Food Bioscience
·
2025
·
IF 5.9
Chen Siwei,Huang Jialiang,Liu Heng,Zhang Xiao,Xu Ting,Luo Lin,Chen Sui,Xu Zhenlin,Zhou Kai
Glutaminase plays a crucial role in nitrogen metabolism and is of vital significance for soy sauce production. In this study, the high glutaminase-producing strains, Bacillus pumilus -T1-11 and Bacillus safensis -T3-20, were isolated from fermented moromi through a two-step screening process. T1-11 displayed intracellular glutaminase activity of 146.39 ± 8.14 U/g, while T3-20 presented 139.26 ± 15.62 U/g, and both strains maintaining activity in Secondary Screening medium(SRM). Culture conditions were optimized by adjusting nitrogen/carbon sources, glutamine, NaCl, and pH. T1-11 achieved the maximum activity of 5716.95 ± 168.18 U/g in optimized medium and T3-20 reached peak activity of 4927.72 ± 62.79 U/g. The application of T1-11 and its enzyme extracts in soy sauce fermentation was evaluated. The moromi fermented with 7.0 log CFU/mL inoculum for 2 d showed the peak glutaminase activity (98.99 ± 15.13 U/g), significantly enhancing umami flavor, as confirmed by sensory and biochemical analyses. In extracted soy sauce, a 6.0 log CFU/mL inoculum cultured for 14 d total glutamic acid content to 82.78 ± 0.53 mmol/L. These modifications significantly enhanced the umami characteristics of soy sauce, demonstrating the potential application of this strain in improving the quality of traditional fermented food .
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2026
·
IF 7.3
Xin Zhang,Dapeng Li,Ke Jiang,Chao Zeng,Mi He,Xiancheng Ma,Chongshan Yang,Ling Li,Tao Wen
Early detection of Bactrocera dorsalis ( B. dorsalis ) infestation in citrus is challenging due to the asymptomatic nature of initial larval stages. Colorimetric sensor arrays (CSAs) are promising for non-destructive detection of B. dorsalis infestations in citrus, yet they still face limitations in reliably identifying the key volatile organic compounds (VOCs) released by infested citrus. In this study, we developed a MOF-enhanced colorimetric sensor array integrating pH-sensitive dyes into ZIF-7 nanoparticles for non-invasive detection. The array detected VOCs associated with early infestation, including D -limonene, decanal, and linalool, identified via GC-MS. The dye/ZIF-7 composite CSA exhibited enhanced sensitivity compared to the pure dye sensor array. Furthermore, across the 4-day test period, infested samples registered a progressive rise in total Euclidean distance (ED) of color change, reaching a 171.7-unit increase over healthy controls on day 4. To enhance the accuracy and efficiency of infestation detection, we implemented a machine-learning pipeline using Dung Beetle Optimizer (DBO) for feature selection and Tree-structured Parzen Estimator (TPE) for hyperparameter tuning. Consequently, the XGBoost classifier achieved a peak test accuracy of 97.15 %, enabling reliable detection during the early stage of infestation. Overall, this study offers a non-destructive and cost-effective approach for batch-mode early detection of B. dorsalis infestation in citrus, advancing the potential for enhanced postharvest monitoring and pest management strategies.
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Journal of Physical Chemistry Letters
·
2026
·
IF 4.5
Yuting Sun,Qingyuan Zhao,Xiaoying Yu,Aijun Li,Hitoshi Tamiaki,Shin-ichi Sasaki,Yanhui Wang,Xiao-Feng Wang
Conventional photodetectors are typically limited to monochromatic image sensing, which is increasingly inadequate for modern applications. In this work, we demonstrate full-color image sensing using an organic photodetector (OPD) based on a ternary heterojunction comprising 131-deoxo-131-dicyanomethylene-pyropheophorbide-a (Chl), poly(3-hexylthiophene-2,5-diyl) (P3HT), and [6,6]-phenyl-C61-butyric acid methyl ester (PC61BM). Compared with the PC61BM:P3HT device, incorporating Chl effectively enhances the external quantum efficiency. This is mainly attributed to the lowest unoccupied molecular orbital and highest occupied molecular orbital energy levels of Chl lying between those of PC61BM and P3HT, which facilitates efficient extraction of electrons and holes. Moreover, by leveraging the device’s distinct responses at 475, 530, and 730 nm, color image sensing is realized in the self-powered mode, with the reconstructed images achieving a structural similarity index of up to 97% based on external RGB reconstruction. This study offers a new strategy for developing flexible and high-performance color imaging devices.
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Nano-Micro Letters
·
2026
·
IF 38.5
Ming Chen,Qinglong Luo,Xiaolei Wang,Kaiyuan Shi
The increasing demand for high-performance energy devices has prompted the exploration of advanced electrolytic solutions for aqueous energy storage. Redox-enhanced Zn-ion capacitors (RZICs) overcome the limitations of conventional electrochemical capacitors by integrating redox-active molecules into the electrolyte, which enables higher energy density and expanded voltage windows. In this study, we developed organic dye-based colorimetric indicators for the fabrication of functional electrolytes in RZICs. The structural responsiveness of these dyes, driven by proton–electron transfer through electrochromic dynamics, allows real-time monitoring and optimization of the RZICs. The acid–base equilibrium of colorimetric indicators supports pH buffering, resulting in an extended lifespan of Zn||Zn cells up to 4,000 h. The conjugated aromatic structure of the indicators enhances their adsorption onto activated carbon, thereby minimizing the self-discharge in RZICs. Additionally, the phenol–quinone transformation increases the capacity of RZICs to 152.4 mAh g −1 within an optimized voltage window of 0.2–1.6 V, while promoting electrochemical kinetics and suppressing anode degradation. The results advance the design and customization of redox electrolytes with colorimetric properties for supercapacitive energy storage.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Jianli Zhou,Chuqi Tang,Shuliang Zou,Liangbo Lei,Yuangen Wu,Wenhua Yang,Jean Damascene Harindintwali,Jiang Zhang,Wenwen Zeng,Dan Deng,Manman Zhao,Xiaobin Yu,Xiaobo Liu,Shuyi Qiu,Nils Arneborg
The development of blueberry wine provides an alternative method for maintaining the nutritional value and extending the shelf life of blueberries. However, anthocyanin loss and off-flavor compound generation during fermentation impair blueberry wine color and quality. Hydroxycinnamate decarboxylase from yeast can catalyze the conversion of hydroxycinnamic acids to vinylphenols, which later may condense with anthocyanins to form more stable vinylphenolic pyranoanthocyanins. In this study, 10 non- Saccharomyces yeasts from Daqu that showed hydroxycinnamate decarboxylase activity were screened. Among the 10 strains, Wickerhamomyces anomalus Y5 showed the highest consumption (34.59%) of the total tested phenolic acids and almost no H 2 S production. Furthermore, Y5 seemed to produce four vinylphenol pyranoanthocyanins (cyanidin-3-O-galactoside/glucoside-4-vinylcatechol, cyanidin-3-O-galactoside/glucoside-4-vinylsyringol, malvidin-4-vinylguaiacol, and malvidin-4-vinylcatechol) during blueberry wine fermentation, which may improve the color stability of blueberry wine. These findings provide new insights for improving the quality of blueberry wine using non- Saccharomyces yeasts.
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JOURNAL OF APPLIED MICROBIOLOGY
·
2021
·Vol. 131
·期 4
·1890-1908
·
IF 3.772
Win T.T.,Bo B.,Malec P.,Fu P.
AimsThis study sought to utilize indigenous soil micro‐organisms to suppress wilt‐causing fungal pathogens of the banana.Methods and ResultsFungal pathogens were isolated from wilt‐affected rhizospheric soil, and potential antagonistic bacterial strains were isolated from healthy rhizospheric soil in the same area from which fungal pathogens were isolated. The antifungal activity of isolated micro‐organisms against fungal pathogens was studied both in vitro and in vivo against fungal pathogens. It was found that Fusarium oxysporum and Alternaria sp. were pathogenic, while Penicillium sp., Bacillus velezensis and Bacillus subtilis were antagonistic. Moreover, it was seen that B. velezensis, B. subtilis and Penicillium sp. inhibited the growth of the two fungal pathogens in both in vitro and in vivo experiments. Further investigation indicated that B. velezensis, B. subtilis and Penicillium sp. were able to produce enzymatic antifungal compounds (chitinase and β‐1,3‐glucanase). The spray application around rhizome revealed that a combination of Bacillus spp. and Penicillium sp. in greenhouse conditions gave the highest reduction in disease severity by up to 60% to both fungal pathogens among the treatments.ConclusionsBanana disease is seen to be induced not only by F. oxysporum but also by Alternaria sp. The isolated indigenous micro‐organisms can effectively control both the pathogens. The combination of isolated antagonistic micro‐organisms has thus demonstrated substantial potential for suppressing banana disease.Significance and Impact of the StudyAn antagonistic consortium isolated in this study has demonstrated remarkable potential for controlling fungal diseases caused by Fusarium sp. and Alternaria sp. Therefore, the use of indigenous microflora to improve disease suppression of banana plants against soil‐borne pathogens is a preferable approach.
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ACS Sensors
·
2021
·
IF 7.711
Kuikui Zhang,Jiaxue Zhang,Fenfang Wang,Desheng Kong
Stretchable and wearable sensors allow intimate integration with the human body for health and fitness monitoring. In addition to the acquisition of various physical parameters, quantitative analysis of chemical biomarkers present in sweat may provide vital insights into the physiological state of an individual. A widely investigated system utilizes electrochemical techniques for continuous monitoring of these biomarkers. The required supporting electronics and batteries are often challenging to form a deformable system. In this study, an intrinsically stretchable sensing patch is developed with compliant mechanical properties for conformal attachment to the skin and reliable collection of sweat. In these patches, superhydrophilic colorimetric assays consisting of thermoplastic polyurethane nanofiber textiles decorated with silica nanoparticles are assembled over a styrene–ethylene–butylene–styrene-based superhydrophobic substrate, thereby generating a large wettability contrast to efficiently concentrate the sweat. The system supports multiplexed colorimetric analysis of sweat to quantify pH and ion concentrations with images acquired using smartphones, in which the influence of ambient lighting conditions is largely compensated with a set of reference color markers. Successful demonstrations of insitu analysis of sweat after physical exercises effectively illustrate the practical suitability of the sensing patch, which is attractive for advanced health monitoring, clinical diagnostics, and competitive sports.
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MACROMOLECULES
·
2021
·
IF 5.985
Chao Nan Zhu,Si Yu Zheng,Hao Nan Qiu,Cong Du,Miao Du,Zi Liang Wu,Qiang Zheng
Developing hydrogels with new structures and extraordinary performances is fundamental and mission-critical for the advancements of gel materials. Here, we report a class of tough supramolecular hydrogels facilely prepared by polymerizing methacrylic acid precursor solution in the presence of hexadecyltrimethylammonium chloride micelles. After swelling the as-prepared hydrogels in water, strong polyelectrolyte/surfactant complexes (PESCs) are formed between the weakly charged polymer chains and oppositely charged surfactants, serving as the physical cross-links of the gel matrix. The equilibrated hydrogels are transparent with a water content of 50–85 wt % and possess excellent mechanical properties, with a tensile breaking stress of 0.1–5 MPa, a breaking strain of 600–1200%, and Young’s modulus of 1–70 MPa. Typical yielding is observed at a small tensile strain of ∼10%, followed by forced elastic deformation of the hydrogels, which are in a glassy state due to the reduced segmental mobility of the matrix highly cross-linked by PESCs. The plastic-like mechanical properties of hydrogels could be well tuned by pH, temperature, and ionic strength that influence the ionization of polymer chains and the strength of PESCs. These dynamic behaviors render the hydrogels with self-recovery and shape-memory properties. Furthermore, the nanosized hydrophobic pockets within the hydrogels afford the capacity of loading functional hydrophobic molecules with promising applications as fluorescent materials and drug delivery systems. The design principle and strategy should be extended to other systems, including biomacromolecules and lipids, toward broad applications in biomedical and engineering fields.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Junfan Niu,Gang Tang,Jingyue Tang,Jiale Yang,Zhiyuan Zhou,Yunhao Gao,Xi Chen,Yuyang Tian,Yan Li,Jianqiang Li,Yongsong Cao
Silver nanoparticles (AgNPs) have remarkable and broad-spectrum antibacterial activities against Gram-positive (G+) and Gram-negative bacteria (G−). However, the negative surface potential of AgNPs limits their antibacterial activities due to the electrostatic repulsion with the negatively charged bacterial cell membrane. To address the limitation, AgNPs were loaded in the mesoporous silica nanoparticles by preparing silver core–mesoporous silica shell nanocapsules (Ag@MSNs), and then, a cationic antibacterial polymer, quaternary ammonium polyethyleneimine (QPEI), was used to modify Ag@MSNs for improving their surface potential and antibacterial activities. The results showed that the obtained Ag@MSN-QPEI exhibited a high positive surface potential (+39.6 mV) and a strong electrostatic attraction with Pseudomonas syringaepv. lachrymans cells in coculture, resulting in an excellent bacterial cell-targeting effect. At the same concentration, Ag@MSN-QPEI exhibited less silver content (reducing the silver content of Ag@MSNs by 19%), higher antibacterial activities, and longer effective duration against Clavibacter michiganensis subsp. michiganensis (G+) and P. syringaepv. lachrymans (G−) than Ag@MSNs and QPEI alone. The excellent bacterial cell-targeting effect and synergistic antibacterial action combined with QPEI accounted for the significantly enhanced antibacterial activities of Ag@MSN-QPEI. Therefore, using a cationic antibacterial polymer to confer the bacterial cell-targeting effect and synergistic antibacterial action would be extended to other antimicrobial materials.
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E-POLYMERS
·
2021
·Vol. 21
·期 1
·500-510
·
IF 2.025
Xiaoguang Ying,Jieyuan He,Xiao Li
An imprinted electrospun fiber membrane was developed for the detection of volatile organic acids, which are key components of human body odor. In this study, hexanoic acid (HA) was selected as the target, polymethyl methacrylate (PMMA) was used as the substrate, and colorimetric detection of HA was achieved by a bromocresol purple (BCP) chromogenic agent. The results showed that the morphology of the fiber membrane was uniform and continuous, and it showed excellent selectivity and specificity to HA. Photographs of the color changes before and after fiber membrane adsorption were recorded by a camera and quantified by ImageJ software by the difference in gray value (ΔGray). This method is simple, intuitive, and low cost and has great potential for application in human odor analysis.
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Advanced Electronic Materials
·
2018
·Vol. 4
·期 11
·1800237
·
IF 5.466
Xin Wang,Yao Wu,Guanwen Li,Jun Wu,XiaoBing Zhang,Qing Li,Baoping Wang,Jing Chen,Wei Lei
Organometallic perovskites are candidates for low-dose ionizing radiation due to their large mobility-lifetime product, large radiation absorption coefficient, high stability at ambient temperature, and ability to crystallize from solution at low temperatures. However, multiple unstable and expansive organic layers are required to block injected charges from the applied voltage. Here, a radiation photodiode based on p–n junctions formed in the CH 3 NH 3 PbI 3 crystallization process without any other organic layers is reported. Through controlling the location of the dopant, selenium (Se), and its concentration inside CH 3 NH 3 PbI 3 single crystals, the valence band offset of the band alignment is measured as 0 to 0.45 eV. The photodiodes require only two gold electrodes deposited on opposite faces of the CH 3 NH 3 PbI 3 single crystals, and the sensitivity for 60 keV X-ray and 1.25 MeV gamma-ray from Cobalt-60 reached 21 µC mGy air cm −2 and 41 µC Gy air cm −2 , respectively. Experiments measured the ultrafast response time to be 3 µs.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yixuan Pan,Maohua Yang,Mei Zhang,Mingyan Jia
American ginseng and Asian ginseng products have similar appearance, however, the huge price differences for various species and grades of ginseng lead to the widespread adulteration in the commercial market. We herein reported an efficient colorimetric sensor array for ginseng discrimination through analysis of diols composition (e.g. monosaccharide and ginsenoside). The sensor array was rationally designed based on two mechanisms, changes in pH and indicators displacement assay (IDA), with phenylboronic acid as the receptor to diols. The excellent discrimination capability of the sensor array was validated upon analysis of different species or concentrations of monosaccharides in ginseng hydrolysate. 40 ginseng samples within three categories (cultivated American ginseng, wild American ginseng and Asian ginseng) were successfully classified using the sensor array coupled with a statistical analysis such as hierarchy cluster analysis (HCA) and principal component analysis (PCA). Our work provided a simple and effective method for the fast discrimination and quality control of ginseng products.
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