Sustainable Chemistry and Pharmacy
·
2025
·
IF 5.8
Mingzhu Guo,Yuewen Shao,Shu Zhang,Xun Hu
Fungi are an important category of biomass resource, which is carbonaceous and could be potential precursors for production of activated carbon (AC). Nonetheless, distinct composition of fungi from woody biomasses might produce AC of different pore characteristics. Herein, two common fungal species, white jade fungus (WJF) and mushrooms, were activated with K 2 C 2 O 4 , ZnCl 2 or H 3 PO 4 . The resulting ACs were analyzed by using a range of characterization techniques and their performances for adsorption of Cr (VI) were measured. The results indicated that K 2 C 2 O 4 facilitated cracking of aliphatic structures in WJF, diminishing AC production (25.9%) but enhancing formation of micropores (97.9% and 1108.6 m 2 g −1 ). Higher content of unstable organics in mushrooms further lowered AC yield (18.7%) but led to higher specific surface area (1417.2 m 2 g −1 ) with K 2 C 2 O 4 as an activator. ZnCl 2 promoted condensation to generate more AC (44.9%) with abundant mesopores (56.1%) from WJF. Except condensation, Maillard reactions catalyzed by ZnCl 2 retained nitrogen species in AC while cracking with K 2 C 2 O 4 transferred them into bio-oil. Although H 3 PO 4 catalyzed condensation, it could not facilitate pore development. In-situ IR characterization of activation showed that K 2 C 2 O 4 hindered aromatization while ZnCl 2 facilitated elimination of –OH and C O on nascent AC. This activated outer/inner surface of nascent AC, inducing structural re-construction and forming bulky particles of very densified structures.
研究领域:
环境科学
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材料科学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Le Wang,Zhixin Duan,Pengfei Fei,Zhifeng Yan,Wei Wang,Youbo Di,Jianjun Lu
The protease method of wool shrink-proofing finishing is challenging to apply industrially due to its low efficiency and prolonged treatment time. In this study, the activator P[(CH 2 ) n OH] 3 , n∈(1, 10) and protease Savinase 16 L were combined and used in the same bath to rapidly degrade wool scales to achieve the target of machine washable for wool. The activator significantly improved the degradation efficiency of 16 L on wool's high-sulfur scale tissue, reducing the wool shrink-proofing treatment time to 100 s. The treated wool samples' area shrinkage, strength, elasticity, length, fineness, and directional friction effect were evaluated. The results showed that the activator combined with Savinase 16 L could rapidly hydrolyze wool fiber scales, and the wool area shrinkage was 1.65 % without excessive damage to the original properties of wool fibers. Furthermore, the reaction mechanism of wool scale degradation by protease and the activator was confirmed by Raman spectroscopy, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The results showed that the activator worked by activating the wool high-sulfur scale layer, thereby increasing the efficiency of the protease degradation of wool. It had no activating effect on the protease molecule. The activator opens the disulfide bonds on the wool scales while transforming the β-thinning and β-rotation in the scales to α-helix or random curling. It could promote protease adsorption and reaction on wool. Under pH 8.0 and 50℃, 2 g/L of activator could increase the adsorption of 16 L on wool by 12.7 times and increase the hydrolysis activity of 16 L on wool by 1575 times. After treatment with this technology, the content of -NH2, -COOH, and -SOx on the surface of wool was increased. Furthermore, a continuous multiple-padding wool shrink-proofing production line was designed, including a treatment liquid circulation and heat preservation systems. Stable industrial production applications were realized.
研究领域:
纺织科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Materials
·
2021
·Vol. 15
·期 1
·271
·
IF 3.623
Woo Sung Yum,Juan Yu,Dongho Jeon,Haemin Song,Sungwon Sim,Do Hoon Kim,Jae Eun Oh
This study examined the mechanical and durability properties of CaO-activated ground-granulated blast-furnace slag (GGBFS) concretes made with three different additives (CaCl2, Ca(HCOO)2, and Ca(NO3)2) and compared their properties to the concrete made with 100% Ordinary Portland Cement (OPC). All concrete mixtures satisfied targeted air content and slump ranges but exhibited significantly different mechanical and durability properties. The CaO-activated GGBFS concretes showed different strength levels, depending on the type of additive. The added CaCl2was the most effective, but Ca(NO3)2was the least effective at increasing mechanical strength in the CaO-activated GGBFS system. The OPC concrete showed the most excellent freezing–thawing resistance in the durability test, but only the CaO-activated GGBFS concrete with CaCl2exhibited relatively similar resistance. In addition, the chemical resistance was significantly dependent on the type of acid solution and the type of binder. The OPC concrete had the best resistance in the HCl solution, while all CaO-activated GGBFS concretes had relatively low resistances. However, in the H2SO4solution, all CaO-activated GGBFS concretes had better resistance than the OPC concrete. All concrete with sulfate ions had ettringite before immersion. However, when they were immersed in HCl solution, ettringite tended to decrease, and gypsum was generated. Meanwhile, the CaO-activated GGBFS concrete with CaCl2did not change the type of reaction product, possibly due to the absence of ettringite and Ca(OH)2. When immersed in an H2SO4solution, ettringite decreased, and gypsum increased in all concrete. In addition, the CaO-activated concrete with CaCl2had a considerable amount of gypsum; it seemed that the dissolved C-S-H and calcite, due to the low pH, likely produced Ca2+ions, and gypsum formed from the reaction between Ca2+and H2SO4.Keywords:CaO-activation;auxiliary activator;GGBFS;chemical resistance;freezing;thawing
研究领域:
土木工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Xiaohan Li,Yue Zhang,Chunxiao Yu,Xu Guo,Xiangxiang Fu,Shiqi Chen,Yanguo Gao,Yu Zhang,Qin Xiang,Wei Lu,Yonghong Zhang,Qibin Wang,Li Chen,Tao Zheng
Background Scrophularia ningpoensis Hemsl. (SN) is a traditional herbal medicine used for treating diabetes mellitus (DM). Previous studies indicated that the aqueous extract of SN (AESN) improves type 2 DM (T2DM) by activating AMPK, but the active components were unclear. Purpose This study aimed to identify the compound in AESN responsible for AMPK activation and to verify its therapeutic effects on T2DM. Methods Rats were administered AESN intragastrically. The absorbed compounds in serum samples were analyzed using LC-MS/MS and subjected to molecular docking with AMPK. The compound with the highest docking score was further verified using SPR, CETSA, and TR-FRET to confirm its effects on AMPK activation. Primary mouse hepatocytes were used to examine the effects of ANC on AMPK activity, Akt/GSK3β signaling, NLRP3 inflammasome activation, and lipid accumulation. An AMPK inhibitor was used to determine whether the effects were AMPK-dependent. In type 2 diabetic db/db mice, various dosages of ANC were administered intragastrically to observe its therapeutic effects. Results Among the 17 absorbed prototype compounds, ANC exhibited the strongest binding ability to AMPK. SPR, CETSA, and TR-FRET assays confirmed that ANC binds to and activates AMPK. In hepatocytes, ANC activated the Akt/GSK3β signaling, suppressed NLRP3 inflammasome activation, and inhibited lipid accumulation in an AMPK-dependent manner. In db/db mice, ANC intervention improved glucose and lipid metabolic disorders, insulin resistance, and histopathological abnormalities in a dose-dependent manner, while activating AMPK and alleviating lipid metabolic disorders and metabolic inflammatory responses. Conclusion ANC can treat T2DM by directly binding to and activating AMPK.
研究领域:
药理学
·
代谢病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Theranostics
·
2018
·
IF 8.537
Zhenyuan Bian,Jin Yan,Simeng Wang,Yijie Li,Yi Guo,Bohan Ma,Hao Guo,Zhijie Lei,Chun Yin,Yi Zhou,Min Liu,Kaishan Tao,Peng Hou,Wangxiao He
Peptides are a rapidly growing class of therapeutics with many advantages over conventional small molecule drugs. Dextrorotary (D)-peptides, with increased enzymatic stability and prolonged plasma half-life in comparison with natural L-peptides, are considered to have great potential as recognition molecules and therapeutic agents. However, the in vivo efficacy of current therapeutic D-peptides is hindered by their inefficient cellular uptake in diseased tissues. Methods: To overcome physiological and cellular barriers to D-peptides, we designed a gold-based ultra-small nanocarrier coupled with polylysine (PLL) and a receptor-targeted peptide to deliver therapeutic D-peptides. Using a D-peptide p53 activator (DPA) as a proof of concept, we synthesized, functionalized and characterized gold- and DPA-based nanoparticles termed AuNP-DPA. Results: AuNP-DPA were effectively enriched in tumor sites and subsequently internalized by cancer cells, thereby suppressing tumor growth via reactivating p53 signaling. More importantly, through a series of in vivo experiments, AuNP-DPA showed excellent biosafety without the common side effects that hinder p53 therapies in clinic trials. Conclusion: The present study not only sheds light on the development of AuNP-DPA as a novel class of antitumor agents for drugging the p53 pathway in vivo, but also supplies a new strategy to use D-peptides as intracellular PPI inhibitors for cancer-targeted therapy.
研究领域:
生物医学
·
纳米医学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RUSSIAN CHEMICAL BULLETIN
·
2025
·Vol. 74
·期 3
·807-817
·
IF 0
Balakina A. A.,Amozova V. I.,Stupina T. S.,Mumyatova V. A.,Podgurskaya A. D.,Gadomsky S. Ya.
A new method for the synthesis of pyridoxine salts with dicarboxylic acids was proposed. The specific features of the synthesis include the preliminary generation of free pyridoxine from the hydrochloride form by treatment with a solution of triethylamine in chloroform and conduction of the reaction of pyridoxine with dicarboxylic acid in a specially selected solvent mixture. The combination of pyridoxine as a known antioxidant and fumarate as an activator of the transcription factor Nrf2 in one molecule gave rise to a unique structure, bis[3-hydroxy-4,5-bis(hydroxymethyl)-2-methylpyridinium] fumarate (DPNF). In vitro experiments showed the ability of this compound to bind superoxide, suppress lipid peroxidation processes, and behave as a mitochondrial cytoprotective agent and Nrf2 activator. The results of the study can be used to develop a new type of drugs based on activators of the transcription factor Nrf2 to protect cells and tissues from oxidative damage.
研究领域:
药物化学
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生物化学
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分子生物学
✓ 已验证
DOI
Food Bioscience
·
2025
·
IF 5.9
Tingting Li,Hongkai Tan,Tianliang Bai,Yong Wu,Xuanyi Meng,Yanhai Xie,Xin Li,Hongbing Chen
Pitcher fluid of Nepenthes × miranda is usually discarded before transportation despite containing many functional materials, in which hydrolytic proteases are particularly attractive to us. In our study, we collected the pitcher fluid of Nepenthes × miranda and obtained pitcher fluid proteases with a proteolytic activity of 6.658 U/mL. Then the optimal pH and temperature were detected, and the activators, denaturants and inhibitors were screened respectively. Finally, Michaelis constants were calculated to quantify the affinity with different substrates. The results showed peak activity at pH 2.05 to 2.50 and temperatures between 50 °C and 55 °C, which indicated an advantage of exerting proteolytic activity at lower pH levels and higher temperature compared to nepenthesins from other Nepenthes species. Moreover, the pitcher fluid proteases could be activated by Mn 2+ significantly and tolerated glycerol of high content. We discover a way of the new protease resources, a rational use for biochemistry and a direction for the reuse of agricultural waste.
研究领域:
植物学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2022
·
IF 4.177
Jingna Jia,Li Fu,Xuwen Gao,Shuangtian Dong,Yuqi Xu,Dongyang Wang,Guizheng Zou
Mechanoluminescence (ML) is one kind of mechanical-to-optical energy conversion, and a strategy toward achieving bright elastic ML emission is strongly anticipated for ML application. Herein, a ternary-host strategy is proposed for synthesizing heterojunction ML materials with eye-visible elastic ML under an ultralow pressure of 2 N, in which ZnS, CaCO3, and SrCO3 are employed as precursors to prepare the ternary-host (Ca0.5Sr0.5)ZnOS/xZnS. Upon partially substituting the precursor ZnS with MnCO3, the Mn2+ activator could introduce luminescent centers and carrier traps into the ternary-host (Ca0.5Sr0.5)ZnOS/xZnS and enable achieving a series of (Ca0.5Sr0.5)ZnOS/xZnS/Mn2+ phosphors with bright and pressure-sensitive elastic ML. The (Ca0.5Sr0.5)ZnOS/xZnS/Mn2+ phosphors not only exhibit eye-visible red ML under ambient conditions without any preirradiation but can also display a linearly enhanced ML intensity along with the enlarged external force at a 2 N interval as well as regeneratable ML upon repetitively applying an external force 20 times. The maximum emission wavelength of ML can be conveniently modulated from 623 to 658 nm by adjusting the ZnS content of (Ca0.5Sr0.5)ZnOS/xZnS/Mn2+ phosphors.
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2024
·
IF 3.8
Mingzhe Jiang,Hao Tan,Lixin Jiang,Jintang Duan,Xueping Gu,Lianfang Feng,Cailiang Zhang
The anionic ring-opening polymerization of ε-caprolactam (CL) offers significant potential for producing polyamide 6 (PA6). To establish optimal conditions for designing an efficient and continuous PA6 production process, this study examines the anionic polymerization of CL at temperatures above the crystallization point of PA6. The focus is on the effects of the temperature, activator type and concentration, and catalyst concentration on the polymerization process and product characteristics. At 220 °C, the use of bifunctional methylene diphenyl diisocyanate (MDI) as an activator initiates cross-linking reactions, producing insoluble, nonmelting PA6. In contrast, anionic polymerization without an activator prevents gel formation but results in uncontrollably high molecular weights. The introduction of monofunctional N-acetyl caprolactam (AcCL) as an activator eliminates cross-linking reactions at temperatures above 220 °C while allowing precise control of the product’s molecular weight through adjustments in AcCL concentration.
研究领域:
高分子化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Zhenyu Chen,Zhiyin Li,Ruilong Xu,Yufeng Xie,Dehuai Li,Yu Zhao
Cardiovascular diseases (CVDs) persist as the predominant cause of mortality, urging the exploration of innovative pharmaceuticals. Mitochondrial dysfunction stands as a pivotal contributor to CVDs development. Sirtuin 3 (SIRT3), a prominent mitochondrial deacetylase known for its crucial role in protecting mitochondria against damage and dysfunction, has emerged as a promising therapeutic target for CVDs treatment. Utilizing isosteviol, a natural ent-beyerene diterpenoid, 24 derivatives were synthesized and evaluated in vivo using a zebrafish model, establishing a deduced structure–activity relationship. Among these, derivative 5v exhibited significant efficacy in doxorubicin-induced cardiomyopathy in zebrafish and murine models. Subsequent investigations revealed that 5v selectively elevated SIRT3 expression, leading to the upregulation of SOD2 and OPA1 expression, effectively preventing mitochondrial dysfunction, mitigating oxidative stress, and preserving cardiomyocyte viability. As a novel structural class of SIRT3 activators with robust therapeutic effects, 5v emerges as a promising candidate for further drug development.
研究领域:
药理学
·
心血管疾病
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Xueying Lei,Lihua Ding,Xiaonan Yang,Feng Xu,Yongjun Wu,Songcheng Yu
The CRISPR/Cas12a system is increasingly used in biosensor development. However, high background signal and low sensitivity for the non-nucleic acid targets detection is challenging. Here, a p adlock activator which could i nhibit the t rans -cleavage activity of CRISPR/Cas12a system in the intact form by steric hindrance effect (PAIT effect) was designed for non-nucleic acid targets detection. The PAIT effect disappeared when padlock activator was separated into two split activators. To verify the feasibility of padlock activator, a Ca 2+ sensor was developed based on PAIT effect with the assistance of DNAzyme, activity of which was Ca 2+ dependent. In the presence of Ca 2+ , DNAzyme was activated to cleave its substrate, a padlock activator modified with adenine ribonucleotide, into split padlock activators which would trigger the trans -cleavage activity of Cas12a to generate fluorescence. There was a mathematical relationship between the fluorescence intensity and the logarithm of Ca 2+ concentration ranging from 10 pM to 1 nM, with a limit of detection of 3.98 pM. The little interference of Mg 2+ , Mn 2+ , Cd 2+ , Cu 2+ , Na + , Al 3+ , K + , Fe 2+ , and Fe 3+ indicated high selectivity. Recovery ranged from 93.32% to 103.28% with RSD s from 1.87% to 12.74% showed a good accuracy and reliability. Furthermore, the proposed sensor could be applied to detect Ca 2+ in mineral water, milk powder and urine. The results were consistent with that of flame atomic absorption spectroscopy. Thus, PAIT effect is valuable for expanding the application boundary of CRISPR/Cas12a system.
研究领域:
生物技术
·
生物化学
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Zi-Long Song,Yanan Hou,Feifei Bai,Jianguo Fang
Oxidative stress is constantly involved in the etiopathogenesis of an ever-widening range of neurodegenerative diseases. As a consequence, effective repression of cellular oxidative stress to a redox homeostatic condition is a promising and feasible strategy to treat, or at least retard the progression of, such disorders. Nrf2, a primary orchestrator of cellular antioxidant response machine, is responsible for detoxifying and compensating for deleterious oxidative stress via transcriptional activation of a diverse array of antioxidant biomolecules. In the framework of our persistent interest in disclosing small molecules that interfere with cellular redox-regulating machinery, we report herein the synthesis, optimization, and biological assessment of 47 vinyl sulfone scaffold-bearing small molecules, most of which exhibit robust neuroprotective effect against H 2 O 2 -mediated lesions to PC12 cells. After initial screening, the most potent neuroprotective compounds 9b and 9c with marginal cytotoxicity were selected for the follow-up studies. Our results demonstrate that their neuroprotective effects are attributed to the up-regulation of a panel of antioxidant genes and corresponding gene products. Further mechanistic studies indicate that Nrf2 is indispensable for the cellular performances of 9b and 9c , arising from the fact that silence of Nrf2 gene drastically nullifies their protective action. Taken together, 9b and 9c discovered in this work merit further development as neuroprotective candidates for the treatment of oxidative stress-mediated pathological conditions.
研究领域:
神经科学
·
药物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）