NEW JOURNAL OF CHEMISTRY
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2025
·
IF 2.5
Zhanghong Guo,Lin Zhou,Haining Cui,Jin-Xin Ma,Chan Wang,Qijun Song
Natural laccase, a member of the copper blue oxidase family, has its catalytic sites are classified as type1 (T1), type2 (T2) and type3 (T3) Cu. The electron transfer distance between the T1 to T2/T3 Cu has a great effect on the electron transfer efficiency in the laccase-catalyzed reaction. To mimic the active sites of laccase, a copper-doped carbon dots (CuCDs) was synthesized by a one-step hydrothermal treatment of copper sulfate and riboflavin. Experiment characterizations and theoretical simulations revealed that the catalytic sites in CuCDs consist of N, O coordinated copper and quinoxaline structure, which resemble the coordination environment of T2 Cu and the function of the T3 Cu center, respectively. The short electron transfer distance of 4.8 Å in catalytic sites of CuCDs accelerates the oxidation of the substrate dopamine (DA) to polydopamine (PDA) with a reaction rate being 40 times faster than that of laccase. Galvanic cell experiments proved that electron transfer from DA to oxygen was achieved in the presence of CuCDs. Therefore, this study not only could provide an efficient and eco-friendly nanozyme for hair dyeing in ambient condition, but also could inspire more and deeper researches in the field of mimicking natural enzyme with carbon nanomaterials.
研究领域:
生物化学
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纳米材料科学
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催化反应研究
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产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Xiaomin Zhu,Youren Duan,Jianqi Lu,Wei Xia,Yujia Peng,Jiawei Liu,Weiliang Dong,Min Jiang
Polyurethane (PU), a segmented block copolymer with chemically resistant urethane linkages and tunable architecture, presents persistent biological recycling challenges. This study presents a Bacterial Laccase-Mediated System (BLMS) derived from Bacillus subtilis for efficient degradation of polyester- and polyether-PU. Utilizing the laccase CotA and mediator 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the BLMS demonstrated effective de polymerization of both commercial and self-synthesized PU foams, including polyester- and polyether-types. The weight loss of the self-synthesized polyester-foam and the commercial polyether-foam reached up to 21.24 ± 1.20% and 3.81 ± 0.36%, respectively. Subsequently, we detected oxygenated products such as ketones, alcohols, aldehydes, acids, esters, ethers, and 2,4– toluenediamine (2,4-TDA) indicating that bacterial laccase CotA exhibited redox catalytic activity toward PU. Moreover, an interesting phenomenon was observed during the degradation process that the solution turned purple. We predicted that this attribute to the enzymatic oxidation of ABTS to the radical cation ABTS·+, which subsequently reacts with 2,4-TDA to form the purple product. This study finds a plastic degrading enzyme capable of hydrolyzing urethane bonds in PU, offering a promising contribution to the development of a bio-based circular economy for PU biodegradation and recycling.
研究领域:
生物降解
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酶催化
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高分子科学
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生物工程
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环境生物技术
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产品关联
DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2024
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IF 6.7
Zhao Li,Kanghui Ning,Ning Guo,Yuanyuan Shao,Song Li,Daoji Wu,Xiangzheng Ren,Lin Wang
Tetracycline (TC) antibiotics have been widely used in aquaculture and medical industries owing to their low cost, high efficiency, and broad-spectrum characteristics. However, it is difficult to degrade naturally in the environment, leading to its large accumulation in wastewater. The enzymatic degradation method using laccase exhibits good application potential in TC removal. In this study, low-cost activated carbon (AC) was used as the carrier to immobilize laccase using the adsorption–cross-linking method, forming Laccase@AC, characterized by high efficiency, eco-friendliness, and cost-effectiveness. Laccase@AC was used to treat TC-contaminated water. Its stabilities and catalytic activity for TC were comprehensively investigated. The results showed that Laccase@AC exhibited high enzyme loading capacity (407.97 mg/g), activity retention (1.36 U/mg), and stabilities. Moreover, Laccase@AC exhibited a higher TC removal efficiency than free laccase. The removal rates of 20 mg/L of TC using Laccase@AC were 78.7 % and 99 % after 6 h and 24 h, respectively, in the presence of a mediator (syringaldehyde, SA), whereas only 14.9 % of TC was removed using the same amount of free laccase. Temperature and TC concentration affected the performance of Laccase@AC. The TC optimal removal rate was achieved at 40–60℃ using 1 mg of Laccase@AC per 15 mg TC. Degradation product analysis revealed that catalytic oxidation was the main reaction catalyzed by laccase and enhanced by SA. The AC provided a stable reaction substrate and enhanced adsorption capacity. Meanwhile, the biocatalytic system effectively degraded TC and produced downstream products with low toxicity, demonstrating its potential for treating antibiotic wastewater using immobilized laccase. Therefore, immobilized laccase is an efficient biocatalyzer for eliminating TC in wastewater, and it has a broad application prospect in the treatment of antibiotic-contaminated wastewater.
研究领域:
环境科学
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生物技术
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产品关联
DOI
高影响因子
Materials
·
2020
·Vol. 13
·期 2
·437
·
IF 3.057
Heming Song,Hongge Jia,Qingji Wang,Xinyi Zhao,Guoxing Yang,Mingyu Zhang,Hailiang Zhou,Shuangping Xu,Yu Zang,Yazhen Wang,Liqun Ma
Corn straw is an agricultural waste. The system for extracting cellulose from corn straw at a high temperature has been widely reported by researchers. However, the system for extracting cellulose from corn straw at a low temperature has been rarely reported. In this paper, a new system for extracting cellulose from corn straw at a low temperature was reported for the first time. This new system is designated as the low temperature laccase system (LTLS). Cellulose was successfully extracted from corn straw by the LTLS, and the used solution could be recycled. Therefore, the low temperature laccase system is an environmentally-friendly system. The cellulose content in corn straw is 30–40%. The yield of cellulose extracted by LTLS was 33%. The obtained cellulose product was creamy white. The extracted cellulose samples were characterized by using infrared spectroscopy (IR), thermogravimetry (TG), and X-ray diffraction (XRD). The results were consistent with that of standard cellulose. We confirmed that the LTLS extracted cellulose from corn straw with high purity.Keywords:cellulose;corn straw;low temperature;laccase
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2021
·
IF 13.273
Zhihui Chen,Jun Yao,Tatjana Šolević Knudsen,Bo Ma,Bang Liu,Hao Li,Xiaozhe Zhu,Chenchen Zhao,Wancheng Pang,Ying Cao
The environmental impact of the mining industry requires efficient and eco-friendly technologies to mitigate the presence of mineral flotation reagents (MFRs) in mineral processing wastewater (MPW) prior to their discharge into the environment. In this work, for the first time, a robust, easily separable and reusable biocatalyst, Fe 3 O 4 @SiO 2 -NH 2 -Lac, was used for the degradation of a novel mineral flotation reagent 8-hydroxyquinoline (8-HQ). Under optimized conditions, Fe 3 O 4 @SiO 2 -NH 2 -Lac achieved 89.2% 8-HQ degradation efficiency within 6 h. The effect of the main constituents of MPW on 8-HQ degradation, including metal ions, organic solvents, surfactant, metal chelator and flotation frother was evaluated. The Fe 3 O 4 @SiO 2 -NH 2 -Lac also displayed favorable degradation efficiency of 8-HQ in real lead–zinc mine water. The biocatalyst could be easily recovered and had a satisfactory reusability, retaining 64.5% of 8-HQ degradation efficiency in the sixth reaction cycle. Identification of intermediate products revealed that Fe 3 O 4 @SiO 2 -NH 2 -Lac mediated reaction predominantly generated various structural 8-HQ oligomers/polymers. A potential degradation pathway for 8-HQ was speculated as follows: Fe 3 O 4 @SiO 2 -NH 2 -Lac initially catalyzed the oxidation of 8-HQ to yield the corresponding reactive radical intermediates, which subsequently undergone self-coupling reaction via C − C and C − O − C covalent coupling at their ortho and/or para positions, finally forming oligomers and polymers. The inhibition assays of marine bacterium ( Vibrio fischeri ) demonstrated that the toxicity of 8-HQ and its intermediate products was effectively reduced after Fe 3 O 4 @SiO 2 -NH 2 -Lac treatment. The results of this study might present an alternative immobilized laccase-based clean biotechnology for the clean-up and detoxification of 8-HQ contaminated MPW.
研究领域:
环境科学
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生物技术
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化学工程
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材料科学
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产品关联
DOI
高影响因子
Waste and Biomass Valorization
·
2018
·Vol. 10
·期 11
·3343-3350
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IF 1.874
Chen Cheng,Jiang Linfeng,Ma Guanfeng,Jin Dongxue,Zhao Lisha,Ouyang Xinping
Abstract Lignin removal from lignocellulosic materials plays a great role in the utilization cellulose with high efficiency. In order to improve the quality of reconstituted tobacco sheet, lignin existed in tobacco stem was removed by enzymatic hydrolysis with laccase enhanced by weak alkali treatment together with the addition of Tween 80 into laccase treatment system. It is found that lignin was partially removed by laccase. The treatment of tobacco stem with NaHCO 3 resulted in the dissolution of lignin, and hence increased the porosity and specific surface area of the pretreated tobacco stem. Consequently, laccase could access to the tobacco stem more completely. The surface activity and dispersion of Tween 80 improved laccase activity, solubilization of degraded products of lignin, and contact interface between laccase and tobacco stem. The synergistic action of laccase, weak alkali and Tween 80 improved the ratio of lignin removal significantly, reaching as high as 40.3%. Graphical Abstract
研究领域:
生物化学
·
植物学
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2018
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IF 2.932
Fan Li,Yuanyuan Yu,Qiang Wang,Jiugang Yuan,Ping Wang,Xuerong Fan
Polydopamine (PDA) films are generally produced by spontaneous oxidation of dopamine in basic medium, which is a highly convenient method for surface modification. This method is, however, not suitable for alkaline sensitive materials, which have poor stability in alkali medium and need over-long reaction time. Herein, we compared two dopamine polymerization methods: conventional method (induced by O 2 at pH 8.5) and laccase-catalyzed method (at pH 5.5). The comparison showed that the polymerization of dopamine was greatly improved when catalyzed by laccase. The PDA film obtained from laccase-catalyzed method was more uniform and more stable compared with that obtained from the conventional method; the film was able to further react with amine-terminated molecules. As a result of laccase, ether bonds were found between dopamine units of PDA; while such bonds were not observed in film obtained from the conventional method. This work can be a basis for a better understanding of structure and property relationships of PDA coating.
研究领域:
生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 6
·920
·
IF 2.861
Youxun Liu,Yuanyuan Geng,Mingyang Yan,Juan Huang
The successful encapsulation of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), a well-known laccase mediator, within a mesoporous metal-organic framework sample (i.e., MIL-100(Fe)) was achieved using a one-pot hydrothermal synthetic method. The as-prepared ABTS@MIL-100(Fe) was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, nitrogen sorption, and cyclic voltammetry (CV). Our ABTS@MIL-100(Fe)-based electrode exhibited an excellent electrochemical response, indicating that MIL-100(Fe) provides an appropriate microenvironment for the immobilization and electroactivity of ABTS molecules. ABTS@MIL-100(Fe) was then evaluated as an immobilized laccase mediator for dye removal using indigo carmine (IC) as a model dye. Through the application of laccase in combination with a free (ABTS) or immobilized (ABTS@MIL-100(Fe)) mediator, decolorization yields of 95% and 94%, respectively, were obtained for IC after 50 min. In addition, following seven reuse cycles of ABTS@MIL-100(Fe) for dye treatment, a decolorization yield of 74% was obtained. Dye decolorization occurred through the breakdown of the chromophoric group by the Laccase/ABTS@MIL-100(Fe) system, and a catalytic mechanism was proposed. We therefore expect that the stability, reusability, and validity of ABTS@MIL-100(Fe) as a laccase mediator potentially render it a promising tool for dye removal, in addition to reducing the high running costs and potential toxicity associated with synthetic mediators.Keywords:laccase;ABTS;metal-organic framework;mediator;decolorization
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Catalysts
·
2024
·Vol. 14
·期 8
·473
·
IF 3.8
Hong Zhang,Zhi Wang,Fengxi Li,Lei Wang,Bo Ren
A stable and efficient biocatalyst was prepared by encapsulatingTrametes versicolorlaccase using an acrylic acid-grafted β-cyclodextrin hydrogel (Lac-CD-PAA). Scanning electron microscopy and nitrogen adsorption-desorption experiments showed that there were regularly distributed channels in the spongy Lac-CD-PAA. In addition, a large number of mesopores and macropores existed in the wall of the hydrogel lamellae. This network structure reduced the diffusion resistance of the hydrogel to the target substrate. The relative activity of the resulting Lac-CD-PAA could be maintained at 35.8% after six cycles of use. Lac-CD-PAA exhibited higher thermal and chemical stability compared to free laccase. The negative charge on the surface of Lac-CD-PAA gives it the ability to pretreat cationic dyes. In six consecutive methylene blue decolorization tests, Lac-CD-PAA decolorized better than free laccase. The results showed that the prepared β-cyclodextrin-based composite hydrogel was a good carrier for laccase.
研究领域:
生物化学
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环境科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Jiahong Lin,Qiujie Lai,Yingju Liu,Shi Chen,Xueyi Le,Xiaohua Zhou
Magnetically retrievable formulations of laccase potentially promising for biomedical and environmental applications were constructed by immobilization of the enzyme on the surface of magnetic nanoparticles functionalized by siloxane layers with active amino and methacrylyol moieties. The functional magnetic particles were prepared using a hydrolytic polycondensation reaction of tetraethoxysilane with 3-methacryloxypropyltrimethoxysilane (MPS). The results indicated that the amounts of laccase loading on the methacrylyol functional magnetic particles were approximately 150 mg/g under the optimum conditions. The stability of the immobilized laccase was highly enhanced against thermal treatments and provided better long-term storage. Compared to free laccase, the immobilized laccase exhibited significantly higher decolourisation efficiency in the decolorization of a representative dye, methyl red, which resulted from the magnetic particles adsorption and enzymatic catalysis. These results show its great potential for industrial applications.
研究领域:
生物技术
·
环境科学
·
医学工程
✓ 已验证
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2025
·
IF 7.3
Jianfeng Zhu,Junjie Huang,Jianhong Yang,Jun Cai,Guoxiang Li
Lacquer sap is a precious natural emulsion collected from lac tree, mainly used as a coating. In order to improve its performance and reduce its usage cost, styrene was mixed into raw lacquer sap for modification. Under an oxygen atmosphere, the copolymerization of styrene and urushiol was achieved through the catalysis of laccase contained in the raw lacquer sap. The copolymerization process was characterized by GPC and NMR. To maintain the fluidity of the copolymerized lacquer, styrene couldn't be completely polymerized. Excessive reaction time could lead to explosive polymerization. Thus, controlling reaction time was particularly important. The copolymerized lacquer had a shorter drying time than the raw lacquer sap. The results from NMR, FT-IR, pyrolysis GC–MS and UV–Vis spectra showed that styrene had successfully polymerized into the dry lacquer film. Due to the introduction of styrene, the color of the lacquer film changed from dark brown to light yellow, and its pencil hardness and flexibility were improved, reaching 5H and 2 mm, respectively, while maintaining its excellent resistance to organic solvents. In addition, the thermal stability of the lacquer film was improved as well.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Environmental Technology & Innovation
·
2020
·
IF 3.356
Xiaochu Chen,Qizhen Zhou,Fengmao Liu,Qingrong Peng,Yanli Bian
The control and removal of pesticide residues from possible pollution source by using improved methods without secondary pollution is essential for reducing its potential hazards to humans. In this work, a method of combining adsorption and degradation for the removal of pesticide residue was investigated based on the biodegradable microporous starch immobilized laccase (LA–MPS). Biosorbent of microporous starch (MPS) were prepared from maize starch by treating with glucoamylase and α -amylase to increase the specific surface area. Then, laccase with natural biological activity was immobilized on the surface of MPS, the prepared LA-MSP was characterized by SEM, FT-IR, activity assay and stability test. Moreover, LA–MPS coupled with mediators were applied to remove two typical triazine herbicides, atrazine and prometryn, in paddy field water sample. The physical action force between the LA–MPS and pesticide molecules resulted in the good adsorption of LA–MPS to the two pesticides. The apparent rate constants of laccase interaction with pesticide molecules were greatly improved by the adsorption process. Compared with natural degradation, the removal efficiency of atrazine and prometryn were increased by 61% in 7 days, which contributed by 43 ∼ 52% of degradation and 10 ∼ 38% of adsorption. These results indicated that the application of LA–MPS prepared by MPS sorbent was a feasible and green approach for the removal of pesticide residues in paddy field water sample.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）