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Agriculture-Basel · 2019 ·Vol. 9 ·期 12 ·250 · IF 0

Chemical Composition of the Cuticle Membrane of Pitaya Fruits (Hylocereus Polyrhizus)

Hua Huang,Yueming Jiang

This study comprehensively analysed the chemical composition of the cuticle in pitaya fruits. The total coverage amount of the waxes versus cutin monomers accumulated at a ratio of 0.6, corresponding to masses per unit of 30.3 μg·cm−2and 50.8 μg·cm−2, respectively. The predominant wax mixtures weren-alkanes in homologous series of C20–C35, dominated by C31and C33; as well as triterpenoids with an abundant amount of uvaol, lupenon, β-amyrinon, and β-amyrin. The most prominent cutin compounds were C16- and C18-type monomers, in which 9(10),16-diOH-hexadecanoic acid and 9,10-epoxy-ω-OH-octadecanoic acid predominated, respectively. The average chain length (ACL) of aliphates in pitaya fruit cuticle (30.5) was similar to that estimated in leaf waxes, and higher than that in most of the fruit and petal waxes that have been reported. We propose that the relatively high ACL and wax/cutin ratio might enhance the cuticular barrier properties in pitaya fruit cuticle to withstand drought.Keywords:pitaya fruit;cuticle;wax;cutin;ACL

CAS / MDL: 110-86-1
研究领域: 植物科学 · 农业化学
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS · 2024 · IF 4.9

Synthesis of dragonfruit-based silicon nanoparticles at room temperature and their application in the real-time fiber-optic detection of doxorubicin

Xiaoming Chen,Shun Liu,Mo Shen,Zhipeng Song,Sudu Bilige,Jingkang Li,Yueti Li,Chungang Wu,Liangliang Sun,Jishun Shi,Zihao Chen,Yong Zhao

Doxorubicin (DOX) has been extensively used in cancer chemotherapy as an antibiotic drug, but when DOX concentration exceeds the threshold value, irreversible liver toxicity and cardiotoxicity can be triggered. Therefore, it is a significant theme to detect DOX concentration of patient blood and metabolites for the optimization of drug dosage. Linear correlation between fluorescence quenching of silicon nanoparticles (SiNPs) and DOX concentration enables SiNPs to present good prospect to address this tricky issue. Inspired by these aspects, we put forward a novel method to synthesize dragonfruit-based SiNPs (DSiNPs) at room temperature, and leverage them in the fiber-optic sensing of urine DOX. Firstly, we investigate a simple and green synthesis method of DSiNPs emitting blue-green fluorescence through a one-step redox reaction at room temperature with organosilane as silicon source and fresh dragonfruit juice as reducing agent. Secondly, depending on optical characterization, we demonstrate mechanism of DSiNPs in the detection of DOX. Thirdly, a fiber-optic sensor is used to measure the fluorescence intensity of DSiNPs under diverse salt concentrations, pH values, and illumination time to explore the tolerance of DSiNPs. Fourthly, based on optimization of parameters and validation of specificity, we acquire linear response range of this method to DOX from 0 to 50 μM with detection limit of 3 μM. Finally, this method is leveraged in the detection of urine DOX, and obtained the recovery rates of 92.30 % and 107.30 %. This method holds excellent potential in the clinical analysis of anti-tumor drugs for the advantages of environmental friendliness and simple operation.

研究领域: 生物医学工程 · 药物分析 · 纳米材料科学
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SEPARATION AND PURIFICATION TECHNOLOGY · 2022 · IF 9.136

Oxygen self-doping formicary-like electrocatalyst with ultrahigh specific surface area derived from waste pitaya peels for high-yield H2O2 electrosynthesis and efficient electro-Fenton degradation

Shaolong Wang,Hao Liu,Dingding Ye,Qiao Lan,Xun Zhu,Yang Yang,Rong Chen,Qiang Liao

Both sustainable storage of renewable energy using H 2 O 2 as energy carrier and wastewater treatment by electro-Fenton are highly dependent on the efficiency of H 2 O 2 electrosynthesis via 2-electron reduction of O 2 . Rational design of high-performance and cost-effective electrocatalysts with facile preparation is bear the brunt of scalable H 2 O 2 electrosynthesis. Herein, for the first time, we synthesize a pitaya peel-derived formicary-like electrocatalyst employing facile pyrolysis and KOH activation. At the optimal mass ratio of biochar to KOH (1:3), the electrocatalyst exhibits prominent H 2 O 2 activity and selectivity owing to its rich self-doped oxygen-containing functional groups as well as structural defects and sp 3 -C bonds serving as reactive sites, rational hierarchical pores boosting mass transfer of reactants and products, and ultrahigh specific surface area facilitating the exposure of reactive sites. Under natural air diffusion, the pitaya peel-derived catalyst-coated cathode achieves surprising H 2 O 2 productivity of 41.6 mg h −1  cm −2 with current efficiency of 65.5% at large current density of 100 mA cm −2 , meeting the near-industrial requirements for sustainable H 2 O 2 production. Long-term operation stability at large current and rapid degradation of multiple high-concentration organic pollutants demonstrate tremendous potential of the pitaya peel-derived catalysts for H 2 O 2 -based renewable energy storage and environmental remediation.

研究领域: 材料科学 · 环境科学 · 能源科学
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POSTHARVEST BIOLOGY AND TECHNOLOGY · 2024 · IF 6.4

Differential changes in cuticular wax affect the susceptibility to fruit decay in pitaya after harvest: A cultivar comparative study

Hua Huang,Xuemei He,Qingming Sun,Guoming Liu,Yayuan Tang,Jian Sun

Pitaya ( Hylocereus undatus ) fruit is susceptible to pathogens that induce fruit decay after harvest. Cuticular waxes covering the outermost plant surfaces are the first site of plant-environment interactions; however, their changes in response to fruit decay are under-researched. Herein, the surface microstructures and chemical composition of wax on pitayas, as well as their changes in response to fruit decay, were comparatively studied. The varieties ‘Baishuijing’ (BSJ) and ‘Hongshuijing’ (HSJ) were examined after harvest. The fruit appearance, morphology, and wax composition differed between cultivars. The surface of both pitaya cultivars was smooth, although traces of granules and a thicker lipid layer were observed using histochemical staining in HSJ than in BSJ, as well as a higher cuticular wax content. Fruit decay rate increased rapidly in both cultivars under room storage conditions, and greater sensitivity to pathogens was found in BSJ than HSJ. As well as the occurrence of fruit decay, the total wax coverage, very-long-chain primary alcohols, aldehydes, fatty acids, and triterpenoids decreased in BSJ, while remaining relatively stable in HSJ. Correspondingly, the relative gene expression levels of HuKCS11 (−1, 2, and 3) , HuKCS17 , and HuKCS19 (for the elongation of very-long-chain fatty acids), HuCER1 (for aldehydes or n -alkanes), HuFAR (−1 and 2) (for primary alcohols), HuBAS1 (−1, 3, and 4) (for β-amyrin), and HuLUS1 (for lupeol) were considerably modified following fruit senescence during storage. The difference in surface topography and wax accumulation between cultivars might have affected pathogen susceptibility after harvest. These results may help when choosing varieties of pitaya based on surface characteristics and cuticular chemicals. They may also help to optimize strategies for postharvest preservation and storage.

CAS / MDL: 6368-72-5
研究领域: 植物科学 · 农学 · 采后生理学 · 分子生物学
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Foods · 2025 ·Vol. 14 ·期 15 ·2708 · IF 5.1

Hylocereus polyrhizus Pulp Residues Polysaccharide Alleviates High-Fat Diet-Induced Obesity by Modulating Intestinal Mucus Secretion and Glycosylation

Guanghui Li,Kit-Leong Cheong,Yunhua He,Ahluk Liew,Jiaxuan Huang,Chen Huang,Saiyi Zhong,Malairaj Sathuvan

AlthoughHylocereus polyrhizuspulp residues polysaccharides (HPPP) have shown potential in improving metabolic disorders and intestinal barrier function, the mechanism by which they exert their effects through regulating O-glycosylation modifications in the mucus layer remains unclear. Therefore, this study established a HFD-induced obese colitis mouse model (n= 5 per group) and combined nano-capillary liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) technology to quantitatively analyze the dynamic changes in O-glycosylation. Additionally, through quantitative O-glycosylation proteomics and whole-proteome analysis, we identified 155 specifically altered O-glycosylation sites in colon tissue, with the glycosylation modification level of the MUC2 core protein increased by approximately 2.1-fold. The results indicate that HPPP alleviates colonic mucosal damage by regulating interactions between mucus O-glycosylation. Overall, we demonstrated that HPPP increases HFD-induced O-glycosylation sites, improves intestinal mucosal structure in obese mice, and provides protective effects against obesity-induced intestinal mucosal damage.

CAS / MDL: 64-17-5
研究领域: 营养科学 · 生物化学 · 分子生物学 · 食品科学 · 胃肠病学
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ANALYTICAL LETTERS · 2020 · IF 1.467

Determination of carbendazim by aptamer-based fluorescence resonance energy transfer (FRET)

Xinyue Zhao,Xiaomeng Zhang,Mingwei Qin,Yuzhu Song,Jinyang Zhang,Xueshan Xia,Xiuming Cui,Kai Gao,Qinqin Han

Because of diverse adverse effects of carbendazim (CBZ) upon the human body and environment, a simple method for the determination of this molecule is urgently needed. Therefore, a method based on an aptamer was developed. An aptamer of carbendazim, CZ12, labeled with a fluorescent molecule (FAM), was adsorbed on graphene oxide (GO) by π-π stacking. The fluorescence resonance energy transfer (FRET) between the molecule and GO was used to rapidly quench the fluorescence signal. In the presence of carbendazim, the fluorescent molecule selectively combines with carbendazim, thereby greatly reducing the binding efficiency of the former with GO. Thus, by mixing a FAM-labeled carbendazim aptamer, GO, and food samples, the carbendazim content was determined from the fluorescence measurements. The limit of quantification was 10.0 ng/mL for strawberry, banana, pitaya, and Panax notoginseng samples. The linear range was from 0 to 125 ng/mL with a linear correlation coefficient equal to 0.9918.

CAS / MDL: 82-68-8 23564-05-8
研究领域: 分析化学 · 食品科学 · 环境科学
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FOOD CHEMISTRY · 2021 · IF 7.514

Improving drying kinetics, physicochemical properties and bioactive compounds of red dragon fruit (Hylocereus species) by novel infrared drying

Edidiong Joseph Bassey,Jun-Hu Cheng,Da-Wen Sun

Effects of tray rotation speeds (TRS: 0, 20, 40 rpm), temperatures (50, 60, 70 °C) and wavelength spectra (mid and near-infrared) were comparatively evaluated on improving drying kinetics, physicochemical properties and bioactive content of red dragon fruits. Results indicated that successive increases in TRS and temperature led to significant reductions in drying time and increases in drying rates and moisture diffusivity. High TRS (40 rpm) and lower temperatures (50, 60 °C) also improved colour, total soluble solids, rehydration ratio, total phenolics and flavonoid contents, betalain content and antioxidant activity. Meanwhile, NIR drying presented a more energy-efficient approach, but with substantial reductions in quality properties compared with MIR drying. Overall, the results suggested the importance of wavelength absorption properties of plant tissues and potential avoidance of localized overheating for enhanced efficiency during infrared drying and prompted the development of suitable approaches and optimization studies for improving efficiency.

CAS / MDL: 117-39-5
研究领域: 食品科学 · 农业工程 · 采后技术
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FOOD HYDROCOLLOIDS · 2025 · IF 12.4

A pH responsive fluorescent/visible light-emitting film for real time visual monitoring of food freshness

Chang Liu,Ning Li,Linjie Luo,Xu Li,Zhiming Liu

A dual-layer pH-responsive fluorescent film was developed using hydroxypropyl methylcellulose (HPMC), zinc alginate (ZA), fluorescein isothiocyanate (FITC), and dragon fruit peel extract (DE) for real-time food freshness monitoring. The outer protective layer served as a hydrophobic barrier to reduce moisture interference, while the inner indicator layer acted as a pH-sensitive interface, integrating FITC's fluorescence and DE's visible color change for dual-mode sensing. The film exhibited good thermal stability, antimicrobial activity, and improved hydrophobicity. Optical responses were evaluated across a wide pH range (2.0–12.0), showing stable visible color under ambient light and enhanced fluorescence under 365 nm UV. Upon exposure to ammonia vapor, fluorescence intensity increased linearly from 0 to 1000 ppm (R 2  = 0.9895; LOD = 6.73 ppm), while visible color changes became evident at 500–5000 ppm, with R/B and R/G ratios exhibiting strong linearity (R 2  = 0.96176 and 0.96747; LODs = 1126 ppm and 958 ppm, respectively). Application trials with pork revealed distinct color changes after 4 days at 25 °C or 2 days at 5 °C. These results confirm the film's effectiveness for dual-mode, non-destructive, and real-time monitoring of pork freshness.

研究领域: 食品科学 · 材料科学 · 传感技术 · 包装工程 · 分析化学
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ULTRASONICS SONOCHEMISTRY · 2022 · IF 9.336

Thermoultrasound and microwave-assisted freeze-thaw pretreatments for improving infrared drying and quality characteristics of red dragon fruit slices

Edidiong Joseph Bassey,Jun-Hu Cheng,Da-Wen Sun

The effects of thermoultrasound (US-FT), microwave (MW-FT), and room temperature (RT-FT) freeze–thaw pretreatments were evaluated for improving drying kinetics and quality during infrared drying (IRD) of red dragon fruit slices (RDFS). Results indicated that microstructural alterations induced by the different pretreatments improved the moisture removal rate and effective diffusivity, and significantly reduced the drying time. US-FT pretreatments prompted more efficient drying and presented an overall enhancement in the quality of RDFS, particularly at low temperatures of 25 and 50 ℃, while enhancements in TFC, FRAP, and CUPRAC were associated with RT-FT. High-power (500 W) MW-FT pretreatments improved colour and structural properties, while low-power (100, 300 W) improved TPC, TFC, ascorbic acid, betalains, and antioxidant activity. Overall, cellular and chemical alterations prompted by pretreatments improved the drying process but presented adverse effects on betaxanthin. The study presented the fundamental background for improving the IRD of foods from the use of improved thawing approaches during freeze–thaw pretreatments.

研究领域: 食品科学 · 农业工程 · 采后技术 · 干燥工艺
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FOOD HYDROCOLLOIDS · 2021 · IF 9.147

Protective effects of six different pectic polysaccharides on DSS-induced IBD in mice

Dongmei Wu,Shiguo Chen,Xingqian Ye,Shokouh Ahmadi,Weiwei Hu,Chengxiao Yu,Kai Zhu,Huan Cheng,Robert J. Linhardt,Qiaojun He

Administration of pectic polysaccharides is a therapeutic strategy in the management of gut inflammation. However, the knowledge about the effects of different dietary sources and structure are missing. This study explored the protective effects of six different pectic polysaccharides from Goji berry (G), dragon fruit (D), lemon (L), pomelo (P), potato (T) and raspberry (R) in dextran sulfate sodium (DSS)-induced colitis mice. D mainly contained rhamnogalacturonan (RG) backbone; L had high amount of arabinan sidechains; P was a high-esterified homogalacturonan (HG) pectic polysaccharide; the primary domain in T was galactan sidechains; R and G had the composition of arabinan: galactan: RG: HG = 19: 28: 15: 22 and 36: 17: 21: 14. Our results suggested that G and R were the most effective in alleviating colitis via alleviating tissue damage, promoting the index of immune organs, suppressing the production of TNF-α, IL-1β and IL-17, improving antioxidant status and promoting the total amount of SCFAs. Similarly, the administration of L alleviated colitis mainly by increasing the fermentation products without changes in the composition pattern of SCFAs. The secretion of IL-6 might be suppressed by pectic polysaccharides containing high amount of arabinan sidechains, while anti-inflammatory cytokines and intestinal barrier function tend to be regulated by pectic polysaccharides rich in galactan. In conclusion, pectic polysaccharide containing diversified structural domains (homogeneous proportion of HG, RG, arabinan, and galactan) might be the most effective in alleviating gut inflammation. This unprecedented report revealed the anti-inflammatory effects of different pectic polysaccharides, which was critical for the reasonable selection of dietary fiber to prevent gut inflammation.

CAS / MDL: 119-90-4
研究领域: 营养学 · 免疫学 · 胃肠病学 · 药理学
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