Current Nanoscience
·
2012
·Vol. 8
·期 1
·130-140
·
IF 1.776
Kumar Umesh,K. Ranjan Amaresh,Sharan Chandrashekhar,A. Hardikar Anandwardhan,Pundle Archana,Poddar Pankaj
Inspired by array of proteins present in nature, we choose lysozyme (hen egg protein) as a model system to synthesize nanoparticles of noble metals to understand the mechanism of interaction as well as to use them for potential applications such as potent antibacterial agents. Lysozyme
is a very well studied biomolecule containing aromatic amino acids like tryptophan and tyrosine. Tyrosine has phenoxy group which is considered to be responsible for interacting with the metal ions. Lysozyme can be suitably modified by treatment with N-bromosuccinimide/N-acetylimidazole to
obtain tight control over size distribution of nanoparticles. Here we report the direct synthesis of nanocrystals of gold and silver at controlled pH and light conditions without using any known reducing agents. Out of these, synthesis of gold nanoparticles is assisted by the presence of low
concentration of Ag+ ions through the galvanic exchange. It is remarkable to note that the structure of protein is not changed drastically as seen by the FTIR studies. As-synthesized lysozyme capped nanoparticles prepared by this method are biocompatible and retain antibacterial property.
研究领域:
生物化学
·
纳米生物学
·
材料科学
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DOI
高影响因子
Processes
·
2023
·Vol. 11
·期 9
·2644
·
IF 3.5
Shanshan Yang,Lixia Hou,Min Su
Protein crystallization is a widely employed technique for purifying protein drugs, offering notable benefits such as cost-effectiveness and high purity. However, the success of this method is influenced by factors such as the molecular weight and spatial structure of proteins. The challenges associated with achieving crystallization and the prolonged duration required for crystallization induction pose limitations on its widespread industrial implementation. In this study, we employed lysozyme derived from egg white as a representative protein to investigate the polymer-assisted self-assembly of magnetic lysozyme. Through the optimization of the initial interstitial crystallization process of magnetic lysozyme, we manipulated the supersaturation level of lysozyme and applied magnetic nanoparticle treatment. As a result, we successfully reduced the crystallization time from 24 h to 60 min. Subsequently, the findings derived from the analysis of data pertaining to the interstitial crystallization process of lysozyme were utilized to optimize the design and configuration of a push flow crystallizer (PFC) as well as a slug flow crystallizer (SFC). The analysis encompassed the examination of various factors, including the residence time of crystallization, the yield of the process, the shape of the crystals formed, and the distribution of crystal sizes. Ultimately, it was determined that the SFC demonstrated optimal suitability for the crystallization of magnetic lysozyme. The typical V-PFC crystal size is 16 m and the yield is 60%. V-SFC crystals have an average size of 13 m and a yield of 85%.Keywords:lysozyme;continuous crystallisation;tubular;magnetic
研究领域:
生物化学
·
制药工程
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产品关联
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2020
·
IF 4.499
Ying Ji
The combination of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidization and annealing was applied to modify structural properties of corn starch in order to obtain starch polymer microgels. Microgels were characterized by X-ray diffraction, ATR-FTIR and thermogravimetry analysis (TGA). The effects of microgels on the uptake and release of lysozyme were also analyzed. The formation of oxidized microgels through the crosslinking between starch molecules and sodium trimetaphosphate was confirmed by X-ray diffraction. The ATR-FTIR analysis suggested that TEMPO-oxidation results in the formation of carboxyl groups. TGA data indicated that oxidized-annealed microgels had less thermal stability than oxidized microgels. Furthermore, the oxidized-annealed microgels had an improved capacity for uptake and release of lysozyme. At this stage of the exploration, microgels prepared from corn starches by annealing and oxidation is simple and efficient.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 10
·1931
·
IF 5.2
Dashan Guo,Yuwei Hou,Hongshan Liang,Lingyu Han,Bin Li,Bin Zhou
The distinctive assembly behaviors of lysozyme (Lys) feature prominently in food, materials, biomedicine, and other fields and have intrigued many scholars. Although our previous work suggested that reduced glutathione (GSH) could induce lysozyme to form interfacial films at the air/water interface, the underlying mechanism is still obscure. In the present study, the effects of GSH on the disulfide bond and protein conformation of lysozyme were investigated by fluorescence spectroscopy, circular dichroism spectroscopy, and infrared spectroscopy. The findings demonstrated that GSH was able to break the disulfide bond in lysozyme molecules through the sulfhydryl/disulfide bond exchange reaction, thereby unraveling the lysozyme. The β-sheet structure of lysozyme expanded significantly, while the contents of α-helix and β-turn decreased. Furthermore, the interfacial tension and morphology analysis supported that the unfolded lysozyme tended to arrange macroscopic interfacial films at the air/water interface. It was found that pH and GSH concentrations had an impact on the aforementioned processes, with higher pH or GSH levels having a positive effect. This paper on the exploration of the mechanism of GSH-induced lysozyme interface assembly and the development of lysozyme-based green coatings has better instructive significance.Keywords:reduced glutathione;lysozyme;defolding;self-assembly
研究领域:
食品科学
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材料科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Xiang Zou,Junguo He,Xinlei Pan,Qiupeng Cai,Shengye Duan,Yijie Zhong,Xinxin Cui,Jie Zhang
This study investigated the impact of humic acid (HA) on enzymatic pretreatment efficiency, focusing on sludge properties and HA molecular structure. The results showed that enzymatic pretreatment alleviates HA inhibition, improving hydrolysis efficiency. In the presence of HA, soluble proteins and polysaccharides in the enzyme-cocktail group reached 27.7 mg/L and 23.9 mg/L, 1.4 and 1.3 times higher than the blank group, respectively. The enzyme-cocktail group also had the highest soluble DNA concentration (19.4 mg/L) and the lowest viable cell proportion (69.3 %), indicating effective cell lysis. Enzyme-cocktail pretreatment reduced electrostatic repulsion, enhancing the mobility of extracellular organics. Enzyme interactions with HA released internal hydrolases and decreased amide groups on the HA surface, increasing the availability of biodegradable substrates. Overall, enzymatic pretreatment proves effective in mitigating HA-induced inhibition, thereby improving sludge biodegradation and enhancing carbon recovery in anaerobic fermentation.
研究领域:
环境生物学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2021
·
IF 3.317
Fei Yu,Yafei Mao,Hongtu Zhao,Xiunan Zhang,Ting Wang,Mingpu Yuan,Suping Ding,Na Wang,Xin Huang,Hongxun Hao
Protein drugs have attracted extensive attention from the academic community. However, crystallization of proteins is difficult and the yield of current main technologies is too low, which limits their large-scale industrial production. In this work, enhancement of continuous crystallization of lysozyme under an ultrasonic field was explored. A plug-flow tubular crystallizer, which was separated into a nucleation zone and a growth zone, was applied. The effect of ultrasound on steady-state time, crystal habit, particle size, particle size distribution, and the process yield was evaluated based on univariate analysis experiments. It was found that ultrasound can shorten the time for continuous crystallization to reach a steady state, reduce the degree of aggregation, and promote nucleation. Furthermore, the separation of the growth zone from the nucleation zone in the ultrasonic-assisted continuous crystallization can further increase the size of the lysozyme crystals, which would help in increasing the yield.
研究领域:
生物化学
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制药工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Dan Zhao,Xiaoyun Li,Mengyu Xu,Yan Jiao,Huan Liu,Xincai Xiao,Haiyan Zhao
The preparation of functional long-wavelength-emitting nanomaterials and the researches on their applications in antibacterial and antibiofilm fields have important significance. This paper reports the preparation of yellow-green-fluorescent and high- quantum yield carbon dots (4-ACDs) with 4-aminosalicylic acid and polyethylene imine as raw materials through one-step route, and the impacts of raw material structure and the reaction conditions upon the optical properties of the products have been investigated. 4-ACDs exhibit excellent broad-spectrum antibacterial activity , and their good biocompatibility ensures them as ideal fluorescent nano-probe for cell imaging . However, 4-ACDs could not effectively eliminate the biofilm of Staphylococcus aureus ( S. aureus ). CDs-LZM complex was prepared through the coupling between 4-ACDs and lysozyme (LZM) and the complex showed strong antibacterial activity against Gram-positive bacteria, particularly with MIC against S. aureus at 5 μg mL −1 . Besides, CDs-LZM showed excellent ability against the biofilm of S. aureus . At the concentration of 60 μg mL −1 , its inhibition rate against the growth of biofilm was 86 %, and elimination rate against biofilm reached 76 %. CDs-LZM exhibited obvious antibiofilm ability through removing extracellular matrix of biofilm, greatly reducing the thickness of biofilm under confocal microscopy . The application of novel long-wavelength-emitting nanomaterial in eliminating pathogenic bacteria is of great significance.
研究领域:
生物医学
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纳米材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR RAPID COMMUNICATIONS
·
2024
·
IF 4.2
Na Feng,Shuting Miao,Xin Guo,Ziyi Yang,Luke Yan,Peng Yang,Jia Kong
Biomimetic slippery liquid-infused porous surfaces (SLIPS) have emerged as a promising solution to solve the limitations of superhydrophobic surfaces, such as inadequate durability in corrosion protection and a propensity for frosting. However, the challenge of ensuring strong, lasting adhesion on diverse materials to enhance the durability of the lubricant layer remains. The research addresses this by leveraging amyloid phase-transitioned lysozyme (PTL) as an adhesive interlayer, conferring stable attachment of SLIPS across a variety of substrates, including metals, inorganics, and polymers. The silica-textured interface robustly secures the lubricant with a notably low sliding angle of 1.15°. PTL-mediated adhesion fortifies the silicone oil attachment to the substrate, ensuring the retention of its repellent efficacy amidst mechanical stressors like ultrasonication, water scrubbing, and centrifugation. The integration of robust adhesion, cross-substrate compatibility, and durability under stress affords the PTL-modified SLIPS exceptional anti-fouling, anti-icing, and anti-corrosion properties, marking it as a leading solution for advanced protective applications.
研究领域:
材料科学
·
生物化学
·
表面工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jia Xu,Qing Wu,Jing Wang,Yingjie Liu,Ke Liu,Ming Xia,Dong Wang
The development of materials with high lysozyme adsorption is critical for drug delivery and skin wound applications, as it enhances antibacterial properties, stability, and controlled release of therapeutic agents, thereby improving treatment efficacy and safety. Alginate-based nanofiber scaffolds, featuring high surface area and multiple adsorption sites, can efficiently absorb lysozyme and regulate its release through tunable pore channels, offering a promising approach to chronic wound management. In this study, we fabricated poly (vinyl al co hol-co-ethylene) (EVOH) nanofiber-based sodium alginate (ENSA) aerogels using a simple two-step crosslinking procedure. The resulting aerogels, with controllable porosity formed via high-pressure spraying techniques (aerogel film) and molding (aerogel sponge), were evaluated for their high-loading capacity and controllable release of lysozyme. The aerogel film exhibited a remarkable lysozyme adsorption capacity of 1965 ± 36 mg/g, while the aerogel sponge sustained lysozyme release over 14 days. Analysis of the drug-release mechanism through four kinetic models revealed two distinct processes: cation exchange and matrix diffusion. The aerogel's pore structure influenced the diffusion processes, enabling tailored drug release profiles. Additionally, the ENSA aerogels demonstrated good mechanical properties, non-cytotoxicity, and potent antibacterial activity, positioning them as promising materials for skin wound dressings.
研究领域:
材料科学
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生物医学工程
·
药剂学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 138
·期 37
·50954
·
IF 3.125
Xing Zhou,Renfang Song,Dong Wang,Changqing Fang,Yonghua Song,Ruien Yu,Qiang Wang,Jingrui Deng
The integration of biological matters and functional polymers to generate novel hybrids with multi-functional properties has attracted an increasing attention in organic/organic systems, possessing a great promise for sensor, medical, and materials science applications. In this work, a facile co-precipitation method was developed to prepare conductive composite via a co-assembly process with one-step. Lysozyme was employed as an assembly template, and the pyrrole monomers were polymerized and aggregated with the lysozyme nanoparticles via oxide polymerization, forming a hybrid and functional coating with significant conductivity of ca. 6.7 × 10 −5 S. The structure and morphology of the hybrid is performed by X-ray photoelectron spectroscopy, Fourier transform infrared spectrometer, transmission electron microscopy, field emission scanning electron microscope, and laser scanning confocal microscope, and so on. The results showed that the coating was composed of homogenously and densely hybrid nanoparticles with the diameter of ca. 250 nm, possessing a certain degree of bio-adhesion from the lysozyme precipitations according to the 3 M peeling test. In addition, the composite performed good infrared radiation shielding property when it was coated onto normal gauze. It suggests that the composite consisting of lysozyme and polypyrrole precipitations in nanoscale may possess significantly potential applications as conductive and shielding materials.
研究领域:
材料科学
·
生物医学工程
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2021
·Vol. 14
·期 7
·1464-1471
·
IF 3.366
Zhang Qianchun,Chen Junyu,Wang Yutong,Xia Guangping,Zheng Yuguo,Wang Xingyi,Wu Yun,Zhang Changbo
A novel aptamer solid-phase microextraction (SPME) fibre-based on poly (methacrylic acid-co-ethylene glycol dimethacrylate) for lysozyme-specific recognition has been successfully prepared. The prepared SPME fibres were characterised by various instrumental methods, including scanning electron microscopy, X-ray photoelectron spectroscopy and Fourier-transform infrared spectrometry. The results showed that the SPME fibre exhibited a porous structure, which is beneficial for the enrichment of the aptamer. The solid-phase microextraction-high-performance liquid chromatography-fluorescence detection (SPME-HPLC-FLD) with the superior selectivity and sensitivity responded linearly to the concentration of lysozyme over the 0.025–10 mg/L range, and the limit of detection was low at 6.8 μg/L, with a linearity of 0.9995. The method showed excellent reproducibility, and the relative standard deviations (RSDs) were less than 5.5%, which facilitated the analysis. The method was applied for the analysis of lysozyme in cow milk and papaya samples, and the recoveries ranged from 86.7–93.5% with RSDs of 4.3–6.1%, which clearly state that the proposed lysozyme aptamer SPME-HPLC-FLD method is a promising analytical tool for the analysis of lysozyme in food samples. Graphical abstract
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
·
IF 2.983
Wei Gao,Xiao-lan Liu,Jia-yuan Li,Hong-zhen Lian,Li Mao
A porous zwitterionic monolithic column was prepared to rapidly and efficiently separate lysozyme from egg white. The monolith was synthesized in a stainless steel HPLC column (5 cm × 4.6 mm i.d.) by in-situ thermal initiated co-polymerization of N,N- dimethyl -N- methacryloxyethyl- N -(3-sulfopropyl) ammonium betaine (MSA) and ethylene dimethacrylate (EDMA). Due to the combination of quaternary ammonium and sulfonic groups on the monolithic matrix in one-pot process, the hydrophobic carbon chain and hydrophilic radical were obtained, which provided multiple driving forces for neutral, basic and acidic analytes, thus mix-mode chromatography mechanism contributed to the retention of different charged proteins. Properties such as composition, morphology and stability of the MSA-co-EDMA monolithic column were characterized by various analytical methods and the results showed that the monolith has large through-pores, good hydrophilicity and permeability. The effects of mobile phase pH and ionic strength on proteins were investigated, drawing the conclusion that the main adsorption and elution mechanism of lysozyme on MSA-co-EDMA monolith was electrostatic interaction, while those of other proteins included hydrophobic, hydrophilic and electrostatic interactions. Therefore, efficient separation of lysozyme and other proteins could be successfully achieved by switching the pH of mobile phase. Lysozyme can be adsorbed using 20 mmol/L phosphate buffer (pH 7.0) and eluted with 20 mmol/L phosphate buffer (pH 2.0). To prove the practicality of the monolithic column, it was also applied in the separation of lysozyme in egg white, which means the work has the potential for further development in proteome analysis of real biological samples.
研究领域:
生物化学
·
蛋白质组学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）