JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Zi-Jian Chen,Hui-Ling Wu,Yu-Dong Shen,Hong Wang,Yi-Feng Zhang,Bruce Hammock,Zhen-Feng Li,Lin Luo,Hong-Tao Lei,Zhen-Lin Xu
The excessive use of carbaryl has resulted in the risk of its exposure. In this study, we isolated six nanobodies (Nbs) from a camelid phage display library against the biomarker of carbaryl, 1-naphthol (1-NAP). Owing to its characteristics of easy genetic modifications, we produced a nanobody-alkaline phosphatase (Nb-CC4-ALP) fusion protein with good stability. A dual-emission system based ratiometric fluoroimmunoassay (RFIA) for quick and highly sensitive determination of 1-NAP was developed. Silicon nanoparticles (SiNPs) was used as an internal reference and for aggregation-induced emission enhancement (AIEE) of gold nanoclusters (AuNCs), while AuNCs could be quenched by MnO 2 via oxidation . In the presence of ALP, ascorbic acid phosphate (AAP) can be transformed into ascorbic acid (AA), the later can etch MnO 2 to recover the fluorescence of the AuNCs. Based on optimal conditions, the proposed assay showed 220-fold sensitivity improvement in comparison with conventional monoclonal antibody-based ELISA. The recovery test of urine samples and the validation by standard HPLC-FLD demonstrated the proposed assay was an ideal tool for screening 1-NAP and provided technical support for the monitoring of carbaryl exposure.
研究领域:
环境科学
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分析化学
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毒理学
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DOI
PubMed
高影响因子
Materials Today Chemistry
·
2025
·
IF 6.7
Hongmei Liu,Boyue Zheng,Zhengxue Ge,Mengyun Liu,Caihong Tao,Fangbin Fan
The analysis and treatment of hazardous substances in food have long been constrained by the dual challenges of limited single-target recognition capability and low resource utilization efficiency. To overcome these limitations, this study developed an ionic liquid-hydrogel material using a polymerization technique. The material featured a stable porous nanostructure and exhibited dual functionality, enabling highly efficient photo-catalytic degradation and adsorptive extraction. Under optimized conditions, it achieved a degradation rate of over 90 % for the dye Amaranth and extraction recovery exceeding 85 % for three benzoylurea pesticides. Mechanistic studies using in-situ IR spectroscopy revealed that the material's efficient and synergistic pretreatment capability for the two distinct classes of pollutants was attributed to the high substrate specificity of its active sites, which ensured that the two pathways operated independently without interference, guaranteeing the reliability of the treatment process. In conclusion, the developed dual-functional material combining with the advantages of low cost, ease of preparation and environmental friendliness provided an innovative strategy for the simultaneous pretreatment of multiple food contaminants and represented a significant advancement in this field.
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高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Yuecheng Zhao,Lu Zhang,Chuanshan Zou,Huilin Han,Chengde Li,Xingpeng Li,Liwen Song
BACKGROUND Cuticular proteins (CPs) play essential roles in forming cuticular structures in insects. However, the specific functions and regulatory mechanisms of CPs remain largely unexplored. In this study, the Larval cuticular protein 17 ( HcLCP-17 ) gene was identified from Hyphantria cunea , a highly destructive and polyphagous forest pest. To investigate the role of HcLCP-17 in cuticular function and transcriptional regulation mediated by 20E-responsive transcription factors (ERTFs), we employed RNA interference (RNAi) and yeast one-hybrid assay techniques. Additionally, we examined the molecular mechanism by which chlorbenzuron, a type of benzoylphenylurea (BPU) that functions as a chitin synthesis inhibitor (CSI), affects the 20E signaling pathway and ultimately regulates HcLCP-17 expression. RESULTS HcLCP-17 encodes a polypeptide consisting of 393 amino acids, which includes a chitin-binding domain. Silencing HcLCP-17 resulted in a disturbance in the structural organization of the larval cuticle and a notable reduction in chitin levels. HcLCP-17 expression was controlled by the interaction between Broad-Complex (Br-C) and beta Fushi Tarazu Factor-1 (βFTZ-F1) with its promoter fragment. Furthermore, the inhibitory effect of chlorbenzuron on HcLCP-17 expression was found to be potentially mediated by Br-C and βFTZ-F1. CONCLUSION The study presents a novel mode of action for the 20E signaling pathway in regulating the expression of CPs and reveals the potential mode-of-action of BPUs in insect cuticles. These findings provide a theoretical basis for future utilization of LCP-17 as a pesticide target making a significant contribution to the development of effective pest management strategies. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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害虫管理
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2024
·Vol. 47
·期 11
·2300730
·
IF 2.8
Hongmei Liu,Baogui Ye,Zhongwei Zhao,Mengyun Liu,Fangbin Fan,Caihong Tao
A fast and effective analytical method with biomass solid-phase microextraction sorbent combined with a high-performance liquid chromatography-ultraviolet detector was proposed for the determination of benzoylurea (BU) insecticides in tea products. The novel sorbent was prepared by activating and then carbonizing water hyacinth with a fast growth rate and low application value as raw material and showed a high specific surface area and multiple interactions with analytes, such as electrostatic action, hydrogen bonding, and π-π conjugation. After optimizing the three most important extraction parameters (pH [X 1 ], sample loading rate [X 2 ], and solution volume [X 3 ]) by Box-Behnken design, the as-established analytical method showed good extraction performance: excellent recovery (80.13%–106.66%) and wide linear range (1–400 µg/L) with a determination coefficient of 0.9992–0.9999, a low limit of detection of 0.02–0.1 µg/L and the satisfactory practical application results in tea products. All these indicate that the water hyacinth-derived material has the potential as a solid-phase extraction sorbent for the detection and removal of BU insecticides from tea products, and at the same time, it can also achieve the effect of rational use of biological resources, maintaining ecological balance, turning waste into treasure, and achieving industrial production.
研究领域:
环境科学
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分析化学
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植物学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2025
·
IF 4
Xuejing Lou,Yingge Hao,Huina Zhou,Zhen Song,Youmei Wang,Minghua Lu
The creation of defects in crystalline structures can tune metal-organic frameworks (MOFs) properties, such as improving their adsorptive and catalytic performance with producing more porosity and active sites. In this work, the bimetallic UiO-66 containing Zn and Zr was prepared. And then UiO-66 with missing cluster defects (UiO-66-1/3) were obtained by acid washing to remove the Zn nodes. UiO-66-1/3 was used as sorbent of dispersive solid-phase extraction (dSPE) to extract and enrich (BUs). Combination with high-performance liquid chromatography-diode array detector (HPLC-DAD) was developed to detect trace BUs in soil samples. Adsorption equilibrium can be reached in 3 min. The method possesses high enrichment factor (202-325), low detection limit (0.005-0.04 ng·mL -1 ), and wide linear range (0.02-200 ng·mL -1 ). In addition, the recovery rate of UiO-66-1/3 as an adsorbent was still higher than 95% after reused for 16 times. This work provides a new material for the enrichment and detection of benzoylurea insecticides in the environment.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
Polymer-Plastics Technology and Materials
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2024
·
IF 2.6
Zhenkun Chen,Tian Feng,Xiaomin Cheng
A novel multifunctional silica magnetic molecularly imprinted polymer (MQDs-MIPs) nanoparticle probe was synthesized for the rapid detection of triflumuron (TFM). The fluorescence probe consists of magnetite (Fe3O4) as a core and is wrapped with a molecularly imprinted polymer doped with Mn-doped ZnS quantum dots (QDs). Under optimal detection condition, the fluorescence quenching efficiency of MIP@Fe3O4@ZnS:Mn QDs displayed a linear relationship over the concentration range of 0–27.88 μmol/L with a correlation coefficient of 0.9947. Four structurally similar compounds were selected for selectivity testing and the nanocomposite showed good selectivity with a higher imprint factor of 2.52. This developed method has the advantages of simple preparation, fast response and low toxicity for trace TFM detection.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 32
·12563-12569
·
IF 3.069
Huixiao Duo,Xiaofeng Lu,Shuai Wang,Licheng Wang,Yong Guo,Xiaojing Liang
Herein, a novel, fusiform-like magnetic metal–organic framework material (Fe3O4-NH2@MOF-235) was fabricated by a facile two-step solvothermal approach. Then, Fe3O4-NH2@MOF-235 was applied as an adsorbent in magnetic solid-phase extraction (MSPE) coupled with high-performance liquid chromatography (HPLC) for the simultaneous extraction and determination of five kinds of benzoylurea insecticides (flufenoxuron, chlorbenzuron, teflubenzuron, diflubenzuron and triflumuron) from honey, fruit juice and tap water samples. Moreover, potential variables influencing the MSPE efficiency were optimized. Under the optimized conditions, an excellent linearity was achieved in the range of 1.0–300.0 μg L−1 for teflubenzuron and flufenoxuron and 2.0–300.0 μg L−1 for diflubenzuron, chlorbenzuron and triflumuron, with the correlation coefficients of 0.9991–0.9995. The limits of detection for the analytes were 0.25–0.5 μg L−1, and the recoveries at three fortified concentration levels ranged from 76.45% to 90.74% for the honey samples, 79.95–95.27% for the fruit juice samples, and 84.02–99.62% for the tap water samples. The π–π stacking and hydrophobic interactions between Fe3O4-NH2@MOF-235 and the analytes played an important role in the adsorption process. Moreover, maximum adsorption could be achieved within 5 minutes with very little adsorbent for all the samples. Therefore, the magnetic Fe3O4-NH2@MOF-235 composite combined with MSPE is a convenient approach to detect benzoylurea insecticides in actual samples.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
·
IF 3.571
Yong Han,Weixia Yang,Xueli Luo,Xie He,Ying Yu,Chunhua Li,Wenzhi Tang,Tianli Yue,Zhonghong Li
Copper ion (Cu2+)-triggered carbon dots (CDs/Cu) with dual emissions were utilized to develop a ratiometric fluorescence sensor for ultrasensitive detection of inert thiophanate-methyl (TM). TM could be recognized by CDs/Cu through π–π stacking and could chelate Cu2+ of CDs/Cu through metal ion coordination, inducing synchronous fluoresent quenching of the dual emission of CDs/Cu based on the excited state intramolecular proton transfer and the ligand-to-metal charge transfer. The fluorescence ratio of CDs/Cu (F416/F481) linearly responded in a TM concentration of 0.10–20.00 μmol/L with an ultralow limit of detection of 2.90 × 10–6 μmol/L. A synchronous response of the ratiometric sensor enhanced the specificity toward TM and presented remarkable capability of anti-interference in complex matrices. The sensor exhibited satifactory accuracy and precision for practical applications with recoveries of 88.33–101.09% and relative standard deviations of 1.61–5.06%, demonstrating an ultrasensitive ratiometric fluorescent nanosensor for detecting pesticides residues in complex matrices.
研究领域:
分析化学
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环境科学
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食品安全
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 1
·1-9
·
IF 6.232
Duo Huixiao,Li Yijing,Liang Xiaojing,Wang Shuai,Wang Licheng,Guo Yong
Magnetic hierarchical nickel/nickel oxide/carbon nanorods (Ni/NiO@C) were prepared via the pyrolysis of a metal-organic framework containing nickel(II) nickel (Ni-MOF; Ni 3 (BTC) 2 ) under argon atmosphere. In this material, magnetic Ni/NiO@C nanoparticles are embedded in porous carbon nanorods, and the morphology is similar to that of the original Ni-MOF precursor. The synthesized nanorods were applied as magnetic sorbents in the solid-phase extraction of five benzoylurea insecticides (flufenoxuron, chlorbenzuron, teflubenzuron, diflubenzuron and triflumuron), and their performance was evaluated under optimized conditions. The results show that the material exhibits high extraction recoveries from spiked samples (82.9%–107.6%) and linear response in the range of 0.2–450 μg·L −1 . It is also characterized by relatively low limits of detection (50–100 ng·mL −1 ) at a signal-to-noise ratio of 3. The sorbent is chemically stable and can be repeatedly recycled, with little decline in extraction capacity after 20 cycles of reuse. The method was successfully applied to the quantification of benzoylureas in tea, wolfberry, millet, and oat samples, and it showed high extraction efficiency.
Schematic representation of the synthesis of magnetic hierarchical nickel/nickel oxide/carbon nanorods derived from Ni-MOF. The material is employed as a sorbent for the magnetic solid-phase extraction of benzoylurea insecticides.
研究领域:
分析化学
·
环境科学
·
农药残留检测
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DOI
PubMed
高影响因子
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·Vol. 99
·期 4
·e21512
·
IF 1.316
Guang-Wei Li,Xiu-Lin Chen,Xiang-Li Xu,Jun-Xiang Wu
Insect antennae have a primary function of perceiving and discerning odorant molecules including sex pheromones and host plant volatiles. The assumption that genes highly expressed in the antennae may have an olfactory-related role associated with signal transduction. Here, one delta subfamily glutathione S -transferase (GST) gene (GmolGSTD1) was obtained from an antennal transcriptome of Grapholita molesta . Quantitative real-time polymerase chain reaction results revealed that GmolGSTD1 was mainly expressed in antennae and the expression levels were significantly higher in female antennae than in male antennae. The recombinant GmolGSTD1 (rGmolGSTD1) showed glutathione-conjugating activity toward 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. The pH range for optimal rGmolGSTD1 enzyme activity was 6.0–6.5, and rGmolGSTD1 enzyme activity had maximal peaks at 35–40°C. Spectrophotometric analysis indicated that insecticides had weak inhibitory effects on the activity of rGmolGSTD1 with the inhibitory rates of 28.82% for chlorpyrifos, 22.27% for lambda-cyhalothrin, 18.07% for bifenthrin, 20.42% for acetamiprid, 17.57% for thiamethoxam, 25.67% for metaflumizone, 27.43% for abamectin, and 7.24% for chlorbenzuron. rGmolGSTD1 exhibited high degradation activity to the sex pheromone component (Z)-8-dodecenyl alcohol and the host plant volatile butyl hexanoate with the degradation efficiency of 75.01% and 48.54%, respectively. We speculate that GmolGSTD1 works in inactivating odorant molecules and maintaining sensitivity to olfactory communication of G. molesta .
研究领域:
昆虫学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 33
·3153-3159
·
IF 3.532
Ling Mei,Tiantian Si,Shuai Wang,Jie Zhu,Hao Tang,Xiaojing Liang
The TiO2@MOF-919(Fe–Cu) solid-phase extraction material was prepared by growing MOF-919(Fe–Cu) in situ on three-dimensional radial TiO2 microspheres by a simple solvothermal method. This combination drew on both the resources of good single dispersion and extraction rate, which made it a better extraction material. It was accompanied with high-performance liquid chromatography (SPE–HPLC) for the separation and determination of four benzoylurea pesticides (triflumuron, chlorbenzuron, teflubenzuron and diflubenzuron) in afforestation irrigation water and juice samples (grape, peach and apple juices). Under the optimal conditions, the linearity of the method ranged from 1 to 400 μg L−1 with a correlation coefficient (R2) ≥ 0.9994, while the detection limit was in the range of 0.40–0.56 μg L−1 for the four pesticides. The adopted material showed good reusability and can be used no less than 10 times. The intra-day and inter-day precision were in the range of 1.78–3.24% and 4.06–5.08%, respectively. The proposed method was then successfully applied for the detection of benzoylurea pesticides in the spiked samples with good recoveries (72.3–108.4%) and good precision (5.15%) due to π–π and hydrophobic interactions between the analytes and adsorbent. The results show that the composite had the potential to be used as a SPE adsorbent for the enrichment and extraction of benzene ring structures containing imide groups in actual samples.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Xueli Luo,Gengli Huang,Yuchen Li,Jie Guo,Xiaokai Chen,Youwen Tan,Wenzhi Tang,Zhonghong Li
Ultrasensitive, reliable and prompt pre-warning of pesticide residue is critically significant to ensure environmental and food safety. Herein, a novel nanosensor fabricated by in situ encapsulating N -doped carbon dots into a porphyrin metal–organic framework was developed to ratiometric fluorescence (RF) and smartphone-integrated portable platform colorimetric dual-mode detection of glyphosate (GLP). The sensor responded to GLP by RF in the range of 0.01–6.67 mg/L with low limit of detection of 9.06 µg/L. Besides, a noticeable color evolution from pink purple to green under nature light was observed upon addition of GLP, thus a portable smartphone-integrated colorimetric platform was fabricated for visual colorimetric detecting GLP by incorporating the sensor with a smartphone and a 3D-printed mini-device. Interestingly, the platform can detect GLP merely by virtue of a built-in flashlight of the smartphone and Color Analyser Application. Additionally, desirable recoveries of 94.0–112.5 % and 90.0–113.6 % in spiked real samples were respectively obtained for RF and colorimetric channel. Furthermore, a one-to-one colorimetric logic gate (RGB vs GLP concentrations) offered the possibility of intelligent monitoring of GLP. Summarily, the versatile sensor satisfied laboratory instrumental analysis and was further integrated as a smartphone-assisted platform to furnish a novel tactic for quick GLP monitoring, particularly for resource-constrained settings.
研究领域:
环境科学
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食品安全
·
分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）