Foods
·
2022
·Vol. 11
·期 5
·671
·
IF 5.561
Andi Wu,Jimin Lv,Changxin Ju,Yiwen Wang,Yanyun Zhu,Jianchu Chen
In this study, a novel method to clarify bayberry juice with composite clarifiers, chitosan and sodium alginate, has been designed. The optimal conditions were as follows: using chitosan 0.05 g/L first and then sodium alginate 0.05 g/L as composite clarifiers, standing for 2 h at 25 °C. The transmittance increased from 0.08 to 91.2% after treating by composite clarifiers, which was significantly higher than using chitosan (44.29%) and sodium alginate (38.46%) alone. It was also found that sedimentation time of juice treated by composite clarifiers was about 60% shorter than using single clarifiers. Meanwhile, the reduction of anthocyanin in juice was 9.16% for composite clarifiers treatment, being less than that for the single sodium alginate and previous related researches. In addition, the color and aroma of bayberry juice treated by composite clarifiers were improved. Juice treated by composite clarifiers had the highestL* value with 52.48 and looked more attractive. The present research revealed that content of beta-damascenone and dihydro-5-pentyl-2(3H)-furanone increased after treatment with composite clarifiers which contributed more to the pleasant aroma. Overall, the developed method improved the clarification effect and sensory quality, and reduced the sedimentation time, which may be promising in the production of clear bayberry juice.Keywords:bayberry juice;clarification;chitosan/sodium alginate;composite clarifiers;aroma
研究领域:
食品科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Ziyu Deng,Fang Wang,Bin Zhou,Jing Li,Bin Li,Hongshan Liang
In this study, polygalacturonase (PG) was immobilized in calcium alginate microspheres prepared using endogenous emulsification method and its application in apple juice clarification was evaluated. Preparation conditions of calcium alginate microspheres prepared by endogenous emulsification were optimized, additionally, the formed microspheres were characterized. Under optimal conditions, microspheres with the particle size of 38.0 ± 0.2 μm showed good sphericity, storage stability and a narrow particle size distribution. Meanwhile, single factor experiment and response surface experiment were combined to get the optimum conditions of immobilizing PG. The immobilized enzyme presented the optimum temperature at 60.0 °C, which was almost 10.0 °C higher than the free PG and the activity was less dependent upon pH within the interval of 3.0–4.5 than free enzyme, supporting the immobilized enzyme more stable in apple juice clarification (pH = 4.3 ± 0.1). The immobilized enzyme also exhibited good operational stability and 63.0% of its initial activity retained after three batch reactions. In the present of the immobilized PG, the turbidity of apple juice decreased, as well as apple juice had good thermal and freezing ability.
研究领域:
食品科学
·
生物技术
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产品关联
DOI
高影响因子
Frontiers in Neuroanatomy
·
2015
·
IF 3.544
Hou Bing,Zhang Dan,Zhao Shan,Wei Mengping,Yang Zaifu,Wang Shaoxia,Wang Jiarui,Zhang Xin,Liu Bing,Fan Lingzhong,Li Yang,Qiu Zilong,Zhang Chen,Jiang Tianzi
Efficient optical clearance is fundamental for whole brain imaging. In particular, clearance of the brain without membrane damage is required for the imaging of lipophilic tracer-labeled neural tracts. Relying on an ascending gradient of fructose solutions, SeeDB can achieve sufficient transparency of the mouse brain while ensuring that the plasma membrane remains intact. However, it is challenging to extend this method to larger mammalian brains due to the extremely high viscosity of the saturated fructose solution. Here we report a SeeDB-derived optical clearing method, termed FRUIT, which utilizes a cocktail of fructose and urea. As demonstrated in the adult mouse brain, combination of these two highly water-soluble clearing agents exerts a synergistic effect on clearance. More importantly, the final FRUIT solution has low viscosity so as to produce transparency of the whole adult rabbit brain via arterial perfusion, which is impossible to achieve with a saturated fructose solution. In addition to good compatibility with enhanced yellow fluorescent protein, the cocktail also preserves the fluorescence of the lipophilic tracer DiI. This work provides a volume-independent optical clearing method which retains the advantages of SeeDB, particularly compatibility with lipophilic tracers.
研究领域:
神经科学
·
生物医学工程
·
光学成像
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Xiaorong Song,Zhihui Chai,Yuan Zhu,Chenglin Li,Xinquan Liang
The phenolic substances in sugar cane juice affect the color value of white sugar. A magnetic chitosan-modified-diatomite (MCMD), as a new type of clarifying agent for sugar, prepared via a co-precipitation method combined with cross-linking treatment was firstly used to adsorb two primary phenolic acids of sugarcane juice: gallic acid (GA) and caffeic acid (CA). The maximum adsorption capacities for GA and CA by MCMD were 31.949 mg g –1 and 27.640 mg g –1 , respectively. Additionally, our results showed that adsorption of GA and CA on MCMD fitted well with the pseudo-second-order model and Langmuir equation, respectively. The adsorption, a physical spontaneous endothermic process, might have involved electrostatic attraction and micropore filling. Importantly, MCMD has good regenerability and achieves better sulfur-free clarification of sugar cane juice and improves more significantly the food safety of white sugar than the traditional decolorization method.
研究领域:
食品科学
·
环境工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Jingwen Yang,Xinyu Zhang,Qimeng Lu,Lei Wang,Xueqin Hu,Hongbin Zhang
Polysaccharides have been widely used in many fields. In the field of food water treatment, polysaccharide-based flocculants have attracted extensive attention. Based on the enzymatic preparation of high molecular weight dextran, two safe, sustainable and eco-friendly dextran based high molecular flocculants were developed by introducing flocculating active groups into the molecular chain of dextran through cross-linking (DEPL) and esterification (Dex-PL). The prepared product was characterized by elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis and field emission scanning electron microscopy (FESEM). Because amino group is the main active group of flocculation, more amino groups are retained in esterification reaction. In the flocculation experiment of kaolin suspension, the efficiency of the two flocculants is very excellent, both of which are greater than 96%. The results of the study on the flocculation mechanism showed that charge neutralization, adsorption bridging and sweeping flocculation mechanisms occurred in the flocculation process. Dex-PL was applied to the clarification of sugarcane juice in sugar industry. At the dosage of 118 mg/L, the clarification efficiency reached 91.4%. The results of biodegradation experiment showed that the product can be biodegraded by enzymatic hydrolysis. This study provides experience for the development and application of new polysaccharide based flocculants, and provides safe and effective flocculants for sugar refining.
研究领域:
食品科学
·
环境工程
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Qiangjian Yang,Jianquan Luo,Shiwei Guo,Xiaofeng Hang,Xiangrong Chen,Yinhua Wan
Clarification operations are vital to extract high-quality products from cane molasses. Ultrafiltration (UF) is very efficient for this purpose, although membrane fouling limits its large-scale application. In this work, five commercially available polyethersulfone UF membranes were evaluated for the clarification of molasses, whereby 30–100 kDa membranes were found to be the most suitable for this purpose. In order to minimise fouling formation during molasses clarification, the threshold flux concept was for the first time applied to dilution–concentration membrane processes. The threshold flux in concentration mode could be determined by correlating the average transmembrane pressure, average fouling rate, and specific energy consumption with the permeate flux at the same feed. An optimal dilution factor of eight was found based on such threshold flux concept and, only above the threshold flux, higher retentate concentrations and longer concentration times resulted in obvious fouling aggravation. Moreover, polydopamine coating and subsequent polymer (polyethyleneimine, chitosan oligosaccharide , alginate dialdehyde) grafting were used to tune the membrane surface properties to further control the membrane fouling. It was found that membranes modified with positive charges were more prone to molasses fouling, where the effect of the membrane surface properties on fouling was significant only above the threshold flux. However, pore narrowing after surface modification may increase the foulant retention and accumulation on the membrane, thus exerting certain influence on the threshold flux. The threshold flux value for the clarification of molasses with 50 and 100 kDa UF membranes was found to be 27–32 L m −2 h −1 . The outcome of this work not only provides a guide to study threshold fluxes for industrial fluids at high concentrations, but also clarifies the effect of surface modification on the concentration polarisation/fouling formation during the clarification of cane molasses.
研究领域:
化学工程
·
环境工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Ke Yang,Xiang Yang,Xiang Zhao,Marc Lamy de la Chapelle,Weiling Fu
Simple, rapid, and efficient cell viability assays play a fundamental role in much of biomedical research, including cell toxicology investigations and antitumor drug screening. Here, we demonstrate for the first time a rapid and label-free cell viability assay using THz spectroscopy in combination with a new optical clearing agent (OCA) and microfluidic technology. This strategy uses a considerably less absorptive OCA to replace the highly absorptive water molecules around the living cells and thus to decrease the background signal interference. Three low-viscosity oils were screened as potential OCA candidates, among which fluorinated oil was selected because of its lower absorption and lowest cytotoxicity. After the liquid medium was replaced with fluorinated oil in a microfluidic chip, an obvious THz spectral difference was observed between the fluorinated oils with and without living cells. This change in THz response was preliminarily attributed to the distinguishable signals between the cells and the fluorinated oil. In addition, we applied this method to cell viability assays of human breast cancer cells (MDA-MB-231) after treatment with different antitumor drugs. The results indicated that THz spectroscopy with the aid of the proposed water-replacement strategy presented excellent quantification of cell viability with the advantages of a rapid, label-free, nondestructive microassay, which offers significant potential to developing a convenient and practical cell analysis platform.
研究领域:
生物医学工程
·
细胞生物学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Richard Ansah Herman,Xuan Zhu,Ellen Ayepa,Marriam Khurshid,Zhan-Peng Zhang,Shuai You,Jun-Feng Qian,Jun Wang
The juice processing industry offers several benefits, including promoting health and wellness through the delivery of beverages rich in nutrients. Nonetheless, the industry encounters significant challenges regarding new technologies for processing and preservation given that they can be costly and labor-intensive. In this study, magnetic Janus silica (SiO 2 ) nanoparticle which offers multifunctionality and high stability was synthesized and subsequently immobilized with a protease mutant T70I (T70I@MSNs) to serve as a clarifying agent in juice. The optimal conditions for the protease immobilized with SiO 2 nanoparticles showed superior tolerance and stability at pH 5 and 65 °C. Box-Behnken Design revealed optimizing conditions of 60 min, 50 °C and 0.2 % enzyme concentration which significantly influenced the clarification of the juice samples. Immobilized T70I@MSNs decreased >70 % of juice turbidity while maintaining about 78 % of enzyme activity after 7 consecutive cycles of reuse. Notably, T70I@MSN treatment enhanced certain volatile components by increasing alcohol and ester production, while changes in surface microstructure and functional groups were observed. This study highlights the significant improvement of magnetic Janus SiO 2 nanoparticles immobilized proteases in the clarification of juice against haze and undesirable properties, thereby offering a notable contribution to the juice processing industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2020
·Vol. 44
·期 3
·e13133
·
IF 1.662
Haidong Tan,Guojun Yang,Wei Chen,Qishun Liu,Kuikui Li,Heng Yin
Endo-polygalacturonase II B (PgaB) from Aspergillus luchuensis was orthologous to endo-polygalacturonase from Aspergillus niger with mutant sites Thr42Ser and Glu52Ala. Mature pga B gene was cloned from the genomic DNA of A. luchuensis and secreted expressed with over 90% purity in Pichia Pastoris and reached 1.0 g/L after 144 hr culture. The recombinant PgaB was further purified by Ni-NTA chromatography. Using polygalacturonic acid (PGA) as substrate, the optimal condition for PgaB activity was 40°C and pH 4.5, respectively. Km and Vmax of PgaB were 0.19 mmol/l and 103.58 μmol min −1 mg −1 , respectively. The relative activity of PgaB remained more than 60% and 40% of maximum activity at 50 and 60°C for 7 hr. PgaB increased the light transmittance by 85% and showed high efficiency in juice clarification. The main product was galacturonic acid oligosaccharides with degrees of polymers (DP) 1–3. The PgaB is a potential pectinolytic enzyme in food industries. Practical applications Endo-polygalacturonase II B (PgaB) was identified from Aspergillus luchuensis , a filamentous fungus widely used in food and beverage fermentation in East Asia. PgaB still kept its most activity at 60°C for 7 hr. Polygalacturonic acid (PGA) can be digested effectively by the PgaB and the main products are galacturonic acid oligosaccharides with degrees of polymers (DP) 1–3. PgaB shows high efficiency in juice clarification. The PgaB is a potential pectinolytic enzyme for the applications in food industries.
研究领域:
酶学
·
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
·
2021
·
IF 0.896
Peng Zhu,Limin Wu,Wei Liu,Baozong Li,Yi Li,Yonggang Yang
A series of pyridinium-based chiral ionic liquid crystals were designed and synthesized. All of them exhibit enantiotropic smectic phases. The methylation on the pyridine ring not only decreases the melting point and clearing point, but also drives structural transitions from bilayer molecular packing structures to interdigitated monolayer ones at the smectic A and C phases. Due to entropy increase, the clearing point of the liquid crystal derived from 2-methylpyridine is higher than that derived from 3-methylpyridine, and then that derived from 4-methylpyridine.
研究领域:
材料科学
·
物理化学
·
液晶
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Xiujun Wang,Jian Zhang,Qian Xu,Ming Duan,Shenwen Fang,Chunsheng Zhang,Jiaqing Chen
Nonionic polyether water clarifier is commonly used in the treatment of produced water from offshore oilfield. At the meantime, hydrophobically modified polyacrylamide (HMPAM) is one of the commonly used polymer oil displacement agents. Predicting the influence of HMPAM on the performance of nonionic polyether water clarifier is of great significance for the treatment of produced water. This work explored the influence of different HMPAMs on the hydrophilic-lipophilic deviation (HLD) equation of a typical nonionic polyether water clarifier AE411 based on the HLD theory, and predicted their influences on the performance of AE411. The results showed that HMPAM had no effect on the parameter K (reflecting the interaction between AE411 hydrophilic groups and oil phase) and c t (reflecting the temperature sensitivity of AE411) in HLD equation of AE411, but reduced the parameter C cn (reflecting the micelle structure of AE411 in water) and led to an increase in temperature corresponding to AE411′s HLD = 0. It was predicted that higher temperature was needed to ensure the performance of AE411. The experimental results of produced water treatment confirmed this prediction. The results of the paper validated the reliability of HLD application in the field of produced water treatment and provided some guidance for the development of nonionic polyether water clarifier.
研究领域:
环境科学
·
化学工程
·
石油工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Wenkang Zhang,Qiqi Yang,Qilin Gu,Zheng Liang,Zhijun Xu,Zhaoxiang Zhong,Weihong Xing
Ceramic membranes ultrafiltration (UF) has emerged as a promising alternative to the conventional clarification technology in sugarcane juice clarification industries, given their efficient and eco-friendly separation processes based on the size sieving effect. However, the upscaling application of ceramic UF membranes is still limited by their low permeate flux, high energy consumption and severe membrane fouling. Herein, to utilize the interactions at the interfaces, tubular SiC microfiltration (MF) membranes with special surface wettability were deliberately tailored through a novel penetration pressure counter-balanced crossflow method using 1H, 1H, 2H, 2H-perfluorooctyltrichlorosilane as a hydrophobic agent. The effect of an out-layer pressures (0-4.0 kPa) and flow rate (1.4-2.4 mL·s -1 ) on surface wettability of membranes were studied, and those obtained membranes were applied in sugarcane juice clarification. The wettability, functional groups, binding energy, chemical compositions, and microstructure of modified SiC membranes were analyzed to elucidate their wettability-performance relationships. The performance of modified SiC MF membranes in sugarcane juice clarification were mainly evaluated by the flux decline profiles and permeate solution turbidity. The SiC membrane with hydrophobic inner wall (WCA=142°) and hydrophilic outer surface (WCA=0°) were achieved at out-layer pressure of 2.0 kPa and flow rate of 1.9 mL·s -1 . The results show that the SiC membrane with such asymmetric wettability exhibited ultra-high stable permeate flux (844 L·m -2 ·h -1 ·bar -1 ), satisfactory turbidity reduction rate (95.7%), and relatively low flux decreased rate (64.4%). The improved antifouling performance can be primarily attributed to the hydrophobic inner wall, and negatively charged pore wall. This work primarily demonstrates the application potential of ceramic MF membranes with asymmetric wettability for the effective sugarcane juice clarification.
研究领域:
膜科学
·
化学工程
·
食品加工
·
材料科学
·
环境技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）