating its strong potential for routine pesticide residue monitoring in vegetables.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yahui He,Yajie Liu,Xinyu Liu,Yao Zhu,Yaling Yang,Jing Wang,Baoguo Sun
Due to its common use as an insecticide during agricultural production, residual lambda-cyhalothrin (LC) in food and the environment presents a significant human health risk. Developing an accurate LC detection method that does not require pretreatment remains a challenge. This study developed a novel yellow carbon dots molecularly imprinted (YCDs@MIPs) polyvinylidene fluoride (PVDF) composite (YCDs@MIPs@PVDF) fluorescent membrane for rapid visual LC detection. This method required no pretreatments during analysis since the LC could be specifically adsorbed when the samples passed through the YCDs@MIPs@PVDF membrane, causing fluorescence quenching, while matrix-interfering components could be removed for the porous structure of the membrane. A smartphone was used for rapid visual LC detection based on membrane fluorescence color changes in a dark-box ultraviolet analyzer. In food with a 15 % PVDF content and 6 mL added YCDs@MIPs, LC was quantitatively detected in a broad concentration range (5–500 μg/L) with a limit of detection (LOD) of 0.039 μg/L to 0.16 μg/L, which was lower than the strictest EU2019/92 limit of 10 μg/L. The analysis time was reduced from over 8 h when using the GB 23200.121–2021 and GB/T20769–2008 national standard methods to 30 min, while the analytical procedure decreased from six steps to two. The visual detection strategy combining the YCDs@MIPs@PVDF composite fluorescent membrane with a smartphone provided a new rapid detection technique for composite membrane purification and fluorescence signal output function.
研究领域:
食品安全
·
分析化学
·
环境科学
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Derong Lin,Yi Ma,Jiali Gu,Linhong Zou,Wen Qin,Zhijun Wu
In this study, a SiO 2 aerogel/chitosan/humic acid composite (SA-CS-HA) was synthesized through co-precipitation for efficient removal of deltamethrin (DM) from water, and its potential application in fruit and vegetable juices was explored. SA-CS-HA exhibited a predominantly microporous and mesoporous structure, with its specific surface area of 483.24 m 2 /g, its pore volume of 0.61 cc/g, and its pore diameter of 1.43 nm. The FT-IR analysis revealed that SA-CS-HA contained abundant reactive functional groups, including carboxyl, hydroxyl, and aromatic compounds. And the thermal stability of composite SA-CS-HA was enhanced. The optimal conditions for DM absorption by SA-CS-HA were determined as followers: a temperature of 30 ℃, DM concentration of 20 mg/L, pH of 4, adsorbent dosage of 4 mg, and the adsorption time of 600 min. The kinetics study showed that the adsorption data conform to the pseudo-second-order kinetic model; the Langmuir model suggested that the monolayer adsorption occurred on the surface of SA-CS-HA, with a maximum adsorption capacity of 209.52 mg/g at 30 ℃; the Dubinin-Radushkevich model indicated that adsorption was a physical process; the thermodynamics analysis affirmed the spontaneous nature of adsorption, with its physicochemical properties not affected during application to fruit and vegetable juices. Additionally, the study of the adsorption mechanism of SA-CS-HA highlighted the significant role of electrostatic attraction, accompanied by hydrogen bonding interactions involving oxygen-containing functional groups such as -OH and -COOH in SA-CS-HA.
研究领域:
环境科学
·
材料科学
·
化学工程
·
水处理
·
食品科学
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高影响因子
危险品化学品经营许可证（带存储）