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SENSORS AND ACTUATORS B-CHEMICAL · 2019 · IF 6.393

A modified sensitive ELISA based on dual catalysis of Pd@Pt porous nanoparticles and horseradish peroxidase

Weiguo Wang,Rongkai Du,Changzhi Dong,Jinwu Yan,Lei Zhang

A sensitive enzyme-linked immunosorbent assay (ELISA) has been developed based on the dual catalysis of dendritic Pd-Pt porous nanoparticles and horseradish peroxidase (HRP). The Pd-Pt porous nanoparticles, for the first time, were used to immobilize HRP. As a proof-of-concept demonstration, HRP functioned Pd-Pt porous nanoparticles were applied in hCG detection with a detection limit of 0.07 IU L −1 . The linear intervals is 0.1–6.4 IU L -1 . The Pd-Pt-HRP possesses a better sensitivity than the traditional BA-ELISA. Furthermore, the precision and reproducibility and selectivity were assessed to be eligible for the hCG monitoring.

CAS / MDL: 50-81-7 7722-84-1 16921-30-5 13826-93-2 54827-17-7

研究领域: 生物化学 · 医学诊断 · 纳米技术

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Nanoscale · 2018 ·Vol. 11 ·期 3 ·962-967 · IF 7.233

Chemical pre-reduction and electro-reduction guided preparation of a porous graphene bionanocomposite for indole-3-acetic acid detection

Zhaohong Su,Xiaolin Xu,Yongbing Cheng,Yueming Tan,Langtao Xiao,Daili Tang,Hongmei Jiang,Xiaoli Qin,Huixian Wang

A porous graphene (PG) bionanocomposite of PG, gold nanoparticles (AuNPs) and anti-indole-3-acetic acid (anti-IAA) antibody for sensitive and label-free amperometric immunoassay of IAA was reported. A PG film was produced by a pre-reduction/electrochemical reduction process on a glassy carbon electrode (GCE) and then a homogeneous AuNPs layer electrodeposition on the PG film. The anti-IAA antibody was immobilized onto the AuNPs through electrostatic adsorption and covalent conjugation. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), elecro-chemical impedance spectroscopy (EIS), ultraviolet visible spectroscopy (UV-vis) and differential pulse voltammetry (DPV) were used to characterize the PG film and the stepwise modification of the immunosensor. The electrochemical immunosensor exhibited a wide linear range from 2 × 10−11 to 2 × 10−8 g mL−1 with a detection limit of 0.016 ng mL−1 (S/N = 3) and showed significant linearity R2 = 0.9970. In addition, the proposed immunosensor showed acceptable selectivity and has been applied to the determination of IAA in the extract samples of several plant seeds with acceptable relative derivation (%) ranging from −5.25% to 4.24% between the immunosensor and high performance liquid chromatography. The proposed chemical pre-reduction and electro-reduction guided protocol can be extended to the preparation of many other functionalized PG nanocomposite films for wide applications.

研究领域: 生物传感 · 纳米生物技术 · 分析化学 · 植物生理学 · 电化学 · 免疫分析

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A modified sensitive ELISA based on dual catalysis of Pd@Pt porous nanoparticles and horseradish peroxidase

Chemical pre-reduction and electro-reduction guided preparation of a porous graphene bionanocomposite for indole-3-acetic acid detection