JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Xiaoxiao Gu,Dongmei Li,Haoyang Yuan,Cailing Li,Dinghua Yu,Guowei Wang,Shuang Li
Baicalin is a flavonoid compound extracted from the Scutellaria baicalensis plant. It possesses strong free radical scavenging ability and has a wide range of medical applications in antibacterial, anti-inflammatory, and anti-tumor fields. However, its limited solubility in water restricts its application. In this study, we prepared SL-DMEA by the amidation of acidic sophorolipids and N, N-dimethylethanolamine, while SL-TMEAI was synthesized via the quaternization of SL-DMEA with iodomethane. The structures of SL-DMEA and SL-TMEAI were confirmed by 1 H NMR, FT-IR, and Mass spectra. Surface tension and micelle microenvironment indicated that SL-DMEA and SL-TMEAI exhibit superior surface activity. Furthermore, the solubility of baicalin in aqueous solutions containing SL-DMEA and SL-TMEAI was approximately 70 mg/L and 75 mg/L, while it was only 18 mg/L in SL-COOH solution, which were significantly higher than the solubility in pure water (4 mg/L). Similarly, SL-DMEA and SL-TMEAI can enhance the antioxidant activity of baicalin. Specifically, in the SL-TMEAI aqueous solution, the free radical scavenging rate of baicalin was increased by 10 %–20 %. Density functional theory calculations revealed that SL-DMEA and SL-TMEAI formed stable hydrogen bonds with baicalin molecules, which can increase the solubility of baicalin in water and expose the groups that react with free radicals in the solution, thereby enhancing the antioxidant activity. These results demonstrated that SL-DMEA and SL-TMEAI could significantly enhance the solubility and antioxidating of baicalin in water through a hydrotropy mechanism.
研究领域:
生物化学
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药物化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2024
·Vol. 108
·期 1
·1-12
·
IF 3.9
Lin Yilin,Fang Meimei,Liu Jun,Zhang Yehui,Yu Yigang
Abstract Phenyllactic acid (PLA) generally recognized as a natural organic acid shows against Vibrio parahaemolyticus activity. In this study, V. parahaemolyticus ATCC17802 ( Vp 17802) was cultured under the stress of 1/2MIC PLA, and then the antibacterial mechanisms were explored via transcriptomics. The minimum inhibitory concentration (MIC) of PLA against Vp 17802 was 3.2 mg/mL, and the time-kill analysis resulted that Vp 17802 was inhibited. PLA was able to destroy the bacterial membrane, leading to the leakage of intracellular substances and decline of ATP levels. The RNA-sequencing analysis results indicated that 1616 significantly differentially expressed genes were identified, among which 190 were up-regulated and 1426 were down-regulated. Down-regulation of the icd2 gene in the TCA cycle mediates blockage of tyrosine metabolic, arginine biosynthesis, and oxidative phosphorylation, causing insufficient energy supply of Vp 17802. Moreover, PLA could cause amino acids, metal ions, and phosphate transporters to be blocked, affecting the acquisition of nutrients. The treatment by PLA altered the expression of genes encoding functions involved in quorum sensing, flagellar assembly, and cell chemotaxis pathway, which may be interfering with the biofilm formation in Vp 17802, reducing cell motility. Overall, 1.6 mg/mL PLA inhibited the growth of Vp 17802 by disrupting to uptake of nutrients, cell metabolism, and the formation of biofilms. The results suggested a new direction for exploring the activity of PLA against Vp 17802 and provided a theoretical basis for bacterial pathogen control in the food industry. Key points •RNA sequencing was carried out to indicate the antibacterial mechanism of Vp17802. •The icd2 gene in the TCA cycle mediates blockage of metabolic of Vp17802. •The biofilm formation has interfered with 1.6 mg/mL PLA, which could reduce cell motility and virulence. Graphical Abstract
研究领域:
微生物学
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食品科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomedical Optics Express
·
2021
·Vol. 12
·期 6
·3643-3657
·
IF 3.732
Wenzhao Yang,Wenzhao Yang,Wei Wang,Wei Wang,Lili Jing,Lili Jing,Sung-Liang Chen,Sung-Liang Chen,Sung-Liang Chen,Sung-Liang Chen
The zebrafish has emerged as a useful model for human hematological disorders. Transgenic zebrafish that express green fluorescence protein (GFP) in red blood cells (RBCs) visualized by fluorescence microscopy (FLM) is a fundamental approach in such studies to understand the cellular processes and biological functions. However, additional and cumbersome efforts are required to breed a transgenic zebrafish line with reliable GFP expression. Further, the yolk autofluorescence and finite GFP fluorescence lifetimes also have an adverse impact on the observation of target signals. Here, we investigate the identification of intracerebral hemorrhage (ICH) and hemolytic anemia (HA) in zebrafish embryos using label-free photoacoustic microscopy (PAM) for imaging. First, ICH and HA in transgenic LCR-EGFP zebrafish are mainly studied by PAM and FLM. The results show that PAM is comparable to FLM in good identification of ICH and HA. Besides, PAM is more advantageous in circumventing the issue of autofluorescence. Secondly, ICH and HA in the transparent casper zebrafish without fluorescent labeling are imaged by PAM and bright-field microscopy (BFM). Because of the high contrast to reveal RBCs, PAM obviously outperforms BFM in the identification of both ICH and HA. Note that FLM cannot observe casper zebrafish due to its lack of fluorescent labeling. Our work proves that PAM can be a useful tool to study blood disorders in zebrafish, which has advantages: (i) Reliable results enabled by intrinsic absorption of RBCs; (ii) wide applicability to zebrafish strains (no requirement of a transgene); (iii) high sensitivity in identification of ICH and HA compared with BFM.
研究领域:
医学影像学
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血液学
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发育生物学
✓ 已验证
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2016
·
IF 3.42
Jing Li,Yingyu Guo
Herein, the present work devoted to study the basic capacity of nanoporous silica nanoparticles in being drug carrier that covered structure, wettability and hemolysis so as to provide crucial evaluation. Typical nanoporous silica nanoparticles that consist of nanoporous silica nanoparticles (NSN), amino modified nanoporous silica nanoparticles (amino-NSN), carboxyl modified nanoporous silica nanoparticles (carboxyl-NSN) and hierachical nanoporous silica nanoparticles (hierachical-NSN) were studied. The results showed that their wettability and hemolysis were closely related to structure and surface modification. Basically, wettability became stronger as the amount of OH on the surface of NSN was higher. Both large nanopores and surface modification can reduce the wettability of NSN. Furthermore, NSN series were safe to be used when they circulated into the blood in low concentration, while if high concentration can not be avoided during administration, high porosity or amino modification of NSN were safer to be considered. It is believed that the basic evaluation of NSN can make contribution in providing scientific instruction for designing drug loaded NSN systems.
研究领域:
材料科学
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生物医学工程
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药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-12
·
IF 5.3
Chen Yu,Li Ruiwen,Shen Hao,Li Nana,Gao Wenjing,Guo Haipeng,Feng Bin,Yu Shaoning
As one of the most harmful pathogenic bacteria in shrimp aquaculture, Vibrio parahaemolyticus often causes massive mortality in shrimp. Accurate and rapid detection of V. parahaemolyticus in shrimp farming is essential for avoiding huge economic losses caused by related diseases. In this study, we designed a dual-recognition platform for efficient identification and quantification of V. parahaemolyticus . First, the target bacterium was captured with magnetic beads functionalized by aptamers (Apt-MBs), and then, the broad-spectrum fluorescent probe FcMBL@CdSe-ZnS was used to detect the bacterium based on the interactions between fragment crystallizable mannose-binding lectin (FcMBL) and pathogenic bacteria. The proposed dual-recognition strategy centered around aptamers and FcMBL@CdSe-ZnS was applied to definite quantification of V. parahaemolyticus over a wide range of 10–10 8 CFU/mL with a limit of detection of 4 CFU/mL within 55 min. The feasibility was demonstrated by using the platform to detect V. parahaemolyticus from shrimp intestine, aquaculture water, and seawater. Graphical
研究领域:
微生物学
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水产养殖
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Wei Zhang,Qiao Tang,Mengmeng Wang,Pei Liang,Xuejiao Zhang,Kun Pang,Qiang Chen,Xiaoping Yu
In this work, an aptamer sensor (aptasensor) for SERS detection of Vibrio parahaemolyticus was prepared by utilizing flower-shaped silver nanoparticles coated with graphene oxide (Ag@GO) and core–shell Au@Ag nanoparticles embedded with the Raman reporter 4-mercaptobenzoic acid (4MBA). Graphene oxide (GO) was synthesized using an improved Hummers method and wrapped around thiol-functionalized Ag nanoflowers to form the Ag@GO composite. The SERS aptamer sensor with the strongest Raman signal of 4MBA was developed by combining Ag@GO with Au@4MBA@Ag NPs modified by aptamer through hydrogen bonding and π-π stacking interactions. The aptasensor exhibited good repeatability and stability, and demonstrated high specificity. And it showed a good linear relationship for Vibrio parahaemolyticus detection within the concentration range of 10 2 cfu/mL–10 7 cfu/mL. Introducing GO as an internal standard improved the linear regression coefficient (R 2 ) of detection from 0.9885 to 0.9956. Additionally, spiked real samples of fresh shrimp were tested, yielding recovery rates between 94.1 % and 104.7 %, which demonstrate the feasibility and practicality of this method.
研究领域:
食品安全检测
·
纳米技术
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2016
·
IF 3.388
Can Xu,Jinglei Li,Liuqing Yang,Fang Shi,Liu Yang,Ming Ye
This study aimed to investigate the antibacterial activity of Lachnum YM30 intracellular melanin (LIM). The dilution method was used to measure the MIC and MBC of Gram-negative bacteria Escherichia coli, Salmonella typhi , Vibrio parahaemolyticus and Gram-positive bacteria Listeria monocytogenes , Bacillus megaterium , Staphylococcus aureus . MIC values for each bacterial strain were 0.4, 2.4, 0.2, 0.6, 0.8, 0.3 mg/mL, and the MBC values were 0.4, 3.2, 0.3, 0.8, 0.8, 0.4 mg/mL, respectively. Bacteriostasis of LIM against S. aureus (G + ) and V. parahaemolyticus (G − ) was further revealed by SEM observation, testing integrity of cell membrane, NPN uptake and membrane potential. MTT experiment was used to estimate the cell cytotoxicity of LIM. The results showed that LIM significantly damaged the integrity of cell membrane, increased the leakage of cell contents, raised the quantity of NPN uptake and reduced the membrane potential. LIM had no obvious cell cytotoxicity at low concentrations. These findings indicated that LIM had antibacterial activity and could be applied as a potential natural bacteriostatic agent in the food or pharmaceutical industry.
研究领域:
微生物学
·
食品科学
·
药理学
✓ 已验证
DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Hongyong Lin,Tengyang Liao,Yaoyao Sang,Jin Mao,Yu Liu,Bin Zhang,Di Sun,Wei Jiang
Highly accurate and sensitive detection of Vibrio parahaemolyticus is crucial for ensuring seafood safety and preventing the transmission of foodborne diseases. Herein, a dual-recognition strategy-driven colorimetric biosensor for the detection of V. parahaemolyticus was developed. This biosensor was based on the use of PEG-mediated cefepime-functionalized magnetic nanoparticles (Cefe-PEG-MNPs) and aptamer-modified Fe-doped polydopamine (Fe@PDA) nanozymes. The magnetic platform Cefe-PEG-MNPs was introduced to enhance the enrichment efficiency of V. parahaemolyticus , achieving a capture efficiency exceeding 90%. The Fe@PDA nanozyme exhibited notable peroxidase mimetic enzyme activity. The colorimetric biosensor developed for V. parahaemolyticus detection demonstrated good sensitivity, with a detection range spanning from 2.1 × 10 1 to 2.1 × 10 6 CFU/mL, and a limit of detection of 21 CFU/mL. Moreover, the biosensor demonstrated remarkable specificity, achieving good recovery rates from 95.80% to 103.80% in Penaeus vannamei with a relative standard deviation of less than 1.38%. The newly developed colorimetric biosensor offers a remarkable opportunity for the accurate and sensitive detection of V . parahaemolyticus in seafood.
研究领域:
食品安全
·
微生物检测
·
纳米技术
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Qingqing Zhang,Tingting Zhai,Yuxin Guo,Yifan Weng,Nan Zhou,Han Lin,Hao Tan,Kangning Lu,Yiyi Zhou
Highly selective and sensitive detection of foodborne pathogens is crucial for ensuring food safety. In this work, a photoelectrochemical (PEC) aptasensing platform was developed for the selective and sensitive detection of Vibrio parahaemolyticus (VP), adopting Faraday cage-type construction mode. When VP was present in the sample, the aptamer for VP (Apt) assembled on the Apt/Bi 2 S 3 /ITO electrode captured VP, which bound GO@Cu 2 O-Apt to the electrode surface. The graphene oxide (GO) in GO@Cu 2 O-Apt has a large surface area and good conductivity, on the surface of which a large amount of Cu 2 O that can switch the polarity of the Bi 2 S 3 photocurrent was loaded, resulting in a highly selective and sensitive detection of VP with a detection limit of 1.0 CFU/mL. By adjusting the Apt, the platform can be used for the detection of other foodborne pathogens, offering broad application potential in foodborne pathogen detection.
研究领域:
食品安全检测
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Hai Jiang,Zhikang Sun,Qian Guo,Xuan Weng
Vibrio parahaemolyticus is one of the foodborne bacteria that widely present in seafood as well as the leading cause of seafood-associated bacterial gastroenteritis. Traditional identification of such pathogens mainly relies on culturing methods, ELISA or PCR. These methods are usually laborious, time-consuming with poor diagnosis competences, or require costly and bulky equipment though of high sensitivity. In this study, a thread-based microfluidic electrochemical aptasensor was designed, fabricated and tested by using label-free aptamer immunosensing technology for rapid and highly sensitive detection of Vibrio parahaemolyticus in seafood. Both the microfluidic channels and electrodes were simply fabricated on threads. Molybdenum disulfide (MoS 2 ) nanosheets were used to obtain enhanced sensitivity of the electrochemical measurement. When used in detecting Vibrio parahaemolyticus , the proposed aptasensor has a dynamic detection range of 10–10 6 CFU mL −1 with a detection limit of 5.74 CFU mL −1 . Compared with traditional plate counting method, the proposed aptasensor has higher detection sensitivity and less assay time (30 min), while high specificity and accuracy are kept. The proposed microfluidic thread-based electrochemical aptasensor grabs the potential to detect other pathogens by simply functionalizing the threaded electrodes with aptamers for targeted biological substances.
研究领域:
食品安全
·
生物传感技术
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 22
·3537
·
IF 4.7
Siyang Chen,Wenxiu Zhu,Yiqun Zhan,Xiaodong Xia
Although phloretin is widely utilized in the food industry as an additive, its effects on foodborne pathogens remain insufficiently investigated. This study aimed to evaluate the antimicrobial properties of phloretin (PHL) againstVibrio parahaemolyticus(V. parahaemolyticus) and to elucidate the potential mechanisms of action. After PHL treatment, alterations in the cell morphology, cell microstructure, and intracellular contents ofV. parahaemolyticuswere assessed. Scanning electron microscopy revealed substantial damage to cell integrity, subsequent to PHL treatment. A notable reduction in intracellular components, including proteins, ATP, and DNA, was observed in samples treated with PHL. PHL was shown to inhibit the activities ofATPase, β-galactosidase, and respiratory chain dehydrogenase inV. parahaemolyticus. Furthermore, it was demonstrated to elevate the intracellular levels of reactive oxygen species and promote cell death. After being applied to sea bass, shrimp, and oysters, PHL effectively inactivatedV. parahaemolyticusin these seafoods. These findings demonstrate that PHL has potential for application in seafood to controlV. parahaemolyticus.
研究领域:
食品科学
·
微生物学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Yuhao Zhang,Luting Yan,Lei Tian,Qi Guo,Maosheng Sang,Shouyi Dou,Falan Li,Qingqing Yang,Xia Sun,Yemin Guo,Yanyan Zhang
Vibrio parahaemolyticus ( VP ) is one of the most common pathogens in aquatic products, and its study using electrochemiluminescence is still relatively rare. In this study, an electrochemiluminescent (ECL) biosensor was developed to detect VP in aquatic products using AuNPs modified PEI/MWCNTs (AuNPs@PEI/MWCNTs) as a substrate material and ordered mesoporous carbon (OMC) adsorbed Ru(bpy) 3 2+ (OMC@Ru(bpy) 3 2+ ) as a luminescent material. To increase the conductivity of the biosensor and offer a favorable loading environment for the immobilization of the luminescent probe, AuNPs@PEI/MWCNTs are employed as the substrate. Meanwhile, the OMC@Ru(bpy) 3 2+ functionalized DNA probe acts as a luminescent and capture probe to generate ECL signals and specifically recognize different concentrations of VP . The biosensor has good selectivity and stability and has been successfully applied to the detection of VP in scallops. This method not only expands the potential uses of bioprobe-based ECL biosensors, but also offers a new and effective approach used to detect food-borne pathogens in aquatic products.
研究领域:
食品科学
·
生物技术
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）